Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients
Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the...
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| creator | Ghosh, Nandini Sircar, Gaurab Saha, Bodhisattwa Pandey, Naren Gupta Bhattacharya, Swati |
| description | Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools.
Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.
Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.
Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy. |
| doi_str_mv | 10.1371/journal.pone.0138992 |
| format | Article |
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Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.
Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.
Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0138992</identifier><identifier>PMID: 26418046</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Allergens ; Allergens - immunology ; Allergens - metabolism ; Allergies ; Allergy ; Ambrosia artemisiifolia ; Antibodies ; Antigens ; Antigens, Plant - immunology ; Antigens, Plant - metabolism ; Artemisia vulgaris ; Asteraceae ; Asthma ; Atopy ; Bioinformatics ; Case-Control Studies ; Causes of ; Cluster analysis ; Clustering ; Cysteine proteinase ; Electrophoresis, Gel, Two-Dimensional ; Female ; Glycan ; Helianthus - immunology ; Helianthus - metabolism ; Helianthus annuus ; Histamine ; Homology ; Humans ; Hypersensitivity ; Hypersensitivity, Immediate - diagnosis ; Hypersensitivity, Immediate - immunology ; Hypersensitivity, Immediate - metabolism ; Identification ; Immunoblotting ; Immunoglobulin E ; Immunoglobulin E - immunology ; Immunoglobulin E - metabolism ; Immunology ; Male ; Mass spectrometry ; Middle Aged ; Molecular weight ; Patients ; Physiological aspects ; Plant biology ; Plant Proteins - immunology ; Plant Proteins - metabolism ; Pollen ; Pollen - immunology ; Pollen - metabolism ; Pollinosis ; Proteins ; Proteome - analysis ; Proteomics ; Proteomics - methods ; Respiratory System - immunology ; Respiratory System - metabolism ; Rhinitis, Allergic, Seasonal - diagnosis ; Rhinitis, Allergic, Seasonal - immunology ; Scientific imaging ; Skin - immunology ; Skin - metabolism ; Spectrometry ; Studies ; Sugar ; Sunflowers ; Tandem Mass Spectrometry ; Test procedures ; Young Adult</subject><ispartof>PloS one, 2015-09, Vol.10 (9), p.e0138992-e0138992</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Ghosh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Ghosh et al 2015 Ghosh et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-d55a87607d4d2125a4fc1d614cf3a8ab98b26065b02fb65a924e4ff8d10ec4a33</citedby><cites>FETCH-LOGICAL-c758t-d55a87607d4d2125a4fc1d614cf3a8ab98b26065b02fb65a924e4ff8d10ec4a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587886/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4587886/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26418046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Wei</contributor><creatorcontrib>Ghosh, Nandini</creatorcontrib><creatorcontrib>Sircar, Gaurab</creatorcontrib><creatorcontrib>Saha, Bodhisattwa</creatorcontrib><creatorcontrib>Pandey, Naren</creatorcontrib><creatorcontrib>Gupta Bhattacharya, Swati</creatorcontrib><title>Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools.
Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.
Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.
Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Allergens</subject><subject>Allergens - immunology</subject><subject>Allergens - metabolism</subject><subject>Allergies</subject><subject>Allergy</subject><subject>Ambrosia artemisiifolia</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antigens, Plant - immunology</subject><subject>Antigens, Plant - metabolism</subject><subject>Artemisia vulgaris</subject><subject>Asteraceae</subject><subject>Asthma</subject><subject>Atopy</subject><subject>Bioinformatics</subject><subject>Case-Control Studies</subject><subject>Causes of</subject><subject>Cluster analysis</subject><subject>Clustering</subject><subject>Cysteine proteinase</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>Glycan</subject><subject>Helianthus - immunology</subject><subject>Helianthus - metabolism</subject><subject>Helianthus annuus</subject><subject>Histamine</subject><subject>Homology</subject><subject>Humans</subject><subject>Hypersensitivity</subject><subject>Hypersensitivity, Immediate - diagnosis</subject><subject>Hypersensitivity, Immediate - immunology</subject><subject>Hypersensitivity, Immediate - metabolism</subject><subject>Identification</subject><subject>Immunoblotting</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunoglobulin E - metabolism</subject><subject>Immunology</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Middle Aged</subject><subject>Molecular weight</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Plant biology</subject><subject>Plant Proteins - immunology</subject><subject>Plant Proteins - metabolism</subject><subject>Pollen</subject><subject>Pollen - immunology</subject><subject>Pollen - metabolism</subject><subject>Pollinosis</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Respiratory System - immunology</subject><subject>Respiratory System - metabolism</subject><subject>Rhinitis, Allergic, Seasonal - diagnosis</subject><subject>Rhinitis, Allergic, Seasonal - immunology</subject><subject>Scientific imaging</subject><subject>Skin - immunology</subject><subject>Skin - metabolism</subject><subject>Spectrometry</subject><subject>Studies</subject><subject>Sugar</subject><subject>Sunflowers</subject><subject>Tandem Mass Spectrometry</subject><subject>Test procedures</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk81u1DAQgCMEolB4AwSWkFB72MV_cRwOSKsKaKWiVl3gajmOveuV115sBygPwHPjbdOqi3pAOTiafPNNZpKpqhcIThFp0NtVGKKXbroJXk8hIrxt8YPqCWoJnjAMycM793vV05RWENaEM_a42sOMIg4pe1L9mWsZ1RKYEMHMOR0X2idgYliDvNTgPJSYB-cxZB3WGgQD5oM3LvzUERwca2elz8shAen9UI7T6eE7MAOf5ar45kVls_1d0K3-QqeNjTKHeDmWugTnMlvtc3pWPTLSJf18PPerrx8_fDk6npyefTo5mp1OVFPzPOnrWvKGwaanPUa4ltQo1DNElSGSy67lHWaQ1R3EpmO1bDHV1BjeI6gVlYTsV6-uvRsXkhhHmARqtqNChLSFOLkm-iBXYhPtWsZLEaQVV4EQF0LGbJXTQkLc1hq2jeKMypZ1hhulekm6Fjc1osX1fqw2dGvdq9JplG5HuvvE26VYhB-C1rzhnBXBwSiI4fugUxZrm5R2Tnodhqv35oQghrfo63_Q-7sbqYUsDVhvQqmrtlIxowRiUjMOCzW9hypXr9dWlf_N2BLfSTjcSShM1r_yQg4piZP5xf-zZ9922Td32KWWLi9TcEO2waddkF6DKoaUoja3Q0ZQbNflZhpiuy5iXJeS9vLuB7pNutkP8hftXBAp</recordid><startdate>20150929</startdate><enddate>20150929</enddate><creator>Ghosh, Nandini</creator><creator>Sircar, Gaurab</creator><creator>Saha, Bodhisattwa</creator><creator>Pandey, Naren</creator><creator>Gupta Bhattacharya, Swati</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150929</creationdate><title>Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients</title><author>Ghosh, Nandini ; Sircar, Gaurab ; Saha, Bodhisattwa ; Pandey, Naren ; Gupta Bhattacharya, Swati</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-d55a87607d4d2125a4fc1d614cf3a8ab98b26065b02fb65a924e4ff8d10ec4a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Allergens</topic><topic>Allergens - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghosh, Nandini</au><au>Sircar, Gaurab</au><au>Saha, Bodhisattwa</au><au>Pandey, Naren</au><au>Gupta Bhattacharya, Swati</au><au>Wang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-09-29</date><risdate>2015</risdate><volume>10</volume><issue>9</issue><spage>e0138992</spage><epage>e0138992</epage><pages>e0138992-e0138992</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools.
Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensional electrophoretic separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoallergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry.
Prevalence of sunflower pollen sensitization was observed among 21% of the pollen allergic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient sera detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two pectate lyases and a cysteine protease.
Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26418046</pmid><doi>10.1371/journal.pone.0138992</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-09, Vol.10 (9), p.e0138992-e0138992 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1719321339 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Allergens Allergens - immunology Allergens - metabolism Allergies Allergy Ambrosia artemisiifolia Antibodies Antigens Antigens, Plant - immunology Antigens, Plant - metabolism Artemisia vulgaris Asteraceae Asthma Atopy Bioinformatics Case-Control Studies Causes of Cluster analysis Clustering Cysteine proteinase Electrophoresis, Gel, Two-Dimensional Female Glycan Helianthus - immunology Helianthus - metabolism Helianthus annuus Histamine Homology Humans Hypersensitivity Hypersensitivity, Immediate - diagnosis Hypersensitivity, Immediate - immunology Hypersensitivity, Immediate - metabolism Identification Immunoblotting Immunoglobulin E Immunoglobulin E - immunology Immunoglobulin E - metabolism Immunology Male Mass spectrometry Middle Aged Molecular weight Patients Physiological aspects Plant biology Plant Proteins - immunology Plant Proteins - metabolism Pollen Pollen - immunology Pollen - metabolism Pollinosis Proteins Proteome - analysis Proteomics Proteomics - methods Respiratory System - immunology Respiratory System - metabolism Rhinitis, Allergic, Seasonal - diagnosis Rhinitis, Allergic, Seasonal - immunology Scientific imaging Skin - immunology Skin - metabolism Spectrometry Studies Sugar Sunflowers Tandem Mass Spectrometry Test procedures Young Adult |
| title | Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T21%3A12%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Search%20for%20Allergens%20from%20the%20Pollen%20Proteome%20of%20Sunflower%20(Helianthus%20annuus%20L.):%20A%20Major%20Sensitizer%20for%20Respiratory%20Allergy%20Patients&rft.jtitle=PloS%20one&rft.au=Ghosh,%20Nandini&rft.date=2015-09-29&rft.volume=10&rft.issue=9&rft.spage=e0138992&rft.epage=e0138992&rft.pages=e0138992-e0138992&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0138992&rft_dat=%3Cgale_plos_%3EA430235680%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1719321339&rft_id=info:pmid/26418046&rft_galeid=A430235680&rft_doaj_id=oai_doaj_org_article_a0295e097c864a96bf8fccda3b927514&rfr_iscdi=true |