KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we repo...
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| Veröffentlicht in: | PloS one 2015-12, Vol.10 (12), p.e0145287 |
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| creator | Sarkisyan, Karen S Zlobovskaya, Olga A Gorbachev, Dmitry A Bozhanova, Nina G Sharonov, George V Staroverov, Dmitriy B Egorov, Evgeny S Ryabova, Anastasia V Solntsev, Kyril M Mishin, Alexander S Lukyanov, Konstantin A |
| description | Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity. |
| doi_str_mv | 10.1371/journal.pone.0145287 |
| format | Article |
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Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. 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This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Sarkisyan et al 2015 Sarkisyan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-5893fd1fa3773109040ae244811841f085d6639c300358e929efac81bdcdba5e3</citedby><cites>FETCH-LOGICAL-c758t-5893fd1fa3773109040ae244811841f085d6639c300358e929efac81bdcdba5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683004/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683004/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26679300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarkisyan, Karen S</creatorcontrib><creatorcontrib>Zlobovskaya, Olga A</creatorcontrib><creatorcontrib>Gorbachev, Dmitry A</creatorcontrib><creatorcontrib>Bozhanova, Nina G</creatorcontrib><creatorcontrib>Sharonov, George V</creatorcontrib><creatorcontrib>Staroverov, Dmitriy B</creatorcontrib><creatorcontrib>Egorov, Evgeny S</creatorcontrib><creatorcontrib>Ryabova, Anastasia V</creatorcontrib><creatorcontrib>Solntsev, Kyril M</creatorcontrib><creatorcontrib>Mishin, Alexander S</creatorcontrib><creatorcontrib>Lukyanov, Konstantin A</creatorcontrib><title>KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. 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Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26679300</pmid><doi>10.1371/journal.pone.0145287</doi><tpages>e0145287</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ablation Bacteria Biochemistry Biology Cell division Cells (Biology) Cellular proteins Chemistry Chromophores Coding Deactivation Endangered & extinct species Fluorescence Genetic code Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics HEK293 Cells Humans Inactivation Light Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - radiation effects Luminescent Proteins - toxicity Mammalian cells Mutagenesis Mutation Orthogonality Oxygen Photosensitizing Agents - chemistry Photosensitizing Agents - radiation effects Photosensitizing Agents - toxicity Proteins Reactive oxygen species Red Fluorescent Protein Toxicity Tryptophan Ultraviolet Rays |
| title | KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light |
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