Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear
Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic...
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| creator | Aliuos, Pooyan Schulze, Jennifer Schomaker, Markus Reuter, Günter Stolle, Stefan R O Werner, Darja Ripken, Tammo Lenarz, Thomas Warnecke, Athanasia |
| description | Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated.
Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB.
Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release.
Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest. |
| doi_str_mv | 10.1371/journal.pone.0150057 |
| format | Article |
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Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB.
Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release.
Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0150057</identifier><identifier>PMID: 26918945</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adhesion ; Animals ; Atomic force microscopy ; Beads ; Biology and Life Sciences ; Biomedical materials ; Brain research ; Brain-derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - administration & dosage ; Brain-Derived Neurotrophic Factor - genetics ; Cell Survival - drug effects ; Coating effects ; Cochlea ; Cochlear Implants ; Cultivation ; Cytotoxicity ; Data processing ; Drug delivery ; Drug Delivery Systems ; Drug Implants ; Ear, Inner - drug effects ; Electrodes ; Electromagnetic fields ; Emulsion polymerization ; Fibroblasts ; Genetic modification ; Hair ; Hearing impairment ; Hearing loss ; Inner ear ; Internalization ; Labeling ; Labelling ; Magnetics ; Medical schools ; Medicine and Health Sciences ; Mice ; Microscopy ; Neurons ; Neuroprotection ; NIH 3T3 Cells ; Otolaryngology ; Physical Sciences ; Physiological aspects ; Research and Analysis Methods ; Signal transduction ; Studies ; Surgery ; Surgical implants ; Transplants & implants</subject><ispartof>PloS one, 2016-02, Vol.11 (2), p.e0150057-e0150057</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Aliuos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Aliuos et al 2016 Aliuos et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-eb2a37b0d110d7c5b316cc2dc29c2a73d277c5ce75c6e8b2e6be7dab00d34c173</citedby><cites>FETCH-LOGICAL-c692t-eb2a37b0d110d7c5b316cc2dc29c2a73d277c5ce75c6e8b2e6be7dab00d34c173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4769079/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4769079/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26918945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Allodi, Silvana</contributor><creatorcontrib>Aliuos, Pooyan</creatorcontrib><creatorcontrib>Schulze, Jennifer</creatorcontrib><creatorcontrib>Schomaker, Markus</creatorcontrib><creatorcontrib>Reuter, Günter</creatorcontrib><creatorcontrib>Stolle, Stefan R O</creatorcontrib><creatorcontrib>Werner, Darja</creatorcontrib><creatorcontrib>Ripken, Tammo</creatorcontrib><creatorcontrib>Lenarz, Thomas</creatorcontrib><creatorcontrib>Warnecke, Athanasia</creatorcontrib><title>Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated.
Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB.
Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release.
Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Atomic force microscopy</subject><subject>Beads</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Brain research</subject><subject>Brain-derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - administration & dosage</subject><subject>Brain-Derived Neurotrophic Factor - genetics</subject><subject>Cell Survival - drug effects</subject><subject>Coating effects</subject><subject>Cochlea</subject><subject>Cochlear Implants</subject><subject>Cultivation</subject><subject>Cytotoxicity</subject><subject>Data processing</subject><subject>Drug delivery</subject><subject>Drug Delivery Systems</subject><subject>Drug Implants</subject><subject>Ear, Inner - drug effects</subject><subject>Electrodes</subject><subject>Electromagnetic fields</subject><subject>Emulsion polymerization</subject><subject>Fibroblasts</subject><subject>Genetic modification</subject><subject>Hair</subject><subject>Hearing impairment</subject><subject>Hearing loss</subject><subject>Inner ear</subject><subject>Internalization</subject><subject>Labeling</subject><subject>Labelling</subject><subject>Magnetics</subject><subject>Medical schools</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Neurons</subject><subject>Neuroprotection</subject><subject>NIH 3T3 Cells</subject><subject>Otolaryngology</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Research and Analysis Methods</subject><subject>Signal transduction</subject><subject>Studies</subject><subject>Surgery</subject><subject>Surgical implants</subject><subject>Transplants & implants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11vFCEUhidGY2v1HxglMTF6MSsfM7DTC5O1H3aT1ja29pYwcGaXZhZWYBr7U_y3snbbdE0vDCSQw3Ne4IVTFK8JHhEmyKcrPwSn-tHSOxhhUmNciyfFNmkYLTnF7OmD-VbxIsarTLAx58-LLcobMm6qerv4faJmDpLV6AsoE9GBmyunAU3MHKL1DvkOfZseIXbB0KFtg297FVPcRRN0FrzvytzPgnXaLntAU4cubQoenafB3KDOBzRdLHvlUnkCxqoEBh17NysvICzQfhhmaB96ew3hBiWP0nwl4SCgAxVeFs861Ud4tR53ih-HBxd7R-Xx6dfp3uS41LyhqYSWKiZabAjBRui6ZYRrTY2mjaZKMENFjmoQteYwbinwFoRRLcaGVZoItlO8vdVd9j7KtatREiEIzb2pMzG9JYxXV3IZ7EKFG-mVlX8DPsykCtnCHmTbth3UQjBcqUrXSpGOYWqI5rWg9bjJWp_Xuw3tAowGl4LqN0Q3V5ydy5m_lpXgDRYrgQ9rgeB_DhCTXNiooc8mgx9W5-YNp6LCPKPv_kEfv92amql8Aes6n_fVK1E5qaqKcSYakqnRI1RuBhZW5y_Y2RzfSPi4kZCZBL_STA0xyun59_9nTy832fcP2DmoPs2j74eUP2vcBKtbUAcfY4Du3mSC5aqC7tyQqwqS6wrKaW8ePtB90l3JsD8VKBWg</recordid><startdate>20160226</startdate><enddate>20160226</enddate><creator>Aliuos, Pooyan</creator><creator>Schulze, Jennifer</creator><creator>Schomaker, Markus</creator><creator>Reuter, Günter</creator><creator>Stolle, Stefan R O</creator><creator>Werner, Darja</creator><creator>Ripken, Tammo</creator><creator>Lenarz, Thomas</creator><creator>Warnecke, Athanasia</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160226</creationdate><title>Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear</title><author>Aliuos, Pooyan ; Schulze, Jennifer ; Schomaker, Markus ; Reuter, Günter ; Stolle, Stefan R O ; Werner, Darja ; Ripken, Tammo ; Lenarz, Thomas ; Warnecke, Athanasia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-eb2a37b0d110d7c5b316cc2dc29c2a73d277c5ce75c6e8b2e6be7dab00d34c173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Atomic force microscopy</topic><topic>Beads</topic><topic>Biology and Life Sciences</topic><topic>Biomedical materials</topic><topic>Brain research</topic><topic>Brain-derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - 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However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated.
Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB.
Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release.
Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26918945</pmid><doi>10.1371/journal.pone.0150057</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-02, Vol.11 (2), p.e0150057-e0150057 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1771271295 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adhesion Animals Atomic force microscopy Beads Biology and Life Sciences Biomedical materials Brain research Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - administration & dosage Brain-Derived Neurotrophic Factor - genetics Cell Survival - drug effects Coating effects Cochlea Cochlear Implants Cultivation Cytotoxicity Data processing Drug delivery Drug Delivery Systems Drug Implants Ear, Inner - drug effects Electrodes Electromagnetic fields Emulsion polymerization Fibroblasts Genetic modification Hair Hearing impairment Hearing loss Inner ear Internalization Labeling Labelling Magnetics Medical schools Medicine and Health Sciences Mice Microscopy Neurons Neuroprotection NIH 3T3 Cells Otolaryngology Physical Sciences Physiological aspects Research and Analysis Methods Signal transduction Studies Surgery Surgical implants Transplants & implants |
| title | Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T06%3A37%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Magnetic%20Beads%20Enhance%20Adhesion%20of%20NIH%203T3%20Fibroblasts:%20A%20Proof-of-Principle%20In%20Vitro%20Study%20for%20Implant-Mediated%20Long-Term%20Drug%20Delivery%20to%20the%20Inner%20Ear&rft.jtitle=PloS%20one&rft.au=Aliuos,%20Pooyan&rft.date=2016-02-26&rft.volume=11&rft.issue=2&rft.spage=e0150057&rft.epage=e0150057&rft.pages=e0150057-e0150057&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0150057&rft_dat=%3Cgale_plos_%3EA444363791%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1771271295&rft_id=info:pmid/26918945&rft_galeid=A444363791&rft_doaj_id=oai_doaj_org_article_bbbfe577304a4c5aa1f302d1c6572589&rfr_iscdi=true |