Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase

The enzyme dihydrodipicolinate reductase (DHDPR) is a component of the lysine biosynthetic pathway in bacteria and higher plants. DHDPR catalyzes the NAD(P)H dependent reduction of 2,3-dihydrodipicolinate to the cyclic imine L-2,3,4,5,-tetrahydropicolinic acid. The dapB gene that encodes dihydrodipi...

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Veröffentlicht in:	PloS one 2016-01, Vol.11 (1), p.e0146525-e0146525
Hauptverfasser:	Trigoso, Yvonne D, Evans, Russell C, Karsten, William E, Chooback, Lilian
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Analysis Bacteria Biochemistry Biology and Life Sciences Biosynthesis Cell culture Chromatography Chromatography, Affinity Cloning Cloning, Molecular Deoxyribonucleic acid Dihydrodipicolinate reductase Dihydrodipicolinate Reductase - chemistry Dihydrodipicolinate Reductase - genetics Dihydrodipicolinate Reductase - metabolism DNA E coli Electrophoresis, Polyacrylamide Gel Endonuclease Enzyme kinetics Enzymes Escherichia coli Escherichia coli - enzymology Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gel electrophoresis Gene expression Genetic aspects Histidine Histidine - genetics Histidine - metabolism Lysine Medicine and Health Sciences Molecular weight Mycobacterium tuberculosis NAD Oligonucleotides Oligopeptides - genetics Oligopeptides - metabolism Physical Sciences Physiological aspects Plants (botany) Plasmids Plasmids - genetics Plasmids - metabolism Polymerase chain reaction Primers Production processes Protein expression Protein purification Proteins Purification Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Research and Analysis Methods Sodium lauryl sulfate Staphylococcus aureus Staphylococcus infections Transformed cells Tuberculosis Vectors (Biology)
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