Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis res...
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| creator | Xu, Jing Xing, Xiao-Juan Tian, Yong-Sheng Peng, Ri-He Xue, Yong Zhao, Wei Yao, Quan-Hong |
| description | Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis. |
| doi_str_mv | 10.1371/journal.pone.0136960 |
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In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0136960</identifier><identifier>PMID: 26327625</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic stress ; Adaptation, Physiological - genetics ; Agrobacterium tumefaciens ; Agrobacterium tumefaciens - genetics ; Aluminum ; Analysis ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis thaliana ; Biotechnology ; Cloning ; Drought ; Droughts ; Enzymes ; Flowers ; Gene expression ; Gene Expression Regulation, Plant - genetics ; Genes ; Genetic aspects ; Genetic transformation ; Glutathione ; Glutathione transferase ; Glutathione Transferase - genetics ; Heat stress ; Heat tolerance ; Kinases ; Lycopersicon esculentum ; Lycopersicon esculentum - genetics ; Malondialdehyde ; Mannitol ; Metabolism ; Methods ; Mosses ; Osmotic Pressure - physiology ; Osmotic stress ; Oxidative stress ; Physiological aspects ; Plant Proteins - genetics ; Plant Roots - genetics ; Plants, Genetically Modified - genetics ; Proline ; Rice ; Salinity ; Salt ; Salt-Tolerance - genetics ; Salts ; Seeds ; Sodium Chloride ; Stress management ; Stress, Physiological - genetics ; Tobacco ; Tomatoes ; Transcription ; Transformation ; Transgenic plants ; Trends</subject><ispartof>PloS one, 2015-09, Vol.10 (9), p.e0136960-e0136960</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Xu et al 2015 Xu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-cf18bfe374693958353ddd07c6bd9b9d6fb3621ba76db73285fc608fa20804103</citedby><cites>FETCH-LOGICAL-c692t-cf18bfe374693958353ddd07c6bd9b9d6fb3621ba76db73285fc608fa20804103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556630/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556630/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26327625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Hong</contributor><creatorcontrib>Xu, Jing</creatorcontrib><creatorcontrib>Xing, Xiao-Juan</creatorcontrib><creatorcontrib>Tian, Yong-Sheng</creatorcontrib><creatorcontrib>Peng, Ri-He</creatorcontrib><creatorcontrib>Xue, Yong</creatorcontrib><creatorcontrib>Zhao, Wei</creatorcontrib><creatorcontrib>Yao, Quan-Hong</creatorcontrib><title>Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.</description><subject>Abiotic stress</subject><subject>Adaptation, Physiological - genetics</subject><subject>Agrobacterium tumefaciens</subject><subject>Agrobacterium tumefaciens - genetics</subject><subject>Aluminum</subject><subject>Analysis</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Biotechnology</subject><subject>Cloning</subject><subject>Drought</subject><subject>Droughts</subject><subject>Enzymes</subject><subject>Flowers</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic transformation</subject><subject>Glutathione</subject><subject>Glutathione transferase</subject><subject>Glutathione Transferase - genetics</subject><subject>Heat stress</subject><subject>Heat tolerance</subject><subject>Kinases</subject><subject>Lycopersicon esculentum</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Malondialdehyde</subject><subject>Mannitol</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Mosses</subject><subject>Osmotic Pressure - physiology</subject><subject>Osmotic stress</subject><subject>Oxidative stress</subject><subject>Physiological aspects</subject><subject>Plant Proteins - genetics</subject><subject>Plant Roots - genetics</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Proline</subject><subject>Rice</subject><subject>Salinity</subject><subject>Salt</subject><subject>Salt-Tolerance - genetics</subject><subject>Salts</subject><subject>Seeds</subject><subject>Sodium Chloride</subject><subject>Stress management</subject><subject>Stress, Physiological - genetics</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Transcription</subject><subject>Transformation</subject><subject>Transgenic plants</subject><subject>Trends</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKPxE5ukKpRRqVJQ2vh1nJsJ_GU2sV2YFzx13HabGrQLlAucuI873vs43OS5CUEc4gp_HBje2d4N99Zo-YAYlIS8Cg5hSVGM4IAfnwUnyTPvL8BIMcFIU-TE0QwogTlp8mfjePGN8pokS4cr7S0O699-rXjJvh0ebtzynttmnRjtzzY9KLrAw-tjlnT9WyvrpXjPn619peS6dK03IgYXKtoFIY4jbo170LKjUw_Ods3bUjXYXB-njypeefVi_F9lnz7vNycf5ldXl2szheXM0FKFGaihkVVK0wzUuIyL3COpZSAClLJsiolqStMEKw4JbKiGBV5LQgoao5AATII8Fny-uC766xnY-08gxQUJcrzjEZidSCk5Tds5_SWu9_Mcs32C9Y1jLugRaeYwrioSlzQgsAMYliAvJYU4VwpSrEcvD6O2fpqq6RQJjjeTUynf4xuWWN_sizPCcHDdt-NBs7-6JUPbKu9UF28FWX7_b6HOmCMIvrmH_Th041Uw-MBtKltzCsGU7bIhl5AZQkjNX-Aio9UWy3ildc6rk8E7yeCyAR1Gxree89W6-v_Z6--T9m3R2yrYvO03sbWi33np2B2AIWz3jtV3xcZAjbMyV012DAnbJyTKHt1fEH3orvBwH8BrfgNsA</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Xu, Jing</creator><creator>Xing, Xiao-Juan</creator><creator>Tian, Yong-Sheng</creator><creator>Peng, Ri-He</creator><creator>Xue, Yong</creator><creator>Zhao, Wei</creator><creator>Yao, Quan-Hong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150901</creationdate><title>Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress</title><author>Xu, Jing ; Xing, Xiao-Juan ; Tian, Yong-Sheng ; Peng, Ri-He ; Xue, Yong ; Zhao, Wei ; Yao, Quan-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-cf18bfe374693958353ddd07c6bd9b9d6fb3621ba76db73285fc608fa20804103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abiotic stress</topic><topic>Adaptation, Physiological - 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In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26327625</pmid><doi>10.1371/journal.pone.0136960</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Abiotic stress Adaptation, Physiological - genetics Agrobacterium tumefaciens Agrobacterium tumefaciens - genetics Aluminum Analysis Arabidopsis Arabidopsis - genetics Arabidopsis thaliana Biotechnology Cloning Drought Droughts Enzymes Flowers Gene expression Gene Expression Regulation, Plant - genetics Genes Genetic aspects Genetic transformation Glutathione Glutathione transferase Glutathione Transferase - genetics Heat stress Heat tolerance Kinases Lycopersicon esculentum Lycopersicon esculentum - genetics Malondialdehyde Mannitol Metabolism Methods Mosses Osmotic Pressure - physiology Osmotic stress Oxidative stress Physiological aspects Plant Proteins - genetics Plant Roots - genetics Plants, Genetically Modified - genetics Proline Rice Salinity Salt Salt-Tolerance - genetics Salts Seeds Sodium Chloride Stress management Stress, Physiological - genetics Tobacco Tomatoes Transcription Transformation Transgenic plants Trends |
| title | Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress |
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