Extensive Genomic Diversity among Bovine-Adapted Staphylococcus aureus: Evidence for a Genomic Rearrangement within CC97
Staphylococcus aureus is an important pathogen associated with both human and veterinary disease and is a common cause of bovine mastitis. Genomic heterogeneity exists between S. aureus strains and has been implicated in the adaptation of specific strains to colonise particular mammalian hosts. Know...
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| description | Staphylococcus aureus is an important pathogen associated with both human and veterinary disease and is a common cause of bovine mastitis. Genomic heterogeneity exists between S. aureus strains and has been implicated in the adaptation of specific strains to colonise particular mammalian hosts. Knowledge of the factors required for host specificity and virulence is important for understanding the pathogenesis and management of S. aureus mastitis. In this study, a panel of mastitis-associated S. aureus isolates (n = 126) was tested for resistance to antibiotics commonly used to treat mastitis. Over half of the isolates (52%) demonstrated resistance to penicillin and ampicillin but all were susceptible to the other antibiotics tested. S. aureus isolates were further examined for their clonal diversity by Multi-Locus Sequence Typing (MLST). In total, 18 different sequence types (STs) were identified and eBURST analysis demonstrated that the majority of isolates grouped into clonal complexes CC97, CC151 or sequence type (ST) 136. Analysis of the role of recombination events in determining S. aureus population structure determined that ST diversification through nucleotide substitutions were more likely to be due to recombination compared to point mutation, with regions of the genome possibly acting as recombination hotspots. DNA microarray analysis revealed a large number of differences amongst S. aureus STs in their variable genome content, including genes associated with capsule and biofilm formation and adhesion factors. Finally, evidence for a genomic arrangement was observed within isolates from CC97 with the ST71-like subgroup showing evidence of an IS431 insertion element having replaced approximately 30 kb of DNA including the ica operon and histidine biosynthesis genes, resulting in histidine auxotrophy. This genomic rearrangement may be responsible for the diversification of ST71 into an emerging bovine adapted subgroup. |
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Genomic heterogeneity exists between S. aureus strains and has been implicated in the adaptation of specific strains to colonise particular mammalian hosts. Knowledge of the factors required for host specificity and virulence is important for understanding the pathogenesis and management of S. aureus mastitis. In this study, a panel of mastitis-associated S. aureus isolates (n = 126) was tested for resistance to antibiotics commonly used to treat mastitis. Over half of the isolates (52%) demonstrated resistance to penicillin and ampicillin but all were susceptible to the other antibiotics tested. S. aureus isolates were further examined for their clonal diversity by Multi-Locus Sequence Typing (MLST). In total, 18 different sequence types (STs) were identified and eBURST analysis demonstrated that the majority of isolates grouped into clonal complexes CC97, CC151 or sequence type (ST) 136. Analysis of the role of recombination events in determining S. aureus population structure determined that ST diversification through nucleotide substitutions were more likely to be due to recombination compared to point mutation, with regions of the genome possibly acting as recombination hotspots. DNA microarray analysis revealed a large number of differences amongst S. aureus STs in their variable genome content, including genes associated with capsule and biofilm formation and adhesion factors. Finally, evidence for a genomic arrangement was observed within isolates from CC97 with the ST71-like subgroup showing evidence of an IS431 insertion element having replaced approximately 30 kb of DNA including the ica operon and histidine biosynthesis genes, resulting in histidine auxotrophy. This genomic rearrangement may be responsible for the diversification of ST71 into an emerging bovine adapted subgroup.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0134592</identifier><identifier>PMID: 26317849</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Ampicillin ; Ampicillin - pharmacology ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Auxotrophy ; Bacterial infections ; Biofilms ; Biosynthesis ; Cattle ; Cattle Diseases - genetics ; Cattle Diseases - microbiology ; Deoxyribonucleic acid ; DNA ; DNA microarrays ; Drug resistance ; Drug Resistance, Bacterial - drug effects ; Female ; Gene Rearrangement ; Genes ; Genomes ; Genomics ; Histidine ; Host Specificity ; Mastitis ; Mastitis, Bovine - genetics ; Mastitis, Bovine - microbiology ; Milk - microbiology ; Multilocus sequence typing ; Multilocus Sequence Typing - methods ; Mutation ; Pathogenesis ; Pathogens ; Penicillin ; Penicillins - pharmacology ; Point mutation ; Population structure ; Recombination ; Recombination hot spots ; Staphylococcal Infections - genetics ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Staphylococcus infections ; Subgroups ; Veterinary colleges ; Veterinary medicine ; Virulence</subject><ispartof>PloS one, 2015-08, Vol.10 (8), p.e0134592-e0134592</ispartof><rights>2015 Budd et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Budd et al 2015 Budd et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-84851e83da8320e226ee1a195c576c4d494870d4b0c1ac75eb2908e30e6b95823</citedby><cites>FETCH-LOGICAL-c526t-84851e83da8320e226ee1a195c576c4d494870d4b0c1ac75eb2908e30e6b95823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552844/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552844/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26317849$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>van Schaik, Willem</contributor><creatorcontrib>Budd, Kathleen E</creatorcontrib><creatorcontrib>McCoy, Finola</creatorcontrib><creatorcontrib>Monecke, Stefan</creatorcontrib><creatorcontrib>Cormican, Paul</creatorcontrib><creatorcontrib>Mitchell, Jennifer</creatorcontrib><creatorcontrib>Keane, Orla M</creatorcontrib><title>Extensive Genomic Diversity among Bovine-Adapted Staphylococcus aureus: Evidence for a Genomic Rearrangement within CC97</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Staphylococcus aureus is an important pathogen associated with both human and veterinary disease and is a common cause of bovine mastitis. Genomic heterogeneity exists between S. aureus strains and has been implicated in the adaptation of specific strains to colonise particular mammalian hosts. Knowledge of the factors required for host specificity and virulence is important for understanding the pathogenesis and management of S. aureus mastitis. In this study, a panel of mastitis-associated S. aureus isolates (n = 126) was tested for resistance to antibiotics commonly used to treat mastitis. Over half of the isolates (52%) demonstrated resistance to penicillin and ampicillin but all were susceptible to the other antibiotics tested. S. aureus isolates were further examined for their clonal diversity by Multi-Locus Sequence Typing (MLST). In total, 18 different sequence types (STs) were identified and eBURST analysis demonstrated that the majority of isolates grouped into clonal complexes CC97, CC151 or sequence type (ST) 136. 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Genomic heterogeneity exists between S. aureus strains and has been implicated in the adaptation of specific strains to colonise particular mammalian hosts. Knowledge of the factors required for host specificity and virulence is important for understanding the pathogenesis and management of S. aureus mastitis. In this study, a panel of mastitis-associated S. aureus isolates (n = 126) was tested for resistance to antibiotics commonly used to treat mastitis. Over half of the isolates (52%) demonstrated resistance to penicillin and ampicillin but all were susceptible to the other antibiotics tested. S. aureus isolates were further examined for their clonal diversity by Multi-Locus Sequence Typing (MLST). In total, 18 different sequence types (STs) were identified and eBURST analysis demonstrated that the majority of isolates grouped into clonal complexes CC97, CC151 or sequence type (ST) 136. Analysis of the role of recombination events in determining S. aureus population structure determined that ST diversification through nucleotide substitutions were more likely to be due to recombination compared to point mutation, with regions of the genome possibly acting as recombination hotspots. DNA microarray analysis revealed a large number of differences amongst S. aureus STs in their variable genome content, including genes associated with capsule and biofilm formation and adhesion factors. Finally, evidence for a genomic arrangement was observed within isolates from CC97 with the ST71-like subgroup showing evidence of an IS431 insertion element having replaced approximately 30 kb of DNA including the ica operon and histidine biosynthesis genes, resulting in histidine auxotrophy. This genomic rearrangement may be responsible for the diversification of ST71 into an emerging bovine adapted subgroup.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26317849</pmid><doi>10.1371/journal.pone.0134592</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Ampicillin Ampicillin - pharmacology Animals Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents Auxotrophy Bacterial infections Biofilms Biosynthesis Cattle Cattle Diseases - genetics Cattle Diseases - microbiology Deoxyribonucleic acid DNA DNA microarrays Drug resistance Drug Resistance, Bacterial - drug effects Female Gene Rearrangement Genes Genomes Genomics Histidine Host Specificity Mastitis Mastitis, Bovine - genetics Mastitis, Bovine - microbiology Milk - microbiology Multilocus sequence typing Multilocus Sequence Typing - methods Mutation Pathogenesis Pathogens Penicillin Penicillins - pharmacology Point mutation Population structure Recombination Recombination hot spots Staphylococcal Infections - genetics Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus infections Subgroups Veterinary colleges Veterinary medicine Virulence |
| title | Extensive Genomic Diversity among Bovine-Adapted Staphylococcus aureus: Evidence for a Genomic Rearrangement within CC97 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T05%3A57%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extensive%20Genomic%20Diversity%20among%20Bovine-Adapted%20Staphylococcus%20aureus:%20Evidence%20for%20a%20Genomic%20Rearrangement%20within%20CC97&rft.jtitle=PloS%20one&rft.au=Budd,%20Kathleen%20E&rft.date=2015-08-28&rft.volume=10&rft.issue=8&rft.spage=e0134592&rft.epage=e0134592&rft.pages=e0134592-e0134592&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0134592&rft_dat=%3Cproquest_plos_%3E3793735521%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1708566672&rft_id=info:pmid/26317849&rft_doaj_id=oai_doaj_org_article_7b842b5ce73048eb8f32cac767bde295&rfr_iscdi=true |