West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling...
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| Veröffentlicht in: | PloS one 2015-11, Vol.10 (11), p.e0143803-e0143803 |
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| creator | Hadjichristodoulou, Christos Pournaras, Spyros Mavrouli, Maria Marka, Andriani Tserkezou, Persefoni Baka, Agoritsa Billinis, Charalambos Katsioulis, Antonios Psaroulaki, Anna Papa, Anna Papadopoulos, Nikos Mamuris, Zissis Tsakris, Athanasios Kremastinou, Jenny |
| description | Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7-2.6) and 1.5% (95% C.I.: 1.2-2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421-1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease. |
| doi_str_mv | 10.1371/journal.pone.0143803 |
| format | Article |
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A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7-2.6) and 1.5% (95% C.I.: 1.2-2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421-1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0143803</identifier><identifier>PMID: 26605539</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies ; Antibodies, Viral - blood ; Antibodies, Viral - immunology ; Bird migration ; Blood & organ donations ; Care and treatment ; Child ; Child, Preschool ; Cross-Sectional Studies ; Disease control ; Disease prevention ; Enzyme-linked immunosorbent assay ; Epidemics ; Epidemiology ; Geography ; Geriatrics ; Greece - epidemiology ; Health care ; Health risks ; Humans ; Hygiene ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Immunoglobulin M ; Immunoglobulins ; Infant ; Infant, Newborn ; Infections ; Invasiveness ; Medical laboratories ; Middle Aged ; Mosquitoes ; Neutralization ; Older people ; Outbreaks ; Parasitology ; Population ; Population Surveillance ; Risk factors ; Sample size ; Sampling methods ; Seroepidemiologic Studies ; Serology ; Viruses ; West Nile fever ; West Nile Fever - diagnosis ; West Nile Fever - epidemiology ; West Nile Fever - immunology ; West Nile Fever - virology ; West Nile virus ; West Nile virus - immunology ; Young Adult</subject><ispartof>PloS one, 2015-11, Vol.10 (11), p.e0143803-e0143803</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Hadjichristodoulou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Hadjichristodoulou et al 2015 Hadjichristodoulou et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-7559f6705fcd78f3760df56ec4bfecd483f229c85230ee5776ccbda34d92e4a03</citedby><cites>FETCH-LOGICAL-c692t-7559f6705fcd78f3760df56ec4bfecd483f229c85230ee5776ccbda34d92e4a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659653/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659653/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26605539$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Alexopoulou, Lena</contributor><creatorcontrib>Hadjichristodoulou, Christos</creatorcontrib><creatorcontrib>Pournaras, Spyros</creatorcontrib><creatorcontrib>Mavrouli, Maria</creatorcontrib><creatorcontrib>Marka, Andriani</creatorcontrib><creatorcontrib>Tserkezou, Persefoni</creatorcontrib><creatorcontrib>Baka, Agoritsa</creatorcontrib><creatorcontrib>Billinis, Charalambos</creatorcontrib><creatorcontrib>Katsioulis, Antonios</creatorcontrib><creatorcontrib>Psaroulaki, Anna</creatorcontrib><creatorcontrib>Papa, Anna</creatorcontrib><creatorcontrib>Papadopoulos, Nikos</creatorcontrib><creatorcontrib>Mamuris, Zissis</creatorcontrib><creatorcontrib>Tsakris, Athanasios</creatorcontrib><creatorcontrib>Kremastinou, Jenny</creatorcontrib><creatorcontrib>MALWEST Project</creatorcontrib><title>West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7-2.6) and 1.5% (95% C.I.: 1.2-2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421-1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - immunology</subject><subject>Bird migration</subject><subject>Blood & organ donations</subject><subject>Care and treatment</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross-Sectional Studies</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Geography</subject><subject>Geriatrics</subject><subject>Greece - epidemiology</subject><subject>Health care</subject><subject>Health risks</subject><subject>Humans</subject><subject>Hygiene</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulins</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infections</subject><subject>Invasiveness</subject><subject>Medical laboratories</subject><subject>Middle Aged</subject><subject>Mosquitoes</subject><subject>Neutralization</subject><subject>Older people</subject><subject>Outbreaks</subject><subject>Parasitology</subject><subject>Population</subject><subject>Population Surveillance</subject><subject>Risk factors</subject><subject>Sample size</subject><subject>Sampling methods</subject><subject>Seroepidemiologic Studies</subject><subject>Serology</subject><subject>Viruses</subject><subject>West Nile fever</subject><subject>West Nile Fever - diagnosis</subject><subject>West Nile Fever - epidemiology</subject><subject>West Nile Fever - immunology</subject><subject>West Nile Fever - virology</subject><subject>West Nile virus</subject><subject>West Nile virus - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hadjichristodoulou, Christos</au><au>Pournaras, Spyros</au><au>Mavrouli, Maria</au><au>Marka, Andriani</au><au>Tserkezou, Persefoni</au><au>Baka, Agoritsa</au><au>Billinis, Charalambos</au><au>Katsioulis, Antonios</au><au>Psaroulaki, Anna</au><au>Papa, Anna</au><au>Papadopoulos, Nikos</au><au>Mamuris, Zissis</au><au>Tsakris, Athanasios</au><au>Kremastinou, Jenny</au><au>Alexopoulou, Lena</au><aucorp>MALWEST Project</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-11-25</date><risdate>2015</risdate><volume>10</volume><issue>11</issue><spage>e0143803</spage><epage>e0143803</epage><pages>e0143803-e0143803</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme-Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7-2.6) and 1.5% (95% C.I.: 1.2-2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421-1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26605539</pmid><doi>10.1371/journal.pone.0143803</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-11, Vol.10 (11), p.e0143803-e0143803 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1736407771 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Aged Aged, 80 and over Antibodies Antibodies, Viral - blood Antibodies, Viral - immunology Bird migration Blood & organ donations Care and treatment Child Child, Preschool Cross-Sectional Studies Disease control Disease prevention Enzyme-linked immunosorbent assay Epidemics Epidemiology Geography Geriatrics Greece - epidemiology Health care Health risks Humans Hygiene Immunoglobulin G Immunoglobulin G - blood Immunoglobulin G - immunology Immunoglobulin M Immunoglobulins Infant Infant, Newborn Infections Invasiveness Medical laboratories Middle Aged Mosquitoes Neutralization Older people Outbreaks Parasitology Population Population Surveillance Risk factors Sample size Sampling methods Seroepidemiologic Studies Serology Viruses West Nile fever West Nile Fever - diagnosis West Nile Fever - epidemiology West Nile Fever - immunology West Nile Fever - virology West Nile virus West Nile virus - immunology Young Adult |
| title | West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-03T13%3A31%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=West%20Nile%20Virus%20Seroprevalence%20in%20the%20Greek%20Population%20in%202013:%20A%20Nationwide%20Cross-Sectional%20Survey&rft.jtitle=PloS%20one&rft.au=Hadjichristodoulou,%20Christos&rft.aucorp=MALWEST%20Project&rft.date=2015-11-25&rft.volume=10&rft.issue=11&rft.spage=e0143803&rft.epage=e0143803&rft.pages=e0143803-e0143803&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0143803&rft_dat=%3Cgale_plos_%3EA435601859%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1736407771&rft_id=info:pmid/26605539&rft_galeid=A435601859&rft_doaj_id=oai_doaj_org_article_5b4ffd802f814f86bb283855cf18fb14&rfr_iscdi=true |