Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model
Extended esophageal endoscopic submucosal dissection (ESD) is highly responsible for esophageal stricture. We conducted a comparative study in a porcine model to evaluate the effectiveness of adipose tissue-derived stromal cell (ADSC) double cell sheet transplantation. Twelve female pigs were treate...
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| creator | Perrod, Guillaume Rahmi, Gabriel Pidial, Laetitia Camilleri, Sophie Bellucci, Alexandre Casanova, Amaury Viel, Thomas Tavitian, Bertrand Cellier, Christophe Clement, Olivier |
| description | Extended esophageal endoscopic submucosal dissection (ESD) is highly responsible for esophageal stricture. We conducted a comparative study in a porcine model to evaluate the effectiveness of adipose tissue-derived stromal cell (ADSC) double cell sheet transplantation.
Twelve female pigs were treated with 5 cm long hemi-circumferential ESD and randomized in two groups. ADSC group (n = 6) received 4 double cell sheets of allogenic ADSC on a paper support membrane and control group (n = 6) received 4 paper support membranes. ADSC were labelled with PKH-67 fluorophore to allow probe-based confocal laser endomicroscopie (pCLE) monitoring. After 28 days follow-up, animals were sacrificed. At days 3, 14 and 28, endoscopic evaluation with pCLE and esophagography were performed.
One animal from the control group was excluded (anesthetic complication). Animals from ADSC group showed less frequent alimentary trouble (17% vs 80%; P = 0.08) and higher gain weight on day 28. pCLE demonstrated a compatible cell signal in 4 animals of the ADSC group at day 3. In ADSC group, endoscopy showed that 1 out of 6 (17%) animals developed a severe esophageal stricture comparatively to 100% (5/5) in the control group; P = 0.015. Esophagography demonstrated a decreased degree of stricture in the ADSC group on day 14 (44% vs 81%; P = 0.017) and day 28 (46% vs 90%; P = 0.035). Histological analysis showed a decreased fibrosis development in the ADSC group, in terms of surface (9.7 vs 26.1 mm²; P = 0.017) and maximal depth (1.6 vs 3.2 mm; P = 0.052).
In this model, transplantation of allogenic ADSC organized in double cell sheets after extended esophegeal ESD is strongly associated with a lower esophageal stricture's rate. |
| doi_str_mv | 10.1371/journal.pone.0148249 |
| format | Article |
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Twelve female pigs were treated with 5 cm long hemi-circumferential ESD and randomized in two groups. ADSC group (n = 6) received 4 double cell sheets of allogenic ADSC on a paper support membrane and control group (n = 6) received 4 paper support membranes. ADSC were labelled with PKH-67 fluorophore to allow probe-based confocal laser endomicroscopie (pCLE) monitoring. After 28 days follow-up, animals were sacrificed. At days 3, 14 and 28, endoscopic evaluation with pCLE and esophagography were performed.
One animal from the control group was excluded (anesthetic complication). Animals from ADSC group showed less frequent alimentary trouble (17% vs 80%; P = 0.08) and higher gain weight on day 28. pCLE demonstrated a compatible cell signal in 4 animals of the ADSC group at day 3. In ADSC group, endoscopy showed that 1 out of 6 (17%) animals developed a severe esophageal stricture comparatively to 100% (5/5) in the control group; P = 0.015. Esophagography demonstrated a decreased degree of stricture in the ADSC group on day 14 (44% vs 81%; P = 0.017) and day 28 (46% vs 90%; P = 0.035). Histological analysis showed a decreased fibrosis development in the ADSC group, in terms of surface (9.7 vs 26.1 mm²; P = 0.017) and maximal depth (1.6 vs 3.2 mm; P = 0.052).
In this model, transplantation of allogenic ADSC organized in double cell sheets after extended esophegeal ESD is strongly associated with a lower esophageal stricture's rate.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0148249</identifier><identifier>PMID: 26930409</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdominal Fat - pathology ; Adipose tissue ; Animals ; Biology and Life Sciences ; Care and treatment ; Cell Culture Techniques ; Cells, Cultured ; Comparative studies ; Cornea ; Dissection ; Endoscopes ; Endoscopy ; Endoscopy, Gastrointestinal - adverse effects ; Esophageal diseases ; Esophageal Neoplasms - surgery ; Esophageal Stenosis - etiology ; Esophageal Stenosis - prevention & control ; Esophagus ; Esophagus - diagnostic imaging ; Esophagus - pathology ; Female ; Fibrosis ; Gastroenterology ; Laboratories ; Medicine and Health Sciences ; Membranes ; Methods ; Pigs ; Postoperative Complications - prevention & control ; Prevention ; Radiography ; Research and Analysis Methods ; Stem cell transplantation ; Stem Cell Transplantation - methods ; Stem cells ; Stenosis ; Stricture ; Sus scrofa ; Swine ; Tissue engineering ; Transplantation ; Transplants & implants ; Ulcers ; Wound healing</subject><ispartof>PloS one, 2016-03, Vol.11 (3), p.e0148249-e0148249</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Perrod et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Perrod et al 2016 Perrod et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-28b72ddcbcc4450c4da478e441bf2621e3b26924d033ebff18d4a8aa99f5892b3</citedby><cites>FETCH-LOGICAL-c758t-28b72ddcbcc4450c4da478e441bf2621e3b26924d033ebff18d4a8aa99f5892b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773126/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773126/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26930409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sensebé, Luc</contributor><creatorcontrib>Perrod, Guillaume</creatorcontrib><creatorcontrib>Rahmi, Gabriel</creatorcontrib><creatorcontrib>Pidial, Laetitia</creatorcontrib><creatorcontrib>Camilleri, Sophie</creatorcontrib><creatorcontrib>Bellucci, Alexandre</creatorcontrib><creatorcontrib>Casanova, Amaury</creatorcontrib><creatorcontrib>Viel, Thomas</creatorcontrib><creatorcontrib>Tavitian, Bertrand</creatorcontrib><creatorcontrib>Cellier, Christophe</creatorcontrib><creatorcontrib>Clement, Olivier</creatorcontrib><title>Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Extended esophageal endoscopic submucosal dissection (ESD) is highly responsible for esophageal stricture. We conducted a comparative study in a porcine model to evaluate the effectiveness of adipose tissue-derived stromal cell (ADSC) double cell sheet transplantation.
Twelve female pigs were treated with 5 cm long hemi-circumferential ESD and randomized in two groups. ADSC group (n = 6) received 4 double cell sheets of allogenic ADSC on a paper support membrane and control group (n = 6) received 4 paper support membranes. ADSC were labelled with PKH-67 fluorophore to allow probe-based confocal laser endomicroscopie (pCLE) monitoring. After 28 days follow-up, animals were sacrificed. At days 3, 14 and 28, endoscopic evaluation with pCLE and esophagography were performed.
One animal from the control group was excluded (anesthetic complication). Animals from ADSC group showed less frequent alimentary trouble (17% vs 80%; P = 0.08) and higher gain weight on day 28. pCLE demonstrated a compatible cell signal in 4 animals of the ADSC group at day 3. In ADSC group, endoscopy showed that 1 out of 6 (17%) animals developed a severe esophageal stricture comparatively to 100% (5/5) in the control group; P = 0.015. Esophagography demonstrated a decreased degree of stricture in the ADSC group on day 14 (44% vs 81%; P = 0.017) and day 28 (46% vs 90%; P = 0.035). Histological analysis showed a decreased fibrosis development in the ADSC group, in terms of surface (9.7 vs 26.1 mm²; P = 0.017) and maximal depth (1.6 vs 3.2 mm; P = 0.052).
In this model, transplantation of allogenic ADSC organized in double cell sheets after extended esophegeal ESD is strongly associated with a lower esophageal stricture's rate.</description><subject>Abdominal Fat - pathology</subject><subject>Adipose tissue</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>Cell Culture Techniques</subject><subject>Cells, Cultured</subject><subject>Comparative studies</subject><subject>Cornea</subject><subject>Dissection</subject><subject>Endoscopes</subject><subject>Endoscopy</subject><subject>Endoscopy, Gastrointestinal - adverse effects</subject><subject>Esophageal diseases</subject><subject>Esophageal Neoplasms - surgery</subject><subject>Esophageal Stenosis - etiology</subject><subject>Esophageal Stenosis - prevention & control</subject><subject>Esophagus</subject><subject>Esophagus - diagnostic imaging</subject><subject>Esophagus - pathology</subject><subject>Female</subject><subject>Fibrosis</subject><subject>Gastroenterology</subject><subject>Laboratories</subject><subject>Medicine and Health Sciences</subject><subject>Membranes</subject><subject>Methods</subject><subject>Pigs</subject><subject>Postoperative Complications - prevention & control</subject><subject>Prevention</subject><subject>Radiography</subject><subject>Research and Analysis Methods</subject><subject>Stem cell transplantation</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Stenosis</subject><subject>Stricture</subject><subject>Sus scrofa</subject><subject>Swine</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><subject>Ulcers</subject><subject>Wound healing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKvJs4N0lQGVBraRAe31ok_WldpnNnONP497ppNDdoFykUi53lf-7w-J8teYzTFtMSfNq73LTTTzrV6ijDjhFVPsmNcUTIpCKJPD76PshchbBCaUV4Uz7MjUlQUMVQdZ7dz3TT5cq11zK88tKFroI0QrWtz43x-Fly3hpWGBEVvZey9zi-9vtHtHQMm6kS1ygXpOivzZV9ve-lCEnyxIWh5h9lE5pfOS9vq_IdTunmZPTPQBP1qeJ9kv76eXc2_T84vvi3mp-cTWc54nBBel0QpWUvJ2AxJpoCVXDOGa0MKgjWtUzGEKUSpro3BXDHgAFVlZrwiNT3J3u59u8YFMYQWBC6LqiCMVywRiz2hHGxE5-0W_B_hwIq7BedXAny0stFCKVCYoFnJasMwrcEUEhBwxDmqTQXJ6_OwW4pBK5lS8tCMTMd_WrsWK3cjWFlSTIpk8GEw8O661yGKrQ0y3RG02vW7c5eI4BQNTui7f9DHqxuoFaQCbGtc2lfuTMUpY4yXjOBZoqaPUOlRemtl6jBj0_pI8HEkSEzUt3EFfQhisfz5_-zF7zH7_oBdp76L6-CaftdFYQyyPSi9C8Fr8xAyRmI3IPdpiN2AiGFAkuzN4QU9iO4ngv4FH4INDg</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Perrod, Guillaume</creator><creator>Rahmi, Gabriel</creator><creator>Pidial, Laetitia</creator><creator>Camilleri, Sophie</creator><creator>Bellucci, Alexandre</creator><creator>Casanova, Amaury</creator><creator>Viel, Thomas</creator><creator>Tavitian, Bertrand</creator><creator>Cellier, Christophe</creator><creator>Clement, Olivier</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160301</creationdate><title>Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model</title><author>Perrod, Guillaume ; Rahmi, Gabriel ; Pidial, Laetitia ; Camilleri, Sophie ; Bellucci, Alexandre ; Casanova, Amaury ; Viel, Thomas ; Tavitian, Bertrand ; Cellier, Christophe ; Clement, Olivier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-28b72ddcbcc4450c4da478e441bf2621e3b26924d033ebff18d4a8aa99f5892b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Abdominal Fat - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perrod, Guillaume</au><au>Rahmi, Gabriel</au><au>Pidial, Laetitia</au><au>Camilleri, Sophie</au><au>Bellucci, Alexandre</au><au>Casanova, Amaury</au><au>Viel, Thomas</au><au>Tavitian, Bertrand</au><au>Cellier, Christophe</au><au>Clement, Olivier</au><au>Sensebé, Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>11</volume><issue>3</issue><spage>e0148249</spage><epage>e0148249</epage><pages>e0148249-e0148249</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Extended esophageal endoscopic submucosal dissection (ESD) is highly responsible for esophageal stricture. We conducted a comparative study in a porcine model to evaluate the effectiveness of adipose tissue-derived stromal cell (ADSC) double cell sheet transplantation.
Twelve female pigs were treated with 5 cm long hemi-circumferential ESD and randomized in two groups. ADSC group (n = 6) received 4 double cell sheets of allogenic ADSC on a paper support membrane and control group (n = 6) received 4 paper support membranes. ADSC were labelled with PKH-67 fluorophore to allow probe-based confocal laser endomicroscopie (pCLE) monitoring. After 28 days follow-up, animals were sacrificed. At days 3, 14 and 28, endoscopic evaluation with pCLE and esophagography were performed.
One animal from the control group was excluded (anesthetic complication). Animals from ADSC group showed less frequent alimentary trouble (17% vs 80%; P = 0.08) and higher gain weight on day 28. pCLE demonstrated a compatible cell signal in 4 animals of the ADSC group at day 3. In ADSC group, endoscopy showed that 1 out of 6 (17%) animals developed a severe esophageal stricture comparatively to 100% (5/5) in the control group; P = 0.015. Esophagography demonstrated a decreased degree of stricture in the ADSC group on day 14 (44% vs 81%; P = 0.017) and day 28 (46% vs 90%; P = 0.035). Histological analysis showed a decreased fibrosis development in the ADSC group, in terms of surface (9.7 vs 26.1 mm²; P = 0.017) and maximal depth (1.6 vs 3.2 mm; P = 0.052).
In this model, transplantation of allogenic ADSC organized in double cell sheets after extended esophegeal ESD is strongly associated with a lower esophageal stricture's rate.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26930409</pmid><doi>10.1371/journal.pone.0148249</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1769624894 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abdominal Fat - pathology Adipose tissue Animals Biology and Life Sciences Care and treatment Cell Culture Techniques Cells, Cultured Comparative studies Cornea Dissection Endoscopes Endoscopy Endoscopy, Gastrointestinal - adverse effects Esophageal diseases Esophageal Neoplasms - surgery Esophageal Stenosis - etiology Esophageal Stenosis - prevention & control Esophagus Esophagus - diagnostic imaging Esophagus - pathology Female Fibrosis Gastroenterology Laboratories Medicine and Health Sciences Membranes Methods Pigs Postoperative Complications - prevention & control Prevention Radiography Research and Analysis Methods Stem cell transplantation Stem Cell Transplantation - methods Stem cells Stenosis Stricture Sus scrofa Swine Tissue engineering Transplantation Transplants & implants Ulcers Wound healing |
| title | Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T08%3A34%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cell%20Sheet%20Transplantation%20for%20Esophageal%20Stricture%20Prevention%20after%20Endoscopic%20Submucosal%20Dissection%20in%20a%20Porcine%20Model&rft.jtitle=PloS%20one&rft.au=Perrod,%20Guillaume&rft.date=2016-03-01&rft.volume=11&rft.issue=3&rft.spage=e0148249&rft.epage=e0148249&rft.pages=e0148249-e0148249&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0148249&rft_dat=%3Cgale_plos_%3EA444874215%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1769624894&rft_id=info:pmid/26930409&rft_galeid=A444874215&rft_doaj_id=oai_doaj_org_article_ddad120574bf413baf6ca0a80880bf9a&rfr_iscdi=true |