Establishment of Green Fluorescent Protein and Firefly Luciferase Expressing Mouse Primary Macrophages for In Vivo Bioluminescence Imaging

Macrophages play a key role in tissue homeostasis as well as in a range of pathological conditions including atherosclerosis, cancer, and autoimmunity. Many aspects of their in vivo behavior are, however, poorly understood. Bioluminescence imaging (BLI) with green fluorescent protein (GFP) and firef...

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Veröffentlicht in:	PloS one 2015-11, Vol.10 (11), p.e0142736-e0142736
Hauptverfasser:	Pajarinen, Jukka, Lin, Tzu-Hua, Sato, Taishi, Loi, Florence, Yao, Zhenyu, Konttinen, Yrjö T, Goodman, Stuart B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibiotics Arteriosclerosis Atherosclerosis Autoimmunity Binding sites Biocompatibility Bioluminescence Biomaterials Biomedical materials Blood Bone marrow Cancer Cells, Cultured Cyclosporine - pharmacology Cyclosporins Dendritic cells Dextrans - pharmacology Femur Flow cytometry Fluorescence Gene expression Gene transfer Genetic aspects Genetic research Green fluorescent protein Green fluorescent proteins Green Fluorescent Proteins - genetics Hexadimethrine Bromide - pharmacology Homeostasis Homing Homing behavior Infections Laboratories Luciferase Luciferases, Firefly - genetics Luminescence Lungs Macrophages Macrophages - cytology Macrophages - drug effects Macrophages - metabolism Male Medicine Mice Mice, Inbred BALB C Molecular Imaging Osteolysis Precision medicine Properties Proteins Surgery Transduction, Genetic
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