De Novo Transcriptome Analysis of Warburgia ugandensis to Identify Genes Involved in Terpenoids and Unsaturated Fatty Acids Biosynthesis
The bark of Warburgia ugandensis (Canellaceae family) has been used as a medicinal source for a long history in many African countries. The presence of diverse terpenoids and abundant polyunsaturated fatty acids (PUFAs) in this organ contributes to its broad range of pharmacological properties. Desp...
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| description | The bark of Warburgia ugandensis (Canellaceae family) has been used as a medicinal source for a long history in many African countries. The presence of diverse terpenoids and abundant polyunsaturated fatty acids (PUFAs) in this organ contributes to its broad range of pharmacological properties. Despite its medicinal and economic importance, the knowledge on the biosynthesis of terpenoid and unsaturated fatty acid in W. ugandensis bark remains largely unknown. Therefore, it is necessary to construct a genomic and/or transcriptomic database for the functional genomics study on W. ugandensis. The chemical profiles of terpenoids and fatty acids between the bark and leaves of W. ugandensis were compared by gas chromatography-mass spectrometry (GC-MS) analysis. Meanwhile, the transcriptome database derived from both tissues was created using Illumina sequencing technology. In total, about 17.1 G clean nucleotides were obtained, and de novo assembled into 72,591 unigenes, of which about 38.06% can be aligned to the NCBI non-redundant protein database. Many candidate genes in the biosynthetic pathways of terpenoids and unsaturated fatty acids were identified, including 14 unigenes for terpene synthases. Furthermore, 2,324 unigenes were discovered to be differentially expressed between both tissues; the functions of those differentially expressed genes (DEGs) were predicted by gene ontology enrichment and metabolic pathway enrichment analyses. In addition, the expression of 12 DEGs with putative roles in terpenoid and unsaturated fatty acid metabolic pathways was confirmed by qRT-PCRs, which was consistent with the data of the RNA-sequencing. In conclusion, we constructed a comprehensive transcriptome dataset derived from the bark and leaf of W. ugandensis, which forms the basis for functional genomics studies on this plant species. Particularly, the comparative analysis of the transcriptome data between the bark and leaf will provide critical clues to reveal the regulatory mechanisms underlying the biosynthesis of terpenoids and PUFAs in W. ugandensis. |
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The presence of diverse terpenoids and abundant polyunsaturated fatty acids (PUFAs) in this organ contributes to its broad range of pharmacological properties. Despite its medicinal and economic importance, the knowledge on the biosynthesis of terpenoid and unsaturated fatty acid in W. ugandensis bark remains largely unknown. Therefore, it is necessary to construct a genomic and/or transcriptomic database for the functional genomics study on W. ugandensis. The chemical profiles of terpenoids and fatty acids between the bark and leaves of W. ugandensis were compared by gas chromatography-mass spectrometry (GC-MS) analysis. Meanwhile, the transcriptome database derived from both tissues was created using Illumina sequencing technology. In total, about 17.1 G clean nucleotides were obtained, and de novo assembled into 72,591 unigenes, of which about 38.06% can be aligned to the NCBI non-redundant protein database. Many candidate genes in the biosynthetic pathways of terpenoids and unsaturated fatty acids were identified, including 14 unigenes for terpene synthases. Furthermore, 2,324 unigenes were discovered to be differentially expressed between both tissues; the functions of those differentially expressed genes (DEGs) were predicted by gene ontology enrichment and metabolic pathway enrichment analyses. In addition, the expression of 12 DEGs with putative roles in terpenoid and unsaturated fatty acid metabolic pathways was confirmed by qRT-PCRs, which was consistent with the data of the RNA-sequencing. In conclusion, we constructed a comprehensive transcriptome dataset derived from the bark and leaf of W. ugandensis, which forms the basis for functional genomics studies on this plant species. Particularly, the comparative analysis of the transcriptome data between the bark and leaf will provide critical clues to reveal the regulatory mechanisms underlying the biosynthesis of terpenoids and PUFAs in W. ugandensis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0135724</identifier><identifier>PMID: 26305373</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bark ; Biosynthesis ; Biosynthetic Pathways - genetics ; Cardiovascular disease ; Catalysis ; Comparative analysis ; Data bases ; Economic importance ; Enrichment ; Enzymes ; Fatty acids ; Fatty Acids, Unsaturated - biosynthesis ; Flowers & plants ; Gas chromatography ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Plant ; Gene Ontology ; Gene sequencing ; Genes ; Genes, Plant ; Genomes ; Genomics ; Germplasm ; Laboratories ; Leaves ; Lipid Metabolism - genetics ; Magnoliopsida - genetics ; Mammals ; Mass spectrometry ; Mass spectroscopy ; Metabolic pathways ; Molecular Sequence Annotation ; Nucleotides ; Pathways ; Pharmacology ; Phylogeny ; Phytochemicals ; Plant Bark - chemistry ; Plant Bark - genetics ; Plant Leaves - chemistry ; Plant Leaves - genetics ; Plant species ; Plant tissues ; Polyunsaturated fatty acids ; Proteins ; Real-Time Polymerase Chain Reaction ; Regulatory mechanisms (biology) ; Ribonucleic acid ; RNA ; Salvia miltiorrhiza ; Sequence Analysis, RNA ; Terpenes ; Terpenes - metabolism</subject><ispartof>PloS one, 2015-08, Vol.10 (8), p.e0135724-e0135724</ispartof><rights>2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Wang et al 2015 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-fafe1a6993c421dafeab8154837913599fde2b4b7de0d770efe2c49ff9d75f893</citedby><cites>FETCH-LOGICAL-c526t-fafe1a6993c421dafeab8154837913599fde2b4b7de0d770efe2c49ff9d75f893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549110/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4549110/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26305373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Li, Maoteng</contributor><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Zhou, Chen</creatorcontrib><creatorcontrib>Yang, Xianpeng</creatorcontrib><creatorcontrib>Miao, Di</creatorcontrib><creatorcontrib>Zhang, Yansheng</creatorcontrib><title>De Novo Transcriptome Analysis of Warburgia ugandensis to Identify Genes Involved in Terpenoids and Unsaturated Fatty Acids Biosynthesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The bark of Warburgia ugandensis (Canellaceae family) has been used as a medicinal source for a long history in many African countries. The presence of diverse terpenoids and abundant polyunsaturated fatty acids (PUFAs) in this organ contributes to its broad range of pharmacological properties. Despite its medicinal and economic importance, the knowledge on the biosynthesis of terpenoid and unsaturated fatty acid in W. ugandensis bark remains largely unknown. Therefore, it is necessary to construct a genomic and/or transcriptomic database for the functional genomics study on W. ugandensis. The chemical profiles of terpenoids and fatty acids between the bark and leaves of W. ugandensis were compared by gas chromatography-mass spectrometry (GC-MS) analysis. Meanwhile, the transcriptome database derived from both tissues was created using Illumina sequencing technology. In total, about 17.1 G clean nucleotides were obtained, and de novo assembled into 72,591 unigenes, of which about 38.06% can be aligned to the NCBI non-redundant protein database. Many candidate genes in the biosynthetic pathways of terpenoids and unsaturated fatty acids were identified, including 14 unigenes for terpene synthases. Furthermore, 2,324 unigenes were discovered to be differentially expressed between both tissues; the functions of those differentially expressed genes (DEGs) were predicted by gene ontology enrichment and metabolic pathway enrichment analyses. In addition, the expression of 12 DEGs with putative roles in terpenoid and unsaturated fatty acid metabolic pathways was confirmed by qRT-PCRs, which was consistent with the data of the RNA-sequencing. In conclusion, we constructed a comprehensive transcriptome dataset derived from the bark and leaf of W. ugandensis, which forms the basis for functional genomics studies on this plant species. Particularly, the comparative analysis of the transcriptome data between the bark and leaf will provide critical clues to reveal the regulatory mechanisms underlying the biosynthesis of terpenoids and PUFAs in W. ugandensis.</description><subject>Bark</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways - genetics</subject><subject>Cardiovascular disease</subject><subject>Catalysis</subject><subject>Comparative analysis</subject><subject>Data bases</subject><subject>Economic importance</subject><subject>Enrichment</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>Fatty Acids, Unsaturated - biosynthesis</subject><subject>Flowers & plants</subject><subject>Gas chromatography</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Ontology</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Germplasm</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Lipid Metabolism - genetics</subject><subject>Magnoliopsida - genetics</subject><subject>Mammals</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolic pathways</subject><subject>Molecular Sequence Annotation</subject><subject>Nucleotides</subject><subject>Pathways</subject><subject>Pharmacology</subject><subject>Phylogeny</subject><subject>Phytochemicals</subject><subject>Plant Bark - chemistry</subject><subject>Plant Bark - genetics</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - genetics</subject><subject>Plant species</subject><subject>Plant tissues</subject><subject>Polyunsaturated fatty acids</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regulatory mechanisms (biology)</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Salvia miltiorrhiza</subject><subject>Sequence Analysis, RNA</subject><subject>Terpenes</subject><subject>Terpenes - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUstuEzEUHSEQLYU_QGCJDZsEezy2xxukUGiJVMEmFUvL47lOHU3sYHsi5Q_4bJxHqxax8vW955z70KmqtwRPCRXk0yqM0ethugkepphQJurmWXVOJK0nvMb0-aP4rHqV0gpjRlvOX1ZnNaclFvS8-vMV0I-wDWgRtU8muk0Oa0CzorxLLqFg0S8duzEunUbjUvse_D6fA5qXMDu7Q9fgIaG534ZhCz1yHi0gbsAH1ydUGOjWJ53HqHOpXumcd2hm9rUvLqSdz3dQFF9XL6weErw5vRfV7dW3xeX3yc3P6_nl7GZiWM3zxGoLRHMpqWlq0pef7lrCmpYKWW4gpe2h7ppO9IB7ITBYqE0jrZW9YLaV9KJ6f9TdDCGp0xGTIgK3jLeY4IKYHxF90Cu1iW6t404F7dQhEeJS6ZidGUDJtmOdoBYa0zZdyzU2WNY1r4lpOBesaH0-dRu7NfSmXCzq4Yno04p3d2oZtqphjSSHYT6eBGL4PULKau2SgWHQHsJ4mFswxjhtC_TDP9D_b9ccUSaGlCLYh2EIVntj3bPU3ljqZKxCe_d4kQfSvZPoX3uZzq8</recordid><startdate>20150825</startdate><enddate>20150825</enddate><creator>Wang, Xin</creator><creator>Zhou, Chen</creator><creator>Yang, Xianpeng</creator><creator>Miao, Di</creator><creator>Zhang, Yansheng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150825</creationdate><title>De Novo Transcriptome Analysis of Warburgia ugandensis to Identify Genes Involved in Terpenoids and Unsaturated Fatty Acids Biosynthesis</title><author>Wang, Xin ; Zhou, Chen ; Yang, Xianpeng ; Miao, Di ; Zhang, Yansheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-fafe1a6993c421dafeab8154837913599fde2b4b7de0d770efe2c49ff9d75f893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bark</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways - genetics</topic><topic>Cardiovascular disease</topic><topic>Catalysis</topic><topic>Comparative analysis</topic><topic>Data bases</topic><topic>Economic importance</topic><topic>Enrichment</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>Fatty Acids, Unsaturated - biosynthesis</topic><topic>Flowers & plants</topic><topic>Gas chromatography</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Ontology</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Germplasm</topic><topic>Laboratories</topic><topic>Leaves</topic><topic>Lipid Metabolism - genetics</topic><topic>Magnoliopsida - genetics</topic><topic>Mammals</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolic pathways</topic><topic>Molecular Sequence Annotation</topic><topic>Nucleotides</topic><topic>Pathways</topic><topic>Pharmacology</topic><topic>Phylogeny</topic><topic>Phytochemicals</topic><topic>Plant Bark - chemistry</topic><topic>Plant Bark - genetics</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - genetics</topic><topic>Plant species</topic><topic>Plant tissues</topic><topic>Polyunsaturated fatty acids</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Regulatory mechanisms (biology)</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Salvia miltiorrhiza</topic><topic>Sequence Analysis, RNA</topic><topic>Terpenes</topic><topic>Terpenes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Zhou, Chen</creatorcontrib><creatorcontrib>Yang, Xianpeng</creatorcontrib><creatorcontrib>Miao, Di</creatorcontrib><creatorcontrib>Zhang, Yansheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xin</au><au>Zhou, Chen</au><au>Yang, Xianpeng</au><au>Miao, Di</au><au>Zhang, Yansheng</au><au>Li, Maoteng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>De Novo Transcriptome Analysis of Warburgia ugandensis to Identify Genes Involved in Terpenoids and Unsaturated Fatty Acids Biosynthesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-08-25</date><risdate>2015</risdate><volume>10</volume><issue>8</issue><spage>e0135724</spage><epage>e0135724</epage><pages>e0135724-e0135724</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The bark of Warburgia ugandensis (Canellaceae family) has been used as a medicinal source for a long history in many African countries. The presence of diverse terpenoids and abundant polyunsaturated fatty acids (PUFAs) in this organ contributes to its broad range of pharmacological properties. Despite its medicinal and economic importance, the knowledge on the biosynthesis of terpenoid and unsaturated fatty acid in W. ugandensis bark remains largely unknown. Therefore, it is necessary to construct a genomic and/or transcriptomic database for the functional genomics study on W. ugandensis. The chemical profiles of terpenoids and fatty acids between the bark and leaves of W. ugandensis were compared by gas chromatography-mass spectrometry (GC-MS) analysis. Meanwhile, the transcriptome database derived from both tissues was created using Illumina sequencing technology. In total, about 17.1 G clean nucleotides were obtained, and de novo assembled into 72,591 unigenes, of which about 38.06% can be aligned to the NCBI non-redundant protein database. Many candidate genes in the biosynthetic pathways of terpenoids and unsaturated fatty acids were identified, including 14 unigenes for terpene synthases. Furthermore, 2,324 unigenes were discovered to be differentially expressed between both tissues; the functions of those differentially expressed genes (DEGs) were predicted by gene ontology enrichment and metabolic pathway enrichment analyses. In addition, the expression of 12 DEGs with putative roles in terpenoid and unsaturated fatty acid metabolic pathways was confirmed by qRT-PCRs, which was consistent with the data of the RNA-sequencing. In conclusion, we constructed a comprehensive transcriptome dataset derived from the bark and leaf of W. ugandensis, which forms the basis for functional genomics studies on this plant species. Particularly, the comparative analysis of the transcriptome data between the bark and leaf will provide critical clues to reveal the regulatory mechanisms underlying the biosynthesis of terpenoids and PUFAs in W. ugandensis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26305373</pmid><doi>10.1371/journal.pone.0135724</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-08, Vol.10 (8), p.e0135724-e0135724 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Bark Biosynthesis Biosynthetic Pathways - genetics Cardiovascular disease Catalysis Comparative analysis Data bases Economic importance Enrichment Enzymes Fatty acids Fatty Acids, Unsaturated - biosynthesis Flowers & plants Gas chromatography Gene expression Gene Expression Profiling - methods Gene Expression Regulation, Plant Gene Ontology Gene sequencing Genes Genes, Plant Genomes Genomics Germplasm Laboratories Leaves Lipid Metabolism - genetics Magnoliopsida - genetics Mammals Mass spectrometry Mass spectroscopy Metabolic pathways Molecular Sequence Annotation Nucleotides Pathways Pharmacology Phylogeny Phytochemicals Plant Bark - chemistry Plant Bark - genetics Plant Leaves - chemistry Plant Leaves - genetics Plant species Plant tissues Polyunsaturated fatty acids Proteins Real-Time Polymerase Chain Reaction Regulatory mechanisms (biology) Ribonucleic acid RNA Salvia miltiorrhiza Sequence Analysis, RNA Terpenes Terpenes - metabolism |
| title | De Novo Transcriptome Analysis of Warburgia ugandensis to Identify Genes Involved in Terpenoids and Unsaturated Fatty Acids Biosynthesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T10%3A25%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=De%20Novo%20Transcriptome%20Analysis%20of%20Warburgia%20ugandensis%20to%20Identify%20Genes%20Involved%20in%20Terpenoids%20and%20Unsaturated%20Fatty%20Acids%20Biosynthesis&rft.jtitle=PloS%20one&rft.au=Wang,%20Xin&rft.date=2015-08-25&rft.volume=10&rft.issue=8&rft.spage=e0135724&rft.epage=e0135724&rft.pages=e0135724-e0135724&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0135724&rft_dat=%3Cproquest_plos_%3E1707555638%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1708568010&rft_id=info:pmid/26305373&rft_doaj_id=oai_doaj_org_article_98b5b73fe4c84b86a0c0922621c46675&rfr_iscdi=true |