Codon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System

By introducing synonymous mutations into the coding sequences of GP64sp and FibHsp signal peptides, the influences of mRNA secondary structure and codon usage of signal sequences on protein expression and secretion were investigated using baculovirus/insect cell expression system. The results showed...

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Veröffentlicht in:	PloS one 2015-12, Vol.10 (12), p.e0145887-e0145887
Hauptverfasser:	Wang, Yalan, Mao, Yuanhui, Xu, Xiaodong, Tao, Shiheng, Chen, Hongying
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Analysis Animals Baculoviridae - genetics Baculovirus Baculoviruses Base Pairing Base Sequence Biology Codon - genetics Codon bias Codons Degradation E coli Escherichia coli Gene expression Gene sequencing Genomes Infections Insect cells Insects Laboratories Life sciences Luciferase Luciferases - metabolism Molecular Sequence Data mRNA stability Mutation Peptides Protein Biosynthesis Protein expression Protein folding Protein Processing, Post-Translational Protein Sorting Signals - genetics Protein structure Proteins Proteins - genetics Proteins - metabolism Proteolysis RNA Stability - genetics Secondary structure Secretion Signal peptides Spodoptera - cytology Spodoptera - genetics Spodoptera - virology Streptomyces Structural stability
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