Effect of Melatonin in Epithelial Mesenchymal Transition Markers and Invasive Properties of Breast Cancer Stem Cells of Canine and Human Cell Lines
Cancer stem cells (CSCs) have been associated with metastasis and therapeutic resistance and can be generated via epithelial mesenchymal transition (EMT). Some studies suggest that the hormone melatonin acts in CSCs and may participate in the inhibition of the EMT. The objectives of this study were...
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| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0150407-e0150407 |
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| creator | Gonçalves, Naiane do Nascimento Colombo, Jucimara Lopes, Juliana Ramos Gelaleti, Gabriela Bottaro Moschetta, Marina Gobbe Sonehara, Nathália Martins Hellmén, Eva Zanon, Caroline de Freitas Oliani, Sônia Maria Zuccari, Debora Aparecida Pires de Campos |
| description | Cancer stem cells (CSCs) have been associated with metastasis and therapeutic resistance and can be generated via epithelial mesenchymal transition (EMT). Some studies suggest that the hormone melatonin acts in CSCs and may participate in the inhibition of the EMT. The objectives of this study were to evaluate the formation of mammospheres from the canine and human breast cancer cell lines, CMT-U229 and MCF-7, and the effects of melatonin treatment on the modulation of stem cell and EMT molecular markers: OCT4, E-cadherin, N-cadherin and vimentin, as well as on cell viability and invasiveness of the cells from mammospheres. The CMT-U229 and MCF-7 cell lines were subjected to three-dimensional culture in special medium for stem cells. The phenotype of mammospheres was first evaluated by flow cytometry (CD44(+)/CD24(low/-) marking). Cell viability was measured by MTT colorimetric assay and the expression of the proteins OCT4, E-cadherin, N-cadherin and vimentin was evaluated by immunofluorescence and quantified by optical densitometry. The analysis of cell migration and invasion was performed in Boyden Chamber. Flow cytometry proved the stem cell phenotype with CD44(+)/CD24(low/-) positive marking for both cell lines. Cell viability of CMT-U229 and MCF-7 cells was reduced after treatment with 1mM melatonin for 24 h (P |
| doi_str_mv | 10.1371/journal.pone.0150407 |
| format | Article |
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Some studies suggest that the hormone melatonin acts in CSCs and may participate in the inhibition of the EMT. The objectives of this study were to evaluate the formation of mammospheres from the canine and human breast cancer cell lines, CMT-U229 and MCF-7, and the effects of melatonin treatment on the modulation of stem cell and EMT molecular markers: OCT4, E-cadherin, N-cadherin and vimentin, as well as on cell viability and invasiveness of the cells from mammospheres. The CMT-U229 and MCF-7 cell lines were subjected to three-dimensional culture in special medium for stem cells. The phenotype of mammospheres was first evaluated by flow cytometry (CD44(+)/CD24(low/-) marking). Cell viability was measured by MTT colorimetric assay and the expression of the proteins OCT4, E-cadherin, N-cadherin and vimentin was evaluated by immunofluorescence and quantified by optical densitometry. The analysis of cell migration and invasion was performed in Boyden Chamber. Flow cytometry proved the stem cell phenotype with CD44(+)/CD24(low/-) positive marking for both cell lines. Cell viability of CMT-U229 and MCF-7 cells was reduced after treatment with 1mM melatonin for 24 h (P<0.05). Immunofluorescence staining showed increased E-cadherin expression (P<0.05) and decreased expression of OCT4, N-cadherin and vimentin (P<0.05) in both cell lines after treatment with 1 mM melatonin for 24 hours. Moreover, treatment with melatonin was able to reduce cell migration and invasion in both cell lines when compared to control group (P<0.05). Our results demonstrate that melatonin shows an inhibitory role in the viability and invasiveness of breast cancer mammospheres as well as in modulating the expression of proteins related to EMT in breast CSCs, suggesting its potential anti-metastatic role in canine and human breast cancer cell lines.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0150407</identifier><identifier>PMID: 26934679</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Apoptosis ; Biology and Life Sciences ; Biomarkers ; Biotechnology ; Boyden chamber ; Breast - drug effects ; Breast - pathology ; Breast cancer ; Breast Neoplasms - drug therapy ; Breast Neoplasms - pathology ; Cancer ; CD44 antigen ; Cell adhesion & migration ; Cell and Molecular Biology ; Cell culture ; Cell Line, Tumor ; Cell migration ; Cell Movement - drug effects ; Cell Survival - drug effects ; Cell- och molekylärbiologi ; Colorimetry ; Complications and side effects ; Cytometry ; Densitometers ; Densitometry ; Development and progression ; Dogs ; Dosage and administration ; Drug therapy ; E-cadherin ; Epithelial-Mesenchymal Transition - drug effects ; Female ; Flow cytometry ; Genetic aspects ; Humans ; Immunofluorescence ; Invasiveness ; Kinases ; Marking ; MCF-7 Cells ; Medicine and Health Sciences ; Melatonin ; Melatonin - pharmacology ; Mesenchyme ; Metastases ; N-Cadherin ; Neoplasm Invasiveness - pathology ; Neoplasm Invasiveness - prevention & control ; Neoplastic Stem Cells - drug effects ; Neoplastic Stem Cells - pathology ; Oct-4 protein ; Physiological aspects ; Proteins ; Research and Analysis Methods ; Stem cells ; Tumor cell lines ; Vimentin</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0150407-e0150407</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Gonçalves et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Gonçalves et al 2016 Gonçalves et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c796t-c77ce143430a0f18491a9bb2da9319d82f59fbae0716ccbd9f374adeed8eb8ce3</citedby><cites>FETCH-LOGICAL-c796t-c77ce143430a0f18491a9bb2da9319d82f59fbae0716ccbd9f374adeed8eb8ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774906/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774906/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26934679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/83271$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Schmitt, Fernando</contributor><creatorcontrib>Gonçalves, Naiane do Nascimento</creatorcontrib><creatorcontrib>Colombo, Jucimara</creatorcontrib><creatorcontrib>Lopes, Juliana Ramos</creatorcontrib><creatorcontrib>Gelaleti, Gabriela Bottaro</creatorcontrib><creatorcontrib>Moschetta, Marina Gobbe</creatorcontrib><creatorcontrib>Sonehara, Nathália Martins</creatorcontrib><creatorcontrib>Hellmén, Eva</creatorcontrib><creatorcontrib>Zanon, Caroline de Freitas</creatorcontrib><creatorcontrib>Oliani, Sônia Maria</creatorcontrib><creatorcontrib>Zuccari, Debora Aparecida Pires de Campos</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Effect of Melatonin in Epithelial Mesenchymal Transition Markers and Invasive Properties of Breast Cancer Stem Cells of Canine and Human Cell Lines</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cancer stem cells (CSCs) have been associated with metastasis and therapeutic resistance and can be generated via epithelial mesenchymal transition (EMT). Some studies suggest that the hormone melatonin acts in CSCs and may participate in the inhibition of the EMT. The objectives of this study were to evaluate the formation of mammospheres from the canine and human breast cancer cell lines, CMT-U229 and MCF-7, and the effects of melatonin treatment on the modulation of stem cell and EMT molecular markers: OCT4, E-cadherin, N-cadherin and vimentin, as well as on cell viability and invasiveness of the cells from mammospheres. The CMT-U229 and MCF-7 cell lines were subjected to three-dimensional culture in special medium for stem cells. The phenotype of mammospheres was first evaluated by flow cytometry (CD44(+)/CD24(low/-) marking). Cell viability was measured by MTT colorimetric assay and the expression of the proteins OCT4, E-cadherin, N-cadherin and vimentin was evaluated by immunofluorescence and quantified by optical densitometry. The analysis of cell migration and invasion was performed in Boyden Chamber. Flow cytometry proved the stem cell phenotype with CD44(+)/CD24(low/-) positive marking for both cell lines. Cell viability of CMT-U229 and MCF-7 cells was reduced after treatment with 1mM melatonin for 24 h (P<0.05). Immunofluorescence staining showed increased E-cadherin expression (P<0.05) and decreased expression of OCT4, N-cadherin and vimentin (P<0.05) in both cell lines after treatment with 1 mM melatonin for 24 hours. Moreover, treatment with melatonin was able to reduce cell migration and invasion in both cell lines when compared to control group (P<0.05). Our results demonstrate that melatonin shows an inhibitory role in the viability and invasiveness of breast cancer mammospheres as well as in modulating the expression of proteins related to EMT in breast CSCs, suggesting its potential anti-metastatic role in canine and human breast cancer cell lines.</description><subject>Analysis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Boyden chamber</subject><subject>Breast - drug effects</subject><subject>Breast - pathology</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>CD44 antigen</subject><subject>Cell adhesion & migration</subject><subject>Cell and Molecular Biology</subject><subject>Cell culture</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - 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pathology</subject><subject>Neoplasm Invasiveness - prevention & control</subject><subject>Neoplastic Stem Cells - drug effects</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Oct-4 protein</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Stem cells</subject><subject>Tumor cell lines</subject><subject>Vimentin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9Fu0zAUhiMEYmPwBggiISG4aLFjN45vJo1qsEqbhtjg1jpxTlqPxC52Mthz8MI4bTctaBcoUWwdf_9vnxOfJHlJyZQyQT9cud5baKZrZ3FK6IxwIh4l-1SybJJnhD2-N99LnoVwRciMFXn-NNnLcsl4LuR-8ue4rlF3qavTM2ygc9bYNL7Ha9OtsDHQxHhAq1c3bZxferDBdMbZ9Az8D_QhBVulC3sNwVxj-sW7NfrOYBgcP3qE0KVzsBp9etFhm86xaTZrMWgsbtQnfQt2s5Kexlh4njypoQn4YjceJN8-HV_OTyan558X86PTiRYy7-JXaKSccUaA1LTgkoIsy6wCyaisiqyeyboEJILmWpeVrJngUCFWBZaFRnaQvN76rhsX1K6eQVGRSylzkRWRWGyJysGVWnvTgr9RDozaBJxfKojZ6gaVqLN4miwXpRa8xLKMJsgLVrFsNgMy7DbZeoVfuO7LkVto-hL8MKiAqmCZoJE_3J2uL1usNNrOQzOSjVesWamlu1ZcCC5JHg3e7Qy8-9lj6FRrgo5VBouuH_IUpMhnhRARffMP-nA1dtQSYsLG1i7uqwdTdcQ5LwSnJIvU9AEqPhW2RsfLWpsYHwnejwSR6fB3t4Q-BLW4-Pr_7Pn3Mfv2HrtCaLpVcE0_3N4wBvkW1N6F4LG-KzIlaui122qoodfUrtei7NX9H3Qnum0u9hcn_CbO</recordid><startdate>20160302</startdate><enddate>20160302</enddate><creator>Gonçalves, Naiane do Nascimento</creator><creator>Colombo, Jucimara</creator><creator>Lopes, Juliana Ramos</creator><creator>Gelaleti, Gabriela Bottaro</creator><creator>Moschetta, Marina Gobbe</creator><creator>Sonehara, Nathália Martins</creator><creator>Hellmén, Eva</creator><creator>Zanon, Caroline de Freitas</creator><creator>Oliani, Sônia Maria</creator><creator>Zuccari, Debora Aparecida Pires de Campos</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DOA</scope></search><sort><creationdate>20160302</creationdate><title>Effect of Melatonin in Epithelial Mesenchymal Transition Markers and Invasive Properties of Breast Cancer Stem Cells of Canine and Human Cell Lines</title><author>Gonçalves, Naiane do Nascimento ; Colombo, Jucimara ; Lopes, Juliana Ramos ; Gelaleti, Gabriela Bottaro ; Moschetta, Marina Gobbe ; Sonehara, Nathália Martins ; Hellmén, Eva ; Zanon, Caroline de Freitas ; Oliani, Sônia Maria ; Zuccari, Debora Aparecida Pires de Campos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c796t-c77ce143430a0f18491a9bb2da9319d82f59fbae0716ccbd9f374adeed8eb8ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Boyden chamber</topic><topic>Breast - 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pharmacology</topic><topic>Mesenchyme</topic><topic>Metastases</topic><topic>N-Cadherin</topic><topic>Neoplasm Invasiveness - pathology</topic><topic>Neoplasm Invasiveness - prevention & control</topic><topic>Neoplastic Stem Cells - drug effects</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Oct-4 protein</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Stem cells</topic><topic>Tumor cell lines</topic><topic>Vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonçalves, Naiane do Nascimento</creatorcontrib><creatorcontrib>Colombo, Jucimara</creatorcontrib><creatorcontrib>Lopes, Juliana Ramos</creatorcontrib><creatorcontrib>Gelaleti, Gabriela Bottaro</creatorcontrib><creatorcontrib>Moschetta, Marina Gobbe</creatorcontrib><creatorcontrib>Sonehara, Nathália Martins</creatorcontrib><creatorcontrib>Hellmén, Eva</creatorcontrib><creatorcontrib>Zanon, Caroline de Freitas</creatorcontrib><creatorcontrib>Oliani, Sônia Maria</creatorcontrib><creatorcontrib>Zuccari, Debora Aparecida Pires de Campos</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonçalves, Naiane do Nascimento</au><au>Colombo, Jucimara</au><au>Lopes, Juliana Ramos</au><au>Gelaleti, Gabriela Bottaro</au><au>Moschetta, Marina Gobbe</au><au>Sonehara, Nathália Martins</au><au>Hellmén, Eva</au><au>Zanon, Caroline de Freitas</au><au>Oliani, Sônia Maria</au><au>Zuccari, Debora Aparecida Pires de Campos</au><au>Schmitt, Fernando</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Melatonin in Epithelial Mesenchymal Transition Markers and Invasive Properties of Breast Cancer Stem Cells of Canine and Human Cell Lines</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-02</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0150407</spage><epage>e0150407</epage><pages>e0150407-e0150407</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cancer stem cells (CSCs) have been associated with metastasis and therapeutic resistance and can be generated via epithelial mesenchymal transition (EMT). Some studies suggest that the hormone melatonin acts in CSCs and may participate in the inhibition of the EMT. The objectives of this study were to evaluate the formation of mammospheres from the canine and human breast cancer cell lines, CMT-U229 and MCF-7, and the effects of melatonin treatment on the modulation of stem cell and EMT molecular markers: OCT4, E-cadherin, N-cadherin and vimentin, as well as on cell viability and invasiveness of the cells from mammospheres. The CMT-U229 and MCF-7 cell lines were subjected to three-dimensional culture in special medium for stem cells. The phenotype of mammospheres was first evaluated by flow cytometry (CD44(+)/CD24(low/-) marking). Cell viability was measured by MTT colorimetric assay and the expression of the proteins OCT4, E-cadherin, N-cadherin and vimentin was evaluated by immunofluorescence and quantified by optical densitometry. The analysis of cell migration and invasion was performed in Boyden Chamber. Flow cytometry proved the stem cell phenotype with CD44(+)/CD24(low/-) positive marking for both cell lines. Cell viability of CMT-U229 and MCF-7 cells was reduced after treatment with 1mM melatonin for 24 h (P<0.05). Immunofluorescence staining showed increased E-cadherin expression (P<0.05) and decreased expression of OCT4, N-cadherin and vimentin (P<0.05) in both cell lines after treatment with 1 mM melatonin for 24 hours. Moreover, treatment with melatonin was able to reduce cell migration and invasion in both cell lines when compared to control group (P<0.05). Our results demonstrate that melatonin shows an inhibitory role in the viability and invasiveness of breast cancer mammospheres as well as in modulating the expression of proteins related to EMT in breast CSCs, suggesting its potential anti-metastatic role in canine and human breast cancer cell lines.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26934679</pmid><doi>10.1371/journal.pone.0150407</doi><tpages>e0150407</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0150407-e0150407 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1769996728 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Apoptosis Biology and Life Sciences Biomarkers Biotechnology Boyden chamber Breast - drug effects Breast - pathology Breast cancer Breast Neoplasms - drug therapy Breast Neoplasms - pathology Cancer CD44 antigen Cell adhesion & migration Cell and Molecular Biology Cell culture Cell Line, Tumor Cell migration Cell Movement - drug effects Cell Survival - drug effects Cell- och molekylärbiologi Colorimetry Complications and side effects Cytometry Densitometers Densitometry Development and progression Dogs Dosage and administration Drug therapy E-cadherin Epithelial-Mesenchymal Transition - drug effects Female Flow cytometry Genetic aspects Humans Immunofluorescence Invasiveness Kinases Marking MCF-7 Cells Medicine and Health Sciences Melatonin Melatonin - pharmacology Mesenchyme Metastases N-Cadherin Neoplasm Invasiveness - pathology Neoplasm Invasiveness - prevention & control Neoplastic Stem Cells - drug effects Neoplastic Stem Cells - pathology Oct-4 protein Physiological aspects Proteins Research and Analysis Methods Stem cells Tumor cell lines Vimentin |
| title | Effect of Melatonin in Epithelial Mesenchymal Transition Markers and Invasive Properties of Breast Cancer Stem Cells of Canine and Human Cell Lines |
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