Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification

Dengue is usually diagnosed by isolation of the virus, serology or molecular diagnostic methods. Several commercial kits for the diagnosis of dengue are existing, but concerns have arisen regarding to the affordability and performance characteristics of these kits. Hence, the loop-mediated isotherma...

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Veröffentlicht in:	PloS one 2015-09, Vol.10 (9), p.e0138694-e0138694
Hauptverfasser:	Lau, Yee-Ling, Lai, Meng-Yee, Teoh, Boon-Teong, Abd-Jamil, Juraina, Johari, Jefree, Sam, Sing-Sin, Tan, Kim-Kee, AbuBakar, Sazaly
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Case-Control Studies Chikungunya virus Colorimetry Colorimetry - methods Dengue Dengue - blood Dengue - diagnosis Dengue fever Deoxyribonucleic acid Development and progression Diagnosis Diagnostic systems Diagnostic tests DNA Education Encephalitis Enzyme-linked immunosorbent assay Ethics Gene sequencing Genetic aspects Humans Immunoglobulins Infections Infectious diseases Medicine Methods Nucleic Acid Amplification Techniques - methods Optimization Parasitology Patient outcomes Patients Ribonucleic acid RNA RNA viruses Sensitivity and Specificity Serology Serotypes Surveillance Transcription (Genetics) Turbidity Vector-borne diseases Viral diseases Viruses West Nile virus
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