Application of Pseudomonas fluorescens to Blackberry under Field Conditions Improves Fruit Quality by Modifying Flavonoid Metabolism
Application of a plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens N21.4, to roots of blackberries (Rubus sp.) is part of an optimised cultivation practice to improve yields and quality of fruit throughout the year in this important fruit crop. Blackberries are especially rich in...
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| creator | Garcia-Seco, Daniel Zhang, Yang Gutierrez-Mañero, Francisco J Martin, Cathie Ramos-Solano, Beatriz |
| description | Application of a plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens N21.4, to roots of blackberries (Rubus sp.) is part of an optimised cultivation practice to improve yields and quality of fruit throughout the year in this important fruit crop. Blackberries are especially rich in flavonoids and therefore offer potential benefits for human health in prevention or amelioration of chronic diseases. However, the phenylpropanoid pathway and its regulation during ripening have not been studied in detail, in this species. PGPR may trigger flavonoid biosynthesis as part of an induced systemic response (ISR) given the important role of this pathway in plant defence, to cause increased levels of flavonoids in the fruit. We have identified structural genes encoding enzymes of the phenylpropanoid and flavonoid biosynthetic pathways catalysing the conversion of phenylalanine to the final products including flavonols, anthocyanins and catechins from blackberry, and regulatory genes likely involved in controlling the activity of pathway branches. We have also measured the major flavonols, anthocyanins and catechins at three stages during ripening. Our results demonstrate the coordinated expression of flavonoid biosynthetic genes with the accumulation of anthocyanins, catechins, and flavonols in developing fruits of blackberry. Elicitation of blackberry plants by treatment of roots with P.fluorescens N21.4, caused increased expression of some flavonoid biosynthetic genes and an accompanying increase in the concentration of selected flavonoids in fruits. Our data demonstrate the physiological mechanisms involved in the improvement of fruit quality by PGPR under field conditions, and highlight some of the genetic targets of elicitation by beneficial bacteria. |
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Blackberries are especially rich in flavonoids and therefore offer potential benefits for human health in prevention or amelioration of chronic diseases. However, the phenylpropanoid pathway and its regulation during ripening have not been studied in detail, in this species. PGPR may trigger flavonoid biosynthesis as part of an induced systemic response (ISR) given the important role of this pathway in plant defence, to cause increased levels of flavonoids in the fruit. We have identified structural genes encoding enzymes of the phenylpropanoid and flavonoid biosynthetic pathways catalysing the conversion of phenylalanine to the final products including flavonols, anthocyanins and catechins from blackberry, and regulatory genes likely involved in controlling the activity of pathway branches. We have also measured the major flavonols, anthocyanins and catechins at three stages during ripening. Our results demonstrate the coordinated expression of flavonoid biosynthetic genes with the accumulation of anthocyanins, catechins, and flavonols in developing fruits of blackberry. Elicitation of blackberry plants by treatment of roots with P.fluorescens N21.4, caused increased expression of some flavonoid biosynthetic genes and an accompanying increase in the concentration of selected flavonoids in fruits. Our data demonstrate the physiological mechanisms involved in the improvement of fruit quality by PGPR under field conditions, and highlight some of the genetic targets of elicitation by beneficial bacteria.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0142639</identifier><identifier>PMID: 26559418</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agricultural production ; Anthocyanins ; Anthocyanins - chemistry ; Anthocyanins - metabolism ; Arabidopsis ; Bacteria ; Berries ; Biosynthesis ; Blackberries ; Botanical research ; Catechin ; Catechin - chemistry ; Catechin - metabolism ; Chlorophyll ; Chromatography, High Pressure Liquid ; Chronic illnesses ; Cultivation ; Data processing ; Flavonoids ; Flavonoids - chemistry ; Flavonoids - metabolism ; Flavonols ; Flavonols - chemistry ; Flavonols - metabolism ; Fruit - chemistry ; Fruit - metabolism ; Fruits ; Gene expression ; Genes ; Greenhouses ; Host-bacteria relationships ; Mass Spectrometry ; Metabolism ; Metabolites ; Observations ; Pathogenesis ; Phenylalanine ; Physiological aspects ; Plant growth ; Plant metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - growth & development ; Plant Roots - metabolism ; Plant Roots - microbiology ; Probiotics ; Properties ; Proteins ; Pseudomonas fluorescens ; Pseudomonas fluorescens - isolation & purification ; Real-Time Polymerase Chain Reaction ; Ripening ; Roots ; Rubus ; Rubus - chemistry ; Rubus - growth & development ; Rubus - metabolism ; Symbiosis - physiology</subject><ispartof>PloS one, 2015-11, Vol.10 (11), p.e0142639-e0142639</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Garcia-Seco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Garcia-Seco et al 2015 Garcia-Seco et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c653t-d735b9a5bfccc36e6465a8544d8574a2ab57a96b4791096e682ab90fbdc4a143</citedby><cites>FETCH-LOGICAL-c653t-d735b9a5bfccc36e6465a8544d8574a2ab57a96b4791096e682ab90fbdc4a143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641737/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641737/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26559418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia-Seco, Daniel</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Gutierrez-Mañero, Francisco J</creatorcontrib><creatorcontrib>Martin, Cathie</creatorcontrib><creatorcontrib>Ramos-Solano, Beatriz</creatorcontrib><title>Application of Pseudomonas fluorescens to Blackberry under Field Conditions Improves Fruit Quality by Modifying Flavonoid Metabolism</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Application of a plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens N21.4, to roots of blackberries (Rubus sp.) is part of an optimised cultivation practice to improve yields and quality of fruit throughout the year in this important fruit crop. Blackberries are especially rich in flavonoids and therefore offer potential benefits for human health in prevention or amelioration of chronic diseases. However, the phenylpropanoid pathway and its regulation during ripening have not been studied in detail, in this species. PGPR may trigger flavonoid biosynthesis as part of an induced systemic response (ISR) given the important role of this pathway in plant defence, to cause increased levels of flavonoids in the fruit. We have identified structural genes encoding enzymes of the phenylpropanoid and flavonoid biosynthetic pathways catalysing the conversion of phenylalanine to the final products including flavonols, anthocyanins and catechins from blackberry, and regulatory genes likely involved in controlling the activity of pathway branches. We have also measured the major flavonols, anthocyanins and catechins at three stages during ripening. Our results demonstrate the coordinated expression of flavonoid biosynthetic genes with the accumulation of anthocyanins, catechins, and flavonols in developing fruits of blackberry. Elicitation of blackberry plants by treatment of roots with P.fluorescens N21.4, caused increased expression of some flavonoid biosynthetic genes and an accompanying increase in the concentration of selected flavonoids in fruits. Our data demonstrate the physiological mechanisms involved in the improvement of fruit quality by PGPR under field conditions, and highlight some of the genetic targets of elicitation by beneficial bacteria.</description><subject>Agricultural production</subject><subject>Anthocyanins</subject><subject>Anthocyanins - chemistry</subject><subject>Anthocyanins - metabolism</subject><subject>Arabidopsis</subject><subject>Bacteria</subject><subject>Berries</subject><subject>Biosynthesis</subject><subject>Blackberries</subject><subject>Botanical research</subject><subject>Catechin</subject><subject>Catechin - chemistry</subject><subject>Catechin - metabolism</subject><subject>Chlorophyll</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chronic illnesses</subject><subject>Cultivation</subject><subject>Data processing</subject><subject>Flavonoids</subject><subject>Flavonoids - chemistry</subject><subject>Flavonoids - metabolism</subject><subject>Flavonols</subject><subject>Flavonols - 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Blackberries are especially rich in flavonoids and therefore offer potential benefits for human health in prevention or amelioration of chronic diseases. However, the phenylpropanoid pathway and its regulation during ripening have not been studied in detail, in this species. PGPR may trigger flavonoid biosynthesis as part of an induced systemic response (ISR) given the important role of this pathway in plant defence, to cause increased levels of flavonoids in the fruit. We have identified structural genes encoding enzymes of the phenylpropanoid and flavonoid biosynthetic pathways catalysing the conversion of phenylalanine to the final products including flavonols, anthocyanins and catechins from blackberry, and regulatory genes likely involved in controlling the activity of pathway branches. We have also measured the major flavonols, anthocyanins and catechins at three stages during ripening. Our results demonstrate the coordinated expression of flavonoid biosynthetic genes with the accumulation of anthocyanins, catechins, and flavonols in developing fruits of blackberry. Elicitation of blackberry plants by treatment of roots with P.fluorescens N21.4, caused increased expression of some flavonoid biosynthetic genes and an accompanying increase in the concentration of selected flavonoids in fruits. Our data demonstrate the physiological mechanisms involved in the improvement of fruit quality by PGPR under field conditions, and highlight some of the genetic targets of elicitation by beneficial bacteria.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26559418</pmid><doi>10.1371/journal.pone.0142639</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2015-11, Vol.10 (11), p.e0142639-e0142639 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agricultural production Anthocyanins Anthocyanins - chemistry Anthocyanins - metabolism Arabidopsis Bacteria Berries Biosynthesis Blackberries Botanical research Catechin Catechin - chemistry Catechin - metabolism Chlorophyll Chromatography, High Pressure Liquid Chronic illnesses Cultivation Data processing Flavonoids Flavonoids - chemistry Flavonoids - metabolism Flavonols Flavonols - chemistry Flavonols - metabolism Fruit - chemistry Fruit - metabolism Fruits Gene expression Genes Greenhouses Host-bacteria relationships Mass Spectrometry Metabolism Metabolites Observations Pathogenesis Phenylalanine Physiological aspects Plant growth Plant metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - growth & development Plant Roots - metabolism Plant Roots - microbiology Probiotics Properties Proteins Pseudomonas fluorescens Pseudomonas fluorescens - isolation & purification Real-Time Polymerase Chain Reaction Ripening Roots Rubus Rubus - chemistry Rubus - growth & development Rubus - metabolism Symbiosis - physiology |
| title | Application of Pseudomonas fluorescens to Blackberry under Field Conditions Improves Fruit Quality by Modifying Flavonoid Metabolism |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T11%3A02%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Application%20of%20Pseudomonas%20fluorescens%20to%20Blackberry%20under%20Field%20Conditions%20Improves%20Fruit%20Quality%20by%20Modifying%20Flavonoid%20Metabolism&rft.jtitle=PloS%20one&rft.au=Garcia-Seco,%20Daniel&rft.date=2015-11-11&rft.volume=10&rft.issue=11&rft.spage=e0142639&rft.epage=e0142639&rft.pages=e0142639-e0142639&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0142639&rft_dat=%3Cgale_plos_%3EA434300307%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1732577284&rft_id=info:pmid/26559418&rft_galeid=A434300307&rft_doaj_id=oai_doaj_org_article_2e3feffc82024b6eae677af864d6eab0&rfr_iscdi=true |