The Effect of Probiotic Treatment on Patients Infected with the H7N9 Influenza Virus
A novel avian-origin influenza A (H7N9) virus emerged and spread among humans in Eastern China in 2013. Prophylactic treatment with antibiotics and probiotics for secondary infection is as important as antiviral treatment. This study aims to assess the ability of probiotic treatment to restore inter...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0151976-e0151976 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0151976 |
|---|---|
| container_issue | 3 |
| container_start_page | e0151976 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Hu, Xinjun Zhang, Hua Lu, Haifeng Qian, Guirong Lv, Longxian Zhang, Chunxia Guo, Jing Jiang, Haiyin Zheng, Beiwen Yang, Fengling Gu, Silan Chen, Yuanting Bao, Qiongling Yu, Liang Jiang, Xiawei Hu, Qian Shi, Haiyan Gao, Hainv Li, Lanjuan |
| description | A novel avian-origin influenza A (H7N9) virus emerged and spread among humans in Eastern China in 2013. Prophylactic treatment with antibiotics and probiotics for secondary infection is as important as antiviral treatment. This study aims to assess the ability of probiotic treatment to restore internal homeostasis under antibiotic pressure and to reduce/ameliorate the risk of secondary infections resulting from infection with the H7N9 virus.
This is a retrospective study in archival samples. Between April 1 and May 10, 2013, 113 stool, sputum, and blood specimens were collected and analyzed by denaturing gradient gel electrophoresis (DGGE) to determine the composition of the patient microbiomes. Microbial diversity was calculated using Gel-Pro analyzer and Past software. Cluster analysis of DGGE pattern profiles was employed to create a phylogenetic tree for each patient, and multidimensional scaling (MDS) and principal component analysis (PCA) were performed to visualize relationships between individual lanes.
Five patients had secondary infections, including Klebsiella pneumonia, Acinetobacter baumanii and Candida albicans infection. The DGGE profiles of fecal samples obtained at different time points from the same individual were clearly different, particularly for patients with secondary infections. Shannon's diversity index and evenness index were lower in all infected groups compared to the control group. After B. subtilis and E. faecium or C. butyricum administration, the fecal bacterial profiles of patients who had not been treated with antibiotics displayed a trend of increasing diversity and evenness. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients, but administration of B. subtilis and E. faecium appeared to reduce/ameliorate secondary infection in one patient.
H7N9 infection might decrease intestinal microbial diversity and species richness in humans. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients. B. subtilis and E. faecium may also play a role in reducing/ameliorating secondary infection in these patients. |
| doi_str_mv | 10.1371/journal.pone.0151976 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1774172713</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A453470998</galeid><doaj_id>oai_doaj_org_article_33c2dba78e96437fba7d87d43241502a</doaj_id><sourcerecordid>A453470998</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-8756a2e30823a1bc1e8be280947b9d29da38f402e470fcae173c85c7cc8a7aa83</originalsourceid><addsrcrecordid>eNqNkltv1DAQhSMEoqXwDxBEQkLwsIsvSWy_IFVVoStVtIKlr9bEmex6lY0XO-H263HYtNqgPqA8ZDT55thzcpLkOSVzygV9t3G9b6GZ71yLc0JzqkTxIDmmirNZwQh_eFAfJU9C2BCSc1kUj5MjVigpKFXHyXK5xvS8rtF0qavTa-9K6zpr0qVH6LbYxnabXkNnYxnSRTuQWKU_bLdOuzh7IT6pod302P6G9Mb6PjxNHtXQBHw2vk-Srx_Ol2cXs8urj4uz08uZKRTrZlLkBTDkRDIOtDQUZYlMEpWJUlVMVcBlnRGGmSC1AaSCG5kbYYwEASD5SfJyr7trXNCjIUFTITIqmKA8Eos9UTnY6J23W_C_tAOr_zacX2nwcd0GNeeGVSUIiarIuKhjWUlRZZxlNCcMotb78bS-3GJloiEemono9Etr13rlvutMqFxQFgXejALefesxdHprg8GmgRZdv793zokSJKKv_kHv326kVhAXsG3t4rlmENWnUSnaptTg0vweKj4Vbq2J6alt7E8G3k4GItPhz24FfQh68eXz_7NXN1P29QG7Rmi6dXBN31nXhimY7UHjXQge6zuTKdFD-G_d0EP49Rj-OPbi8AfdDd2mnf8B0Lj9cg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1774172713</pqid></control><display><type>article</type><title>The Effect of Probiotic Treatment on Patients Infected with the H7N9 Influenza Virus</title><source>PLoS</source><source>MEDLINE</source><source>EZB Free E-Journals</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Hu, Xinjun ; Zhang, Hua ; Lu, Haifeng ; Qian, Guirong ; Lv, Longxian ; Zhang, Chunxia ; Guo, Jing ; Jiang, Haiyin ; Zheng, Beiwen ; Yang, Fengling ; Gu, Silan ; Chen, Yuanting ; Bao, Qiongling ; Yu, Liang ; Jiang, Xiawei ; Hu, Qian ; Shi, Haiyan ; Gao, Hainv ; Li, Lanjuan</creator><creatorcontrib>Hu, Xinjun ; Zhang, Hua ; Lu, Haifeng ; Qian, Guirong ; Lv, Longxian ; Zhang, Chunxia ; Guo, Jing ; Jiang, Haiyin ; Zheng, Beiwen ; Yang, Fengling ; Gu, Silan ; Chen, Yuanting ; Bao, Qiongling ; Yu, Liang ; Jiang, Xiawei ; Hu, Qian ; Shi, Haiyan ; Gao, Hainv ; Li, Lanjuan</creatorcontrib><description>A novel avian-origin influenza A (H7N9) virus emerged and spread among humans in Eastern China in 2013. Prophylactic treatment with antibiotics and probiotics for secondary infection is as important as antiviral treatment. This study aims to assess the ability of probiotic treatment to restore internal homeostasis under antibiotic pressure and to reduce/ameliorate the risk of secondary infections resulting from infection with the H7N9 virus.
This is a retrospective study in archival samples. Between April 1 and May 10, 2013, 113 stool, sputum, and blood specimens were collected and analyzed by denaturing gradient gel electrophoresis (DGGE) to determine the composition of the patient microbiomes. Microbial diversity was calculated using Gel-Pro analyzer and Past software. Cluster analysis of DGGE pattern profiles was employed to create a phylogenetic tree for each patient, and multidimensional scaling (MDS) and principal component analysis (PCA) were performed to visualize relationships between individual lanes.
Five patients had secondary infections, including Klebsiella pneumonia, Acinetobacter baumanii and Candida albicans infection. The DGGE profiles of fecal samples obtained at different time points from the same individual were clearly different, particularly for patients with secondary infections. Shannon's diversity index and evenness index were lower in all infected groups compared to the control group. After B. subtilis and E. faecium or C. butyricum administration, the fecal bacterial profiles of patients who had not been treated with antibiotics displayed a trend of increasing diversity and evenness. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients, but administration of B. subtilis and E. faecium appeared to reduce/ameliorate secondary infection in one patient.
H7N9 infection might decrease intestinal microbial diversity and species richness in humans. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients. B. subtilis and E. faecium may also play a role in reducing/ameliorating secondary infection in these patients.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0151976</identifier><identifier>PMID: 26987119</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotics ; Antiviral Agents - therapeutic use ; Avian flu ; Avian influenza ; Bacteria ; Biodiversity ; Biology and Life Sciences ; Cluster analysis ; Coinfection - drug therapy ; Coinfection - microbiology ; Collaboration ; Dosage and administration ; Drug therapy ; Ecology and Environmental Sciences ; Female ; Gel electrophoresis ; Genome, Viral ; Health risks ; Homeostasis ; Hospitals ; Humans ; Infections ; Infectious diseases ; Influenza ; Influenza A ; Influenza A Virus, H7N9 Subtype ; Influenza, Human - drug therapy ; Intestine ; Klebsiella ; Laboratories ; Male ; Medical diagnosis ; Medicine ; Medicine and Health Sciences ; Microbiomes ; Microbiota ; Microorganisms ; Multidimensional scaling ; Paths ; Patient outcomes ; Patients ; Phylogeny ; Pneumonia ; Principal components analysis ; Probiotics ; Probiotics - therapeutic use ; Research and Analysis Methods ; Retrospective Studies ; Risk factors ; Scaling ; Secondary infection ; Species diversity ; Species richness ; Sputum ; Treatment Outcome ; Trends ; Viruses</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0151976-e0151976</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Hu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Hu et al 2016 Hu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-8756a2e30823a1bc1e8be280947b9d29da38f402e470fcae173c85c7cc8a7aa83</citedby><cites>FETCH-LOGICAL-c692t-8756a2e30823a1bc1e8be280947b9d29da38f402e470fcae173c85c7cc8a7aa83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4795712/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4795712/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26987119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Xinjun</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><creatorcontrib>Lu, Haifeng</creatorcontrib><creatorcontrib>Qian, Guirong</creatorcontrib><creatorcontrib>Lv, Longxian</creatorcontrib><creatorcontrib>Zhang, Chunxia</creatorcontrib><creatorcontrib>Guo, Jing</creatorcontrib><creatorcontrib>Jiang, Haiyin</creatorcontrib><creatorcontrib>Zheng, Beiwen</creatorcontrib><creatorcontrib>Yang, Fengling</creatorcontrib><creatorcontrib>Gu, Silan</creatorcontrib><creatorcontrib>Chen, Yuanting</creatorcontrib><creatorcontrib>Bao, Qiongling</creatorcontrib><creatorcontrib>Yu, Liang</creatorcontrib><creatorcontrib>Jiang, Xiawei</creatorcontrib><creatorcontrib>Hu, Qian</creatorcontrib><creatorcontrib>Shi, Haiyan</creatorcontrib><creatorcontrib>Gao, Hainv</creatorcontrib><creatorcontrib>Li, Lanjuan</creatorcontrib><title>The Effect of Probiotic Treatment on Patients Infected with the H7N9 Influenza Virus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A novel avian-origin influenza A (H7N9) virus emerged and spread among humans in Eastern China in 2013. Prophylactic treatment with antibiotics and probiotics for secondary infection is as important as antiviral treatment. This study aims to assess the ability of probiotic treatment to restore internal homeostasis under antibiotic pressure and to reduce/ameliorate the risk of secondary infections resulting from infection with the H7N9 virus.
This is a retrospective study in archival samples. Between April 1 and May 10, 2013, 113 stool, sputum, and blood specimens were collected and analyzed by denaturing gradient gel electrophoresis (DGGE) to determine the composition of the patient microbiomes. Microbial diversity was calculated using Gel-Pro analyzer and Past software. Cluster analysis of DGGE pattern profiles was employed to create a phylogenetic tree for each patient, and multidimensional scaling (MDS) and principal component analysis (PCA) were performed to visualize relationships between individual lanes.
Five patients had secondary infections, including Klebsiella pneumonia, Acinetobacter baumanii and Candida albicans infection. The DGGE profiles of fecal samples obtained at different time points from the same individual were clearly different, particularly for patients with secondary infections. Shannon's diversity index and evenness index were lower in all infected groups compared to the control group. After B. subtilis and E. faecium or C. butyricum administration, the fecal bacterial profiles of patients who had not been treated with antibiotics displayed a trend of increasing diversity and evenness. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients, but administration of B. subtilis and E. faecium appeared to reduce/ameliorate secondary infection in one patient.
H7N9 infection might decrease intestinal microbial diversity and species richness in humans. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients. B. subtilis and E. faecium may also play a role in reducing/ameliorating secondary infection in these patients.</description><subject>Antibiotics</subject><subject>Antiviral Agents - therapeutic use</subject><subject>Avian flu</subject><subject>Avian influenza</subject><subject>Bacteria</subject><subject>Biodiversity</subject><subject>Biology and Life Sciences</subject><subject>Cluster analysis</subject><subject>Coinfection - drug therapy</subject><subject>Coinfection - microbiology</subject><subject>Collaboration</subject><subject>Dosage and administration</subject><subject>Drug therapy</subject><subject>Ecology and Environmental Sciences</subject><subject>Female</subject><subject>Gel electrophoresis</subject><subject>Genome, Viral</subject><subject>Health risks</subject><subject>Homeostasis</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza A Virus, H7N9 Subtype</subject><subject>Influenza, Human - drug therapy</subject><subject>Intestine</subject><subject>Klebsiella</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Microbiomes</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Multidimensional scaling</subject><subject>Paths</subject><subject>Patient outcomes</subject><subject>Patients</subject><subject>Phylogeny</subject><subject>Pneumonia</subject><subject>Principal components analysis</subject><subject>Probiotics</subject><subject>Probiotics - therapeutic use</subject><subject>Research and Analysis Methods</subject><subject>Retrospective Studies</subject><subject>Risk factors</subject><subject>Scaling</subject><subject>Secondary infection</subject><subject>Species diversity</subject><subject>Species richness</subject><subject>Sputum</subject><subject>Treatment Outcome</subject><subject>Trends</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkltv1DAQhSMEoqXwDxBEQkLwsIsvSWy_IFVVoStVtIKlr9bEmex6lY0XO-H263HYtNqgPqA8ZDT55thzcpLkOSVzygV9t3G9b6GZ71yLc0JzqkTxIDmmirNZwQh_eFAfJU9C2BCSc1kUj5MjVigpKFXHyXK5xvS8rtF0qavTa-9K6zpr0qVH6LbYxnabXkNnYxnSRTuQWKU_bLdOuzh7IT6pod302P6G9Mb6PjxNHtXQBHw2vk-Srx_Ol2cXs8urj4uz08uZKRTrZlLkBTDkRDIOtDQUZYlMEpWJUlVMVcBlnRGGmSC1AaSCG5kbYYwEASD5SfJyr7trXNCjIUFTITIqmKA8Eos9UTnY6J23W_C_tAOr_zacX2nwcd0GNeeGVSUIiarIuKhjWUlRZZxlNCcMotb78bS-3GJloiEemono9Etr13rlvutMqFxQFgXejALefesxdHprg8GmgRZdv793zokSJKKv_kHv326kVhAXsG3t4rlmENWnUSnaptTg0vweKj4Vbq2J6alt7E8G3k4GItPhz24FfQh68eXz_7NXN1P29QG7Rmi6dXBN31nXhimY7UHjXQge6zuTKdFD-G_d0EP49Rj-OPbi8AfdDd2mnf8B0Lj9cg</recordid><startdate>20160317</startdate><enddate>20160317</enddate><creator>Hu, Xinjun</creator><creator>Zhang, Hua</creator><creator>Lu, Haifeng</creator><creator>Qian, Guirong</creator><creator>Lv, Longxian</creator><creator>Zhang, Chunxia</creator><creator>Guo, Jing</creator><creator>Jiang, Haiyin</creator><creator>Zheng, Beiwen</creator><creator>Yang, Fengling</creator><creator>Gu, Silan</creator><creator>Chen, Yuanting</creator><creator>Bao, Qiongling</creator><creator>Yu, Liang</creator><creator>Jiang, Xiawei</creator><creator>Hu, Qian</creator><creator>Shi, Haiyan</creator><creator>Gao, Hainv</creator><creator>Li, Lanjuan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160317</creationdate><title>The Effect of Probiotic Treatment on Patients Infected with the H7N9 Influenza Virus</title><author>Hu, Xinjun ; Zhang, Hua ; Lu, Haifeng ; Qian, Guirong ; Lv, Longxian ; Zhang, Chunxia ; Guo, Jing ; Jiang, Haiyin ; Zheng, Beiwen ; Yang, Fengling ; Gu, Silan ; Chen, Yuanting ; Bao, Qiongling ; Yu, Liang ; Jiang, Xiawei ; Hu, Qian ; Shi, Haiyan ; Gao, Hainv ; Li, Lanjuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-8756a2e30823a1bc1e8be280947b9d29da38f402e470fcae173c85c7cc8a7aa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antibiotics</topic><topic>Antiviral Agents - therapeutic use</topic><topic>Avian flu</topic><topic>Avian influenza</topic><topic>Bacteria</topic><topic>Biodiversity</topic><topic>Biology and Life Sciences</topic><topic>Cluster analysis</topic><topic>Coinfection - drug therapy</topic><topic>Coinfection - microbiology</topic><topic>Collaboration</topic><topic>Dosage and administration</topic><topic>Drug therapy</topic><topic>Ecology and Environmental Sciences</topic><topic>Female</topic><topic>Gel electrophoresis</topic><topic>Genome, Viral</topic><topic>Health risks</topic><topic>Homeostasis</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>Influenza A Virus, H7N9 Subtype</topic><topic>Influenza, Human - drug therapy</topic><topic>Intestine</topic><topic>Klebsiella</topic><topic>Laboratories</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Microbiomes</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Multidimensional scaling</topic><topic>Paths</topic><topic>Patient outcomes</topic><topic>Patients</topic><topic>Phylogeny</topic><topic>Pneumonia</topic><topic>Principal components analysis</topic><topic>Probiotics</topic><topic>Probiotics - therapeutic use</topic><topic>Research and Analysis Methods</topic><topic>Retrospective Studies</topic><topic>Risk factors</topic><topic>Scaling</topic><topic>Secondary infection</topic><topic>Species diversity</topic><topic>Species richness</topic><topic>Sputum</topic><topic>Treatment Outcome</topic><topic>Trends</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Xinjun</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><creatorcontrib>Lu, Haifeng</creatorcontrib><creatorcontrib>Qian, Guirong</creatorcontrib><creatorcontrib>Lv, Longxian</creatorcontrib><creatorcontrib>Zhang, Chunxia</creatorcontrib><creatorcontrib>Guo, Jing</creatorcontrib><creatorcontrib>Jiang, Haiyin</creatorcontrib><creatorcontrib>Zheng, Beiwen</creatorcontrib><creatorcontrib>Yang, Fengling</creatorcontrib><creatorcontrib>Gu, Silan</creatorcontrib><creatorcontrib>Chen, Yuanting</creatorcontrib><creatorcontrib>Bao, Qiongling</creatorcontrib><creatorcontrib>Yu, Liang</creatorcontrib><creatorcontrib>Jiang, Xiawei</creatorcontrib><creatorcontrib>Hu, Qian</creatorcontrib><creatorcontrib>Shi, Haiyan</creatorcontrib><creatorcontrib>Gao, Hainv</creatorcontrib><creatorcontrib>Li, Lanjuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Xinjun</au><au>Zhang, Hua</au><au>Lu, Haifeng</au><au>Qian, Guirong</au><au>Lv, Longxian</au><au>Zhang, Chunxia</au><au>Guo, Jing</au><au>Jiang, Haiyin</au><au>Zheng, Beiwen</au><au>Yang, Fengling</au><au>Gu, Silan</au><au>Chen, Yuanting</au><au>Bao, Qiongling</au><au>Yu, Liang</au><au>Jiang, Xiawei</au><au>Hu, Qian</au><au>Shi, Haiyan</au><au>Gao, Hainv</au><au>Li, Lanjuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Probiotic Treatment on Patients Infected with the H7N9 Influenza Virus</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-17</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0151976</spage><epage>e0151976</epage><pages>e0151976-e0151976</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A novel avian-origin influenza A (H7N9) virus emerged and spread among humans in Eastern China in 2013. Prophylactic treatment with antibiotics and probiotics for secondary infection is as important as antiviral treatment. This study aims to assess the ability of probiotic treatment to restore internal homeostasis under antibiotic pressure and to reduce/ameliorate the risk of secondary infections resulting from infection with the H7N9 virus.
This is a retrospective study in archival samples. Between April 1 and May 10, 2013, 113 stool, sputum, and blood specimens were collected and analyzed by denaturing gradient gel electrophoresis (DGGE) to determine the composition of the patient microbiomes. Microbial diversity was calculated using Gel-Pro analyzer and Past software. Cluster analysis of DGGE pattern profiles was employed to create a phylogenetic tree for each patient, and multidimensional scaling (MDS) and principal component analysis (PCA) were performed to visualize relationships between individual lanes.
Five patients had secondary infections, including Klebsiella pneumonia, Acinetobacter baumanii and Candida albicans infection. The DGGE profiles of fecal samples obtained at different time points from the same individual were clearly different, particularly for patients with secondary infections. Shannon's diversity index and evenness index were lower in all infected groups compared to the control group. After B. subtilis and E. faecium or C. butyricum administration, the fecal bacterial profiles of patients who had not been treated with antibiotics displayed a trend of increasing diversity and evenness. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients, but administration of B. subtilis and E. faecium appeared to reduce/ameliorate secondary infection in one patient.
H7N9 infection might decrease intestinal microbial diversity and species richness in humans. C. butyricum failed to reduce/ameliorate secondary infection in H7N9-infected patients. B. subtilis and E. faecium may also play a role in reducing/ameliorating secondary infection in these patients.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26987119</pmid><doi>10.1371/journal.pone.0151976</doi><tpages>e0151976</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0151976-e0151976 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1774172713 |
| source | PLoS; MEDLINE; EZB Free E-Journals; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Antibiotics Antiviral Agents - therapeutic use Avian flu Avian influenza Bacteria Biodiversity Biology and Life Sciences Cluster analysis Coinfection - drug therapy Coinfection - microbiology Collaboration Dosage and administration Drug therapy Ecology and Environmental Sciences Female Gel electrophoresis Genome, Viral Health risks Homeostasis Hospitals Humans Infections Infectious diseases Influenza Influenza A Influenza A Virus, H7N9 Subtype Influenza, Human - drug therapy Intestine Klebsiella Laboratories Male Medical diagnosis Medicine Medicine and Health Sciences Microbiomes Microbiota Microorganisms Multidimensional scaling Paths Patient outcomes Patients Phylogeny Pneumonia Principal components analysis Probiotics Probiotics - therapeutic use Research and Analysis Methods Retrospective Studies Risk factors Scaling Secondary infection Species diversity Species richness Sputum Treatment Outcome Trends Viruses |
| title | The Effect of Probiotic Treatment on Patients Infected with the H7N9 Influenza Virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T13%3A36%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Effect%20of%20Probiotic%20Treatment%20on%20Patients%20Infected%20with%20the%20H7N9%20Influenza%20Virus&rft.jtitle=PloS%20one&rft.au=Hu,%20Xinjun&rft.date=2016-03-17&rft.volume=11&rft.issue=3&rft.spage=e0151976&rft.epage=e0151976&rft.pages=e0151976-e0151976&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0151976&rft_dat=%3Cgale_plos_%3EA453470998%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1774172713&rft_id=info:pmid/26987119&rft_galeid=A453470998&rft_doaj_id=oai_doaj_org_article_33c2dba78e96437fba7d87d43241502a&rfr_iscdi=true |