Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in...
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| Veröffentlicht in: | PloS one 2015-12, Vol.10 (12), p.e0141479-e0141479 |
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| creator | Sridharan, BanuPriya Lin, Staphany M Hwu, Alexander T Laflin, Amy D Detamore, Michael S |
| description | There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton's jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance. |
| doi_str_mv | 10.1371/journal.pone.0141479 |
| format | Article |
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Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton's jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0141479</identifier><identifier>PMID: 26719986</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aggregates ; Animals ; Bioengineering ; Biomedical materials ; Biosynthesis ; Bone marrow ; Care and treatment ; Cartilage ; Cell culture ; Cell Culture Techniques ; Cell death ; Cell density ; Cell Differentiation ; Cell size ; Cell Survival ; Cells, Cultured ; Chondrogenesis ; Clinical trials ; Collagen ; Collagen - metabolism ; Consent ; Gels (Pharmacy) ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Health aspects ; Humans ; Hydrogels ; Hypotheses ; Medical research ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - physiology ; Mesenchyme ; Methods ; Penicillin ; Petroleum engineering ; Rats ; Regenerative medicine ; Review boards ; Stem cell transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - physiology ; Tissue engineering ; Umbilical cord ; Umbilical Cord - cytology</subject><ispartof>PloS one, 2015-12, Vol.10 (12), p.e0141479-e0141479</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Sridharan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Sridharan et al 2015 Sridharan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-432114125f79c242c99beb631abf570550f8ee21aeef76d9706015f63bfb54fe3</citedby><cites>FETCH-LOGICAL-c692t-432114125f79c242c99beb631abf570550f8ee21aeef76d9706015f63bfb54fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697858/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697858/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26719986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Engler, Adam J.</contributor><creatorcontrib>Sridharan, BanuPriya</creatorcontrib><creatorcontrib>Lin, Staphany M</creatorcontrib><creatorcontrib>Hwu, Alexander T</creatorcontrib><creatorcontrib>Laflin, Amy D</creatorcontrib><creatorcontrib>Detamore, Michael S</creatorcontrib><title>Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton's jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.</description><subject>Aggregates</subject><subject>Animals</subject><subject>Bioengineering</subject><subject>Biomedical materials</subject><subject>Biosynthesis</subject><subject>Bone marrow</subject><subject>Care and treatment</subject><subject>Cartilage</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell death</subject><subject>Cell density</subject><subject>Cell Differentiation</subject><subject>Cell size</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Chondrogenesis</subject><subject>Clinical trials</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Consent</subject><subject>Gels (Pharmacy)</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hypotheses</subject><subject>Medical research</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sridharan, BanuPriya</au><au>Lin, Staphany M</au><au>Hwu, Alexander T</au><au>Laflin, Amy D</au><au>Detamore, Michael S</au><au>Engler, Adam J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-12-31</date><risdate>2015</risdate><volume>10</volume><issue>12</issue><spage>e0141479</spage><epage>e0141479</epage><pages>e0141479-e0141479</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton's jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26719986</pmid><doi>10.1371/journal.pone.0141479</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aggregates Animals Bioengineering Biomedical materials Biosynthesis Bone marrow Care and treatment Cartilage Cell culture Cell Culture Techniques Cell death Cell density Cell Differentiation Cell size Cell Survival Cells, Cultured Chondrogenesis Clinical trials Collagen Collagen - metabolism Consent Gels (Pharmacy) Gene expression Gene Expression Profiling Gene Expression Regulation Health aspects Humans Hydrogels Hypotheses Medical research Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - physiology Mesenchyme Methods Penicillin Petroleum engineering Rats Regenerative medicine Review boards Stem cell transplantation Stem cells Stem Cells - cytology Stem Cells - physiology Tissue engineering Umbilical cord Umbilical Cord - cytology |
| title | Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels |
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