The Performance of the Four Anaerobic Blood Culture Bottles BacT/ALERT-FN, -FN Plus, BACTEC-Plus and -Lytic in Detection of Anaerobic Bacteria and Identification by Direct MALDI-TOF MS
Detection and identification of anaerobic bacteria in blood cultures (BC) is a well-recognized challenge in clinical microbiology. We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences)...
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| Veröffentlicht in: | PloS one 2015-11, Vol.10 (11), p.e0142398-e0142398 |
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| creator | Almuhayawi, Mohammed Altun, Osman Abdulmajeed, Adam Dilshad Ullberg, Måns Özenci, Volkan |
| description | Detection and identification of anaerobic bacteria in blood cultures (BC) is a well-recognized challenge in clinical microbiology. We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria. BACTEC Lytic had higher detection rate (94/100, 94%) than BacT/ALERT FN Plus (80/100, 80%) (p |
| doi_str_mv | 10.1371/journal.pone.0142398 |
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We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria. BACTEC Lytic had higher detection rate (94/100, 94%) than BacT/ALERT FN Plus (80/100, 80%) (p<0.01) in the studied material. There was no significant difference in detection of anaerobic bacteria among the remaining bottle types. The 67 anaerobic bacteria that signalled positive in all four bottle types were analyzed to compare the time to detection (TTD) and isolates were directly identified by MALDI-TOF MS. There was a significant difference in TTD among the four bottle types (p<0.0001). The shortest median TTD was 18 h in BACTEC Lytic followed by BacT/ALERT FN (23.5 h), BACTEC Plus (27 h) and finally BacT/ALERT FN Plus (38 h) bottles. In contrast, MALDI-TOF MS performed similarly in all bottle types with accurate identification in 51/67 (76%) BacT/ALERT FN, 51/67 (76%) BacT/ALERT FN Plus, 53/67 (79%) BACTEC Plus and 50/67 (75%) BACTEC Lytic bottles. In conclusion, BACTEC Lytic bottles have significantly better detection rates and shorter TTD compared to the three other bottle types. The anaerobic BC bottles are equally suitable for direct MALDI-TOF MS for rapid and reliable identification of common anaerobic bacteria. Further clinical studies are warranted to investigate the performance of anaerobic BC bottles in detection of anaerobic bacteria and identification by direct MALDI-TOF MS.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0142398</identifier><identifier>PMID: 26554930</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anaerobic bacteria ; Bacteria ; Bacteria, Anaerobic - classification ; Bacteria, Anaerobic - growth & development ; Bacteria, Anaerobic - isolation & purification ; Bacteroides ; Blood ; Blood culture ; Blood serum ; Bottles ; Clinical microbiology ; Clostridium ; Desorption ; Humans ; Identification and classification ; Ions ; Laboratories ; Lasers ; Mass spectrometry ; Medicin och hälsovetenskap ; Methods ; Microbiology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Testing</subject><ispartof>PloS one, 2015-11, Vol.10 (11), p.e0142398-e0142398</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Almuhayawi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Almuhayawi et al 2015 Almuhayawi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c846t-900011992dcb0f4e0bae944674cf6a088365752a96c888a1d7858cc3e87057913</citedby><cites>FETCH-LOGICAL-c846t-900011992dcb0f4e0bae944674cf6a088365752a96c888a1d7858cc3e87057913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640713/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640713/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26554930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:132402875$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Woo, Patrick CY</contributor><creatorcontrib>Almuhayawi, Mohammed</creatorcontrib><creatorcontrib>Altun, Osman</creatorcontrib><creatorcontrib>Abdulmajeed, Adam Dilshad</creatorcontrib><creatorcontrib>Ullberg, Måns</creatorcontrib><creatorcontrib>Özenci, Volkan</creatorcontrib><title>The Performance of the Four Anaerobic Blood Culture Bottles BacT/ALERT-FN, -FN Plus, BACTEC-Plus and -Lytic in Detection of Anaerobic Bacteria and Identification by Direct MALDI-TOF MS</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Detection and identification of anaerobic bacteria in blood cultures (BC) is a well-recognized challenge in clinical microbiology. We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria. BACTEC Lytic had higher detection rate (94/100, 94%) than BacT/ALERT FN Plus (80/100, 80%) (p<0.01) in the studied material. There was no significant difference in detection of anaerobic bacteria among the remaining bottle types. The 67 anaerobic bacteria that signalled positive in all four bottle types were analyzed to compare the time to detection (TTD) and isolates were directly identified by MALDI-TOF MS. There was a significant difference in TTD among the four bottle types (p<0.0001). The shortest median TTD was 18 h in BACTEC Lytic followed by BacT/ALERT FN (23.5 h), BACTEC Plus (27 h) and finally BacT/ALERT FN Plus (38 h) bottles. In contrast, MALDI-TOF MS performed similarly in all bottle types with accurate identification in 51/67 (76%) BacT/ALERT FN, 51/67 (76%) BacT/ALERT FN Plus, 53/67 (79%) BACTEC Plus and 50/67 (75%) BACTEC Lytic bottles. In conclusion, BACTEC Lytic bottles have significantly better detection rates and shorter TTD compared to the three other bottle types. The anaerobic BC bottles are equally suitable for direct MALDI-TOF MS for rapid and reliable identification of common anaerobic bacteria. Further clinical studies are warranted to investigate the performance of anaerobic BC bottles in detection of anaerobic bacteria and identification by direct MALDI-TOF MS.</description><subject>Anaerobic bacteria</subject><subject>Bacteria</subject><subject>Bacteria, Anaerobic - classification</subject><subject>Bacteria, Anaerobic - growth & development</subject><subject>Bacteria, Anaerobic - isolation & purification</subject><subject>Bacteroides</subject><subject>Blood</subject><subject>Blood culture</subject><subject>Blood serum</subject><subject>Bottles</subject><subject>Clinical microbiology</subject><subject>Clostridium</subject><subject>Desorption</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Ions</subject><subject>Laboratories</subject><subject>Lasers</subject><subject>Mass spectrometry</subject><subject>Medicin och hälsovetenskap</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - 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We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria. BACTEC Lytic had higher detection rate (94/100, 94%) than BacT/ALERT FN Plus (80/100, 80%) (p<0.01) in the studied material. There was no significant difference in detection of anaerobic bacteria among the remaining bottle types. The 67 anaerobic bacteria that signalled positive in all four bottle types were analyzed to compare the time to detection (TTD) and isolates were directly identified by MALDI-TOF MS. There was a significant difference in TTD among the four bottle types (p<0.0001). The shortest median TTD was 18 h in BACTEC Lytic followed by BacT/ALERT FN (23.5 h), BACTEC Plus (27 h) and finally BacT/ALERT FN Plus (38 h) bottles. In contrast, MALDI-TOF MS performed similarly in all bottle types with accurate identification in 51/67 (76%) BacT/ALERT FN, 51/67 (76%) BacT/ALERT FN Plus, 53/67 (79%) BACTEC Plus and 50/67 (75%) BACTEC Lytic bottles. In conclusion, BACTEC Lytic bottles have significantly better detection rates and shorter TTD compared to the three other bottle types. The anaerobic BC bottles are equally suitable for direct MALDI-TOF MS for rapid and reliable identification of common anaerobic bacteria. Further clinical studies are warranted to investigate the performance of anaerobic BC bottles in detection of anaerobic bacteria and identification by direct MALDI-TOF MS.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26554930</pmid><doi>10.1371/journal.pone.0142398</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Anaerobic bacteria Bacteria Bacteria, Anaerobic - classification Bacteria, Anaerobic - growth & development Bacteria, Anaerobic - isolation & purification Bacteroides Blood Blood culture Blood serum Bottles Clinical microbiology Clostridium Desorption Humans Identification and classification Ions Laboratories Lasers Mass spectrometry Medicin och hälsovetenskap Methods Microbiology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Testing |
| title | The Performance of the Four Anaerobic Blood Culture Bottles BacT/ALERT-FN, -FN Plus, BACTEC-Plus and -Lytic in Detection of Anaerobic Bacteria and Identification by Direct MALDI-TOF MS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A09%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Performance%20of%20the%20Four%20Anaerobic%20Blood%20Culture%20Bottles%20BacT/ALERT-FN,%20-FN%20Plus,%20BACTEC-Plus%20and%20-Lytic%20in%20Detection%20of%20Anaerobic%20Bacteria%20and%20Identification%20by%20Direct%20MALDI-TOF%20MS&rft.jtitle=PloS%20one&rft.au=Almuhayawi,%20Mohammed&rft.date=2015-11-10&rft.volume=10&rft.issue=11&rft.spage=e0142398&rft.epage=e0142398&rft.pages=e0142398-e0142398&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0142398&rft_dat=%3Cgale_plos_%3EA434196708%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1732311842&rft_id=info:pmid/26554930&rft_galeid=A434196708&rft_doaj_id=oai_doaj_org_article_a2b79cf70e1c4e9491ab5401b14b80d6&rfr_iscdi=true |