Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells
Transplantation of ex vivo expanded corneal limbal stem cells (LSCs) has been the main treatment for limbal stem cell deficiency, although the shortage of donor corneal tissues remains a major concern for its wide application. Due to the development of tissue engineering, embryonic stem cells (ESCs)...
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| creator | Wang, Zhenyu Zhou, Qingjun Duan, Haoyun Wang, Yao Dong, Muchen Shi, Weiyun |
| description | Transplantation of ex vivo expanded corneal limbal stem cells (LSCs) has been the main treatment for limbal stem cell deficiency, although the shortage of donor corneal tissues remains a major concern for its wide application. Due to the development of tissue engineering, embryonic stem cells (ESCs)-derived corneal epithelial-like cells (ESC-CECs) become a new direction for this issue. However, the immunogenicity of ESC-CECs is a critical matter to be solved. In the present study, we explored the immunological properties of ESC-CECs, which were differentiated from ESCs. The results showed that ESC-CECs had a similar character and function with LSCs both in vitro and in vivo. In ESC-CECs, a large number of genes related with immune response were down-regulated. The expressions of MHC-I, MHC-II, and co-stimulatory molecules were low, but the expression of HLA-G was high. The ESC-CECs were less responsible for T cell proliferation and NK cell lysis in vitro, and there was less immune cell infiltration after transplantation in vivo compared with LSCs. Moreover, the immunological properties were not affected by interferon-γ. All these results indicated a low immunogenicity of ESC-CECs, and they can be promising in clinical use. |
| doi_str_mv | 10.1371/journal.pone.0150731 |
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Due to the development of tissue engineering, embryonic stem cells (ESCs)-derived corneal epithelial-like cells (ESC-CECs) become a new direction for this issue. However, the immunogenicity of ESC-CECs is a critical matter to be solved. In the present study, we explored the immunological properties of ESC-CECs, which were differentiated from ESCs. The results showed that ESC-CECs had a similar character and function with LSCs both in vitro and in vivo. In ESC-CECs, a large number of genes related with immune response were down-regulated. The expressions of MHC-I, MHC-II, and co-stimulatory molecules were low, but the expression of HLA-G was high. The ESC-CECs were less responsible for T cell proliferation and NK cell lysis in vitro, and there was less immune cell infiltration after transplantation in vivo compared with LSCs. Moreover, the immunological properties were not affected by interferon-γ. All these results indicated a low immunogenicity of ESC-CECs, and they can be promising in clinical use.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0150731</identifier><identifier>PMID: 26977925</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antigens ; Biology and Life Sciences ; Cell Proliferation ; Cornea ; Embryo cells ; Embryogenesis ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - immunology ; Embryonic Stem Cells - metabolism ; Embryos ; Ethics ; Gene Expression ; Genomes ; Histocompatibility antigen HLA ; Humans ; Immune response ; Immune system ; Immunogenicity ; Immunologic research ; Immunology ; Infiltration ; Interferon ; Laboratories ; Lymphocytes T ; Lysis ; Major histocompatibility complex ; Male ; Medicine and Health Sciences ; Natural killer cells ; Physiological aspects ; Properties (attributes) ; Rabbits ; Stem cell research ; Stem Cell Transplantation ; Stem cells ; Studies ; T-Lymphocytes - cytology ; Tissue engineering ; Transplantation</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0150731-e0150731</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Wang et al 2016 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-194b6c699957602c34c01200a70ec21431c7e9f5cac6818f3946369104432873</citedby><cites>FETCH-LOGICAL-c692t-194b6c699957602c34c01200a70ec21431c7e9f5cac6818f3946369104432873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792422/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792422/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79472,79473</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26977925$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Fraidenraich, Diego</contributor><creatorcontrib>Wang, Zhenyu</creatorcontrib><creatorcontrib>Zhou, Qingjun</creatorcontrib><creatorcontrib>Duan, Haoyun</creatorcontrib><creatorcontrib>Wang, Yao</creatorcontrib><creatorcontrib>Dong, Muchen</creatorcontrib><creatorcontrib>Shi, Weiyun</creatorcontrib><title>Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Transplantation of ex vivo expanded corneal limbal stem cells (LSCs) has been the main treatment for limbal stem cell deficiency, although the shortage of donor corneal tissues remains a major concern for its wide application. Due to the development of tissue engineering, embryonic stem cells (ESCs)-derived corneal epithelial-like cells (ESC-CECs) become a new direction for this issue. However, the immunogenicity of ESC-CECs is a critical matter to be solved. In the present study, we explored the immunological properties of ESC-CECs, which were differentiated from ESCs. The results showed that ESC-CECs had a similar character and function with LSCs both in vitro and in vivo. In ESC-CECs, a large number of genes related with immune response were down-regulated. The expressions of MHC-I, MHC-II, and co-stimulatory molecules were low, but the expression of HLA-G was high. The ESC-CECs were less responsible for T cell proliferation and NK cell lysis in vitro, and there was less immune cell infiltration after transplantation in vivo compared with LSCs. Moreover, the immunological properties were not affected by interferon-γ. All these results indicated a low immunogenicity of ESC-CECs, and they can be promising in clinical use.</description><subject>Animals</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Cell Proliferation</subject><subject>Cornea</subject><subject>Embryo cells</subject><subject>Embryogenesis</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - immunology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Embryos</subject><subject>Ethics</subject><subject>Gene Expression</subject><subject>Genomes</subject><subject>Histocompatibility antigen HLA</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunogenicity</subject><subject>Immunologic research</subject><subject>Immunology</subject><subject>Infiltration</subject><subject>Interferon</subject><subject>Laboratories</subject><subject>Lymphocytes T</subject><subject>Lysis</subject><subject>Major histocompatibility complex</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Natural killer cells</subject><subject>Physiological aspects</subject><subject>Properties (attributes)</subject><subject>Rabbits</subject><subject>Stem cell research</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Studies</subject><subject>T-Lymphocytes - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhenyu</au><au>Zhou, Qingjun</au><au>Duan, Haoyun</au><au>Wang, Yao</au><au>Dong, Muchen</au><au>Shi, Weiyun</au><au>Fraidenraich, Diego</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-15</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0150731</spage><epage>e0150731</epage><pages>e0150731-e0150731</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Transplantation of ex vivo expanded corneal limbal stem cells (LSCs) has been the main treatment for limbal stem cell deficiency, although the shortage of donor corneal tissues remains a major concern for its wide application. Due to the development of tissue engineering, embryonic stem cells (ESCs)-derived corneal epithelial-like cells (ESC-CECs) become a new direction for this issue. However, the immunogenicity of ESC-CECs is a critical matter to be solved. In the present study, we explored the immunological properties of ESC-CECs, which were differentiated from ESCs. The results showed that ESC-CECs had a similar character and function with LSCs both in vitro and in vivo. In ESC-CECs, a large number of genes related with immune response were down-regulated. The expressions of MHC-I, MHC-II, and co-stimulatory molecules were low, but the expression of HLA-G was high. The ESC-CECs were less responsible for T cell proliferation and NK cell lysis in vitro, and there was less immune cell infiltration after transplantation in vivo compared with LSCs. Moreover, the immunological properties were not affected by interferon-γ. All these results indicated a low immunogenicity of ESC-CECs, and they can be promising in clinical use.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26977925</pmid><doi>10.1371/journal.pone.0150731</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens Biology and Life Sciences Cell Proliferation Cornea Embryo cells Embryogenesis Embryonic Stem Cells - cytology Embryonic Stem Cells - immunology Embryonic Stem Cells - metabolism Embryos Ethics Gene Expression Genomes Histocompatibility antigen HLA Humans Immune response Immune system Immunogenicity Immunologic research Immunology Infiltration Interferon Laboratories Lymphocytes T Lysis Major histocompatibility complex Male Medicine and Health Sciences Natural killer cells Physiological aspects Properties (attributes) Rabbits Stem cell research Stem Cell Transplantation Stem cells Studies T-Lymphocytes - cytology Tissue engineering Transplantation |
| title | Immunological Properties of Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells |
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