Improved Assay for Quantifying a Redox Form of Angiotensinogen as a Biomarker for Pre-Eclampsia: A Case-Control Study
Angiotensinogen exists in two distinct redox forms in plasma, the oxidized sulfhydryl-bridge form and the reduced, unbridged, free thiol form. The oxidized form of angiotensinogen compared to the free thiol form preferentially interacts with renin resulting in increased generation of angiotensin. Th...
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| creator | Rahgozar, Soheila Amirian, Tayebeh Qi, Miao Shahshahan, Zahra Entezar-E-Ghaem, Mansureh Ghasemi Tehrani, Hatav Miroliaei, Mehran Krilis, Steven A Giannakopoulos, Bill |
| description | Angiotensinogen exists in two distinct redox forms in plasma, the oxidized sulfhydryl-bridge form and the reduced, unbridged, free thiol form. The oxidized form of angiotensinogen compared to the free thiol form preferentially interacts with renin resulting in increased generation of angiotensin. The predictive potential of the ratio of free-thiol to oxidized angiotensinogen in the plasma for pre-eclampsia was first suggested by the Read group in ref 10. We propose an improved method for determining the ratio and validate the method in a larger cohort of pregnant women.
Plasma samples from 115 individuals with pre-eclampsia and from 55 healthy pregnant control subjects were collected sequentially over a 2 year period. Using two distinct enzyme-linked immunosorbent assays (ELISAs) the plasma levels of total and free thiol angiotensinogen were quantified. The oxidized angiotensinogen plasma level is derived by subtracting the level of free thiol, reduced angiotensinogen from the total angiotensinogen levels in the plasma.
The relative proportion of free thiol angiotensinogen, expressed as a percentage of that observed with an in-house standard, is significantly decreased in pre-eclamptic patients (70.85% ± 29.49%) (mean ± SD) as compared to healthy pregnant controls (92.98 ± 24.93%) (mean ± SD) p ≤ 0.0001. The levels of total angiotensinogen did not differ between the two groups.
Patients with pre-eclampsia had substantially lower levels of free thiol angiotensinogen compared to healthy pregnant controls, whilst maintaining similar total angiotensinogen levels in the plasma. Hence, elevated levels of plasma oxidized angiotensinogen may be a contributing factor to hypertension in the setting of pre-eclampsia. |
| doi_str_mv | 10.1371/journal.pone.0135905 |
| format | Article |
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Plasma samples from 115 individuals with pre-eclampsia and from 55 healthy pregnant control subjects were collected sequentially over a 2 year period. Using two distinct enzyme-linked immunosorbent assays (ELISAs) the plasma levels of total and free thiol angiotensinogen were quantified. The oxidized angiotensinogen plasma level is derived by subtracting the level of free thiol, reduced angiotensinogen from the total angiotensinogen levels in the plasma.
The relative proportion of free thiol angiotensinogen, expressed as a percentage of that observed with an in-house standard, is significantly decreased in pre-eclamptic patients (70.85% ± 29.49%) (mean ± SD) as compared to healthy pregnant controls (92.98 ± 24.93%) (mean ± SD) p ≤ 0.0001. The levels of total angiotensinogen did not differ between the two groups.
Patients with pre-eclampsia had substantially lower levels of free thiol angiotensinogen compared to healthy pregnant controls, whilst maintaining similar total angiotensinogen levels in the plasma. Hence, elevated levels of plasma oxidized angiotensinogen may be a contributing factor to hypertension in the setting of pre-eclampsia.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0135905</identifier><identifier>PMID: 26312482</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Angiotensin ; Angiotensinogen ; Angiotensinogen - blood ; Angiotensinogen - chemistry ; Angiotensins ; Biological Assay - methods ; Biology ; Biomarkers ; Biomarkers - blood ; Care and treatment ; Case-Control Studies ; Comparative analysis ; Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Female ; Free radicals ; Genetic aspects ; Gynecology ; Health risk assessment ; Homocysteine ; Hospitals ; Humans ; Hypertension ; Immunoassays ; Immunology ; Infectious diseases ; Obstetrics ; Oxidation-Reduction ; Oxidative Stress ; Patients ; Physiological aspects ; Placenta ; Plasma ; Plasma levels ; Pre-eclampsia ; Pre-Eclampsia - blood ; Pre-Eclampsia - diagnosis ; Preeclampsia ; Pregnancy ; Renin ; ROC Curve ; Sample size ; Sampling methods ; Science ; Womens health</subject><ispartof>PloS one, 2015-08, Vol.10 (8), p.e0135905-e0135905</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Rahgozar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Rahgozar et al 2015 Rahgozar et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-1c87bfc6fd1171b522d6f1381bf2e8ebfd08945e3063735e0ba51b20c8c214ff3</citedby><cites>FETCH-LOGICAL-c692t-1c87bfc6fd1171b522d6f1381bf2e8ebfd08945e3063735e0ba51b20c8c214ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552422/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552422/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26312482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Obukhov, Alexander G</contributor><creatorcontrib>Rahgozar, Soheila</creatorcontrib><creatorcontrib>Amirian, Tayebeh</creatorcontrib><creatorcontrib>Qi, Miao</creatorcontrib><creatorcontrib>Shahshahan, Zahra</creatorcontrib><creatorcontrib>Entezar-E-Ghaem, Mansureh</creatorcontrib><creatorcontrib>Ghasemi Tehrani, Hatav</creatorcontrib><creatorcontrib>Miroliaei, Mehran</creatorcontrib><creatorcontrib>Krilis, Steven A</creatorcontrib><creatorcontrib>Giannakopoulos, Bill</creatorcontrib><title>Improved Assay for Quantifying a Redox Form of Angiotensinogen as a Biomarker for Pre-Eclampsia: A Case-Control Study</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Angiotensinogen exists in two distinct redox forms in plasma, the oxidized sulfhydryl-bridge form and the reduced, unbridged, free thiol form. The oxidized form of angiotensinogen compared to the free thiol form preferentially interacts with renin resulting in increased generation of angiotensin. The predictive potential of the ratio of free-thiol to oxidized angiotensinogen in the plasma for pre-eclampsia was first suggested by the Read group in ref 10. We propose an improved method for determining the ratio and validate the method in a larger cohort of pregnant women.
Plasma samples from 115 individuals with pre-eclampsia and from 55 healthy pregnant control subjects were collected sequentially over a 2 year period. Using two distinct enzyme-linked immunosorbent assays (ELISAs) the plasma levels of total and free thiol angiotensinogen were quantified. The oxidized angiotensinogen plasma level is derived by subtracting the level of free thiol, reduced angiotensinogen from the total angiotensinogen levels in the plasma.
The relative proportion of free thiol angiotensinogen, expressed as a percentage of that observed with an in-house standard, is significantly decreased in pre-eclamptic patients (70.85% ± 29.49%) (mean ± SD) as compared to healthy pregnant controls (92.98 ± 24.93%) (mean ± SD) p ≤ 0.0001. The levels of total angiotensinogen did not differ between the two groups.
Patients with pre-eclampsia had substantially lower levels of free thiol angiotensinogen compared to healthy pregnant controls, whilst maintaining similar total angiotensinogen levels in the plasma. Hence, elevated levels of plasma oxidized angiotensinogen may be a contributing factor to hypertension in the setting of pre-eclampsia.</description><subject>Adult</subject><subject>Angiotensin</subject><subject>Angiotensinogen</subject><subject>Angiotensinogen - blood</subject><subject>Angiotensinogen - chemistry</subject><subject>Angiotensins</subject><subject>Biological Assay - methods</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>Comparative analysis</subject><subject>Diagnosis</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Free radicals</subject><subject>Genetic aspects</subject><subject>Gynecology</subject><subject>Health risk assessment</subject><subject>Homocysteine</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hypertension</subject><subject>Immunoassays</subject><subject>Immunology</subject><subject>Infectious diseases</subject><subject>Obstetrics</subject><subject>Oxidation-Reduction</subject><subject>Oxidative Stress</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Placenta</subject><subject>Plasma</subject><subject>Plasma levels</subject><subject>Pre-eclampsia</subject><subject>Pre-Eclampsia - blood</subject><subject>Pre-Eclampsia - diagnosis</subject><subject>Preeclampsia</subject><subject>Pregnancy</subject><subject>Renin</subject><subject>ROC Curve</subject><subject>Sample size</subject><subject>Sampling methods</subject><subject>Science</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk-Fv1CAYxhujcXP6HxglMTH6oSdQaKkfTM7LppcsmW7qV0Ip9DhbOKFddv-93K5brmYfDB8g9Pc89HnhTZKXCM5QVqAPazd4K9rZxlk1gyijJaSPkmNUZjjNMcweH6yPkmchrCGkGcvzp8kRzjOECcPHybDsNt5dqxrMQxBboJ0H3wdhe6O3xjZAgEtVuxtw5nwHnAZz2xjXKxuMdY2yQISIfDauE_638rfyb16lp7IV3SYY8RHMwUIElS6c7b1rwVU_1NvnyRMt2qBejPNJ8vPs9Mfia3p-8WW5mJ-nMi9xnyLJikrLXNcIFaiiGNe5RhlDlcaKqUrXkJWEqgzmWZFRBStBUYWhZBIjonV2krze-25aF_hYscBRAVlMTwsUieWeqJ1Y8403MciWO2H47YbzDRe-N7JVnOiqQEUFRYUIKWgh4jUUDOZVrQmEQkSvT-NpQ9WpWqqYWLQT0-kXa1a8cdecUIoJxtHg3Wjg3Z9BhZ53JkjVtsIqN-z_m5UsJzSib_5BH043Uo2IAYzVLp4rd6Z8TnBeUsRIGanZA1QcteqMjM9Lm7g_EbyfCCLTq5u-EUMIfHl1-f_sxa8p-_aAXSnR9qvg2qE3zoYpSPag9C4Er_R9kRHku-64qwbfdQcfuyPKXh1e0L3orh2yv8dUCVI</recordid><startdate>20150827</startdate><enddate>20150827</enddate><creator>Rahgozar, Soheila</creator><creator>Amirian, Tayebeh</creator><creator>Qi, Miao</creator><creator>Shahshahan, Zahra</creator><creator>Entezar-E-Ghaem, Mansureh</creator><creator>Ghasemi Tehrani, Hatav</creator><creator>Miroliaei, Mehran</creator><creator>Krilis, Steven A</creator><creator>Giannakopoulos, Bill</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150827</creationdate><title>Improved Assay for Quantifying a Redox Form of Angiotensinogen as a Biomarker for Pre-Eclampsia: A Case-Control Study</title><author>Rahgozar, Soheila ; Amirian, Tayebeh ; Qi, Miao ; Shahshahan, Zahra ; Entezar-E-Ghaem, Mansureh ; Ghasemi Tehrani, Hatav ; Miroliaei, Mehran ; Krilis, Steven A ; Giannakopoulos, Bill</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-1c87bfc6fd1171b522d6f1381bf2e8ebfd08945e3063735e0ba51b20c8c214ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Angiotensin</topic><topic>Angiotensinogen</topic><topic>Angiotensinogen - blood</topic><topic>Angiotensinogen - chemistry</topic><topic>Angiotensins</topic><topic>Biological Assay - methods</topic><topic>Biology</topic><topic>Biomarkers</topic><topic>Biomarkers - blood</topic><topic>Care and treatment</topic><topic>Case-Control Studies</topic><topic>Comparative analysis</topic><topic>Diagnosis</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Free radicals</topic><topic>Genetic aspects</topic><topic>Gynecology</topic><topic>Health risk assessment</topic><topic>Homocysteine</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Hypertension</topic><topic>Immunoassays</topic><topic>Immunology</topic><topic>Infectious diseases</topic><topic>Obstetrics</topic><topic>Oxidation-Reduction</topic><topic>Oxidative Stress</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Placenta</topic><topic>Plasma</topic><topic>Plasma levels</topic><topic>Pre-eclampsia</topic><topic>Pre-Eclampsia - blood</topic><topic>Pre-Eclampsia - diagnosis</topic><topic>Preeclampsia</topic><topic>Pregnancy</topic><topic>Renin</topic><topic>ROC Curve</topic><topic>Sample size</topic><topic>Sampling methods</topic><topic>Science</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahgozar, Soheila</creatorcontrib><creatorcontrib>Amirian, Tayebeh</creatorcontrib><creatorcontrib>Qi, Miao</creatorcontrib><creatorcontrib>Shahshahan, Zahra</creatorcontrib><creatorcontrib>Entezar-E-Ghaem, Mansureh</creatorcontrib><creatorcontrib>Ghasemi Tehrani, Hatav</creatorcontrib><creatorcontrib>Miroliaei, Mehran</creatorcontrib><creatorcontrib>Krilis, Steven A</creatorcontrib><creatorcontrib>Giannakopoulos, Bill</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahgozar, Soheila</au><au>Amirian, Tayebeh</au><au>Qi, Miao</au><au>Shahshahan, Zahra</au><au>Entezar-E-Ghaem, Mansureh</au><au>Ghasemi Tehrani, Hatav</au><au>Miroliaei, Mehran</au><au>Krilis, Steven A</au><au>Giannakopoulos, Bill</au><au>Obukhov, Alexander G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved Assay for Quantifying a Redox Form of Angiotensinogen as a Biomarker for Pre-Eclampsia: A Case-Control Study</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-08-27</date><risdate>2015</risdate><volume>10</volume><issue>8</issue><spage>e0135905</spage><epage>e0135905</epage><pages>e0135905-e0135905</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Angiotensinogen exists in two distinct redox forms in plasma, the oxidized sulfhydryl-bridge form and the reduced, unbridged, free thiol form. The oxidized form of angiotensinogen compared to the free thiol form preferentially interacts with renin resulting in increased generation of angiotensin. The predictive potential of the ratio of free-thiol to oxidized angiotensinogen in the plasma for pre-eclampsia was first suggested by the Read group in ref 10. We propose an improved method for determining the ratio and validate the method in a larger cohort of pregnant women.
Plasma samples from 115 individuals with pre-eclampsia and from 55 healthy pregnant control subjects were collected sequentially over a 2 year period. Using two distinct enzyme-linked immunosorbent assays (ELISAs) the plasma levels of total and free thiol angiotensinogen were quantified. The oxidized angiotensinogen plasma level is derived by subtracting the level of free thiol, reduced angiotensinogen from the total angiotensinogen levels in the plasma.
The relative proportion of free thiol angiotensinogen, expressed as a percentage of that observed with an in-house standard, is significantly decreased in pre-eclamptic patients (70.85% ± 29.49%) (mean ± SD) as compared to healthy pregnant controls (92.98 ± 24.93%) (mean ± SD) p ≤ 0.0001. The levels of total angiotensinogen did not differ between the two groups.
Patients with pre-eclampsia had substantially lower levels of free thiol angiotensinogen compared to healthy pregnant controls, whilst maintaining similar total angiotensinogen levels in the plasma. Hence, elevated levels of plasma oxidized angiotensinogen may be a contributing factor to hypertension in the setting of pre-eclampsia.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26312482</pmid><doi>10.1371/journal.pone.0135905</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Angiotensin Angiotensinogen Angiotensinogen - blood Angiotensinogen - chemistry Angiotensins Biological Assay - methods Biology Biomarkers Biomarkers - blood Care and treatment Case-Control Studies Comparative analysis Diagnosis Enzyme-Linked Immunosorbent Assay Female Free radicals Genetic aspects Gynecology Health risk assessment Homocysteine Hospitals Humans Hypertension Immunoassays Immunology Infectious diseases Obstetrics Oxidation-Reduction Oxidative Stress Patients Physiological aspects Placenta Plasma Plasma levels Pre-eclampsia Pre-Eclampsia - blood Pre-Eclampsia - diagnosis Preeclampsia Pregnancy Renin ROC Curve Sample size Sampling methods Science Womens health |
| title | Improved Assay for Quantifying a Redox Form of Angiotensinogen as a Biomarker for Pre-Eclampsia: A Case-Control Study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T20%3A45%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improved%20Assay%20for%20Quantifying%20a%20Redox%20Form%20of%20Angiotensinogen%20as%20a%20Biomarker%20for%20Pre-Eclampsia:%20A%20Case-Control%20Study&rft.jtitle=PloS%20one&rft.au=Rahgozar,%20Soheila&rft.date=2015-08-27&rft.volume=10&rft.issue=8&rft.spage=e0135905&rft.epage=e0135905&rft.pages=e0135905-e0135905&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0135905&rft_dat=%3Cgale_plos_%3EA426951849%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1708482571&rft_id=info:pmid/26312482&rft_galeid=A426951849&rft_doaj_id=oai_doaj_org_article_4fb717b0ab144757a1377806bdf400aa&rfr_iscdi=true |