A Rapid and Quantitative Flow Cytometry Method for the Analysis of Membrane Disruptive Antimicrobial Activity

We describe a microbial flow cytometry method that quantifies within 3 hours antimicrobial peptide (AMP) activity, termed Minimum Membrane Disruptive Concentration (MDC). Increasing peptide concentration positively correlates with the extent of bacterial membrane disruption and the calculated MDC is...

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Veröffentlicht in:	PloS one 2016-03, Vol.11 (3), p.e0151694
Hauptverfasser:	O'Brien-Simpson, Neil M, Pantarat, Namfon, Attard, Troy J, Walsh, Katrina A, Reynolds, Eric C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acinetobacter baumannii Anti-Bacterial Agents - pharmacology Anti-infective agents Antibiotics Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Antimicrobial Cationic Peptides - pharmacology Apoptosis Assaying Bacteria Bacterial infections Biology and Life Sciences Cell Membrane - drug effects Cell survival Cytometry Dental schools Drug resistance E coli Escherichia coli Escherichia coli - drug effects Flow cytometry Flow Cytometry - methods Gram-negative bacteria Gram-Negative Bacteria - drug effects Gram-Positive Bacteria - drug effects High-Throughput Screening Assays - methods Influence Lipids Medicine and Health Sciences Membranes Membranes (Biology) Methicillin-Resistant Staphylococcus aureus - drug effects Microbial Sensitivity Tests - methods Microorganisms Minimum inhibitory concentration Multidrug resistance Nosocomial infections Oral hygiene Pathogens Peptides R&D Research & development Research and Analysis Methods Staphylococcus aureus Staphylococcus infections Streptococcus infections
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