Evaluating the Impact of Breastfeeding on Rotavirus Antigenemia and Disease Severity in Indian Children
To evaluate the contribution of breastfeeding to Rotavirus (RV)-induced antigenemia and/or RNAemia and disease severity in Indian children (
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| Veröffentlicht in: | PloS one 2016-02, Vol.11 (2), p.e0146243-e0146243 |
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| creator | Das, Sushmita Sahoo, Ganesh Chandra Das, Pradeep Singh, Utpal Kant Jaiswal, Anil Kumar Singh, Prachi Kumar, Ranjeet Kumar, Rishikesh |
| description | To evaluate the contribution of breastfeeding to Rotavirus (RV)-induced antigenemia and/or RNAemia and disease severity in Indian children ( |
| doi_str_mv | 10.1371/journal.pone.0146243 |
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Paired stool and serum samples were collected from (a) hospitalized infants with diarrhea (n = 145) and (b) healthy control infants without diarrhea (n = 28). Stool RV-antigen was screened in both groups by commercial rapid-test and enzyme immunoassay. The disease severity was scored and real-time-PCR was used for viral-load estimation. Serum was evaluated for RV-antigenemia by EIA and RV-RNAemia by RT-PCR. Data was stratified by age-group and breastfeeding status and compared.
Presence of RV-antigenemia and RV-RNAemia was positively related with presence of RV in stool. Disease severity and stool viral-load was significantly associated with RV-antigenemia [(r = 0.74; CI:0.66 to 0.84; P<0.0001,R2 = 0.59) and (r = -0.55; CI:-0.68 to -0.39; P<0.0001,R2 = 0.31) respectively], but not with RV-RNAemia. There was significant reduction in RV-antigenemiarate in the breast-fed group compared to non-breastfed infants, especially in 0-6 month age group (P<0.001). Non-breastfed infants were at risk for RV-antigenemia with severe disease manifestations in form of high Vesikari scores correlating with high fever, more vomiting episodes and dehydration.
RV-antigenemia was common in nonbreastfed children with severe RV-diarrhea and correlated with stool RV-load and disease severity.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0146243</identifier><identifier>PMID: 26828823</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Antigenemia ; Antigens ; Antigens, Viral - blood ; Biology and life sciences ; Breast Feeding ; Breastfeeding & lactation ; Child ; Children ; Children & youth ; Complications and side effects ; Correlation analysis ; Dehydration ; Demographic aspects ; Diarrhea ; Enzyme immunoassay ; Families & family life ; Female ; Fever ; Health aspects ; Health risks ; Hospitalization ; Humans ; Immunoassay ; Immunoglobulins ; India - epidemiology ; Infants ; Infections ; Low income groups ; Medicine and Health Sciences ; People and Places ; Polymerase chain reaction ; RNA, Viral - blood ; Rotavirus ; Rotavirus - immunology ; Rotavirus infections ; Severity of Illness Index ; Vaccines ; Viral Load ; Viremia - blood ; Viremia - epidemiology ; Virology ; Viruses ; Vomiting</subject><ispartof>PloS one, 2016-02, Vol.11 (2), p.e0146243-e0146243</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Das et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Das et al 2016 Das et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-5293658af5c0cefcd90c8b41f559a864cb8fef1d0cfab8a9437193e82022286b3</citedby><cites>FETCH-LOGICAL-c692t-5293658af5c0cefcd90c8b41f559a864cb8fef1d0cfab8a9437193e82022286b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734603/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734603/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26828823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Jhaveri, Ravi</contributor><creatorcontrib>Das, Sushmita</creatorcontrib><creatorcontrib>Sahoo, Ganesh Chandra</creatorcontrib><creatorcontrib>Das, Pradeep</creatorcontrib><creatorcontrib>Singh, Utpal Kant</creatorcontrib><creatorcontrib>Jaiswal, Anil Kumar</creatorcontrib><creatorcontrib>Singh, Prachi</creatorcontrib><creatorcontrib>Kumar, Ranjeet</creatorcontrib><creatorcontrib>Kumar, Rishikesh</creatorcontrib><title>Evaluating the Impact of Breastfeeding on Rotavirus Antigenemia and Disease Severity in Indian Children</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>To evaluate the contribution of breastfeeding to Rotavirus (RV)-induced antigenemia and/or RNAemia and disease severity in Indian children (<2 yrs age).
Paired stool and serum samples were collected from (a) hospitalized infants with diarrhea (n = 145) and (b) healthy control infants without diarrhea (n = 28). Stool RV-antigen was screened in both groups by commercial rapid-test and enzyme immunoassay. The disease severity was scored and real-time-PCR was used for viral-load estimation. Serum was evaluated for RV-antigenemia by EIA and RV-RNAemia by RT-PCR. Data was stratified by age-group and breastfeeding status and compared.
Presence of RV-antigenemia and RV-RNAemia was positively related with presence of RV in stool. Disease severity and stool viral-load was significantly associated with RV-antigenemia [(r = 0.74; CI:0.66 to 0.84; P<0.0001,R2 = 0.59) and (r = -0.55; CI:-0.68 to -0.39; P<0.0001,R2 = 0.31) respectively], but not with RV-RNAemia. There was significant reduction in RV-antigenemiarate in the breast-fed group compared to non-breastfed infants, especially in 0-6 month age group (P<0.001). Non-breastfed infants were at risk for RV-antigenemia with severe disease manifestations in form of high Vesikari scores correlating with high fever, more vomiting episodes and dehydration.
RV-antigenemia was common in nonbreastfed children with severe RV-diarrhea and correlated with stool RV-load and disease severity.</description><subject>Age</subject><subject>Antigenemia</subject><subject>Antigens</subject><subject>Antigens, Viral - blood</subject><subject>Biology and life sciences</subject><subject>Breast Feeding</subject><subject>Breastfeeding & lactation</subject><subject>Child</subject><subject>Children</subject><subject>Children & youth</subject><subject>Complications and side effects</subject><subject>Correlation analysis</subject><subject>Dehydration</subject><subject>Demographic aspects</subject><subject>Diarrhea</subject><subject>Enzyme immunoassay</subject><subject>Families & family life</subject><subject>Female</subject><subject>Fever</subject><subject>Health aspects</subject><subject>Health risks</subject><subject>Hospitalization</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoglobulins</subject><subject>India - epidemiology</subject><subject>Infants</subject><subject>Infections</subject><subject>Low income groups</subject><subject>Medicine and Health Sciences</subject><subject>People and Places</subject><subject>Polymerase chain reaction</subject><subject>RNA, Viral - blood</subject><subject>Rotavirus</subject><subject>Rotavirus - immunology</subject><subject>Rotavirus infections</subject><subject>Severity of Illness Index</subject><subject>Vaccines</subject><subject>Viral Load</subject><subject>Viremia - blood</subject><subject>Viremia - epidemiology</subject><subject>Virology</subject><subject>Viruses</subject><subject>Vomiting</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01vEzEQhlcIREvhHyBYCQnBIcFf63gvSCEUiFSpUgtcLa93vHG0sVPbG9F_j9OkVYJ6QD7YmnnmHc_YUxSvMRpjOsGfln4ITvXjtXcwRphxwuiT4hTXlIw4QfTpwfmkeBHjEqGKCs6fFyeECyIEoadFd75R_aCSdV2ZFlDOV2ulU-lN-SWAiskAtFufd-WVT2pjwxDLqUu2Awcrq0rl2vKrjZmF8ho2EGy6La0r5661ypWzhe3bAO5l8cyoPsKr_X5W_Pp2_nP2Y3Rx-X0-m16MNK9JGlWkprwSylQaaTC6rZEWDcOmqmolONONMGBwi7RRjVA1y52oKQiCCCGCN_SseLvTXfc-yn2PosQTjjknNaozMd8RrVdLuQ52pcKt9MrKO4MPnVQhWd2DnCjaAKpyflaz1lSqNprQBmMmasaxyVqf99mGZgWtBpeC6o9Ejz3OLmTnN5JNKOOIZoEPe4HgbwaISa5s1ND3yoEf7u5NMkkrltF3_6CPV7enOpULsM74nFdvReWUMYLQpBJbavwIlVeb31TnD2Vsth8FfDwKyEyCP6lTQ4xyfn31_-zl72P2_QG7ANWnRfT9kKx38RhkO1AHH2MA89BkjOR2Hu67IbfzIPfzkMPeHD7QQ9D9ANC_QqUFKw</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Das, Sushmita</creator><creator>Sahoo, Ganesh Chandra</creator><creator>Das, Pradeep</creator><creator>Singh, Utpal Kant</creator><creator>Jaiswal, Anil Kumar</creator><creator>Singh, Prachi</creator><creator>Kumar, Ranjeet</creator><creator>Kumar, Rishikesh</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160201</creationdate><title>Evaluating the Impact of Breastfeeding on Rotavirus Antigenemia and Disease Severity in Indian Children</title><author>Das, Sushmita ; Sahoo, Ganesh Chandra ; Das, Pradeep ; Singh, Utpal Kant ; Jaiswal, Anil Kumar ; Singh, Prachi ; Kumar, Ranjeet ; Kumar, Rishikesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-5293658af5c0cefcd90c8b41f559a864cb8fef1d0cfab8a9437193e82022286b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Age</topic><topic>Antigenemia</topic><topic>Antigens</topic><topic>Antigens, Viral - blood</topic><topic>Biology and life sciences</topic><topic>Breast Feeding</topic><topic>Breastfeeding & lactation</topic><topic>Child</topic><topic>Children</topic><topic>Children & youth</topic><topic>Complications and side effects</topic><topic>Correlation analysis</topic><topic>Dehydration</topic><topic>Demographic aspects</topic><topic>Diarrhea</topic><topic>Enzyme immunoassay</topic><topic>Families & family life</topic><topic>Female</topic><topic>Fever</topic><topic>Health aspects</topic><topic>Health risks</topic><topic>Hospitalization</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Immunoglobulins</topic><topic>India - epidemiology</topic><topic>Infants</topic><topic>Infections</topic><topic>Low income groups</topic><topic>Medicine and Health Sciences</topic><topic>People and Places</topic><topic>Polymerase chain reaction</topic><topic>RNA, Viral - blood</topic><topic>Rotavirus</topic><topic>Rotavirus - immunology</topic><topic>Rotavirus infections</topic><topic>Severity of Illness Index</topic><topic>Vaccines</topic><topic>Viral Load</topic><topic>Viremia - blood</topic><topic>Viremia - epidemiology</topic><topic>Virology</topic><topic>Viruses</topic><topic>Vomiting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Sushmita</creatorcontrib><creatorcontrib>Sahoo, Ganesh Chandra</creatorcontrib><creatorcontrib>Das, Pradeep</creatorcontrib><creatorcontrib>Singh, Utpal Kant</creatorcontrib><creatorcontrib>Jaiswal, Anil Kumar</creatorcontrib><creatorcontrib>Singh, Prachi</creatorcontrib><creatorcontrib>Kumar, Ranjeet</creatorcontrib><creatorcontrib>Kumar, Rishikesh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Sushmita</au><au>Sahoo, Ganesh Chandra</au><au>Das, Pradeep</au><au>Singh, Utpal Kant</au><au>Jaiswal, Anil Kumar</au><au>Singh, Prachi</au><au>Kumar, Ranjeet</au><au>Kumar, Rishikesh</au><au>Jhaveri, Ravi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluating the Impact of Breastfeeding on Rotavirus Antigenemia and Disease Severity in Indian Children</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>11</volume><issue>2</issue><spage>e0146243</spage><epage>e0146243</epage><pages>e0146243-e0146243</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To evaluate the contribution of breastfeeding to Rotavirus (RV)-induced antigenemia and/or RNAemia and disease severity in Indian children (<2 yrs age).
Paired stool and serum samples were collected from (a) hospitalized infants with diarrhea (n = 145) and (b) healthy control infants without diarrhea (n = 28). Stool RV-antigen was screened in both groups by commercial rapid-test and enzyme immunoassay. The disease severity was scored and real-time-PCR was used for viral-load estimation. Serum was evaluated for RV-antigenemia by EIA and RV-RNAemia by RT-PCR. Data was stratified by age-group and breastfeeding status and compared.
Presence of RV-antigenemia and RV-RNAemia was positively related with presence of RV in stool. Disease severity and stool viral-load was significantly associated with RV-antigenemia [(r = 0.74; CI:0.66 to 0.84; P<0.0001,R2 = 0.59) and (r = -0.55; CI:-0.68 to -0.39; P<0.0001,R2 = 0.31) respectively], but not with RV-RNAemia. There was significant reduction in RV-antigenemiarate in the breast-fed group compared to non-breastfed infants, especially in 0-6 month age group (P<0.001). Non-breastfed infants were at risk for RV-antigenemia with severe disease manifestations in form of high Vesikari scores correlating with high fever, more vomiting episodes and dehydration.
RV-antigenemia was common in nonbreastfed children with severe RV-diarrhea and correlated with stool RV-load and disease severity.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26828823</pmid><doi>10.1371/journal.pone.0146243</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Age Antigenemia Antigens Antigens, Viral - blood Biology and life sciences Breast Feeding Breastfeeding & lactation Child Children Children & youth Complications and side effects Correlation analysis Dehydration Demographic aspects Diarrhea Enzyme immunoassay Families & family life Female Fever Health aspects Health risks Hospitalization Humans Immunoassay Immunoglobulins India - epidemiology Infants Infections Low income groups Medicine and Health Sciences People and Places Polymerase chain reaction RNA, Viral - blood Rotavirus Rotavirus - immunology Rotavirus infections Severity of Illness Index Vaccines Viral Load Viremia - blood Viremia - epidemiology Virology Viruses Vomiting |
| title | Evaluating the Impact of Breastfeeding on Rotavirus Antigenemia and Disease Severity in Indian Children |
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