Measuring Meiotic Crossovers via Multi-Locus Genotyping of Single Pollen Grains in Barley

The detection of meiotic crossovers in crop plants currently relies on scoring DNA markers in a segregating population or cytological visualization. We investigated the feasibility of using flow-sorted haploid nuclei, Phi29 DNA polymerase-based whole-genome-amplification (WGA) and multi-locus KASP-g...

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Veröffentlicht in:	PloS one 2015-09, Vol.10 (9), p.e0137677-e0137677
Hauptverfasser:	Dreissig, Steven, Fuchs, Jörg, Cápal, Petr, Kettles, Nicola, Byrne, Ed, Houben, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis Arabidopsis thaliana Barley Biological effects Cell cycle Chromosomes, Plant Crossing over (Genetics) Crossing Over, Genetic Crossovers Deoxyribonucleic acid Distortion DNA DNA methylation DNA polymerase DNA, Plant DNA-directed DNA polymerase Enzymes Feasibility studies Genetic aspects Genetics Genome, Plant Genomes Genotyping Grains Haploidy Homogenization Hordeum - genetics Hordeum vulgare Loci Meiosis Meiosis - genetics Multilocus Sequence Typing Mutation Nuclei Nuclei (cytology) Physiological aspects Plants (botany) Pollen Pollen - genetics Quantitative Trait Loci Recombination Recombination, Genetic Single-nucleotide polymorphism
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