Identification and Comparative Study of Chemosensory Genes Related to Host Selection by Legs Transcriptome Analysis in the Tea Geometrid Ectropis obliqua
Host selection by female moths is fundamental to the survival of their larvae. Detecting and perceiving the non-volatile chemicals of the plant surface involved in gustatory detection determine the host preference. In many lepidopteran species, tarsal chemosensilla are sensitive to non-volatile chem...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-03, Vol.11 (3), p.e0149591-e0149591 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0149591 |
|---|---|
| container_issue | 3 |
| container_start_page | e0149591 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Ma, Long Li, Zhao-Qun Bian, Lei Cai, Xiao-Ming Luo, Zong-Xiu Zhang, Yong-Jun Chen, Zong-Mao |
| description | Host selection by female moths is fundamental to the survival of their larvae. Detecting and perceiving the non-volatile chemicals of the plant surface involved in gustatory detection determine the host preference. In many lepidopteran species, tarsal chemosensilla are sensitive to non-volatile chemicals and responsible for taste detection. The tea geometrid Ectropis obliqua is one devastating chewing pest selectively feeding on limited plants, requiring the specialized sensors to forage certain host for oviposition. In present study, we revealed the distribution of chemosensilla in the ventral side of female fifth tarsomere in E. obliqua. To investigate its molecular mechanism of gustatory perception, we performed HiSeq 2500 sequencing of the male- and female- legs transcriptome and identified 24 candidate odorant binding proteins (OBPs), 21 chemosensory proteins (CSPs), 2 sensory neuron membrane proteins (SNMPs), 3 gustatory receptors (GRs) and 4 odorant receptors (ORs). Several leg-specific or enriched chemosensory genes were screened by tissue expression analysis, and clustered with functionally validated genes from other moths, suggesting the potential involvement in taste sensation or other physiological processes. The RPKM value analysis revealed that 9 EoblOBPs showed sex discrepancy in the leg expression, 8 being up-regulated in female and only 1 being over expressed in male. These female-biased EoblOBPs indicated an ecological adaption related with host-seeking and oviposition behaviors. Our work will provide basic knowledge for further studies on the molecular mechanism of gustatory perception, and enlighten a host-selection-based control strategy of insect pests. |
| doi_str_mv | 10.1371/journal.pone.0149591 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1769627927</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A444874220</galeid><doaj_id>oai_doaj_org_article_37d31d28a20e496caebe22505f71a529</doaj_id><sourcerecordid>A444874220</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-dda996075ef95cb4d46161ec1ddc64fa3253e93f7c09ad4957e07480c2195b13</originalsourceid><addsrcrecordid>eNqNk9Fq2zAUhs3YWLtsbzA2wWBsF8kk2Zaim0IIXRsIFJqwW6FIx4mKbaWSXJZH2dtOadISj14Mg23pfOeX9OucLPtI8IjknPy4c51vVT3auhZGmBSiFORVdk5EToeM4vz1yf9Z9i6EO4zLfMzY2-yMMpGnETvP_swMtNFWVqtoXYtUa9DUNVvl0_gB0CJ2ZodchaYbaFyANji_Q1fQQkC3UKsIBkWHrl2IaAE16EeV1Q7NYR3Q0qs2aG-30TWAJmm7u2ADsi2KG0BLUEkpRaK3Bl3q6N02Rd2qtvedep-9qVQd4MPxO8iWPy-X0-vh_OZqNp3Mh5oJGofGKCEY5iVUotSrwhSMMAKaGKNZUamcljmIvOIaC2WSSxwwL8ZYUyLKFckH2eeD7LZ2QR5NDZJwJhjlgvJEzA6EcepObr1tlN9Jp6x8nHB-LZWPVtcgc25yYuhYUQyFYFrBCigtcVlxokoqktbFcbVu1YDRyXuv6p5oP9LajVy7B1lwnm6MJYFvRwHv7jsIUTY2aKhr1YLr9vvmmKYXoQn98g_68umO1FqlA9i2cmldvReVk6IoxrygqYAG2egFKj0GGqtTBVY2zfcSvvcSEhPhd1yrLgQ5W9z-P3vzq89-PWE3oOq4Ca7u9mUX-mBxALV3IXionk0mWO4b6MkNuW8geWyglPbp9IKek546Jv8LXrsXcg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1769627927</pqid></control><display><type>article</type><title>Identification and Comparative Study of Chemosensory Genes Related to Host Selection by Legs Transcriptome Analysis in the Tea Geometrid Ectropis obliqua</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Ma, Long ; Li, Zhao-Qun ; Bian, Lei ; Cai, Xiao-Ming ; Luo, Zong-Xiu ; Zhang, Yong-Jun ; Chen, Zong-Mao</creator><contributor>Hull, J Joe</contributor><creatorcontrib>Ma, Long ; Li, Zhao-Qun ; Bian, Lei ; Cai, Xiao-Ming ; Luo, Zong-Xiu ; Zhang, Yong-Jun ; Chen, Zong-Mao ; Hull, J Joe</creatorcontrib><description>Host selection by female moths is fundamental to the survival of their larvae. Detecting and perceiving the non-volatile chemicals of the plant surface involved in gustatory detection determine the host preference. In many lepidopteran species, tarsal chemosensilla are sensitive to non-volatile chemicals and responsible for taste detection. The tea geometrid Ectropis obliqua is one devastating chewing pest selectively feeding on limited plants, requiring the specialized sensors to forage certain host for oviposition. In present study, we revealed the distribution of chemosensilla in the ventral side of female fifth tarsomere in E. obliqua. To investigate its molecular mechanism of gustatory perception, we performed HiSeq 2500 sequencing of the male- and female- legs transcriptome and identified 24 candidate odorant binding proteins (OBPs), 21 chemosensory proteins (CSPs), 2 sensory neuron membrane proteins (SNMPs), 3 gustatory receptors (GRs) and 4 odorant receptors (ORs). Several leg-specific or enriched chemosensory genes were screened by tissue expression analysis, and clustered with functionally validated genes from other moths, suggesting the potential involvement in taste sensation or other physiological processes. The RPKM value analysis revealed that 9 EoblOBPs showed sex discrepancy in the leg expression, 8 being up-regulated in female and only 1 being over expressed in male. These female-biased EoblOBPs indicated an ecological adaption related with host-seeking and oviposition behaviors. Our work will provide basic knowledge for further studies on the molecular mechanism of gustatory perception, and enlighten a host-selection-based control strategy of insect pests.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0149591</identifier><identifier>PMID: 26930056</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Agrotis ipsilon ; Amino Acid Sequence ; Amino acids ; Animals ; Biology ; Biology and Life Sciences ; Butterflies & moths ; Chemicals ; Chemoreception ; Chewing ; Comparative studies ; Drosophila ; Drosophila melanogaster ; Ectropis obliqua ; Female ; Gene expression ; Gene Expression Profiling ; Gene Ontology ; Genes ; Genetic aspects ; Helicoverpa ; Helicoverpa armigera ; Host plants ; Host preferences ; Host selection ; Insect pests ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insects ; Laboratories ; Larvae ; Leg ; Legs ; Lepidoptera ; Ligands ; Male ; Mastication ; Medicine and Health Sciences ; Membrane proteins ; Molecular Sequence Data ; Moths - anatomy & histology ; Moths - chemistry ; Moths - genetics ; Moths - physiology ; Odorant receptors ; Oviposition ; Perception ; Pests ; Pheromones ; Physiological aspects ; Phytochemicals ; Phytophagous insects ; Plants - parasitology ; Proteins ; Receptors ; Receptors, Odorant - chemistry ; Receptors, Odorant - genetics ; Sequence Alignment ; Social Sciences ; Taste ; Taste perception ; Taste receptors ; Tea ; Tissue analysis ; Transcriptome ; Value analysis</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0149591-e0149591</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Ma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Ma et al 2016 Ma et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-dda996075ef95cb4d46161ec1ddc64fa3253e93f7c09ad4957e07480c2195b13</citedby><cites>FETCH-LOGICAL-c692t-dda996075ef95cb4d46161ec1ddc64fa3253e93f7c09ad4957e07480c2195b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773006/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773006/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26930056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hull, J Joe</contributor><creatorcontrib>Ma, Long</creatorcontrib><creatorcontrib>Li, Zhao-Qun</creatorcontrib><creatorcontrib>Bian, Lei</creatorcontrib><creatorcontrib>Cai, Xiao-Ming</creatorcontrib><creatorcontrib>Luo, Zong-Xiu</creatorcontrib><creatorcontrib>Zhang, Yong-Jun</creatorcontrib><creatorcontrib>Chen, Zong-Mao</creatorcontrib><title>Identification and Comparative Study of Chemosensory Genes Related to Host Selection by Legs Transcriptome Analysis in the Tea Geometrid Ectropis obliqua</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Host selection by female moths is fundamental to the survival of their larvae. Detecting and perceiving the non-volatile chemicals of the plant surface involved in gustatory detection determine the host preference. In many lepidopteran species, tarsal chemosensilla are sensitive to non-volatile chemicals and responsible for taste detection. The tea geometrid Ectropis obliqua is one devastating chewing pest selectively feeding on limited plants, requiring the specialized sensors to forage certain host for oviposition. In present study, we revealed the distribution of chemosensilla in the ventral side of female fifth tarsomere in E. obliqua. To investigate its molecular mechanism of gustatory perception, we performed HiSeq 2500 sequencing of the male- and female- legs transcriptome and identified 24 candidate odorant binding proteins (OBPs), 21 chemosensory proteins (CSPs), 2 sensory neuron membrane proteins (SNMPs), 3 gustatory receptors (GRs) and 4 odorant receptors (ORs). Several leg-specific or enriched chemosensory genes were screened by tissue expression analysis, and clustered with functionally validated genes from other moths, suggesting the potential involvement in taste sensation or other physiological processes. The RPKM value analysis revealed that 9 EoblOBPs showed sex discrepancy in the leg expression, 8 being up-regulated in female and only 1 being over expressed in male. These female-biased EoblOBPs indicated an ecological adaption related with host-seeking and oviposition behaviors. Our work will provide basic knowledge for further studies on the molecular mechanism of gustatory perception, and enlighten a host-selection-based control strategy of insect pests.</description><subject>Agriculture</subject><subject>Agrotis ipsilon</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Butterflies & moths</subject><subject>Chemicals</subject><subject>Chemoreception</subject><subject>Chewing</subject><subject>Comparative studies</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Ectropis obliqua</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Ontology</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Helicoverpa</subject><subject>Helicoverpa armigera</subject><subject>Host plants</subject><subject>Host preferences</subject><subject>Host selection</subject><subject>Insect pests</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insects</subject><subject>Laboratories</subject><subject>Larvae</subject><subject>Leg</subject><subject>Legs</subject><subject>Lepidoptera</subject><subject>Ligands</subject><subject>Male</subject><subject>Mastication</subject><subject>Medicine and Health Sciences</subject><subject>Membrane proteins</subject><subject>Molecular Sequence Data</subject><subject>Moths - anatomy & histology</subject><subject>Moths - chemistry</subject><subject>Moths - genetics</subject><subject>Moths - physiology</subject><subject>Odorant receptors</subject><subject>Oviposition</subject><subject>Perception</subject><subject>Pests</subject><subject>Pheromones</subject><subject>Physiological aspects</subject><subject>Phytochemicals</subject><subject>Phytophagous insects</subject><subject>Plants - parasitology</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, Odorant - chemistry</subject><subject>Receptors, Odorant - genetics</subject><subject>Sequence Alignment</subject><subject>Social Sciences</subject><subject>Taste</subject><subject>Taste perception</subject><subject>Taste receptors</subject><subject>Tea</subject><subject>Tissue analysis</subject><subject>Transcriptome</subject><subject>Value analysis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9Fq2zAUhs3YWLtsbzA2wWBsF8kk2Zaim0IIXRsIFJqwW6FIx4mKbaWSXJZH2dtOadISj14Mg23pfOeX9OucLPtI8IjknPy4c51vVT3auhZGmBSiFORVdk5EToeM4vz1yf9Z9i6EO4zLfMzY2-yMMpGnETvP_swMtNFWVqtoXYtUa9DUNVvl0_gB0CJ2ZodchaYbaFyANji_Q1fQQkC3UKsIBkWHrl2IaAE16EeV1Q7NYR3Q0qs2aG-30TWAJmm7u2ADsi2KG0BLUEkpRaK3Bl3q6N02Rd2qtvedep-9qVQd4MPxO8iWPy-X0-vh_OZqNp3Mh5oJGofGKCEY5iVUotSrwhSMMAKaGKNZUamcljmIvOIaC2WSSxwwL8ZYUyLKFckH2eeD7LZ2QR5NDZJwJhjlgvJEzA6EcepObr1tlN9Jp6x8nHB-LZWPVtcgc25yYuhYUQyFYFrBCigtcVlxokoqktbFcbVu1YDRyXuv6p5oP9LajVy7B1lwnm6MJYFvRwHv7jsIUTY2aKhr1YLr9vvmmKYXoQn98g_68umO1FqlA9i2cmldvReVk6IoxrygqYAG2egFKj0GGqtTBVY2zfcSvvcSEhPhd1yrLgQ5W9z-P3vzq89-PWE3oOq4Ca7u9mUX-mBxALV3IXionk0mWO4b6MkNuW8geWyglPbp9IKek546Jv8LXrsXcg</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Ma, Long</creator><creator>Li, Zhao-Qun</creator><creator>Bian, Lei</creator><creator>Cai, Xiao-Ming</creator><creator>Luo, Zong-Xiu</creator><creator>Zhang, Yong-Jun</creator><creator>Chen, Zong-Mao</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160301</creationdate><title>Identification and Comparative Study of Chemosensory Genes Related to Host Selection by Legs Transcriptome Analysis in the Tea Geometrid Ectropis obliqua</title><author>Ma, Long ; Li, Zhao-Qun ; Bian, Lei ; Cai, Xiao-Ming ; Luo, Zong-Xiu ; Zhang, Yong-Jun ; Chen, Zong-Mao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-dda996075ef95cb4d46161ec1ddc64fa3253e93f7c09ad4957e07480c2195b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Agriculture</topic><topic>Agrotis ipsilon</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Butterflies & moths</topic><topic>Chemicals</topic><topic>Chemoreception</topic><topic>Chewing</topic><topic>Comparative studies</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Ectropis obliqua</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Ontology</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Helicoverpa</topic><topic>Helicoverpa armigera</topic><topic>Host plants</topic><topic>Host preferences</topic><topic>Host selection</topic><topic>Insect pests</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insects</topic><topic>Laboratories</topic><topic>Larvae</topic><topic>Leg</topic><topic>Legs</topic><topic>Lepidoptera</topic><topic>Ligands</topic><topic>Male</topic><topic>Mastication</topic><topic>Medicine and Health Sciences</topic><topic>Membrane proteins</topic><topic>Molecular Sequence Data</topic><topic>Moths - anatomy & histology</topic><topic>Moths - chemistry</topic><topic>Moths - genetics</topic><topic>Moths - physiology</topic><topic>Odorant receptors</topic><topic>Oviposition</topic><topic>Perception</topic><topic>Pests</topic><topic>Pheromones</topic><topic>Physiological aspects</topic><topic>Phytochemicals</topic><topic>Phytophagous insects</topic><topic>Plants - parasitology</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, Odorant - chemistry</topic><topic>Receptors, Odorant - genetics</topic><topic>Sequence Alignment</topic><topic>Social Sciences</topic><topic>Taste</topic><topic>Taste perception</topic><topic>Taste receptors</topic><topic>Tea</topic><topic>Tissue analysis</topic><topic>Transcriptome</topic><topic>Value analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Long</creatorcontrib><creatorcontrib>Li, Zhao-Qun</creatorcontrib><creatorcontrib>Bian, Lei</creatorcontrib><creatorcontrib>Cai, Xiao-Ming</creatorcontrib><creatorcontrib>Luo, Zong-Xiu</creatorcontrib><creatorcontrib>Zhang, Yong-Jun</creatorcontrib><creatorcontrib>Chen, Zong-Mao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Long</au><au>Li, Zhao-Qun</au><au>Bian, Lei</au><au>Cai, Xiao-Ming</au><au>Luo, Zong-Xiu</au><au>Zhang, Yong-Jun</au><au>Chen, Zong-Mao</au><au>Hull, J Joe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Comparative Study of Chemosensory Genes Related to Host Selection by Legs Transcriptome Analysis in the Tea Geometrid Ectropis obliqua</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0149591</spage><epage>e0149591</epage><pages>e0149591-e0149591</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Host selection by female moths is fundamental to the survival of their larvae. Detecting and perceiving the non-volatile chemicals of the plant surface involved in gustatory detection determine the host preference. In many lepidopteran species, tarsal chemosensilla are sensitive to non-volatile chemicals and responsible for taste detection. The tea geometrid Ectropis obliqua is one devastating chewing pest selectively feeding on limited plants, requiring the specialized sensors to forage certain host for oviposition. In present study, we revealed the distribution of chemosensilla in the ventral side of female fifth tarsomere in E. obliqua. To investigate its molecular mechanism of gustatory perception, we performed HiSeq 2500 sequencing of the male- and female- legs transcriptome and identified 24 candidate odorant binding proteins (OBPs), 21 chemosensory proteins (CSPs), 2 sensory neuron membrane proteins (SNMPs), 3 gustatory receptors (GRs) and 4 odorant receptors (ORs). Several leg-specific or enriched chemosensory genes were screened by tissue expression analysis, and clustered with functionally validated genes from other moths, suggesting the potential involvement in taste sensation or other physiological processes. The RPKM value analysis revealed that 9 EoblOBPs showed sex discrepancy in the leg expression, 8 being up-regulated in female and only 1 being over expressed in male. These female-biased EoblOBPs indicated an ecological adaption related with host-seeking and oviposition behaviors. Our work will provide basic knowledge for further studies on the molecular mechanism of gustatory perception, and enlighten a host-selection-based control strategy of insect pests.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26930056</pmid><doi>10.1371/journal.pone.0149591</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-03, Vol.11 (3), p.e0149591-e0149591 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1769627927 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agriculture Agrotis ipsilon Amino Acid Sequence Amino acids Animals Biology Biology and Life Sciences Butterflies & moths Chemicals Chemoreception Chewing Comparative studies Drosophila Drosophila melanogaster Ectropis obliqua Female Gene expression Gene Expression Profiling Gene Ontology Genes Genetic aspects Helicoverpa Helicoverpa armigera Host plants Host preferences Host selection Insect pests Insect Proteins - chemistry Insect Proteins - genetics Insects Laboratories Larvae Leg Legs Lepidoptera Ligands Male Mastication Medicine and Health Sciences Membrane proteins Molecular Sequence Data Moths - anatomy & histology Moths - chemistry Moths - genetics Moths - physiology Odorant receptors Oviposition Perception Pests Pheromones Physiological aspects Phytochemicals Phytophagous insects Plants - parasitology Proteins Receptors Receptors, Odorant - chemistry Receptors, Odorant - genetics Sequence Alignment Social Sciences Taste Taste perception Taste receptors Tea Tissue analysis Transcriptome Value analysis |
| title | Identification and Comparative Study of Chemosensory Genes Related to Host Selection by Legs Transcriptome Analysis in the Tea Geometrid Ectropis obliqua |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T17%3A57%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20Comparative%20Study%20of%20Chemosensory%20Genes%20Related%20to%20Host%20Selection%20by%20Legs%20Transcriptome%20Analysis%20in%20the%20Tea%20Geometrid%20Ectropis%20obliqua&rft.jtitle=PloS%20one&rft.au=Ma,%20Long&rft.date=2016-03-01&rft.volume=11&rft.issue=3&rft.spage=e0149591&rft.epage=e0149591&rft.pages=e0149591-e0149591&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0149591&rft_dat=%3Cgale_plos_%3EA444874220%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1769627927&rft_id=info:pmid/26930056&rft_galeid=A444874220&rft_doaj_id=oai_doaj_org_article_37d31d28a20e496caebe22505f71a529&rfr_iscdi=true |