Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported th...
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| creator | Takemoto, Noriaki Yoshimura, Takuji Miyazaki, Satsuki Tashiro, Fumi Miyazaki, Jun-ichi |
| description | The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice. |
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These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0150390</identifier><identifier>PMID: 26930067</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Amino Acid Sequence ; Animals ; Argonaute Proteins - genetics ; Argonaute Proteins - metabolism ; Biology and Life Sciences ; Biosynthesis ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell Line ; Cells, Cultured ; Drosophila ; Female ; Fluorescent Antibody Technique ; Gene expression ; Gene Expression Regulation, Developmental ; Genetic aspects ; Germ Cells - growth & development ; Germ Cells - metabolism ; Immunoblotting ; Immunoglobulins ; Insects ; Male ; Mammals ; Medicine ; Medicine and Health Sciences ; Mice ; Mice, 129 Strain ; Mice, Inbred C57BL ; Mice, Knockout ; Molecular Sequence Data ; Physiological aspects ; Protein Binding ; Proteins ; Proteins - genetics ; Proteins - metabolism ; Research and Analysis Methods ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; Rodents ; Sequence Homology, Amino Acid ; Sperm ; Spermatids ; Spermatids - growth & development ; Spermatids - metabolism ; Spermatogenesis ; Spermatogenesis - genetics ; Stem cells ; Testis - cytology ; Testis - metabolism ; University graduates ; Zinc</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0150390-e0150390</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Takemoto et al. 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These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice.</description><subject>Acids</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Argonaute Proteins - genetics</subject><subject>Argonaute Proteins - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Drosophila</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genetic aspects</subject><subject>Germ Cells - growth & development</subject><subject>Germ Cells - metabolism</subject><subject>Immunoblotting</subject><subject>Immunoglobulins</subject><subject>Insects</subject><subject>Male</subject><subject>Mammals</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, 129 Strain</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Molecular Sequence Data</subject><subject>Physiological aspects</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takemoto, Noriaki</au><au>Yoshimura, Takuji</au><au>Miyazaki, Satsuki</au><au>Tashiro, Fumi</au><au>Miyazaki, Jun-ichi</au><au>Yang, Wan-Xi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0150390</spage><epage>e0150390</epage><pages>e0150390-e0150390</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26930067</pmid><doi>10.1371/journal.pone.0150390</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Amino Acid Sequence Animals Argonaute Proteins - genetics Argonaute Proteins - metabolism Biology and Life Sciences Biosynthesis Carrier Proteins - genetics Carrier Proteins - metabolism Cell Line Cells, Cultured Drosophila Female Fluorescent Antibody Technique Gene expression Gene Expression Regulation, Developmental Genetic aspects Germ Cells - growth & development Germ Cells - metabolism Immunoblotting Immunoglobulins Insects Male Mammals Medicine Medicine and Health Sciences Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Knockout Molecular Sequence Data Physiological aspects Protein Binding Proteins Proteins - genetics Proteins - metabolism Research and Analysis Methods Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA Rodents Sequence Homology, Amino Acid Sperm Spermatids Spermatids - growth & development Spermatids - metabolism Spermatogenesis Spermatogenesis - genetics Stem cells Testis - cytology Testis - metabolism University graduates Zinc |
| title | Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis |
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