Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin
The skin is a rich source of readily accessible stem cells. The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolatio...
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| creator | Forni, Maria Fernanda Ramos Maia Lobba, Aline Pereira Ferreira, Alexandre Hamilton Sogayar, Mari Cleide |
| description | The skin is a rich source of readily accessible stem cells. The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolation from skin; however, none of them afforded simultaneous isolation of more than one population. Herein, we describe the simultaneous isolation and characterization of three stem cell populations from the dermis and epidermis of murine skin, namely Epidermal Stem Cells (EpiSCs), Skin-derived Precursors (SKPs) and Mesenchymal Stem Cells (MSCs). The simultaneous isolation was possible through a simple protocol based on culture selection techniques. These cell populations are shown to be capable of generating chondrocytes, adipocytes, osteocytes, terminally differentiated keratinocytes, neurons and glia, rendering this protocol suitable for the isolation of cells for tissue replenishment and cell based therapies. The advantages of this procedure are far-reaching since the skin is not only the largest organ in the body, but also provides an easily accessible source of stem cells for autologous graft. |
| doi_str_mv | 10.1371/journal.pone.0140143 |
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The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolation from skin; however, none of them afforded simultaneous isolation of more than one population. Herein, we describe the simultaneous isolation and characterization of three stem cell populations from the dermis and epidermis of murine skin, namely Epidermal Stem Cells (EpiSCs), Skin-derived Precursors (SKPs) and Mesenchymal Stem Cells (MSCs). The simultaneous isolation was possible through a simple protocol based on culture selection techniques. These cell populations are shown to be capable of generating chondrocytes, adipocytes, osteocytes, terminally differentiated keratinocytes, neurons and glia, rendering this protocol suitable for the isolation of cells for tissue replenishment and cell based therapies. The advantages of this procedure are far-reaching since the skin is not only the largest organ in the body, but also provides an easily accessible source of stem cells for autologous graft.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0140143</identifier><identifier>PMID: 26462205</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism ; Accessibility ; Adipocytes ; Adipocytes - cytology ; Adipocytes - drug effects ; Animals ; Antigens, CD - metabolism ; Autografts ; Biocompatibility ; Biology ; Biomedical materials ; Cell Adhesion - drug effects ; Cell culture ; Cell cycle ; Cell Differentiation - drug effects ; Cell Separation - methods ; Chondrocytes ; Chondrocytes - cytology ; Chondrocytes - drug effects ; Collagen Type IV - pharmacology ; Dehydration (Physiology) ; Dermis ; Dermis - cytology ; Epidermis ; Epidermis - cytology ; Euthanasia ; Genetic aspects ; Genomes ; Glial Fibrillary Acidic Protein - metabolism ; Hair ; Keratinocytes ; Male ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; Mice, Inbred BALB C ; Neuronal-glial interactions ; Osteocytes ; Osteocytes - cytology ; Osteocytes - drug effects ; Physiological aspects ; Population ; Populations ; Real-Time Polymerase Chain Reaction ; Regenerative medicine ; Replenishment ; Skin ; Skin - cytology ; Stem cell transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Tissue engineering ; Tubulin - metabolism</subject><ispartof>PloS one, 2015-10, Vol.10 (10), p.e0140143-e0140143</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Forni et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Forni et al 2015 Forni et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-62e62e29330912eea04fd17fde1a69393b21cd6392308fa7db2511a27703c3193</citedby><cites>FETCH-LOGICAL-c758t-62e62e29330912eea04fd17fde1a69393b21cd6392308fa7db2511a27703c3193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604199/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604199/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26462205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Eckert, Richard L.</contributor><creatorcontrib>Forni, Maria Fernanda</creatorcontrib><creatorcontrib>Ramos Maia Lobba, Aline</creatorcontrib><creatorcontrib>Pereira Ferreira, Alexandre Hamilton</creatorcontrib><creatorcontrib>Sogayar, Mari Cleide</creatorcontrib><title>Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The skin is a rich source of readily accessible stem cells. 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The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolation from skin; however, none of them afforded simultaneous isolation of more than one population. Herein, we describe the simultaneous isolation and characterization of three stem cell populations from the dermis and epidermis of murine skin, namely Epidermal Stem Cells (EpiSCs), Skin-derived Precursors (SKPs) and Mesenchymal Stem Cells (MSCs). The simultaneous isolation was possible through a simple protocol based on culture selection techniques. These cell populations are shown to be capable of generating chondrocytes, adipocytes, osteocytes, terminally differentiated keratinocytes, neurons and glia, rendering this protocol suitable for the isolation of cells for tissue replenishment and cell based therapies. The advantages of this procedure are far-reaching since the skin is not only the largest organ in the body, but also provides an easily accessible source of stem cells for autologous graft.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26462205</pmid><doi>10.1371/journal.pone.0140143</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 2',3'-Cyclic-Nucleotide Phosphodiesterases - metabolism Accessibility Adipocytes Adipocytes - cytology Adipocytes - drug effects Animals Antigens, CD - metabolism Autografts Biocompatibility Biology Biomedical materials Cell Adhesion - drug effects Cell culture Cell cycle Cell Differentiation - drug effects Cell Separation - methods Chondrocytes Chondrocytes - cytology Chondrocytes - drug effects Collagen Type IV - pharmacology Dehydration (Physiology) Dermis Dermis - cytology Epidermis Epidermis - cytology Euthanasia Genetic aspects Genomes Glial Fibrillary Acidic Protein - metabolism Hair Keratinocytes Male Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchyme Mice, Inbred BALB C Neuronal-glial interactions Osteocytes Osteocytes - cytology Osteocytes - drug effects Physiological aspects Population Populations Real-Time Polymerase Chain Reaction Regenerative medicine Replenishment Skin Skin - cytology Stem cell transplantation Stem cells Stem Cells - cytology Stem Cells - drug effects Tissue engineering Tubulin - metabolism |
| title | Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin |
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