Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus
Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% of Western-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seiz...
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| Veröffentlicht in: | PloS one 2015-12, Vol.10 (12), p.e0145247-e0145247 |
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| creator | Jongbloets, Bart C van Gassen, Koen L I Kan, Anne A Olde Engberink, Anneke H O de Wit, Marina Wolterink-Donselaar, Inge G Groot Koerkamp, Marian J A van Nieuwenhuizen, Onno Holstege, Frank C P de Graan, Pierre N E |
| description | Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% of Western-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsion-susceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice. We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS. We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice. We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short- and long-term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis. |
| doi_str_mv | 10.1371/journal.pone.0145247 |
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Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsion-susceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice. We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS. We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice. We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short- and long-term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0145247</identifier><identifier>PMID: 26684451</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>2',3'-Cyclic Nucleotide 3'-Phosphodiesterase - genetics ; 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase - metabolism ; Animals ; CA1 Region, Hippocampal - metabolism ; CA1 Region, Hippocampal - pathology ; CA3 Region, Hippocampal - metabolism ; CA3 Region, Hippocampal - pathology ; Children ; Convulsions & seizures ; Development and progression ; Epilepsy ; Epilepsy, Temporal Lobe - metabolism ; Epilepsy, Temporal Lobe - pathology ; Febrile seizures ; Female ; Fever ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Gene Ontology ; Genetic aspects ; Genomes ; Glutamine ; Heat-Shock Response ; Hippocampus ; Hippocampus (Brain) ; Histology ; Hyperthermia ; Inflammation ; Long-term effects ; Male ; Medical research ; Mice ; Mice, Inbred C57BL ; Myelination ; Neurofilament Proteins - genetics ; Neurofilament Proteins - metabolism ; Neurosciences ; Physiological aspects ; Rodents ; Seizing ; Seizures ; Seizures, Febrile - metabolism ; Seizures, Febrile - pathology ; Temporal lobe ; Transcriptome ; Up-Regulation</subject><ispartof>PloS one, 2015-12, Vol.10 (12), p.e0145247-e0145247</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Jongbloets et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Jongbloets et al 2015 Jongbloets et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c585t-b567a396560440f5776fade5efa1511aef9491a4f778fa9a63b98e4ae968087a3</citedby><cites>FETCH-LOGICAL-c585t-b567a396560440f5776fade5efa1511aef9491a4f778fa9a63b98e4ae968087a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684321/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684321/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26684451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jongbloets, Bart C</creatorcontrib><creatorcontrib>van Gassen, Koen L I</creatorcontrib><creatorcontrib>Kan, Anne A</creatorcontrib><creatorcontrib>Olde Engberink, Anneke H O</creatorcontrib><creatorcontrib>de Wit, Marina</creatorcontrib><creatorcontrib>Wolterink-Donselaar, Inge G</creatorcontrib><creatorcontrib>Groot Koerkamp, Marian J A</creatorcontrib><creatorcontrib>van Nieuwenhuizen, Onno</creatorcontrib><creatorcontrib>Holstege, Frank C P</creatorcontrib><creatorcontrib>de Graan, Pierre N E</creatorcontrib><title>Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% of Western-European children). 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We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. 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genetics</subject><subject>Neurofilament Proteins - metabolism</subject><subject>Neurosciences</subject><subject>Physiological aspects</subject><subject>Rodents</subject><subject>Seizing</subject><subject>Seizures</subject><subject>Seizures, Febrile - metabolism</subject><subject>Seizures, Febrile - pathology</subject><subject>Temporal lobe</subject><subject>Transcriptome</subject><subject>Up-Regulation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptkl9v0zAUxSMEYmPwDRBEQkK8tNjxv_gFaZo2NmkIxODZuk2vU1dunNkJAsSHx22zqUM8xbF_9_je41MULymZU6bo-3UYYwd-3ocO54RyUXH1qDimmlUzWRH2-GB9VDxLaU2IYLWUT4ujSsqac0GPiz_nP_uIKbnQlV9isM67ri3BDhi3_z50LS7LDGF0G-wG8OUFLqLzWN6g-z3m2tJ15SfX5I2xbTENqbwZ4tgMY8zwV9yEJe5EMzassLx0fR8a2PRjel48seATvpi-J8X3i_NvZ5ez688fr85Or2eNqMUwWwipgGkpJOGcWKGUtLBEgRaooBTQaq4pcKtUbUGDZAtdIwfUsiZ1Lj0pXu91ex-SmYxLhipBqNZCy0xc7YllgLXp86wQf5kAzuw2QmwNxME1Hg3JmoJD1QBUvFlIkBXUhFVMV1Sous5aH6bbxsUGl012LTvxQPThSedWpg0_DM-PwiqaBd5NAjHcjtlRs3GpQe-hwzDu-qZUZzNURt_8g_5_uolqIQ_gOhvyvc1W1JxypuosRLd9vz2gVgh-WKXgxyFnIz0E-R5sYkgpor2fjRKzzeZdE2abTTNlM5e9OvTlvugujOwvDKfh_g</recordid><startdate>20151218</startdate><enddate>20151218</enddate><creator>Jongbloets, Bart C</creator><creator>van Gassen, Koen L I</creator><creator>Kan, Anne A</creator><creator>Olde Engberink, Anneke H O</creator><creator>de Wit, Marina</creator><creator>Wolterink-Donselaar, Inge G</creator><creator>Groot Koerkamp, Marian J A</creator><creator>van Nieuwenhuizen, Onno</creator><creator>Holstege, Frank C P</creator><creator>de Graan, Pierre N E</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20151218</creationdate><title>Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus</title><author>Jongbloets, Bart C ; van Gassen, Koen L I ; Kan, Anne A ; Olde Engberink, Anneke H O ; de Wit, Marina ; Wolterink-Donselaar, Inge G ; Groot Koerkamp, Marian J A ; van Nieuwenhuizen, Onno ; Holstege, Frank C P ; de Graan, Pierre N E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-b567a396560440f5776fade5efa1511aef9491a4f778fa9a63b98e4ae968087a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>2',3'-Cyclic Nucleotide 3'-Phosphodiesterase - 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Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsion-susceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice. We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS. We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice. We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short- and long-term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26684451</pmid><doi>10.1371/journal.pone.0145247</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-12, Vol.10 (12), p.e0145247-e0145247 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1750199596 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase - genetics 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase - metabolism Animals CA1 Region, Hippocampal - metabolism CA1 Region, Hippocampal - pathology CA3 Region, Hippocampal - metabolism CA3 Region, Hippocampal - pathology Children Convulsions & seizures Development and progression Epilepsy Epilepsy, Temporal Lobe - metabolism Epilepsy, Temporal Lobe - pathology Febrile seizures Female Fever Gene expression Gene Expression Profiling Gene Expression Regulation Gene Ontology Genetic aspects Genomes Glutamine Heat-Shock Response Hippocampus Hippocampus (Brain) Histology Hyperthermia Inflammation Long-term effects Male Medical research Mice Mice, Inbred C57BL Myelination Neurofilament Proteins - genetics Neurofilament Proteins - metabolism Neurosciences Physiological aspects Rodents Seizing Seizures Seizures, Febrile - metabolism Seizures, Febrile - pathology Temporal lobe Transcriptome Up-Regulation |
| title | Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T09%3A10%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20Profiling%20after%20Prolonged%20Experimental%20Febrile%20Seizures%20in%20Mice%20Suggests%20Structural%20Remodeling%20in%20the%20Hippocampus&rft.jtitle=PloS%20one&rft.au=Jongbloets,%20Bart%20C&rft.date=2015-12-18&rft.volume=10&rft.issue=12&rft.spage=e0145247&rft.epage=e0145247&rft.pages=e0145247-e0145247&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0145247&rft_dat=%3Cgale_plos_%3EA437847318%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1750199596&rft_id=info:pmid/26684451&rft_galeid=A437847318&rft_doaj_id=oai_doaj_org_article_080854a2caa24cb6a62a803239215788&rfr_iscdi=true |