Effects of Methane-Rich Saline on the Capability of One-Time Exhaustive Exercise in Male SD Rats
To explore the effects of methane-rich saline (CH4 saline) on the capability of one-time exhaustive exercise in male SD rats. Thirty rats were equally divided into to three groups at random: control group (C), placebo group (P) and methane saline group (M). Rats in M group underwent intraperitoneal...
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| description | To explore the effects of methane-rich saline (CH4 saline) on the capability of one-time exhaustive exercise in male SD rats.
Thirty rats were equally divided into to three groups at random: control group (C), placebo group (P) and methane saline group (M). Rats in M group underwent intraperitoneal injection of CH4 saline, and the other two groups simultaneously underwent intraperitoneal injection of normal saline. Then, the exercise capability of rats was tested through one-time exhaustive treadmill exercise except C group. Exercise time and body weight were recorded before and after one-time exhaustive exercise. After exhaustive exercise, the blood and gastrocnemius samples were collected from all rats to detect biochemical parameters in different methods.
It was found that the treadmill running time was significantly longer in rats treated with CH4 saline. At the same time, CH4 saline reduced the elevation of LD and UN in blood caused by one-time exhaustive exercise. The low level of blood glucose induced by exhaustive exercise was also normalized by CH4 saline. Also CH4 saline lowered the level of CK in plasma. Furthermore, this research indicated that CH4 saline markedly increased the volume of T-AOC in plasma and alleviated the peak of TNF-α in both plasma and gastrocnemius. From H&E staining, CH4 saline effectively improved exercise-induced structural damage in gastrocnemius.
CH4 saline could enhance exercise capacity in male SD rats through increase of glucose aerobic oxidation, improvement of metabolic clearance and decrease of exhaustive exercise-induced gastrocnemius injury. |
| doi_str_mv | 10.1371/journal.pone.0150925 |
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Thirty rats were equally divided into to three groups at random: control group (C), placebo group (P) and methane saline group (M). Rats in M group underwent intraperitoneal injection of CH4 saline, and the other two groups simultaneously underwent intraperitoneal injection of normal saline. Then, the exercise capability of rats was tested through one-time exhaustive treadmill exercise except C group. Exercise time and body weight were recorded before and after one-time exhaustive exercise. After exhaustive exercise, the blood and gastrocnemius samples were collected from all rats to detect biochemical parameters in different methods.
It was found that the treadmill running time was significantly longer in rats treated with CH4 saline. At the same time, CH4 saline reduced the elevation of LD and UN in blood caused by one-time exhaustive exercise. The low level of blood glucose induced by exhaustive exercise was also normalized by CH4 saline. Also CH4 saline lowered the level of CK in plasma. Furthermore, this research indicated that CH4 saline markedly increased the volume of T-AOC in plasma and alleviated the peak of TNF-α in both plasma and gastrocnemius. From H&E staining, CH4 saline effectively improved exercise-induced structural damage in gastrocnemius.
CH4 saline could enhance exercise capacity in male SD rats through increase of glucose aerobic oxidation, improvement of metabolic clearance and decrease of exhaustive exercise-induced gastrocnemius injury.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0150925</identifier><identifier>PMID: 26942576</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aerobic capacity ; Animals ; Antioxidants - metabolism ; Biology ; Biology and Life Sciences ; Blood ; Blood Glucose - metabolism ; Body weight ; Body Weight - drug effects ; Creatine Kinase - blood ; Cytokines - blood ; Diabetes ; Diabetic retinopathy ; Exercise ; Fitness equipment ; Free radicals ; Glucose ; Inflammation ; Injection ; Ischemia ; Kinesiology ; Laboratory animals ; Lactic Acid - blood ; Low level ; Male ; Malondialdehyde - blood ; Medicine ; Medicine and Health Sciences ; Methane ; Methane - pharmacology ; Muscle, Skeletal - drug effects ; Musculoskeletal system ; Natural gas utilities ; Nitrogen - blood ; Nutrition research ; Oxidation ; Oxidative stress ; Peroxidase - blood ; Physical Conditioning, Animal ; Physical Sciences ; Physical training ; Physiological aspects ; Physiology ; Rats ; Rats, Sprague-Dawley ; Rodents ; Running ; Saline solution ; Saline solutions ; Sodium Chloride - pharmacology ; Structural damage ; Superoxide Dismutase - blood ; Time Factors ; Tumor necrosis factor-α ; Urea - blood</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0150925-e0150925</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Xin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Xin et al 2016 Xin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-483d2b9b17fb7c63e292a9fa4c1b643fcc7f355797215a87d45d3c2a6d4edd543</citedby><cites>FETCH-LOGICAL-c692t-483d2b9b17fb7c63e292a9fa4c1b643fcc7f355797215a87d45d3c2a6d4edd543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778848/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778848/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26942576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>López Lluch, Guillermo</contributor><creatorcontrib>Xin, Lei</creatorcontrib><creatorcontrib>Sun, Xuejun</creatorcontrib><creatorcontrib>Lou, Shujie</creatorcontrib><title>Effects of Methane-Rich Saline on the Capability of One-Time Exhaustive Exercise in Male SD Rats</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>To explore the effects of methane-rich saline (CH4 saline) on the capability of one-time exhaustive exercise in male SD rats.
Thirty rats were equally divided into to three groups at random: control group (C), placebo group (P) and methane saline group (M). Rats in M group underwent intraperitoneal injection of CH4 saline, and the other two groups simultaneously underwent intraperitoneal injection of normal saline. Then, the exercise capability of rats was tested through one-time exhaustive treadmill exercise except C group. Exercise time and body weight were recorded before and after one-time exhaustive exercise. After exhaustive exercise, the blood and gastrocnemius samples were collected from all rats to detect biochemical parameters in different methods.
It was found that the treadmill running time was significantly longer in rats treated with CH4 saline. At the same time, CH4 saline reduced the elevation of LD and UN in blood caused by one-time exhaustive exercise. The low level of blood glucose induced by exhaustive exercise was also normalized by CH4 saline. Also CH4 saline lowered the level of CK in plasma. Furthermore, this research indicated that CH4 saline markedly increased the volume of T-AOC in plasma and alleviated the peak of TNF-α in both plasma and gastrocnemius. From H&E staining, CH4 saline effectively improved exercise-induced structural damage in gastrocnemius.
CH4 saline could enhance exercise capacity in male SD rats through increase of glucose aerobic oxidation, improvement of metabolic clearance and decrease of exhaustive exercise-induced gastrocnemius injury.</description><subject>Aerobic capacity</subject><subject>Animals</subject><subject>Antioxidants - metabolism</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood Glucose - metabolism</subject><subject>Body weight</subject><subject>Body Weight - drug effects</subject><subject>Creatine Kinase - blood</subject><subject>Cytokines - blood</subject><subject>Diabetes</subject><subject>Diabetic retinopathy</subject><subject>Exercise</subject><subject>Fitness equipment</subject><subject>Free radicals</subject><subject>Glucose</subject><subject>Inflammation</subject><subject>Injection</subject><subject>Ischemia</subject><subject>Kinesiology</subject><subject>Laboratory animals</subject><subject>Lactic Acid - blood</subject><subject>Low level</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methane</subject><subject>Methane - pharmacology</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Musculoskeletal system</subject><subject>Natural gas utilities</subject><subject>Nitrogen - blood</subject><subject>Nutrition research</subject><subject>Oxidation</subject><subject>Oxidative stress</subject><subject>Peroxidase - blood</subject><subject>Physical Conditioning, Animal</subject><subject>Physical Sciences</subject><subject>Physical training</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Running</subject><subject>Saline solution</subject><subject>Saline solutions</subject><subject>Sodium Chloride - pharmacology</subject><subject>Structural damage</subject><subject>Superoxide Dismutase - blood</subject><subject>Time Factors</subject><subject>Tumor necrosis factor-α</subject><subject>Urea - 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metabolism</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood Glucose - metabolism</topic><topic>Body weight</topic><topic>Body Weight - drug effects</topic><topic>Creatine Kinase - blood</topic><topic>Cytokines - blood</topic><topic>Diabetes</topic><topic>Diabetic retinopathy</topic><topic>Exercise</topic><topic>Fitness equipment</topic><topic>Free radicals</topic><topic>Glucose</topic><topic>Inflammation</topic><topic>Injection</topic><topic>Ischemia</topic><topic>Kinesiology</topic><topic>Laboratory animals</topic><topic>Lactic Acid - blood</topic><topic>Low level</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Methane</topic><topic>Methane - pharmacology</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Musculoskeletal system</topic><topic>Natural gas utilities</topic><topic>Nitrogen - blood</topic><topic>Nutrition research</topic><topic>Oxidation</topic><topic>Oxidative stress</topic><topic>Peroxidase - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xin, Lei</au><au>Sun, Xuejun</au><au>Lou, Shujie</au><au>López Lluch, Guillermo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Methane-Rich Saline on the Capability of One-Time Exhaustive Exercise in Male SD Rats</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-04</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0150925</spage><epage>e0150925</epage><pages>e0150925-e0150925</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To explore the effects of methane-rich saline (CH4 saline) on the capability of one-time exhaustive exercise in male SD rats.
Thirty rats were equally divided into to three groups at random: control group (C), placebo group (P) and methane saline group (M). Rats in M group underwent intraperitoneal injection of CH4 saline, and the other two groups simultaneously underwent intraperitoneal injection of normal saline. Then, the exercise capability of rats was tested through one-time exhaustive treadmill exercise except C group. Exercise time and body weight were recorded before and after one-time exhaustive exercise. After exhaustive exercise, the blood and gastrocnemius samples were collected from all rats to detect biochemical parameters in different methods.
It was found that the treadmill running time was significantly longer in rats treated with CH4 saline. At the same time, CH4 saline reduced the elevation of LD and UN in blood caused by one-time exhaustive exercise. The low level of blood glucose induced by exhaustive exercise was also normalized by CH4 saline. Also CH4 saline lowered the level of CK in plasma. Furthermore, this research indicated that CH4 saline markedly increased the volume of T-AOC in plasma and alleviated the peak of TNF-α in both plasma and gastrocnemius. From H&E staining, CH4 saline effectively improved exercise-induced structural damage in gastrocnemius.
CH4 saline could enhance exercise capacity in male SD rats through increase of glucose aerobic oxidation, improvement of metabolic clearance and decrease of exhaustive exercise-induced gastrocnemius injury.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26942576</pmid><doi>10.1371/journal.pone.0150925</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0150925-e0150925 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aerobic capacity Animals Antioxidants - metabolism Biology Biology and Life Sciences Blood Blood Glucose - metabolism Body weight Body Weight - drug effects Creatine Kinase - blood Cytokines - blood Diabetes Diabetic retinopathy Exercise Fitness equipment Free radicals Glucose Inflammation Injection Ischemia Kinesiology Laboratory animals Lactic Acid - blood Low level Male Malondialdehyde - blood Medicine Medicine and Health Sciences Methane Methane - pharmacology Muscle, Skeletal - drug effects Musculoskeletal system Natural gas utilities Nitrogen - blood Nutrition research Oxidation Oxidative stress Peroxidase - blood Physical Conditioning, Animal Physical Sciences Physical training Physiological aspects Physiology Rats Rats, Sprague-Dawley Rodents Running Saline solution Saline solutions Sodium Chloride - pharmacology Structural damage Superoxide Dismutase - blood Time Factors Tumor necrosis factor-α Urea - blood |
| title | Effects of Methane-Rich Saline on the Capability of One-Time Exhaustive Exercise in Male SD Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T01%3A14%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20Methane-Rich%20Saline%20on%20the%20Capability%20of%20One-Time%20Exhaustive%20Exercise%20in%20Male%20SD%20Rats&rft.jtitle=PloS%20one&rft.au=Xin,%20Lei&rft.date=2016-03-04&rft.volume=11&rft.issue=3&rft.spage=e0150925&rft.epage=e0150925&rft.pages=e0150925-e0150925&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0150925&rft_dat=%3Cgale_plos_%3EA445095597%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770505068&rft_id=info:pmid/26942576&rft_galeid=A445095597&rft_doaj_id=oai_doaj_org_article_e4d6865931b14a78a2c8325478eacfce&rfr_iscdi=true |