Deciphering Seed Sequence Based Off-Target Effects in a Large-Scale RNAi Reporter Screen for E-Cadherin Expression

Functional RNAi based screening is affected by large numbers of false positive and negative hits due to prevalent sequence based off-target effects. We performed a druggable genome targeting siRNA screen intended to identify novel regulators of E-cadherin (CDH1) expression, a known key player in epi...

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Veröffentlicht in:	PloS one 2015-09, Vol.10 (9), p.e0137640-e0137640
Hauptverfasser:	Adams, Robert, Nicke, Barbara, Pohlenz, Hans-Dieter, Sohler, Florian
Format:	Artikel
Sprache:	eng
Schlagworte:	Breast cancer Cadherins Cadherins - genetics Cadherins - metabolism Cell cycle Cell Line, Tumor E-cadherin Gene expression Genetic screening Genome, Human Genomes Genotype & phenotype Health aspects High-Throughput Screening Assays - methods Humans Mesenchyme Metastasis Methods Molecular targeted therapy Nucleotide sequence Pathogens Properties Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Regulators RNA Interference RNA, Small Interfering - genetics RNA-mediated interference Screening Sensitivity and Specificity Sensors siRNA Trans-Activators - genetics Trans-Activators - metabolism
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