Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques

In the present study, a 2,4-dichlorophenoxyacetic acid (2,4-D) degrading bacterial strain CY-1 was isolated from the forest soil. Based on physiological, biochemical and 16S rRNA gene sequence analysis it was identified as Cupriavidus sp. CY-1. Further 2,4-D degradation experiments at different conc...

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Veröffentlicht in:	PloS one 2015-12, Vol.10 (12), p.e0145057-e0145057
Hauptverfasser:	Chang, Young-Cheol, Reddy, M Venkateswar, Umemoto, Honoka, Sato, Yuki, Kang, Mi-Hye, Yajima, Yuka, Kikuchi, Shintaro
Format:	Artikel
Sprache:	eng
Schlagworte:	2,4-D 2,4-D (Herbicide) 2,4-Dichlorophenoxyacetic Acid - metabolism Acids Augmentation Bacteria Biodegradation Biodegradation, Environmental Biodiversity Bioengineering Bioremediation Chloride ions Chromatography Chromatography, High Pressure Liquid Culture Cupriavidus - genetics Cupriavidus - isolation & purification Cupriavidus - metabolism Dechlorination Degradation Denaturing Gradient Gel Electrophoresis Dichlorophenoxyacetic acid DNA, Bacterial - genetics Ecosystem Engineering Environmental aspects Environmental degradation Environmental engineering Forest soils Gas Chromatography-Mass Spectrometry Gel electrophoresis Herbicides Herbicides - metabolism High performance liquid chromatography High pressure Liquid chromatography Metabolites Microorganisms Most probable number Nitrates - chemistry Physiological aspects Polymorphism Polymorphism, Restriction Fragment Length Potassium Compounds - chemistry Restriction fragment length polymorphism RNA, Ribosomal, 16S - genetics rRNA 16S Sediment pollution Sodium chloride Sodium Chloride - chemistry Soil - chemistry Soil analysis Soil contamination Soil Microbiology Soil microorganisms Soil Pollutants - metabolism Soil pollution Studies Survivability
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