Differential Regulation of Gene and Protein Expression by Zinc Oxide Nanoparticles in Hen's Ovarian Granulosa Cells: Specific Roles of Nanoparticles
Annually, tons and tons of zinc oxide nanoparticles (ZnO NPs) are produced in the world. And they are applied in almost all aspects of our life. Their release from the products into environment may pose issue for human health. Although many studies have reported the adverse effects of ZnO NPs on org...
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| creator | Zhao, Yong Li, Lan Zhang, Peng-Fei Shen, Wei Liu, Jing Yang, Fen-Fang Liu, Hong-Bo Hao, Zhi-Hui |
| description | Annually, tons and tons of zinc oxide nanoparticles (ZnO NPs) are produced in the world. And they are applied in almost all aspects of our life. Their release from the products into environment may pose issue for human health. Although many studies have reported the adverse effects of ZnO NPs on organisms, little is known about the effects on female reproductive systems or the related mechanisms. Quantitative proteomics have not been applied although quantitative transcriptomics have been used in zinc oxide nanoparticles (ZnO NPs) research. Genes are very important players however proteins are the real actors in the biological systems. By using hen's ovarian granulosa cells, it was found that ZnO-NP-5μg/ml and ZnSO4-10μg/ml treatments produced the same amount of intracellular Zn and resulted in similar cell growth inhibition. And NPs were found in the treated cells. However, ZnO-NP-5μg/ml specifically regulated the expression of genes and proteins compared with that in ZnSO4-10μg/ml treatment. For the first time, this investigation reports that intact NPs produce different impacts on the expression of genes and proteins involved in specific pathways compared to that by Zn2+. The findings enrich our knowledge for the molecular insights of zinc oxide nanoparticles effects on the female reproductive systems. This also may raise the health concern that ZnO NPs may adversely affect the female reproductive systems through regulation of specific signaling pathways. |
| doi_str_mv | 10.1371/journal.pone.0140499 |
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And they are applied in almost all aspects of our life. Their release from the products into environment may pose issue for human health. Although many studies have reported the adverse effects of ZnO NPs on organisms, little is known about the effects on female reproductive systems or the related mechanisms. Quantitative proteomics have not been applied although quantitative transcriptomics have been used in zinc oxide nanoparticles (ZnO NPs) research. Genes are very important players however proteins are the real actors in the biological systems. By using hen's ovarian granulosa cells, it was found that ZnO-NP-5μg/ml and ZnSO4-10μg/ml treatments produced the same amount of intracellular Zn and resulted in similar cell growth inhibition. And NPs were found in the treated cells. However, ZnO-NP-5μg/ml specifically regulated the expression of genes and proteins compared with that in ZnSO4-10μg/ml treatment. For the first time, this investigation reports that intact NPs produce different impacts on the expression of genes and proteins involved in specific pathways compared to that by Zn2+. The findings enrich our knowledge for the molecular insights of zinc oxide nanoparticles effects on the female reproductive systems. This also may raise the health concern that ZnO NPs may adversely affect the female reproductive systems through regulation of specific signaling pathways.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0140499</identifier><identifier>PMID: 26460738</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal reproduction ; Animals ; Avian Proteins - genetics ; Avian Proteins - metabolism ; Blotting, Western ; Cell Cycle - drug effects ; Cell Proliferation - drug effects ; Chickens - genetics ; Daphnia magna ; Embryos ; Female ; Gene expression ; Gene Expression Regulation - drug effects ; Genes ; Genomes ; Germplasm ; Granulosa cells ; Granulosa Cells - cytology ; Granulosa Cells - drug effects ; Granulosa Cells - metabolism ; Isotope Labeling ; Laboratories ; Mammals ; Nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - toxicity ; Nanoparticles - ultrastructure ; Ontology ; Pathways ; Pharmaceutical sciences ; Physiological aspects ; Poultry ; Protein expression ; Proteins ; Proteomics ; Quantum dots ; Reproductive system ; Reproductive systems ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Zebrafish ; Zinc ; Zinc oxide ; Zinc Oxide - toxicity ; Zinc oxides ; Zinc Sulfate - pharmacology</subject><ispartof>PloS one, 2015-10, Vol.10 (10), p.e0140499-e0140499</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Zhao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Zhao et al 2015 Zhao et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a4f16b72e94c4040fdf66d223c27b4fc4b8ddeb45898f0cd1635cc01a6ad81053</citedby><cites>FETCH-LOGICAL-c692t-a4f16b72e94c4040fdf66d223c27b4fc4b8ddeb45898f0cd1635cc01a6ad81053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604165/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4604165/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26460738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mishra, Yogendra Kumar</contributor><creatorcontrib>Zhao, Yong</creatorcontrib><creatorcontrib>Li, Lan</creatorcontrib><creatorcontrib>Zhang, Peng-Fei</creatorcontrib><creatorcontrib>Shen, Wei</creatorcontrib><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Yang, Fen-Fang</creatorcontrib><creatorcontrib>Liu, Hong-Bo</creatorcontrib><creatorcontrib>Hao, Zhi-Hui</creatorcontrib><title>Differential Regulation of Gene and Protein Expression by Zinc Oxide Nanoparticles in Hen's Ovarian Granulosa Cells: Specific Roles of Nanoparticles</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Annually, tons and tons of zinc oxide nanoparticles (ZnO NPs) are produced in the world. 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For the first time, this investigation reports that intact NPs produce different impacts on the expression of genes and proteins involved in specific pathways compared to that by Zn2+. The findings enrich our knowledge for the molecular insights of zinc oxide nanoparticles effects on the female reproductive systems. This also may raise the health concern that ZnO NPs may adversely affect the female reproductive systems through regulation of specific signaling pathways.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>Avian Proteins - genetics</subject><subject>Avian Proteins - metabolism</subject><subject>Blotting, Western</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Chickens - genetics</subject><subject>Daphnia magna</subject><subject>Embryos</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes</subject><subject>Genomes</subject><subject>Germplasm</subject><subject>Granulosa cells</subject><subject>Granulosa Cells - cytology</subject><subject>Granulosa Cells - drug effects</subject><subject>Granulosa Cells - metabolism</subject><subject>Isotope Labeling</subject><subject>Laboratories</subject><subject>Mammals</subject><subject>Nanoparticles</subject><subject>Nanoparticles - 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And they are applied in almost all aspects of our life. Their release from the products into environment may pose issue for human health. Although many studies have reported the adverse effects of ZnO NPs on organisms, little is known about the effects on female reproductive systems or the related mechanisms. Quantitative proteomics have not been applied although quantitative transcriptomics have been used in zinc oxide nanoparticles (ZnO NPs) research. Genes are very important players however proteins are the real actors in the biological systems. By using hen's ovarian granulosa cells, it was found that ZnO-NP-5μg/ml and ZnSO4-10μg/ml treatments produced the same amount of intracellular Zn and resulted in similar cell growth inhibition. And NPs were found in the treated cells. However, ZnO-NP-5μg/ml specifically regulated the expression of genes and proteins compared with that in ZnSO4-10μg/ml treatment. For the first time, this investigation reports that intact NPs produce different impacts on the expression of genes and proteins involved in specific pathways compared to that by Zn2+. The findings enrich our knowledge for the molecular insights of zinc oxide nanoparticles effects on the female reproductive systems. This also may raise the health concern that ZnO NPs may adversely affect the female reproductive systems through regulation of specific signaling pathways.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26460738</pmid><doi>10.1371/journal.pone.0140499</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-10, Vol.10 (10), p.e0140499-e0140499 |
| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal reproduction Animals Avian Proteins - genetics Avian Proteins - metabolism Blotting, Western Cell Cycle - drug effects Cell Proliferation - drug effects Chickens - genetics Daphnia magna Embryos Female Gene expression Gene Expression Regulation - drug effects Genes Genomes Germplasm Granulosa cells Granulosa Cells - cytology Granulosa Cells - drug effects Granulosa Cells - metabolism Isotope Labeling Laboratories Mammals Nanoparticles Nanoparticles - chemistry Nanoparticles - toxicity Nanoparticles - ultrastructure Ontology Pathways Pharmaceutical sciences Physiological aspects Poultry Protein expression Proteins Proteomics Quantum dots Reproductive system Reproductive systems RNA, Messenger - genetics RNA, Messenger - metabolism Zebrafish Zinc Zinc oxide Zinc Oxide - toxicity Zinc oxides Zinc Sulfate - pharmacology |
| title | Differential Regulation of Gene and Protein Expression by Zinc Oxide Nanoparticles in Hen's Ovarian Granulosa Cells: Specific Roles of Nanoparticles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T16%3A09%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20Regulation%20of%20Gene%20and%20Protein%20Expression%20by%20Zinc%20Oxide%20Nanoparticles%20in%20Hen's%20Ovarian%20Granulosa%20Cells:%20Specific%20Roles%20of%20Nanoparticles&rft.jtitle=PloS%20one&rft.au=Zhao,%20Yong&rft.date=2015-10-13&rft.volume=10&rft.issue=10&rft.spage=e0140499&rft.epage=e0140499&rft.pages=e0140499-e0140499&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0140499&rft_dat=%3Cgale_plos_%3EA431491219%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1721911168&rft_id=info:pmid/26460738&rft_galeid=A431491219&rft_doaj_id=oai_doaj_org_article_90da33d9cf094202899d12de833856ec&rfr_iscdi=true |