Trans-Reactivation: A New Epigenetic Phenomenon Underlying Transcriptional Reactivation of Silenced Genes
In order to study the role played by cellular RNA pools produced by homologous genomic loci in defining the transcriptional state of a silenced gene, we tested the effect of non-functional alleles of the white gene in the presence of a functional copy of white, silenced by heterochromatin. We found...
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| Veröffentlicht in: | PLoS genetics 2015-08, Vol.11 (8), p.e1005444-e1005444 |
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| creator | Onorati, Maria Cristina Arancio, Walter Cavalieri, Vincenzo Ingrassia, Antonia M R Pavesi, Giulio Corona, Davide F V |
| description | In order to study the role played by cellular RNA pools produced by homologous genomic loci in defining the transcriptional state of a silenced gene, we tested the effect of non-functional alleles of the white gene in the presence of a functional copy of white, silenced by heterochromatin. We found that non-functional alleles of white, unable to produce a coding transcript, could reactivate in trans the expression of a wild type copy of the same gene silenced by heterochromatin. This new epigenetic phenomenon of transcriptional trans-reactivation is heritable, relies on the presence of homologous RNA's and is affected by mutations in genes involved in post-transcriptional gene silencing. Our data suggest a general new unexpected level of gene expression control mediated by homologous RNA molecules in the context of heterochromatic genes. |
| doi_str_mv | 10.1371/journal.pgen.1005444 |
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We found that non-functional alleles of white, unable to produce a coding transcript, could reactivate in trans the expression of a wild type copy of the same gene silenced by heterochromatin. This new epigenetic phenomenon of transcriptional trans-reactivation is heritable, relies on the presence of homologous RNA's and is affected by mutations in genes involved in post-transcriptional gene silencing. 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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Onorati MC, Arancio W, Cavalieri V, Ingrassia AMR, Pavesi G, Corona DFV (2015) Trans-Reactivation: A New Epigenetic Phenomenon Underlying Transcriptional Reactivation of Silenced Genes. 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Our data suggest a general new unexpected level of gene expression control mediated by homologous RNA molecules in the context of heterochromatic genes.</description><subject>Alleles</subject><subject>Animals</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>Chromosomes</subject><subject>Compound Eye, Arthropod - physiology</subject><subject>Drosophila melanogaster</subject><subject>Drosophila Proteins - genetics</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Eye Color - genetics</subject><subject>Eye Proteins - genetics</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Gene loci</subject><subject>Genes, Insect</subject><subject>Genetic transcription</subject><subject>Genomes</subject><subject>Heterochromatin - genetics</subject><subject>Male</subject><subject>Mutation</subject><subject>Observations</subject><subject>RNA Interference</subject><subject>RNA, Untranslated - genetics</subject><subject>Transcription, Genetic</subject><issn>1553-7404</issn><issn>1553-7390</issn><issn>1553-7404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNpVkl9v0zAUxSMEYmPwDRD4CfGS4r9xwgNSNY0xaQIE27N1m9y0rly7s9OhfXucNpu6B8uWfe7P516donjP6IwJzb6swy56cLPtEv2MUaqklC-KU6aUKLWk8uXR-aR4k9KaUqHqRr8uTnjFG84ZPS3sTQSfyj8I7WDvYbDBfyVz8hP_kYutzWgcbEt-r9CHTV6e3PoOo3uwfkn2pW2027EKHDmGkNCTv9ahb7EjlxmT3havenAJ3037WXH7_eLm_Ed5_evy6nx-XbaKN0OpsFc1Aq0Xiw65FH0NtZAANJ_qRumGC6GBKw6yBmgEVBqYpnxRCSEQqTgrPh64WxeSmaaUDNOcVkxyrbPi6qDoAqzNNtoNxAcTwJr9RYhLAzG37dDwhaxE3SJtOiax7hpJUdJO9kr3qpcj69v0226xwa5FP0Rwz6DPX7xdmWW4N1JlshYZ8HkCxHC3wzSYjU0tOgcew270TStOdc1H6aeDdAnZ2grBDasU3G6cdzJzKWiTkXtT8iBsY0gpYv_kh1EzhudxLGYMj5nCk8s-HPfyVPSYFvEfhrXDQw</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Onorati, Maria Cristina</creator><creator>Arancio, Walter</creator><creator>Cavalieri, Vincenzo</creator><creator>Ingrassia, Antonia M R</creator><creator>Pavesi, Giulio</creator><creator>Corona, Davide F V</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150801</creationdate><title>Trans-Reactivation: A New Epigenetic Phenomenon Underlying Transcriptional Reactivation of Silenced Genes</title><author>Onorati, Maria Cristina ; Arancio, Walter ; Cavalieri, Vincenzo ; Ingrassia, Antonia M R ; Pavesi, Giulio ; Corona, Davide F V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-5ef58ea08bbde243f8a834aa03f8895792337a252a48aa93a67a1702b6333ee03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>Chromosomes</topic><topic>Compound Eye, Arthropod - physiology</topic><topic>Drosophila melanogaster</topic><topic>Drosophila Proteins - genetics</topic><topic>Epigenetic inheritance</topic><topic>Epigenetics</topic><topic>Eye Color - genetics</topic><topic>Eye Proteins - genetics</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Gene loci</topic><topic>Genes, Insect</topic><topic>Genetic transcription</topic><topic>Genomes</topic><topic>Heterochromatin - genetics</topic><topic>Male</topic><topic>Mutation</topic><topic>Observations</topic><topic>RNA Interference</topic><topic>RNA, Untranslated - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Onorati, Maria Cristina</creatorcontrib><creatorcontrib>Arancio, Walter</creatorcontrib><creatorcontrib>Cavalieri, Vincenzo</creatorcontrib><creatorcontrib>Ingrassia, Antonia M R</creatorcontrib><creatorcontrib>Pavesi, Giulio</creatorcontrib><creatorcontrib>Corona, Davide F V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Onorati, Maria Cristina</au><au>Arancio, Walter</au><au>Cavalieri, Vincenzo</au><au>Ingrassia, Antonia M R</au><au>Pavesi, Giulio</au><au>Corona, Davide F V</au><au>Pirrotta, Vincenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-Reactivation: A New Epigenetic Phenomenon Underlying Transcriptional Reactivation of Silenced Genes</atitle><jtitle>PLoS genetics</jtitle><addtitle>PLoS Genet</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>11</volume><issue>8</issue><spage>e1005444</spage><epage>e1005444</epage><pages>e1005444-e1005444</pages><issn>1553-7404</issn><issn>1553-7390</issn><eissn>1553-7404</eissn><abstract>In order to study the role played by cellular RNA pools produced by homologous genomic loci in defining the transcriptional state of a silenced gene, we tested the effect of non-functional alleles of the white gene in the presence of a functional copy of white, silenced by heterochromatin. We found that non-functional alleles of white, unable to produce a coding transcript, could reactivate in trans the expression of a wild type copy of the same gene silenced by heterochromatin. This new epigenetic phenomenon of transcriptional trans-reactivation is heritable, relies on the presence of homologous RNA's and is affected by mutations in genes involved in post-transcriptional gene silencing. Our data suggest a general new unexpected level of gene expression control mediated by homologous RNA molecules in the context of heterochromatic genes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26292210</pmid><doi>10.1371/journal.pgen.1005444</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Alleles Animals ATP-Binding Cassette Transporters - genetics Chromosomes Compound Eye, Arthropod - physiology Drosophila melanogaster Drosophila Proteins - genetics Epigenetic inheritance Epigenetics Eye Color - genetics Eye Proteins - genetics Female Females Gene expression Gene loci Genes, Insect Genetic transcription Genomes Heterochromatin - genetics Male Mutation Observations RNA Interference RNA, Untranslated - genetics Transcription, Genetic |
| title | Trans-Reactivation: A New Epigenetic Phenomenon Underlying Transcriptional Reactivation of Silenced Genes |
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