Plaque2.0-A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion

Classical plaque assay measures the propagation of infectious agents across a monolayer of cells. It is dependent on cell lysis, and limited by user-specific settings and low throughput. Here, we developed Plaque2.0, a broadly applicable, fluorescence microscopy-based high-throughput method to mine...

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Veröffentlicht in:	PloS one 2015-09, Vol.10 (9), p.e0138760-e0138760
Hauptverfasser:	Yakimovich, Artur, Andriasyan, Vardan, Witte, Robert, Wang, I-Hsuan, Prasad, Vibhu, Suomalainen, Maarit, Greber, Urs F
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - physiology Animals Cancer Cell adhesion & migration Cell Count Cell Line Cell migration Cell Proliferation Clone Cells Coculture Techniques Deoxyribonucleic acid Diagnosis Disease transmission DNA DNA viruses Feature extraction Fluorescence Fluorescence microscopy Genotype High-Throughput Screening Assays - methods Histochemistry Humans In Situ Hybridization, Fluorescence Infections Information processing Kinases Lysis Metastases Microscopy, Fluorescence Neoplasms - pathology Phenotype Physiological aspects Picornaviridae Infections - virology Plaque assay Population levels Rhinovirus Rhinovirus - genetics Ribonucleic acid RNA RNA viruses Software Time-Lapse Imaging Vaccinia virus Viral Plaque Assay - methods Virus diseases Virus replication Viruses Viruses - metabolism
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