Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells
Laminin isoforms laminin-511 and -521 are expressed by human embryonic stem cells (hESC) and can be used as a growth matrix to culture these cells under pluripotent conditions. However, the expression of these laminins during the induction of hESC differentiation has not been studied in detail. Furt...
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| creator | Pook, Martin Teino, Indrek Kallas, Ade Maimets, Toivo Ingerpuu, Sulev Jaks, Viljar |
| description | Laminin isoforms laminin-511 and -521 are expressed by human embryonic stem cells (hESC) and can be used as a growth matrix to culture these cells under pluripotent conditions. However, the expression of these laminins during the induction of hESC differentiation has not been studied in detail. Furthermore, the data regarding the expression pattern of laminin chains in differentiating hESC is scarce. In the current study we aimed to fill this gap and investigated the potential changes in laminin expression during early hESC differentiation induced by retinoic acid (RA). We found that laminin-511 but not -521 accumulates in the committed cells during early steps of hESC differentiation. We also performed a comprehensive analysis of the laminin chain repertoire and found that pluripotent hESC express a more diverse range of laminin chains than shown previously. In particular, we provide the evidence that in addition to α1, α5, β1, β2 and γ1 chains, hESC express α2, α3, β3, γ2 and γ3 chain proteins and mRNA. Additionally, we found that a variant of laminin α3 chain-145 kDa-accumulated in RA-treated hESC showing that these cells produce prevalently specifically modified version of α3 chain in early phase of differentiation. |
| doi_str_mv | 10.1371/journal.pone.0138346 |
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However, the expression of these laminins during the induction of hESC differentiation has not been studied in detail. Furthermore, the data regarding the expression pattern of laminin chains in differentiating hESC is scarce. In the current study we aimed to fill this gap and investigated the potential changes in laminin expression during early hESC differentiation induced by retinoic acid (RA). We found that laminin-511 but not -521 accumulates in the committed cells during early steps of hESC differentiation. We also performed a comprehensive analysis of the laminin chain repertoire and found that pluripotent hESC express a more diverse range of laminin chains than shown previously. In particular, we provide the evidence that in addition to α1, α5, β1, β2 and γ1 chains, hESC express α2, α3, β3, γ2 and γ3 chain proteins and mRNA. Additionally, we found that a variant of laminin α3 chain-145 kDa-accumulated in RA-treated hESC showing that these cells produce prevalently specifically modified version of α3 chain in early phase of differentiation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0138346</identifier><identifier>PMID: 26378917</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Binding sites ; Biology ; Cell adhesion & migration ; Cell culture ; Cell Differentiation - physiology ; Cells, Cultured ; Chains ; Differentiation ; Embryo cells ; Embryonic stem cells ; Embryos ; Fibroblasts ; Human Embryonic Stem Cells - metabolism ; Human Embryonic Stem Cells - physiology ; Humans ; Isoforms ; Laboratory animals ; Laminin ; Laminin - metabolism ; Medicin och hälsovetenskap ; Mice ; mRNA ; Pluripotency ; Protein Isoforms - metabolism ; Proteins ; Retinoic acid ; RNA, Messenger - metabolism ; Stem cells ; Transcription factors ; Tretinoin</subject><ispartof>PloS one, 2015-09, Vol.10 (9), p.e0138346-e0138346</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Pook et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Pook et al 2015 Pook et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c846t-230d892031d069524cab274e7228c9283533b1c41d74b09f49912054427b661d3</citedby><cites>FETCH-LOGICAL-c846t-230d892031d069524cab274e7228c9283533b1c41d74b09f49912054427b661d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574950/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574950/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26378917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:132070897$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Zambidis, Elias T.</contributor><creatorcontrib>Pook, Martin</creatorcontrib><creatorcontrib>Teino, Indrek</creatorcontrib><creatorcontrib>Kallas, Ade</creatorcontrib><creatorcontrib>Maimets, Toivo</creatorcontrib><creatorcontrib>Ingerpuu, Sulev</creatorcontrib><creatorcontrib>Jaks, Viljar</creatorcontrib><title>Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Laminin isoforms laminin-511 and -521 are expressed by human embryonic stem cells (hESC) and can be used as a growth matrix to culture these cells under pluripotent conditions. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pook, Martin</au><au>Teino, Indrek</au><au>Kallas, Ade</au><au>Maimets, Toivo</au><au>Ingerpuu, Sulev</au><au>Jaks, Viljar</au><au>Zambidis, Elias T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-09-17</date><risdate>2015</risdate><volume>10</volume><issue>9</issue><spage>e0138346</spage><epage>e0138346</epage><pages>e0138346-e0138346</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Laminin isoforms laminin-511 and -521 are expressed by human embryonic stem cells (hESC) and can be used as a growth matrix to culture these cells under pluripotent conditions. However, the expression of these laminins during the induction of hESC differentiation has not been studied in detail. Furthermore, the data regarding the expression pattern of laminin chains in differentiating hESC is scarce. In the current study we aimed to fill this gap and investigated the potential changes in laminin expression during early hESC differentiation induced by retinoic acid (RA). We found that laminin-511 but not -521 accumulates in the committed cells during early steps of hESC differentiation. We also performed a comprehensive analysis of the laminin chain repertoire and found that pluripotent hESC express a more diverse range of laminin chains than shown previously. In particular, we provide the evidence that in addition to α1, α5, β1, β2 and γ1 chains, hESC express α2, α3, β3, γ2 and γ3 chain proteins and mRNA. Additionally, we found that a variant of laminin α3 chain-145 kDa-accumulated in RA-treated hESC showing that these cells produce prevalently specifically modified version of α3 chain in early phase of differentiation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26378917</pmid><doi>10.1371/journal.pone.0138346</doi><tpages>e0138346</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Binding sites Biology Cell adhesion & migration Cell culture Cell Differentiation - physiology Cells, Cultured Chains Differentiation Embryo cells Embryonic stem cells Embryos Fibroblasts Human Embryonic Stem Cells - metabolism Human Embryonic Stem Cells - physiology Humans Isoforms Laboratory animals Laminin Laminin - metabolism Medicin och hälsovetenskap Mice mRNA Pluripotency Protein Isoforms - metabolism Proteins Retinoic acid RNA, Messenger - metabolism Stem cells Transcription factors Tretinoin |
| title | Changes in Laminin Expression Pattern during Early Differentiation of Human Embryonic Stem Cells |
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