Clonal Strain Persistence of Candida albicans Isolates from Chronic Mucocutaneous Candidiasis Patients
Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not...
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| description | Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST). Multiple colonies were typed from swabs taken from multiple body sites across multiple time points over a 17-month period. Among isolates from each individual patient, our data show clonal and persistent diploid sequence types (DSTs) that were stable over time, identical between multiple infection sites and exhibit azole resistant phenotypes. No shared origin or common source of infection was identified among isolates from these patients. Additionally, we performed C. albicans MLST SNP genotype frequency analysis to identify signatures of past loss of heterozygosity (LOH) events among persistent and azole resistant isolates retrieved from patients with autoimmune disorders including CMC. |
| doi_str_mv | 10.1371/journal.pone.0145888 |
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Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST). Multiple colonies were typed from swabs taken from multiple body sites across multiple time points over a 17-month period. Among isolates from each individual patient, our data show clonal and persistent diploid sequence types (DSTs) that were stable over time, identical between multiple infection sites and exhibit azole resistant phenotypes. No shared origin or common source of infection was identified among isolates from these patients. Additionally, we performed C. albicans MLST SNP genotype frequency analysis to identify signatures of past loss of heterozygosity (LOH) events among persistent and azole resistant isolates retrieved from patients with autoimmune disorders including CMC.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0145888</identifier><identifier>PMID: 26849050</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Analysis ; Antifungal agents ; Antifungal Agents - pharmacology ; Autoimmune diseases ; Biology and Life Sciences ; Candida ; Candida albicans ; Candida albicans - classification ; Candida albicans - drug effects ; Candida albicans - genetics ; Candida albicans - isolation & purification ; Candidiasis ; Candidiasis, Chronic Mucocutaneous - microbiology ; Causes of ; Chronic Disease ; Chronic mucocutaneous candidiasis ; Colonies ; Datasets as Topic ; Epidemiology ; Female ; Frequency analysis ; Fungi ; Genetic aspects ; Genetic variation ; Genetics ; Health aspects ; Heterozygosity ; Humans ; Immunodeficiency ; Infections ; Loss of heterozygosity ; Male ; Medicine and Health Sciences ; Membranes ; Microbial Sensitivity Tests ; Middle Aged ; Multilocus Sequence Typing ; Mutation ; Nails (Anatomy) ; Nosocomial infections ; Patients ; Phylogeny ; Physical Sciences ; Physiological aspects ; Polymorphism, Single Nucleotide ; Population ; Primary immunodeficiencies ; Research and Analysis Methods ; Skin ; Studies ; Transcription factors</subject><ispartof>PloS one, 2016-02, Vol.11 (2), p.e0145888-e0145888</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Moorhouse et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST). Multiple colonies were typed from swabs taken from multiple body sites across multiple time points over a 17-month period. Among isolates from each individual patient, our data show clonal and persistent diploid sequence types (DSTs) that were stable over time, identical between multiple infection sites and exhibit azole resistant phenotypes. No shared origin or common source of infection was identified among isolates from these patients. 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titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moorhouse, Alexander J</au><au>Rennison, Claire</au><au>Raza, Muhammad</au><au>Lilic, Desa</au><au>Gow, Neil A R</au><au>Jacobsen, Ilse D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonal Strain Persistence of Candida albicans Isolates from Chronic Mucocutaneous Candidiasis Patients</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-02-05</date><risdate>2016</risdate><volume>11</volume><issue>2</issue><spage>e0145888</spage><epage>e0145888</epage><pages>e0145888-e0145888</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST). Multiple colonies were typed from swabs taken from multiple body sites across multiple time points over a 17-month period. Among isolates from each individual patient, our data show clonal and persistent diploid sequence types (DSTs) that were stable over time, identical between multiple infection sites and exhibit azole resistant phenotypes. No shared origin or common source of infection was identified among isolates from these patients. Additionally, we performed C. albicans MLST SNP genotype frequency analysis to identify signatures of past loss of heterozygosity (LOH) events among persistent and azole resistant isolates retrieved from patients with autoimmune disorders including CMC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26849050</pmid><doi>10.1371/journal.pone.0145888</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Analysis Antifungal agents Antifungal Agents - pharmacology Autoimmune diseases Biology and Life Sciences Candida Candida albicans Candida albicans - classification Candida albicans - drug effects Candida albicans - genetics Candida albicans - isolation & purification Candidiasis Candidiasis, Chronic Mucocutaneous - microbiology Causes of Chronic Disease Chronic mucocutaneous candidiasis Colonies Datasets as Topic Epidemiology Female Frequency analysis Fungi Genetic aspects Genetic variation Genetics Health aspects Heterozygosity Humans Immunodeficiency Infections Loss of heterozygosity Male Medicine and Health Sciences Membranes Microbial Sensitivity Tests Middle Aged Multilocus Sequence Typing Mutation Nails (Anatomy) Nosocomial infections Patients Phylogeny Physical Sciences Physiological aspects Polymorphism, Single Nucleotide Population Primary immunodeficiencies Research and Analysis Methods Skin Studies Transcription factors |
| title | Clonal Strain Persistence of Candida albicans Isolates from Chronic Mucocutaneous Candidiasis Patients |
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