Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma
The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reacti...
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| Veröffentlicht in: | PloS one 2016-02, Vol.11 (2), p.e0150279-e0150279 |
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| creator | Lee, Jung-Hwan Om, Ji-Yeon Kim, Yong-Hee Kim, Kwang-Mahn Choi, Eun-Ha Kim, Kyoung-Nam |
| description | The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals. |
| doi_str_mv | 10.1371/journal.pone.0150279 |
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CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0150279</identifier><identifier>PMID: 26919318</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetylcysteine - pharmacology ; Analysis ; Antioxidants ; Apoptosis ; Atmospheric pressure ; Bioengineering ; Biology and Life Sciences ; Biomedical materials ; Bone marrow ; Brain research ; Breast cancer ; Cancer therapies ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - therapy ; Care and treatment ; Cell culture ; Cell cycle ; Cells, Cultured ; Cold ; Cold effects ; Cold pressing ; Culture media ; Culture Media - pharmacology ; Cytotoxicity ; Degradation ; Dentistry ; Deoxyribonucleic acid ; DNA ; Drug Screening Assays, Antitumor ; Epidermal growth factor ; Epidermal growth factor receptors ; ErbB Receptors - antagonists & inhibitors ; Fibroblasts - drug effects ; Free Radicals ; Genetic aspects ; Gingiva - cytology ; Health aspects ; Humans ; Kinases ; Medicine and Health Sciences ; Metabolism ; Mouth Neoplasms - metabolism ; Mouth Neoplasms - pathology ; Mouth Neoplasms - therapy ; Neoplasm Proteins - antagonists & inhibitors ; Nitric oxide ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - pharmacology ; Oral cancer ; Oral squamous cell carcinoma ; Oxidative Stress ; Physical Sciences ; Plasma ; Plasma Gases - pharmacology ; Plasma Gases - therapeutic use ; Plasmas (physics) ; Proteolysis ; Radicals ; Reactive Nitrogen Species - pharmacology ; Reactive Oxygen Species ; Research and Analysis Methods ; Selective media ; Squamous cell carcinoma ; Studies ; Sulfhydryl Compounds - analysis ; Tumor Cells, Cultured</subject><ispartof>PloS one, 2016-02, Vol.11 (2), p.e0150279-e0150279</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Lee et al 2016 Lee et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-f74497374c12ca2295149ebbcc56a47a97870c93e408824c8a35fc190a7269243</citedby><cites>FETCH-LOGICAL-c692t-f74497374c12ca2295149ebbcc56a47a97870c93e408824c8a35fc190a7269243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768860/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768860/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26919318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yousfi, Mohammed</contributor><creatorcontrib>Lee, Jung-Hwan</creatorcontrib><creatorcontrib>Om, Ji-Yeon</creatorcontrib><creatorcontrib>Kim, Yong-Hee</creatorcontrib><creatorcontrib>Kim, Kwang-Mahn</creatorcontrib><creatorcontrib>Choi, Eun-Ha</creatorcontrib><creatorcontrib>Kim, Kyoung-Nam</creatorcontrib><title>Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.</description><subject>Acetylcysteine - pharmacology</subject><subject>Analysis</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Atmospheric pressure</subject><subject>Bioengineering</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Bone marrow</subject><subject>Brain research</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - therapy</subject><subject>Care and treatment</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cells, Cultured</subject><subject>Cold</subject><subject>Cold effects</subject><subject>Cold pressing</subject><subject>Culture media</subject><subject>Culture Media - pharmacology</subject><subject>Cytotoxicity</subject><subject>Degradation</subject><subject>Dentistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Epidermal growth factor</subject><subject>Epidermal growth factor receptors</subject><subject>ErbB Receptors - antagonists & inhibitors</subject><subject>Fibroblasts - drug effects</subject><subject>Free Radicals</subject><subject>Genetic aspects</subject><subject>Gingiva - cytology</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Kinases</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Mouth Neoplasms - pathology</subject><subject>Mouth Neoplasms - therapy</subject><subject>Neoplasm Proteins - antagonists & inhibitors</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - pharmacology</subject><subject>Oral cancer</subject><subject>Oral squamous cell carcinoma</subject><subject>Oxidative Stress</subject><subject>Physical Sciences</subject><subject>Plasma</subject><subject>Plasma Gases - pharmacology</subject><subject>Plasma Gases - therapeutic use</subject><subject>Plasmas (physics)</subject><subject>Proteolysis</subject><subject>Radicals</subject><subject>Reactive Nitrogen Species - pharmacology</subject><subject>Reactive Oxygen Species</subject><subject>Research and Analysis Methods</subject><subject>Selective media</subject><subject>Squamous cell carcinoma</subject><subject>Studies</subject><subject>Sulfhydryl Compounds - analysis</subject><subject>Tumor Cells, Cultured</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggsISG4aPG_2vHNpKlso2Ki0wbcWq5z0npy4s5ONvYwvCsu7aYW7QLlItY5v-9z_MWnKF4TPCRMkk9XoY-t8cNlaGGIyQhTqZ4U-0QxOhAUs6db673iRUpXGI9YKcTzYo8KlXuk3C9-X4IH27kbQF-d966do-O6zpWEQo3GwVfoqGtCWi4gOovOI6TUR0Dn3qTGoFvXLdC3KZq0VW-hQp_vUt232S-0K_3x0lUQG-PRaQy3GT0xtgsRXYCF5WrhWjSNuX153Zsm9AmNwXs0NtG6NjTmZfGsNj7Bq837oPhxcvx9_GVwNj2djI_OBlYo2g1qybmSTHJLqDWUqhHhCmYza0fCcGmULCW2igHHZUm5LQ0b1ZYobGROgnJ2ULxd-y59SHqTbNJESkIlEUJkYrImqmCu9DK6xsQ7HYzTfwshzrWJnbMeNJ9hQYAoYqqagxXGcIpVRaSgTOC6yl6Hm936WQOVhbbLGeyY7nZat9DzcKO5FGUpcDb4sDGI4bqH1OnGJZuTMy3kEPN3CyWo5FRm9N0_6OOn21Bzkw_g2jrkfe3KVB9xzplgCrNMDR-h8lNB42y-hrXL9R3Bxx1BZjr41c1Nn5KeXF78Pzv9ucu-32IXYHy3SMH3q2uXdkG-Bm0MKUWoH0ImWK-m6D4NvZoivZmiLHuz_YMeRPdjw_4A65kXmQ</recordid><startdate>20160226</startdate><enddate>20160226</enddate><creator>Lee, Jung-Hwan</creator><creator>Om, Ji-Yeon</creator><creator>Kim, Yong-Hee</creator><creator>Kim, Kwang-Mahn</creator><creator>Choi, Eun-Ha</creator><creator>Kim, Kyoung-Nam</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160226</creationdate><title>Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma</title><author>Lee, Jung-Hwan ; Om, Ji-Yeon ; Kim, Yong-Hee ; Kim, Kwang-Mahn ; Choi, Eun-Ha ; Kim, Kyoung-Nam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-f74497374c12ca2295149ebbcc56a47a97870c93e408824c8a35fc190a7269243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acetylcysteine - pharmacology</topic><topic>Analysis</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Atmospheric pressure</topic><topic>Bioengineering</topic><topic>Biology and Life Sciences</topic><topic>Biomedical materials</topic><topic>Bone marrow</topic><topic>Brain research</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - therapy</topic><topic>Care and treatment</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cells, Cultured</topic><topic>Cold</topic><topic>Cold effects</topic><topic>Cold pressing</topic><topic>Culture media</topic><topic>Culture Media - pharmacology</topic><topic>Cytotoxicity</topic><topic>Degradation</topic><topic>Dentistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Epidermal growth factor</topic><topic>Epidermal growth factor receptors</topic><topic>ErbB Receptors - antagonists & inhibitors</topic><topic>Fibroblasts - drug effects</topic><topic>Free Radicals</topic><topic>Genetic aspects</topic><topic>Gingiva - cytology</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Kinases</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Mouth Neoplasms - pathology</topic><topic>Mouth Neoplasms - therapy</topic><topic>Neoplasm Proteins - antagonists & inhibitors</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - pharmacology</topic><topic>Oral cancer</topic><topic>Oral squamous cell carcinoma</topic><topic>Oxidative Stress</topic><topic>Physical Sciences</topic><topic>Plasma</topic><topic>Plasma Gases - pharmacology</topic><topic>Plasma Gases - therapeutic use</topic><topic>Plasmas (physics)</topic><topic>Proteolysis</topic><topic>Radicals</topic><topic>Reactive Nitrogen Species - pharmacology</topic><topic>Reactive Oxygen Species</topic><topic>Research and Analysis Methods</topic><topic>Selective media</topic><topic>Squamous cell carcinoma</topic><topic>Studies</topic><topic>Sulfhydryl Compounds - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Jung-Hwan</au><au>Om, Ji-Yeon</au><au>Kim, Yong-Hee</au><au>Kim, Kwang-Mahn</au><au>Choi, Eun-Ha</au><au>Kim, Kyoung-Nam</au><au>Yousfi, Mohammed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-02-26</date><risdate>2016</risdate><volume>11</volume><issue>2</issue><spage>e0150279</spage><epage>e0150279</epage><pages>e0150279-e0150279</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26919318</pmid><doi>10.1371/journal.pone.0150279</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2016-02, Vol.11 (2), p.e0150279-e0150279 |
| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acetylcysteine - pharmacology Analysis Antioxidants Apoptosis Atmospheric pressure Bioengineering Biology and Life Sciences Biomedical materials Bone marrow Brain research Breast cancer Cancer therapies Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - therapy Care and treatment Cell culture Cell cycle Cells, Cultured Cold Cold effects Cold pressing Culture media Culture Media - pharmacology Cytotoxicity Degradation Dentistry Deoxyribonucleic acid DNA Drug Screening Assays, Antitumor Epidermal growth factor Epidermal growth factor receptors ErbB Receptors - antagonists & inhibitors Fibroblasts - drug effects Free Radicals Genetic aspects Gingiva - cytology Health aspects Humans Kinases Medicine and Health Sciences Metabolism Mouth Neoplasms - metabolism Mouth Neoplasms - pathology Mouth Neoplasms - therapy Neoplasm Proteins - antagonists & inhibitors Nitric oxide Nitric Oxide - antagonists & inhibitors Nitric Oxide - pharmacology Oral cancer Oral squamous cell carcinoma Oxidative Stress Physical Sciences Plasma Plasma Gases - pharmacology Plasma Gases - therapeutic use Plasmas (physics) Proteolysis Radicals Reactive Nitrogen Species - pharmacology Reactive Oxygen Species Research and Analysis Methods Selective media Squamous cell carcinoma Studies Sulfhydryl Compounds - analysis Tumor Cells, Cultured |
| title | Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T11%3A40%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Selective%20Killing%20Effects%20of%20Cold%20Atmospheric%20Pressure%20Plasma%20with%20NO%20Induced%20Dysfunction%20of%20Epidermal%20Growth%20Factor%20Receptor%20in%20Oral%20Squamous%20Cell%20Carcinoma&rft.jtitle=PloS%20one&rft.au=Lee,%20Jung-Hwan&rft.date=2016-02-26&rft.volume=11&rft.issue=2&rft.spage=e0150279&rft.epage=e0150279&rft.pages=e0150279-e0150279&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0150279&rft_dat=%3Cgale_plos_%3EA444363903%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1771271666&rft_id=info:pmid/26919318&rft_galeid=A444363903&rft_doaj_id=oai_doaj_org_article_4b061e191adf4ec6aa4209d1762360fd&rfr_iscdi=true |