Acute Heat Stress and Reduced Nutrient Intake Alter Intestinal Proteomic Profile and Gene Expression in Pigs
Heat stress and reduced feed intake negatively affect intestinal integrity and barrier function. Our objective was to compare ileum protein profiles of pigs subjected to 12 hours of HS, thermal neutral ad libitum feed intake, or pair-fed to heat stress feed intake under thermal neutral conditions (p...
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| Veröffentlicht in: | PloS one 2015-11, Vol.10 (11), p.e0143099-e0143099 |
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| creator | Pearce, Sarah C Lonergan, Steven M Huff-Lonergan, Elisabeth Baumgard, Lance H Gabler, Nicholas K |
| description | Heat stress and reduced feed intake negatively affect intestinal integrity and barrier function. Our objective was to compare ileum protein profiles of pigs subjected to 12 hours of HS, thermal neutral ad libitum feed intake, or pair-fed to heat stress feed intake under thermal neutral conditions (pair-fed thermal neutral). 2D-Differential In Gel Electrophoresis and gene expression were performed. Relative abundance of 281 and 138 spots differed due to heat stress, compared to thermal neutral and pair-fed thermal neutral pigs, respectively. However, only 20 proteins were different due to feed intake (thermal neutral versus pair-fed thermal neutral). Heat stress increased mRNA expression of heat shock proteins and protein abundance of heat shock proteins 27, 70, 90-α and β were also increased. Heat stress reduced ileum abundance of several metabolic enzymes, many of which are involved in the glycolytic or TCA pathways, indicating a change in metabolic priorities. Stress response enzymes peroxiredoxin-1 and peptidyl-prolyl cis-trans isomerase A were decreased in pair-fed thermal neutral and thermal neutral pigs compared to heat stress. Heat stress increased mRNA abundance markers of ileum hypoxia. Altogether, these data show that heat stress directly alters intestinal protein and mRNA profiles largely independent of reduced feed intake. These changes may be related to the reduced intestinal integrity associated with heat stress. |
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Our objective was to compare ileum protein profiles of pigs subjected to 12 hours of HS, thermal neutral ad libitum feed intake, or pair-fed to heat stress feed intake under thermal neutral conditions (pair-fed thermal neutral). 2D-Differential In Gel Electrophoresis and gene expression were performed. Relative abundance of 281 and 138 spots differed due to heat stress, compared to thermal neutral and pair-fed thermal neutral pigs, respectively. However, only 20 proteins were different due to feed intake (thermal neutral versus pair-fed thermal neutral). Heat stress increased mRNA expression of heat shock proteins and protein abundance of heat shock proteins 27, 70, 90-α and β were also increased. Heat stress reduced ileum abundance of several metabolic enzymes, many of which are involved in the glycolytic or TCA pathways, indicating a change in metabolic priorities. Stress response enzymes peroxiredoxin-1 and peptidyl-prolyl cis-trans isomerase A were decreased in pair-fed thermal neutral and thermal neutral pigs compared to heat stress. Heat stress increased mRNA abundance markers of ileum hypoxia. Altogether, these data show that heat stress directly alters intestinal protein and mRNA profiles largely independent of reduced feed intake. These changes may be related to the reduced intestinal integrity associated with heat stress.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0143099</identifier><identifier>PMID: 26575181</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abundance ; Animal sciences ; Animals ; Cellular stress response ; Energy Intake ; Enzymes ; Feeds ; Female ; Gel electrophoresis ; Gene Expression ; Glycolysis ; Heat ; Heat shock proteins ; Heat stress ; Heat tolerance ; Heat-Shock Response ; Heatstroke ; Hogs ; HSP70 Heat-Shock Proteins - metabolism ; Humidity ; Hypoxia ; Ileum ; Ileum - metabolism ; Integrity ; Intestine ; Metabolism ; Peptidylprolyl isomerase ; Peroxiredoxin ; Proteins ; Proteome - metabolism ; Relative abundance ; Respiration ; Rodents ; Small intestine ; Suidae ; Sus scrofa ; Viruses ; Zoology</subject><ispartof>PloS one, 2015-11, Vol.10 (11), p.e0143099-e0143099</ispartof><rights>2015 Pearce et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Pearce et al 2015 Pearce et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-348551fa336b435b9206efa5432c5ebcee4f42e7b9256b5c828a645098420f33</citedby><cites>FETCH-LOGICAL-c526t-348551fa336b435b9206efa5432c5ebcee4f42e7b9256b5c828a645098420f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648527/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648527/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26575181$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pearce, Sarah C</creatorcontrib><creatorcontrib>Lonergan, Steven M</creatorcontrib><creatorcontrib>Huff-Lonergan, Elisabeth</creatorcontrib><creatorcontrib>Baumgard, Lance H</creatorcontrib><creatorcontrib>Gabler, Nicholas K</creatorcontrib><title>Acute Heat Stress and Reduced Nutrient Intake Alter Intestinal Proteomic Profile and Gene Expression in Pigs</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Heat stress and reduced feed intake negatively affect intestinal integrity and barrier function. Our objective was to compare ileum protein profiles of pigs subjected to 12 hours of HS, thermal neutral ad libitum feed intake, or pair-fed to heat stress feed intake under thermal neutral conditions (pair-fed thermal neutral). 2D-Differential In Gel Electrophoresis and gene expression were performed. Relative abundance of 281 and 138 spots differed due to heat stress, compared to thermal neutral and pair-fed thermal neutral pigs, respectively. However, only 20 proteins were different due to feed intake (thermal neutral versus pair-fed thermal neutral). Heat stress increased mRNA expression of heat shock proteins and protein abundance of heat shock proteins 27, 70, 90-α and β were also increased. Heat stress reduced ileum abundance of several metabolic enzymes, many of which are involved in the glycolytic or TCA pathways, indicating a change in metabolic priorities. Stress response enzymes peroxiredoxin-1 and peptidyl-prolyl cis-trans isomerase A were decreased in pair-fed thermal neutral and thermal neutral pigs compared to heat stress. Heat stress increased mRNA abundance markers of ileum hypoxia. Altogether, these data show that heat stress directly alters intestinal protein and mRNA profiles largely independent of reduced feed intake. These changes may be related to the reduced intestinal integrity associated with heat stress.</description><subject>Abundance</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Cellular stress response</subject><subject>Energy Intake</subject><subject>Enzymes</subject><subject>Feeds</subject><subject>Female</subject><subject>Gel electrophoresis</subject><subject>Gene Expression</subject><subject>Glycolysis</subject><subject>Heat</subject><subject>Heat shock proteins</subject><subject>Heat stress</subject><subject>Heat tolerance</subject><subject>Heat-Shock Response</subject><subject>Heatstroke</subject><subject>Hogs</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Humidity</subject><subject>Hypoxia</subject><subject>Ileum</subject><subject>Ileum - metabolism</subject><subject>Integrity</subject><subject>Intestine</subject><subject>Metabolism</subject><subject>Peptidylprolyl isomerase</subject><subject>Peroxiredoxin</subject><subject>Proteins</subject><subject>Proteome - metabolism</subject><subject>Relative abundance</subject><subject>Respiration</subject><subject>Rodents</subject><subject>Small intestine</subject><subject>Suidae</subject><subject>Sus scrofa</subject><subject>Viruses</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAUjBCIlsI_QGCJC5dd_J34grSqSrtSBRX0bjnOy-LFGy-2g-Df4-ymVYs4-dmemffmaarqNcFLwmryYRvGOBi_3IcBlphwhpV6Up0SxehCUsyePqhPqhcpbTEWrJHyeXVCpagFachp5Vd2zICuwGT0LUdICZmhQ1-hGy106POYo4Mho_WQzQ9AK58hThdI2ZXu6CaGDGHn7FT1zsOBfgkDoIvf-0nPhQG5Ad24TXpZPeuNT_BqPs-q208Xt-dXi-svl-vz1fXCCirzgvFGCNIbxmTLmWgVxRJ6IzijVkBrAXjPKdTlQ8hW2IY2RnKBVcMp7hk7q94eZfc-JD3vKWlSM1YYBKuCWB8RXTBbvY9uZ-IfHYzTh4cQN9rE7KwHbXqFWymBMFtz01nVSNP0ignRYUmoLFof525ju4POlm1F4x-JPv4Z3He9Cb80l8UnrYvA-1kghp9jWazeuWTBezNAGA9zC4UlVZOzd_9A_--OH1E2hpQi9PfDEKyn7Nyx9JQdPWen0N48NHJPugsL-wvPLMHb</recordid><startdate>20151117</startdate><enddate>20151117</enddate><creator>Pearce, Sarah C</creator><creator>Lonergan, Steven M</creator><creator>Huff-Lonergan, Elisabeth</creator><creator>Baumgard, Lance H</creator><creator>Gabler, Nicholas K</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20151117</creationdate><title>Acute Heat Stress and Reduced Nutrient Intake Alter Intestinal Proteomic Profile and Gene Expression in Pigs</title><author>Pearce, Sarah C ; Lonergan, Steven M ; Huff-Lonergan, Elisabeth ; Baumgard, Lance H ; Gabler, Nicholas K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-348551fa336b435b9206efa5432c5ebcee4f42e7b9256b5c828a645098420f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abundance</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Cellular stress response</topic><topic>Energy Intake</topic><topic>Enzymes</topic><topic>Feeds</topic><topic>Female</topic><topic>Gel electrophoresis</topic><topic>Gene Expression</topic><topic>Glycolysis</topic><topic>Heat</topic><topic>Heat shock proteins</topic><topic>Heat stress</topic><topic>Heat tolerance</topic><topic>Heat-Shock Response</topic><topic>Heatstroke</topic><topic>Hogs</topic><topic>HSP70 Heat-Shock Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pearce, Sarah C</au><au>Lonergan, Steven M</au><au>Huff-Lonergan, Elisabeth</au><au>Baumgard, Lance H</au><au>Gabler, Nicholas K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute Heat Stress and Reduced Nutrient Intake Alter Intestinal Proteomic Profile and Gene Expression in Pigs</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-11-17</date><risdate>2015</risdate><volume>10</volume><issue>11</issue><spage>e0143099</spage><epage>e0143099</epage><pages>e0143099-e0143099</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Heat stress and reduced feed intake negatively affect intestinal integrity and barrier function. Our objective was to compare ileum protein profiles of pigs subjected to 12 hours of HS, thermal neutral ad libitum feed intake, or pair-fed to heat stress feed intake under thermal neutral conditions (pair-fed thermal neutral). 2D-Differential In Gel Electrophoresis and gene expression were performed. Relative abundance of 281 and 138 spots differed due to heat stress, compared to thermal neutral and pair-fed thermal neutral pigs, respectively. However, only 20 proteins were different due to feed intake (thermal neutral versus pair-fed thermal neutral). Heat stress increased mRNA expression of heat shock proteins and protein abundance of heat shock proteins 27, 70, 90-α and β were also increased. Heat stress reduced ileum abundance of several metabolic enzymes, many of which are involved in the glycolytic or TCA pathways, indicating a change in metabolic priorities. Stress response enzymes peroxiredoxin-1 and peptidyl-prolyl cis-trans isomerase A were decreased in pair-fed thermal neutral and thermal neutral pigs compared to heat stress. Heat stress increased mRNA abundance markers of ileum hypoxia. Altogether, these data show that heat stress directly alters intestinal protein and mRNA profiles largely independent of reduced feed intake. These changes may be related to the reduced intestinal integrity associated with heat stress.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26575181</pmid><doi>10.1371/journal.pone.0143099</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Abundance Animal sciences Animals Cellular stress response Energy Intake Enzymes Feeds Female Gel electrophoresis Gene Expression Glycolysis Heat Heat shock proteins Heat stress Heat tolerance Heat-Shock Response Heatstroke Hogs HSP70 Heat-Shock Proteins - metabolism Humidity Hypoxia Ileum Ileum - metabolism Integrity Intestine Metabolism Peptidylprolyl isomerase Peroxiredoxin Proteins Proteome - metabolism Relative abundance Respiration Rodents Small intestine Suidae Sus scrofa Viruses Zoology |
| title | Acute Heat Stress and Reduced Nutrient Intake Alter Intestinal Proteomic Profile and Gene Expression in Pigs |
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