Identification of the Genome Segments of Bluetongue Virus Serotype 26 (Isolate KUW2010/02) that Restrict Replication in a Culicoides sonorensis Cell Line (KC Cells)
Bluetongue virus (BTV) can infect most ruminant species and is usually transmitted by adult, vector-competent biting midges (Culicoides spp.). Infection with BTV can cause severe clinical signs and can be fatal, particularly in naïve sheep and some deer species. Although 24 distinct BTV serotypes we...
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| creator | Pullinger, Gillian D Guimerà Busquets, Marc Nomikou, Kyriaki Boyce, Mark Attoui, Houssam Mertens, Peter P |
| description | Bluetongue virus (BTV) can infect most ruminant species and is usually transmitted by adult, vector-competent biting midges (Culicoides spp.). Infection with BTV can cause severe clinical signs and can be fatal, particularly in naïve sheep and some deer species. Although 24 distinct BTV serotypes were recognized for several decades, additional 'types' have recently been identified, including BTV-25 (from Switzerland), BTV-26 (from Kuwait) and BTV-27 from France (Corsica). Although BTV-25 has failed to grow in either insect or mammalian cell cultures, BTV-26 (isolate KUW2010/02), which can be transmitted horizontally between goats in the absence of vector insects, does not replicate in a Culicoides sonorensis cell line (KC cells) but can be propagated in mammalian cells (BSR cells). The BTV genome consists of ten segments of linear dsRNA. Mono-reassortant viruses were generated by reverse-genetics, each one containing a single BTV-26 genome segment in a BTV-1 genetic-background. However, attempts to recover a mono-reassortant containing genome-segment 2 (Seg-2) of BTV-26 (encoding VP2), were unsuccessful but a triple-reassortant was successfully generated containing Seg-2, Seg-6 and Seg-7 (encoding VP5 and VP7 respectively) of BTV-26. Reassortants were recovered and most replicated well in mammalian cells (BSR cells). However, mono-reassortants containing Seg-1 or Seg-3 of BTV-26 (encoding VP1, or VP3 respectively) and the triple reassortant failed to replicate, while a mono-reassortant containing Seg-7 of BTV-26 only replicated slowly in KC cells. |
| doi_str_mv | 10.1371/journal.pone.0149709 |
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Infection with BTV can cause severe clinical signs and can be fatal, particularly in naïve sheep and some deer species. Although 24 distinct BTV serotypes were recognized for several decades, additional 'types' have recently been identified, including BTV-25 (from Switzerland), BTV-26 (from Kuwait) and BTV-27 from France (Corsica). Although BTV-25 has failed to grow in either insect or mammalian cell cultures, BTV-26 (isolate KUW2010/02), which can be transmitted horizontally between goats in the absence of vector insects, does not replicate in a Culicoides sonorensis cell line (KC cells) but can be propagated in mammalian cells (BSR cells). The BTV genome consists of ten segments of linear dsRNA. Mono-reassortant viruses were generated by reverse-genetics, each one containing a single BTV-26 genome segment in a BTV-1 genetic-background. However, attempts to recover a mono-reassortant containing genome-segment 2 (Seg-2) of BTV-26 (encoding VP2), were unsuccessful but a triple-reassortant was successfully generated containing Seg-2, Seg-6 and Seg-7 (encoding VP5 and VP7 respectively) of BTV-26. Reassortants were recovered and most replicated well in mammalian cells (BSR cells). However, mono-reassortants containing Seg-1 or Seg-3 of BTV-26 (encoding VP1, or VP3 respectively) and the triple reassortant failed to replicate, while a mono-reassortant containing Seg-7 of BTV-26 only replicated slowly in KC cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0149709</identifier><identifier>PMID: 26890863</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Antigens ; Arthropods ; Base Sequence ; Biology and life sciences ; Biting ; Bluetongue ; Bluetongue virus - genetics ; Bluetongue virus - growth & development ; Cell Line ; Cells (Biology) ; Ceratopogonidae - virology ; Clone Cells ; Culicoides ; Deer ; Disease ; Double-stranded RNA ; Genetics ; Genome, Viral ; Genomes ; Goats ; Infection ; Insects ; Kinetics ; Life Sciences ; Mammalian cells ; Mammals ; Medicine and Health Sciences ; Phylogenetics ; Plasmids - metabolism ; Proteins ; Reassortant Viruses - genetics ; Research and Analysis Methods ; Reverse Genetics ; RNA polymerase ; RNA, Viral - genetics ; Segments ; Serogroup ; Serotypes ; Sheep ; Vectors (Biology) ; Virology ; Virus Replication - genetics ; Viruses ; VP1 protein ; VP3 protein</subject><ispartof>PloS one, 2016-02, Vol.11 (2), p.e0149709-e0149709</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Pullinger et al. 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pullinger, Gillian D</au><au>Guimerà Busquets, Marc</au><au>Nomikou, Kyriaki</au><au>Boyce, Mark</au><au>Attoui, Houssam</au><au>Mertens, Peter P</au><au>Andrei, Graciela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the Genome Segments of Bluetongue Virus Serotype 26 (Isolate KUW2010/02) that Restrict Replication in a Culicoides sonorensis Cell Line (KC Cells)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-02-18</date><risdate>2016</risdate><volume>11</volume><issue>2</issue><spage>e0149709</spage><epage>e0149709</epage><pages>e0149709-e0149709</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bluetongue virus (BTV) can infect most ruminant species and is usually transmitted by adult, vector-competent biting midges (Culicoides spp.). Infection with BTV can cause severe clinical signs and can be fatal, particularly in naïve sheep and some deer species. Although 24 distinct BTV serotypes were recognized for several decades, additional 'types' have recently been identified, including BTV-25 (from Switzerland), BTV-26 (from Kuwait) and BTV-27 from France (Corsica). Although BTV-25 has failed to grow in either insect or mammalian cell cultures, BTV-26 (isolate KUW2010/02), which can be transmitted horizontally between goats in the absence of vector insects, does not replicate in a Culicoides sonorensis cell line (KC cells) but can be propagated in mammalian cells (BSR cells). The BTV genome consists of ten segments of linear dsRNA. Mono-reassortant viruses were generated by reverse-genetics, each one containing a single BTV-26 genome segment in a BTV-1 genetic-background. However, attempts to recover a mono-reassortant containing genome-segment 2 (Seg-2) of BTV-26 (encoding VP2), were unsuccessful but a triple-reassortant was successfully generated containing Seg-2, Seg-6 and Seg-7 (encoding VP5 and VP7 respectively) of BTV-26. Reassortants were recovered and most replicated well in mammalian cells (BSR cells). However, mono-reassortants containing Seg-1 or Seg-3 of BTV-26 (encoding VP1, or VP3 respectively) and the triple reassortant failed to replicate, while a mono-reassortant containing Seg-7 of BTV-26 only replicated slowly in KC cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26890863</pmid><doi>10.1371/journal.pone.0149709</doi><orcidid>https://orcid.org/0000-0001-9639-9981</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1771272126 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Antigens Arthropods Base Sequence Biology and life sciences Biting Bluetongue Bluetongue virus - genetics Bluetongue virus - growth & development Cell Line Cells (Biology) Ceratopogonidae - virology Clone Cells Culicoides Deer Disease Double-stranded RNA Genetics Genome, Viral Genomes Goats Infection Insects Kinetics Life Sciences Mammalian cells Mammals Medicine and Health Sciences Phylogenetics Plasmids - metabolism Proteins Reassortant Viruses - genetics Research and Analysis Methods Reverse Genetics RNA polymerase RNA, Viral - genetics Segments Serogroup Serotypes Sheep Vectors (Biology) Virology Virus Replication - genetics Viruses VP1 protein VP3 protein |
| title | Identification of the Genome Segments of Bluetongue Virus Serotype 26 (Isolate KUW2010/02) that Restrict Replication in a Culicoides sonorensis Cell Line (KC Cells) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T23%3A29%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20the%20Genome%20Segments%20of%20Bluetongue%20Virus%20Serotype%2026%20(Isolate%20KUW2010/02)%20that%20Restrict%20Replication%20in%20a%20Culicoides%20sonorensis%20Cell%20Line%20(KC%20Cells)&rft.jtitle=PloS%20one&rft.au=Pullinger,%20Gillian%20D&rft.date=2016-02-18&rft.volume=11&rft.issue=2&rft.spage=e0149709&rft.epage=e0149709&rft.pages=e0149709-e0149709&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0149709&rft_dat=%3Cgale_plos_%3EA443614609%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1771272126&rft_id=info:pmid/26890863&rft_galeid=A443614609&rft_doaj_id=oai_doaj_org_article_ba4ba71d361c4500a862aeb754997b2d&rfr_iscdi=true |