Plastid Proteomic Analysis in Tomato Fruit Development
To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. Additionally, to discover more about the relationship between fruit color and plastid differ...
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| Veröffentlicht in: | PloS one 2015-09, Vol.10 (9), p.e0137266-e0137266 |
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| creator | Suzuki, Miho Takahashi, Sachiko Kondo, Takanori Dohra, Hideo Ito, Yumihiko Kiriiwa, Yoshikazu Hayashi, Marina Kamiya, Shiori Kato, Masaya Fujiwara, Masayuki Fukao, Yoichiro Kobayashi, Megumi Nagata, Noriko Motohashi, Reiko |
| description | To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. Additionally, to discover more about the relationship between fruit color and plastid differentiation, we also analyzed and compared 'Micro-Tom' results with those from two other varieties, 'Black' and 'White Beauty'. We confirmed that proteins related to photosynthesis remain through the orange maturity stage of 'Micro-Tom', and also learned that thylakoids no longer exist at this stage. These results suggest that at a minimum there are changes in plastid morphology occurring before all related proteins change. We also compared 'Micro-Tom' fruits with 'Black' and 'White Beauty' using two-dimensional gel electrophoresis. We found a decrease of CHRC (plastid-lipid-associated protein) and HrBP1 (harpin binding protein-1) in the 'Black' and 'White Beauty' varieties. CHRC is involved in carotenoid accumulation and stabilization. HrBP1 in Arabidopsis has a sequence similar to proteins in the PAP/fibrillin family. These proteins have characteristics and functions similar to lipocalin, an example of which is the transport of hydrophobic molecules. We detected spots of TIL (temperature-induced lipocalin) in 2D-PAGE results, however the number of spots and their isoelectric points differed between 'Micro-Tom' and 'Black'/'White Beauty'. Lipocalin has various functions including those related to environmental stress response, apoptosis induction, membrane formation and fixation, regulation of immune response, cell growth, and metabolism adjustment. Lipocalin related proteins such as TIL and HrBP1 could be related to the accumulation of carotenoids, fruit color and the differentiation of chromoplast. |
| doi_str_mv | 10.1371/journal.pone.0137266 |
| format | Article |
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Additionally, to discover more about the relationship between fruit color and plastid differentiation, we also analyzed and compared 'Micro-Tom' results with those from two other varieties, 'Black' and 'White Beauty'. We confirmed that proteins related to photosynthesis remain through the orange maturity stage of 'Micro-Tom', and also learned that thylakoids no longer exist at this stage. These results suggest that at a minimum there are changes in plastid morphology occurring before all related proteins change. We also compared 'Micro-Tom' fruits with 'Black' and 'White Beauty' using two-dimensional gel electrophoresis. We found a decrease of CHRC (plastid-lipid-associated protein) and HrBP1 (harpin binding protein-1) in the 'Black' and 'White Beauty' varieties. CHRC is involved in carotenoid accumulation and stabilization. HrBP1 in Arabidopsis has a sequence similar to proteins in the PAP/fibrillin family. These proteins have characteristics and functions similar to lipocalin, an example of which is the transport of hydrophobic molecules. We detected spots of TIL (temperature-induced lipocalin) in 2D-PAGE results, however the number of spots and their isoelectric points differed between 'Micro-Tom' and 'Black'/'White Beauty'. Lipocalin has various functions including those related to environmental stress response, apoptosis induction, membrane formation and fixation, regulation of immune response, cell growth, and metabolism adjustment. Lipocalin related proteins such as TIL and HrBP1 could be related to the accumulation of carotenoids, fruit color and the differentiation of chromoplast.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0137266</identifier><identifier>PMID: 26371478</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Apoptosis ; Arabidopsis thaliana ; Biology ; Biosynthesis ; Carotenoids ; Carotenoids - metabolism ; Cellular stress response ; Chloroplasts ; Chloroplasts - metabolism ; Citrus sinensis ; Color ; Developmental stages ; Differentiation ; Environmental stress ; Fibrillin ; Flavonoids ; Flavonoids - metabolism ; Fruit - growth & development ; Fruits ; Gel electrophoresis ; Genetic aspects ; Genomes ; Hydrophobicity ; Identification ; Immune response ; Immune system ; Isoelectric points ; Lipocalin ; Metabolism ; Photosynthesis ; Physiological aspects ; Pigmentation ; Plant development ; Plant Proteins - metabolism ; Plant sciences ; Plastids ; Plastids - metabolism ; Proteins ; Proteomics ; Rice ; Science education ; Solanum lycopersicum ; Solanum lycopersicum - cytology ; Solanum lycopersicum - genetics ; Solanum lycopersicum - growth & development ; Solanum lycopersicum - metabolism ; Studies ; Thylakoids ; Tomatoes</subject><ispartof>PloS one, 2015-09, Vol.10 (9), p.e0137266-e0137266</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Suzuki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Suzuki et al 2015 Suzuki et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c736t-4c00d9a949769b40cfbb207b25ecf015e677c67b10e25a27e40bac48adfde73</citedby><cites>FETCH-LOGICAL-c736t-4c00d9a949769b40cfbb207b25ecf015e677c67b10e25a27e40bac48adfde73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570674/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570674/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26371478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Miho</creatorcontrib><creatorcontrib>Takahashi, Sachiko</creatorcontrib><creatorcontrib>Kondo, Takanori</creatorcontrib><creatorcontrib>Dohra, Hideo</creatorcontrib><creatorcontrib>Ito, Yumihiko</creatorcontrib><creatorcontrib>Kiriiwa, Yoshikazu</creatorcontrib><creatorcontrib>Hayashi, Marina</creatorcontrib><creatorcontrib>Kamiya, Shiori</creatorcontrib><creatorcontrib>Kato, Masaya</creatorcontrib><creatorcontrib>Fujiwara, Masayuki</creatorcontrib><creatorcontrib>Fukao, Yoichiro</creatorcontrib><creatorcontrib>Kobayashi, Megumi</creatorcontrib><creatorcontrib>Nagata, Noriko</creatorcontrib><creatorcontrib>Motohashi, Reiko</creatorcontrib><title>Plastid Proteomic Analysis in Tomato Fruit Development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. 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These proteins have characteristics and functions similar to lipocalin, an example of which is the transport of hydrophobic molecules. We detected spots of TIL (temperature-induced lipocalin) in 2D-PAGE results, however the number of spots and their isoelectric points differed between 'Micro-Tom' and 'Black'/'White Beauty'. Lipocalin has various functions including those related to environmental stress response, apoptosis induction, membrane formation and fixation, regulation of immune response, cell growth, and metabolism adjustment. Lipocalin related proteins such as TIL and HrBP1 could be related to the accumulation of carotenoids, fruit color and the differentiation of chromoplast.</description><subject>Accumulation</subject><subject>Apoptosis</subject><subject>Arabidopsis thaliana</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Carotenoids</subject><subject>Carotenoids - metabolism</subject><subject>Cellular stress response</subject><subject>Chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>Citrus sinensis</subject><subject>Color</subject><subject>Developmental stages</subject><subject>Differentiation</subject><subject>Environmental stress</subject><subject>Fibrillin</subject><subject>Flavonoids</subject><subject>Flavonoids - metabolism</subject><subject>Fruit - growth & development</subject><subject>Fruits</subject><subject>Gel electrophoresis</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Hydrophobicity</subject><subject>Identification</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Isoelectric points</subject><subject>Lipocalin</subject><subject>Metabolism</subject><subject>Photosynthesis</subject><subject>Physiological aspects</subject><subject>Pigmentation</subject><subject>Plant development</subject><subject>Plant Proteins - metabolism</subject><subject>Plant sciences</subject><subject>Plastids</subject><subject>Plastids - metabolism</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Rice</subject><subject>Science education</subject><subject>Solanum lycopersicum</subject><subject>Solanum lycopersicum - cytology</subject><subject>Solanum lycopersicum - genetics</subject><subject>Solanum lycopersicum - growth & development</subject><subject>Solanum lycopersicum - metabolism</subject><subject>Studies</subject><subject>Thylakoids</subject><subject>Tomatoes</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbF39B6IDgujFrvmaZOZGWKrVhUKLLd6GTObMbkpmsk0yxf57s91p2ZFeSC4STp7z5pyTN8veYrTAVOAv127wvbKLrethgVKIcP4sO8YVJXNOEH1-cD7KXoVwjVBBS85fZkeEJwUmyuOMX1gVomnyC-8iuM7ofJlU74IJuenzK9ep6PJTP5iYf4NbsG7bQR9fZy9aZQO8GfdZdnn6_erk5_zs_MfqZHk214LyOGcaoaZSFasEr2qGdFvXBImaFKBbhAvgQmguaoyAFIoIYKhWmpWqaRsQdJa936turQtybDhILHBFSlIilojVnmicupZbbzrl76RTRt4HnF9L5aPRFmQaAy9bhSvgNWtpU6VR1Bgz1tYV00wnra_ja0PdQaNTm17Ziej0pjcbuXa3khUCcbEr5tMo4N3NACHKzgQN1qoe3HBfNy0ooamSWfbhH_Tp7kZqrVIDpm9delfvROWSkVIgVBY7rcUTVFoNpO9M7mhNik8SPk8SEhPhT1yrIQS5uvz1_-z57yn78YDdgLJxE5wdonF9mIJsD2rvQvDQPg4ZI7kz98M05M7ccjR3Snt3-EGPSQ9upn8Bz2Py2g</recordid><startdate>20150915</startdate><enddate>20150915</enddate><creator>Suzuki, Miho</creator><creator>Takahashi, Sachiko</creator><creator>Kondo, Takanori</creator><creator>Dohra, Hideo</creator><creator>Ito, Yumihiko</creator><creator>Kiriiwa, Yoshikazu</creator><creator>Hayashi, Marina</creator><creator>Kamiya, Shiori</creator><creator>Kato, Masaya</creator><creator>Fujiwara, Masayuki</creator><creator>Fukao, Yoichiro</creator><creator>Kobayashi, Megumi</creator><creator>Nagata, Noriko</creator><creator>Motohashi, Reiko</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150915</creationdate><title>Plastid Proteomic Analysis in Tomato Fruit Development</title><author>Suzuki, Miho ; Takahashi, Sachiko ; Kondo, Takanori ; Dohra, Hideo ; Ito, Yumihiko ; Kiriiwa, Yoshikazu ; Hayashi, Marina ; Kamiya, Shiori ; Kato, Masaya ; Fujiwara, Masayuki ; Fukao, Yoichiro ; Kobayashi, Megumi ; Nagata, Noriko ; Motohashi, Reiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c736t-4c00d9a949769b40cfbb207b25ecf015e677c67b10e25a27e40bac48adfde73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Accumulation</topic><topic>Apoptosis</topic><topic>Arabidopsis thaliana</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Carotenoids</topic><topic>Carotenoids - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Miho</au><au>Takahashi, Sachiko</au><au>Kondo, Takanori</au><au>Dohra, Hideo</au><au>Ito, Yumihiko</au><au>Kiriiwa, Yoshikazu</au><au>Hayashi, Marina</au><au>Kamiya, Shiori</au><au>Kato, Masaya</au><au>Fujiwara, Masayuki</au><au>Fukao, Yoichiro</au><au>Kobayashi, Megumi</au><au>Nagata, Noriko</au><au>Motohashi, Reiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plastid Proteomic Analysis in Tomato Fruit Development</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-09-15</date><risdate>2015</risdate><volume>10</volume><issue>9</issue><spage>e0137266</spage><epage>e0137266</epage><pages>e0137266-e0137266</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. Additionally, to discover more about the relationship between fruit color and plastid differentiation, we also analyzed and compared 'Micro-Tom' results with those from two other varieties, 'Black' and 'White Beauty'. We confirmed that proteins related to photosynthesis remain through the orange maturity stage of 'Micro-Tom', and also learned that thylakoids no longer exist at this stage. These results suggest that at a minimum there are changes in plastid morphology occurring before all related proteins change. We also compared 'Micro-Tom' fruits with 'Black' and 'White Beauty' using two-dimensional gel electrophoresis. We found a decrease of CHRC (plastid-lipid-associated protein) and HrBP1 (harpin binding protein-1) in the 'Black' and 'White Beauty' varieties. CHRC is involved in carotenoid accumulation and stabilization. HrBP1 in Arabidopsis has a sequence similar to proteins in the PAP/fibrillin family. These proteins have characteristics and functions similar to lipocalin, an example of which is the transport of hydrophobic molecules. We detected spots of TIL (temperature-induced lipocalin) in 2D-PAGE results, however the number of spots and their isoelectric points differed between 'Micro-Tom' and 'Black'/'White Beauty'. Lipocalin has various functions including those related to environmental stress response, apoptosis induction, membrane formation and fixation, regulation of immune response, cell growth, and metabolism adjustment. Lipocalin related proteins such as TIL and HrBP1 could be related to the accumulation of carotenoids, fruit color and the differentiation of chromoplast.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26371478</pmid><doi>10.1371/journal.pone.0137266</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-09, Vol.10 (9), p.e0137266-e0137266 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1719282804 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Accumulation Apoptosis Arabidopsis thaliana Biology Biosynthesis Carotenoids Carotenoids - metabolism Cellular stress response Chloroplasts Chloroplasts - metabolism Citrus sinensis Color Developmental stages Differentiation Environmental stress Fibrillin Flavonoids Flavonoids - metabolism Fruit - growth & development Fruits Gel electrophoresis Genetic aspects Genomes Hydrophobicity Identification Immune response Immune system Isoelectric points Lipocalin Metabolism Photosynthesis Physiological aspects Pigmentation Plant development Plant Proteins - metabolism Plant sciences Plastids Plastids - metabolism Proteins Proteomics Rice Science education Solanum lycopersicum Solanum lycopersicum - cytology Solanum lycopersicum - genetics Solanum lycopersicum - growth & development Solanum lycopersicum - metabolism Studies Thylakoids Tomatoes |
| title | Plastid Proteomic Analysis in Tomato Fruit Development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T18%3A00%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plastid%20Proteomic%20Analysis%20in%20Tomato%20Fruit%20Development&rft.jtitle=PloS%20one&rft.au=Suzuki,%20Miho&rft.date=2015-09-15&rft.volume=10&rft.issue=9&rft.spage=e0137266&rft.epage=e0137266&rft.pages=e0137266-e0137266&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0137266&rft_dat=%3Cgale_plos_%3EA428700850%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1719282804&rft_id=info:pmid/26371478&rft_galeid=A428700850&rft_doaj_id=oai_doaj_org_article_20368fa19e6b4f3d9866b1144fb94c4c&rfr_iscdi=true |