Optimised Pre-Analytical Methods Improve KRAS Mutation Detection in Circulating Tumour DNA (ctDNA) from Patients with Non-Small Cell Lung Cancer (NSCLC)

Non-invasive mutation testing using circulating tumour DNA (ctDNA) is an attractive premise. This could enable patients without available tumour sample to access more treatment options. Peripheral blood and matched tumours were analysed from 45 NSCLC patients. We investigated the impact of pre-analy...

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Veröffentlicht in:	PloS one 2016-02, Vol.11 (2), p.e0150197-e0150197
Hauptverfasser:	Sherwood, James L, Corcoran, Claire, Brown, Helen, Sharpe, Alan D, Musilova, Milena, Kohlmann, Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma - blood Adenocarcinoma - chemistry Adenocarcinoma - genetics Analytical methods Automation Biology and Life Sciences Biomarkers Blood Blood Specimen Collection - instrumentation Blood Specimen Collection - methods Carcinoma, Non-Small-Cell Lung - blood Carcinoma, Non-Small-Cell Lung - chemistry Carcinoma, Non-Small-Cell Lung - genetics Centrifugation Centrifugation - methods Collection Consent Contamination Deoxyribonucleic acid DNA DNA Mutational Analysis - methods DNA, Neoplasm - blood DNA, Neoplasm - genetics DNA, Neoplasm - isolation & purification Ethics Ethylenediaminetetraacetic acids Extraction Gene amplification Genes, ras Humans K-Ras protein Kinases Lung cancer Lung diseases Lung Neoplasms - blood Lung Neoplasms - chemistry Lung Neoplasms - genetics Mathematical analysis Medical research Medicine and Health Sciences Mutation Neoplasm Proteins - genetics Non-small cell lung carcinoma Patients Peripheral blood Plasma Plasmas (physics) Proto-Oncogene Proteins p21(ras) - genetics Reagent Kits, Diagnostic Research and analysis methods Science Solutions Specimen Handling - methods Studies Temperature Time Factors Tubes Tumors
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