Translational control of UIS4 protein of the host-parasite interface is mediated by the RNA binding protein Puf2 in Plasmodium berghei sporozoites
Copyright: © 2016 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. UIS4 is a key protein component o...
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| description | Copyright: © 2016 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UIS4 is a key protein component of the host-parasite interface in the liver stage of the rodent malaria parasite Plasmodium berghei and required for parasite survival after invasion. In the infectious sporozoite, UIS4 protein has variably been shown to be translated but also been reported to be translationally repressed. Here we show that uis4 mRNA translation is regulated by the P. berghei RNA binding protein Pumilio-2 (PbPuf2 or Puf2 from here on forward) in infectious salivary gland sporozoites in the mosquito vector. Using RNA immunoprecipitation we show that uis4 mRNA is bound by Puf2 in salivary gland sporozoites. In the absence of Puf2, uis4 mRNA translation is de-regulated and UIS4 protein expression upregulated in salivary gland sporozoites. Here, using RNA immunoprecipitation, we reveal the first Puf2-regulated mRNA in this parasite.
This work was supported by Fundação para a Ciência e a Tecnologia (FCT) grants PTDC/SAU-MIC/122082/2010 and PTDC/BIA-BCM/105610/2008 to GRM, and SFRH/BPD/72619/2010 to PAGCS. |
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UIS4 is a key protein component of the host-parasite interface in the liver stage of the rodent malaria parasite Plasmodium berghei and required for parasite survival after invasion. In the infectious sporozoite, UIS4 protein has variably been shown to be translated but also been reported to be translationally repressed. Here we show that uis4 mRNA translation is regulated by the P. berghei RNA binding protein Pumilio-2 (PbPuf2 or Puf2 from here on forward) in infectious salivary gland sporozoites in the mosquito vector. Using RNA immunoprecipitation we show that uis4 mRNA is bound by Puf2 in salivary gland sporozoites. In the absence of Puf2, uis4 mRNA translation is de-regulated and UIS4 protein expression upregulated in salivary gland sporozoites. Here, using RNA immunoprecipitation, we reveal the first Puf2-regulated mRNA in this parasite.
This work was supported by Fundação para a Ciência e a Tecnologia (FCT) grants PTDC/SAU-MIC/122082/2010 and PTDC/BIA-BCM/105610/2008 to GRM, and SFRH/BPD/72619/2010 to PAGCS.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0147940</identifier><identifier>PMID: 26808677</identifier><language>eng</language><publisher>United States: PLoS</publisher><subject>Animals ; Aquatic insects ; Binding proteins ; Biology and Life Sciences ; Causes of ; Chemical properties ; Drosophila ; Gene expression ; Gene Expression Regulation ; Genes ; Genetic aspects ; Genetic regulation ; Host-Parasite Interactions ; Immunoprecipitation ; Infectious diseases ; Insects ; Laboratory animals ; Liver ; Malaria ; Medicine and Health Sciences ; Mosquitoes ; mRNA ; Parasites ; Parasitology ; Physiological aspects ; Plasmodium (Protozoa) ; Plasmodium berghei ; Plasmodium berghei - metabolism ; Protein expression ; Proteins ; Protozoan Proteins - genetics ; Protozoan Proteins - metabolism ; Ribonucleic acid ; RNA ; RNA-binding protein ; RNA-Binding Proteins - metabolism ; Salivary gland ; Salivary Glands - metabolism ; Sporozoites ; Sporozoites - metabolism ; Translation ; Trends ; Vector-borne diseases</subject><ispartof>PloS one, 2016-01, Vol.11 (1), p.e0147940-e0147940</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Silva et al 2016 Silva et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c784t-7a86240a386843d8b06b9528991f921a02cde9168fb3e04fe6d103981e9ab19b3</citedby><cites>FETCH-LOGICAL-c784t-7a86240a386843d8b06b9528991f921a02cde9168fb3e04fe6d103981e9ab19b3</cites><orcidid>0000-0002-4940-5207 ; 0000-0002-5187-3691 ; 0000-0002-2173-6514</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726560/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726560/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26808677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Snounou, Georges</contributor><creatorcontrib>Silva, Patrícia A. G. C.</creatorcontrib><creatorcontrib>dos Santos Guerreiro, Ana Maria</creatorcontrib><creatorcontrib>Santos, Jorge</creatorcontrib><creatorcontrib>Braks, Joanna A. M.</creatorcontrib><creatorcontrib>Janse, Chris J.</creatorcontrib><creatorcontrib>Mair, Gunnar</creatorcontrib><title>Translational control of UIS4 protein of the host-parasite interface is mediated by the RNA binding protein Puf2 in Plasmodium berghei sporozoites</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Copyright: © 2016 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UIS4 is a key protein component of the host-parasite interface in the liver stage of the rodent malaria parasite Plasmodium berghei and required for parasite survival after invasion. In the infectious sporozoite, UIS4 protein has variably been shown to be translated but also been reported to be translationally repressed. Here we show that uis4 mRNA translation is regulated by the P. berghei RNA binding protein Pumilio-2 (PbPuf2 or Puf2 from here on forward) in infectious salivary gland sporozoites in the mosquito vector. Using RNA immunoprecipitation we show that uis4 mRNA is bound by Puf2 in salivary gland sporozoites. In the absence of Puf2, uis4 mRNA translation is de-regulated and UIS4 protein expression upregulated in salivary gland sporozoites. Here, using RNA immunoprecipitation, we reveal the first Puf2-regulated mRNA in this parasite.
This work was supported by Fundação para a Ciência e a Tecnologia (FCT) grants PTDC/SAU-MIC/122082/2010 and PTDC/BIA-BCM/105610/2008 to GRM, and SFRH/BPD/72619/2010 to PAGCS.</description><subject>Animals</subject><subject>Aquatic insects</subject><subject>Binding proteins</subject><subject>Biology and Life Sciences</subject><subject>Causes of</subject><subject>Chemical properties</subject><subject>Drosophila</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Host-Parasite Interactions</subject><subject>Immunoprecipitation</subject><subject>Infectious diseases</subject><subject>Insects</subject><subject>Laboratory animals</subject><subject>Liver</subject><subject>Malaria</subject><subject>Medicine and Health Sciences</subject><subject>Mosquitoes</subject><subject>mRNA</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>Physiological aspects</subject><subject>Plasmodium (Protozoa)</subject><subject>Plasmodium berghei</subject><subject>Plasmodium berghei - metabolism</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-binding protein</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Salivary gland</subject><subject>Salivary Glands - metabolism</subject><subject>Sporozoites</subject><subject>Sporozoites - metabolism</subject><subject>Translation</subject><subject>Trends</subject><subject>Vector-borne diseases</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEolB4AwRBSAgudrEdx45vkKqKw0oVRT1wa02S8a6rbBxsp6I8Bk-M091WXdQLlAufvvnt-TOTZS8omdNC0g8XbvQ9dPPB9TgnlEvFyYPsCVUFmwlGiod35nvZ0xAuCCmLSojH2R4TFamElE-yP2ce-tBBtC6J5Y3ro3dd7kx-vjjl-eBdRNtP67jCfOVCnA3gIdiIue0jegNNmoV8ja2FiG1eX12jJ98O8tr2re2XtyrfR8PyaewgrF1rx3Veo1-u0OZhcN79dkk2PMseGegCPt-O-9n5509nh19nR8dfFocHR7NGVjzOJFSCcQIppYoXbVUTUauSVUpRoxgFwpoWFRWVqQsk3KBoKSlURVFBTVVd7GevNrpD54Le2hk0lYIQVtGSJmKxIVoHF3rwdg3-Sjuw-nrD-aUGH23Toa4lAG0LMGUDvGRMGWOUMlJSCdiIIml93N421smrBpPR0O2I7p70dqWX7lJzyUQpSBJ4txXw7ueIIeq1DQ12HfToxs27U96CqYS--Qe9P7sttYSUgO2NS_c2k6g-4JwWRUmFTNT8Hip9La5tKhc0Nu3vBLzfCZhKCn_FJYwh6MXpyf-zxz922bd32BVCF1fBdeNUuWEX5Buw8S4Ej-bWZEr01Do3buipdfS2dVLYy7s_6DboplcS8HoD-AZg0B4vbYiQLCW8pLq8dv4v7sAfaA</recordid><startdate>20160125</startdate><enddate>20160125</enddate><creator>Silva, Patrícia A. 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G. C.</au><au>dos Santos Guerreiro, Ana Maria</au><au>Santos, Jorge</au><au>Braks, Joanna A. M.</au><au>Janse, Chris J.</au><au>Mair, Gunnar</au><au>Snounou, Georges</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Translational control of UIS4 protein of the host-parasite interface is mediated by the RNA binding protein Puf2 in Plasmodium berghei sporozoites</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-01-25</date><risdate>2016</risdate><volume>11</volume><issue>1</issue><spage>e0147940</spage><epage>e0147940</epage><pages>e0147940-e0147940</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Copyright: © 2016 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UIS4 is a key protein component of the host-parasite interface in the liver stage of the rodent malaria parasite Plasmodium berghei and required for parasite survival after invasion. In the infectious sporozoite, UIS4 protein has variably been shown to be translated but also been reported to be translationally repressed. Here we show that uis4 mRNA translation is regulated by the P. berghei RNA binding protein Pumilio-2 (PbPuf2 or Puf2 from here on forward) in infectious salivary gland sporozoites in the mosquito vector. Using RNA immunoprecipitation we show that uis4 mRNA is bound by Puf2 in salivary gland sporozoites. In the absence of Puf2, uis4 mRNA translation is de-regulated and UIS4 protein expression upregulated in salivary gland sporozoites. Here, using RNA immunoprecipitation, we reveal the first Puf2-regulated mRNA in this parasite.
This work was supported by Fundação para a Ciência e a Tecnologia (FCT) grants PTDC/SAU-MIC/122082/2010 and PTDC/BIA-BCM/105610/2008 to GRM, and SFRH/BPD/72619/2010 to PAGCS.</abstract><cop>United States</cop><pub>PLoS</pub><pmid>26808677</pmid><doi>10.1371/journal.pone.0147940</doi><tpages>e0147940</tpages><orcidid>https://orcid.org/0000-0002-4940-5207</orcidid><orcidid>https://orcid.org/0000-0002-5187-3691</orcidid><orcidid>https://orcid.org/0000-0002-2173-6514</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Aquatic insects Binding proteins Biology and Life Sciences Causes of Chemical properties Drosophila Gene expression Gene Expression Regulation Genes Genetic aspects Genetic regulation Host-Parasite Interactions Immunoprecipitation Infectious diseases Insects Laboratory animals Liver Malaria Medicine and Health Sciences Mosquitoes mRNA Parasites Parasitology Physiological aspects Plasmodium (Protozoa) Plasmodium berghei Plasmodium berghei - metabolism Protein expression Proteins Protozoan Proteins - genetics Protozoan Proteins - metabolism Ribonucleic acid RNA RNA-binding protein RNA-Binding Proteins - metabolism Salivary gland Salivary Glands - metabolism Sporozoites Sporozoites - metabolism Translation Trends Vector-borne diseases |
| title | Translational control of UIS4 protein of the host-parasite interface is mediated by the RNA binding protein Puf2 in Plasmodium berghei sporozoites |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T21%3A47%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Translational%20control%20of%20UIS4%20protein%20of%20the%20host-parasite%20interface%20is%20mediated%20by%20the%20RNA%20binding%20protein%20Puf2%20in%20Plasmodium%20berghei%20sporozoites&rft.jtitle=PloS%20one&rft.au=Silva,%20Patr%C3%ADcia%20A.%20G.%20C.&rft.date=2016-01-25&rft.volume=11&rft.issue=1&rft.spage=e0147940&rft.epage=e0147940&rft.pages=e0147940-e0147940&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0147940&rft_dat=%3Cgale_plos_%3EA441335167%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1760028151&rft_id=info:pmid/26808677&rft_galeid=A441335167&rft_doaj_id=oai_doaj_org_article_b7aa1d3af5ca45229fff99f7717aec63&rfr_iscdi=true |