Decreased Expression of a Gene Caused by a T-DNA Insertion in an Adjacent Gene in Arabidopsis
ALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutati...
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| description | ALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a T-DNA insertion in one gene can have effects on the expression of neighboring genes. |
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An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a T-DNA insertion in one gene can have effects on the expression of neighboring genes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0147911</identifier><identifier>PMID: 26828726</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Arabidopsis ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biology and Life Sciences ; Chlorophyll ; Chloroplast Proteins - metabolism ; Chloroplasts ; Chloroplasts - ultrastructure ; Cloning ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - genetics ; Efficiency ; Eukaryotes ; Gene expression ; Gene Expression Regulation, Plant ; Gene Knockout Techniques ; Genes ; Genes, Plant ; Genetic aspects ; Genomes ; Insertion ; miRNA ; Mutagenesis ; Mutagenesis, Insertional - genetics ; Mutation ; Mutation - genetics ; Nuclear Pore Complex Proteins - genetics ; Nuclear Pore Complex Proteins - metabolism ; Oryza ; Phenotype ; Photosynthesis ; Photosynthesis - genetics ; Physical Sciences ; Physiological aspects ; Plant growth ; Protein Biosynthesis - genetics ; Proteins ; Research and Analysis Methods ; Ribonucleic acid ; Ribosomal Proteins - genetics ; Ribosomal Proteins - metabolism ; Ribulose-bisphosphate carboxylase ; RNA ; T-DNA ; Transmission electron microscopy</subject><ispartof>PloS one, 2016-02, Vol.11 (2), p.e0147911</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Tamura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Tamura et al 2016 Tamura et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c769t-20d688d7842a27742b7d6b58830d470c2580ffd2d3f6920553e85fd1726f31483</citedby><cites>FETCH-LOGICAL-c769t-20d688d7842a27742b7d6b58830d470c2580ffd2d3f6920553e85fd1726f31483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734701/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734701/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26828726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Uemura, Matsuo</contributor><creatorcontrib>Tamura, Kentaro</creatorcontrib><creatorcontrib>Kawabayashi, Takenori</creatorcontrib><creatorcontrib>Shikanai, Toshiharu</creatorcontrib><creatorcontrib>Hara-Nishimura, Ikuko</creatorcontrib><title>Decreased Expression of a Gene Caused by a T-DNA Insertion in an Adjacent Gene in Arabidopsis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>ALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a T-DNA insertion in one gene can have effects on the expression of neighboring genes.</description><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biology and Life Sciences</subject><subject>Chlorophyll</subject><subject>Chloroplast Proteins - metabolism</subject><subject>Chloroplasts</subject><subject>Chloroplasts - ultrastructure</subject><subject>Cloning</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Efficiency</subject><subject>Eukaryotes</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockout Techniques</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Insertion</subject><subject>miRNA</subject><subject>Mutagenesis</subject><subject>Mutagenesis, Insertional - genetics</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Nuclear Pore Complex Proteins - genetics</subject><subject>Nuclear Pore Complex Proteins - metabolism</subject><subject>Oryza</subject><subject>Phenotype</subject><subject>Photosynthesis</subject><subject>Photosynthesis - genetics</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Plant growth</subject><subject>Protein Biosynthesis - genetics</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Ribonucleic acid</subject><subject>Ribosomal Proteins - genetics</subject><subject>Ribosomal Proteins - metabolism</subject><subject>Ribulose-bisphosphate carboxylase</subject><subject>RNA</subject><subject>T-DNA</subject><subject>Transmission electron microscopy</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguBFx3w1SW-EMruuA4sLunonIc3HTIZOMyat7P57U6e7TEFBcpFw8pz35Jy8WfYSggXEDL7f-iF0sl3sfWcWABJWQfgoO4UVRgVFAD8-Op9kz2LcAlBiTunT7ARRjjhD9DT7cW5UMDIanV_c7oOJ0fku9zaX-aXpTL6Uw3jX3KXATXH-uc5XXTShHynX5bLLa72VynT9gU-xOsjGab-PLj7PnljZRvNi2s-ybx8vbpafiqvry9WyvioUo1VfIKAp55pxgiRijKCGadqUnGOgCQMKlRxYq5HGllYIlCU2vLQapg4shoTjs-z1QXff-iimyUQBGYWUAgpGYnUgtJdbsQ9uJ8Od8NKJPwEf1kKmrlRrRJVKVZSm2qUlqCRNBSVLxYii3GpeJa0PU7Wh2Rk9Nh9kOxOd33RuI9b-lyAMp3ZgEngzCQT_czCx_8eTJ2ot06tcZ30SUzsXlagJQQCwEoNELf5CpaXNzqnkDetSfJbwbpaQmN7c9uv00VGsvn75f_b6-5x9e8RujGz7TfTtMFolzkFyAFXwMQZjHyYHgRitfT8NMVpbTNZOaa-Op_6QdO9l_BvMJ_Ah</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Tamura, Kentaro</creator><creator>Kawabayashi, Takenori</creator><creator>Shikanai, Toshiharu</creator><creator>Hara-Nishimura, Ikuko</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160201</creationdate><title>Decreased Expression of a Gene Caused by a T-DNA Insertion in an Adjacent Gene in Arabidopsis</title><author>Tamura, Kentaro ; Kawabayashi, Takenori ; Shikanai, Toshiharu ; Hara-Nishimura, Ikuko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c769t-20d688d7842a27742b7d6b58830d470c2580ffd2d3f6920553e85fd1726f31483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biology and Life Sciences</topic><topic>Chlorophyll</topic><topic>Chloroplast Proteins - metabolism</topic><topic>Chloroplasts</topic><topic>Chloroplasts - ultrastructure</topic><topic>Cloning</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Efficiency</topic><topic>Eukaryotes</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Knockout Techniques</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Insertion</topic><topic>miRNA</topic><topic>Mutagenesis</topic><topic>Mutagenesis, Insertional - genetics</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Nuclear Pore Complex Proteins - genetics</topic><topic>Nuclear Pore Complex Proteins - metabolism</topic><topic>Oryza</topic><topic>Phenotype</topic><topic>Photosynthesis</topic><topic>Photosynthesis - 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An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a T-DNA insertion in one gene can have effects on the expression of neighboring genes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26828726</pmid><doi>10.1371/journal.pone.0147911</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis Arabidopsis - genetics Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biology and Life Sciences Chlorophyll Chloroplast Proteins - metabolism Chloroplasts Chloroplasts - ultrastructure Cloning Deoxyribonucleic acid DNA DNA, Bacterial - genetics Efficiency Eukaryotes Gene expression Gene Expression Regulation, Plant Gene Knockout Techniques Genes Genes, Plant Genetic aspects Genomes Insertion miRNA Mutagenesis Mutagenesis, Insertional - genetics Mutation Mutation - genetics Nuclear Pore Complex Proteins - genetics Nuclear Pore Complex Proteins - metabolism Oryza Phenotype Photosynthesis Photosynthesis - genetics Physical Sciences Physiological aspects Plant growth Protein Biosynthesis - genetics Proteins Research and Analysis Methods Ribonucleic acid Ribosomal Proteins - genetics Ribosomal Proteins - metabolism Ribulose-bisphosphate carboxylase RNA T-DNA Transmission electron microscopy |
| title | Decreased Expression of a Gene Caused by a T-DNA Insertion in an Adjacent Gene in Arabidopsis |
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