Impact of Cardiopulmonary Bypass on Respiratory Mucociliary Function in an Experimental Porcine Model
The impact of cardiac surgery using cardiopulmonary bypass (CPB) on the respiratory mucociliary function is unknown. This study evaluated the effects of CPB and interruption of mechanical ventilation on the respiratory mucociliary system. Twenty-two pigs were randomly assigned to the control (n = 10...
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| description | The impact of cardiac surgery using cardiopulmonary bypass (CPB) on the respiratory mucociliary function is unknown. This study evaluated the effects of CPB and interruption of mechanical ventilation on the respiratory mucociliary system.
Twenty-two pigs were randomly assigned to the control (n = 10) or CPB group (n = 12). After the induction of anesthesia, a tracheostomy was performed, and tracheal tissue samples were excised (T0) from both groups. All animals underwent thoracotomy. In the CPB group, an aorto-bicaval CPB was installed and maintained for 90 minutes. During the CPB, mechanical ventilation was interrupted, and the tracheal tube was disconnected. A second tracheal tissue sample was obtained 180 minutes after the tracheostomy (T180). Mucus samples were collected from the trachea using a bronchoscope at T0, T90 and T180. Ciliary beat frequency (CBF) and in situ mucociliary transport (MCT) were studied in ex vivo tracheal epithelium. Mucus viscosity (MV) was assessed using a cone-plate viscometer. Qualitative tracheal histological analysis was performed at T180 tissue samples.
CBF decreased in the CPB group (13.1 ± 1.9 Hz vs. 11.1 ± 2.1 Hz, p < 0.05) but not in the control group (13.1 ± 1 Hz vs. 13 ± 2.9 Hz). At T90, viscosity was increased in the CPB group compared to the control (p < 0.05). No significant differences were observed in in situ MCT. Tracheal histology in the CPB group showed areas of ciliated epithelium loss, submucosal edema and infiltration of inflammatory cells.
CPB acutely contributed to alterations in tracheal mucocilliary function. |
| doi_str_mv | 10.1371/journal.pone.0135564 |
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Twenty-two pigs were randomly assigned to the control (n = 10) or CPB group (n = 12). After the induction of anesthesia, a tracheostomy was performed, and tracheal tissue samples were excised (T0) from both groups. All animals underwent thoracotomy. In the CPB group, an aorto-bicaval CPB was installed and maintained for 90 minutes. During the CPB, mechanical ventilation was interrupted, and the tracheal tube was disconnected. A second tracheal tissue sample was obtained 180 minutes after the tracheostomy (T180). Mucus samples were collected from the trachea using a bronchoscope at T0, T90 and T180. Ciliary beat frequency (CBF) and in situ mucociliary transport (MCT) were studied in ex vivo tracheal epithelium. Mucus viscosity (MV) was assessed using a cone-plate viscometer. Qualitative tracheal histological analysis was performed at T180 tissue samples.
CBF decreased in the CPB group (13.1 ± 1.9 Hz vs. 11.1 ± 2.1 Hz, p < 0.05) but not in the control group (13.1 ± 1 Hz vs. 13 ± 2.9 Hz). At T90, viscosity was increased in the CPB group compared to the control (p < 0.05). No significant differences were observed in in situ MCT. Tracheal histology in the CPB group showed areas of ciliated epithelium loss, submucosal edema and infiltration of inflammatory cells.
CPB acutely contributed to alterations in tracheal mucocilliary function.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0135564</identifier><identifier>PMID: 26288020</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Anesthesia ; Anesthesiology ; Animals ; Cardiopulmonary Bypass - adverse effects ; Catheters ; Cilia beat frequency ; Coronary artery bypass ; Coronary vessels ; Diagnostic equipment (Medical) ; Edema ; Epithelium ; Esophagus ; Experiments ; Female ; Heart diseases ; Heart surgery ; Hemodynamics - physiology ; Histology ; Infiltration ; Inflammation ; Laboratory animals ; Male ; Mechanical ventilation ; Medical instruments ; Medicine ; Mucociliary Clearance - physiology ; Mucus ; Mucus - physiology ; Neutrophils ; Ostomy ; Outdoor air quality ; Pulmonary arteries ; Qualitative analysis ; Respiratory System ; Surgery ; Swine ; Thoracic Surgical Procedures - adverse effects ; Trachea ; Tracheostomy ; Veins & arteries ; Ventilation ; Ventilators ; Viscosity</subject><ispartof>PloS one, 2015-08, Vol.10 (8), p.e0135564-e0135564</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Sánchez-Véliz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Sánchez-Véliz et al 2015 Sánchez-Véliz et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-5e0836e4940d4e672d9990883f65384faeec74a68357ea5fcb4b37d601e9df133</citedby><cites>FETCH-LOGICAL-c692t-5e0836e4940d4e672d9990883f65384faeec74a68357ea5fcb4b37d601e9df133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545835/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545835/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26288020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Staffieri, Francesco</contributor><creatorcontrib>Sánchez-Véliz, Rodrigo</creatorcontrib><creatorcontrib>Carmona, Maria José</creatorcontrib><creatorcontrib>Otsuki, Denise Aya</creatorcontrib><creatorcontrib>Freitas, Claudia</creatorcontrib><creatorcontrib>Benício, Anderson</creatorcontrib><creatorcontrib>Negri, Elnara Marcia</creatorcontrib><creatorcontrib>Malbouisson, Luiz Marcelo</creatorcontrib><title>Impact of Cardiopulmonary Bypass on Respiratory Mucociliary Function in an Experimental Porcine Model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The impact of cardiac surgery using cardiopulmonary bypass (CPB) on the respiratory mucociliary function is unknown. This study evaluated the effects of CPB and interruption of mechanical ventilation on the respiratory mucociliary system.
Twenty-two pigs were randomly assigned to the control (n = 10) or CPB group (n = 12). After the induction of anesthesia, a tracheostomy was performed, and tracheal tissue samples were excised (T0) from both groups. All animals underwent thoracotomy. In the CPB group, an aorto-bicaval CPB was installed and maintained for 90 minutes. During the CPB, mechanical ventilation was interrupted, and the tracheal tube was disconnected. A second tracheal tissue sample was obtained 180 minutes after the tracheostomy (T180). Mucus samples were collected from the trachea using a bronchoscope at T0, T90 and T180. Ciliary beat frequency (CBF) and in situ mucociliary transport (MCT) were studied in ex vivo tracheal epithelium. Mucus viscosity (MV) was assessed using a cone-plate viscometer. Qualitative tracheal histological analysis was performed at T180 tissue samples.
CBF decreased in the CPB group (13.1 ± 1.9 Hz vs. 11.1 ± 2.1 Hz, p < 0.05) but not in the control group (13.1 ± 1 Hz vs. 13 ± 2.9 Hz). At T90, viscosity was increased in the CPB group compared to the control (p < 0.05). No significant differences were observed in in situ MCT. Tracheal histology in the CPB group showed areas of ciliated epithelium loss, submucosal edema and infiltration of inflammatory cells.
CPB acutely contributed to alterations in tracheal mucocilliary function.</description><subject>Analysis</subject><subject>Anesthesia</subject><subject>Anesthesiology</subject><subject>Animals</subject><subject>Cardiopulmonary Bypass - adverse effects</subject><subject>Catheters</subject><subject>Cilia beat frequency</subject><subject>Coronary artery bypass</subject><subject>Coronary vessels</subject><subject>Diagnostic equipment (Medical)</subject><subject>Edema</subject><subject>Epithelium</subject><subject>Esophagus</subject><subject>Experiments</subject><subject>Female</subject><subject>Heart diseases</subject><subject>Heart surgery</subject><subject>Hemodynamics - physiology</subject><subject>Histology</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Mechanical ventilation</subject><subject>Medical instruments</subject><subject>Medicine</subject><subject>Mucociliary Clearance - physiology</subject><subject>Mucus</subject><subject>Mucus - physiology</subject><subject>Neutrophils</subject><subject>Ostomy</subject><subject>Outdoor air quality</subject><subject>Pulmonary arteries</subject><subject>Qualitative analysis</subject><subject>Respiratory System</subject><subject>Surgery</subject><subject>Swine</subject><subject>Thoracic Surgical Procedures - adverse effects</subject><subject>Trachea</subject><subject>Tracheostomy</subject><subject>Veins & arteries</subject><subject>Ventilation</subject><subject>Ventilators</subject><subject>Viscosity</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk0tv1DAQxyMEoqXwDRBEQkJw2MXvJBeksmphpVZF5XG1vM5k15XXTu0Etd8ep5tWG9QD8iHW5Df_eXgmy15jNMe0wJ-ufB-csvPWO5gjTDkX7El2iCtKZoIg-nTvfpC9iPEKIU5LIZ5nB0SQskQEHWaw3LZKd7lv8oUKtfFtb7feqXCbf7ltVYy5d_klxNYE1flkPe-118aagTjtne5MAozLlctPbloIZguuUzb_7oM2DvJzX4N9mT1rlI3wavweZb9OT34uvs3OLr4uF8dnMy0q0s04oJIKYBVDNQNRkLqqKlSWtBEpc9YoAF0wJUrKC1C80Su2okUtEIaqbjClR9nbnW5rfZRjh6LEBSpZSZjAiVjuiNqrK9mmdFMh0isj7ww-rKUKndEWJGZNhShZJW3GgHNFoSiw5smkKBckaX0eo_WrLdQ6FR6UnYhO_zizkWv_RzLOeKohCXwYBYK_7iF2cmuiBmuVA9_f5c0LUuGqSui7f9DHqxuptUoFGNf4FFcPovKYpUcnJSuGsPNHqHRq2BqdxqkxyT5x-DhxSEwHN91a9THK5Y_L_2cvfk_Z93vsBpTtNtHbfpipOAXZDtTBxxigeWgyRnLYhvtuyGEb5LgNye3N_gM9ON2PP_0LONEEbg</recordid><startdate>20150819</startdate><enddate>20150819</enddate><creator>Sánchez-Véliz, Rodrigo</creator><creator>Carmona, Maria José</creator><creator>Otsuki, Denise Aya</creator><creator>Freitas, Claudia</creator><creator>Benício, Anderson</creator><creator>Negri, Elnara Marcia</creator><creator>Malbouisson, Luiz Marcelo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150819</creationdate><title>Impact of Cardiopulmonary Bypass on Respiratory Mucociliary Function in an Experimental Porcine Model</title><author>Sánchez-Véliz, Rodrigo ; Carmona, Maria José ; Otsuki, Denise Aya ; Freitas, Claudia ; Benício, Anderson ; Negri, Elnara Marcia ; Malbouisson, Luiz Marcelo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-5e0836e4940d4e672d9990883f65384faeec74a68357ea5fcb4b37d601e9df133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Anesthesia</topic><topic>Anesthesiology</topic><topic>Animals</topic><topic>Cardiopulmonary Bypass - adverse effects</topic><topic>Catheters</topic><topic>Cilia beat frequency</topic><topic>Coronary artery bypass</topic><topic>Coronary vessels</topic><topic>Diagnostic equipment (Medical)</topic><topic>Edema</topic><topic>Epithelium</topic><topic>Esophagus</topic><topic>Experiments</topic><topic>Female</topic><topic>Heart diseases</topic><topic>Heart surgery</topic><topic>Hemodynamics - physiology</topic><topic>Histology</topic><topic>Infiltration</topic><topic>Inflammation</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Mechanical ventilation</topic><topic>Medical instruments</topic><topic>Medicine</topic><topic>Mucociliary Clearance - physiology</topic><topic>Mucus</topic><topic>Mucus - physiology</topic><topic>Neutrophils</topic><topic>Ostomy</topic><topic>Outdoor air quality</topic><topic>Pulmonary arteries</topic><topic>Qualitative analysis</topic><topic>Respiratory System</topic><topic>Surgery</topic><topic>Swine</topic><topic>Thoracic Surgical Procedures - adverse effects</topic><topic>Trachea</topic><topic>Tracheostomy</topic><topic>Veins & arteries</topic><topic>Ventilation</topic><topic>Ventilators</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sánchez-Véliz, Rodrigo</creatorcontrib><creatorcontrib>Carmona, Maria José</creatorcontrib><creatorcontrib>Otsuki, Denise Aya</creatorcontrib><creatorcontrib>Freitas, Claudia</creatorcontrib><creatorcontrib>Benício, Anderson</creatorcontrib><creatorcontrib>Negri, Elnara Marcia</creatorcontrib><creatorcontrib>Malbouisson, Luiz Marcelo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sánchez-Véliz, Rodrigo</au><au>Carmona, Maria José</au><au>Otsuki, Denise Aya</au><au>Freitas, Claudia</au><au>Benício, Anderson</au><au>Negri, Elnara Marcia</au><au>Malbouisson, Luiz Marcelo</au><au>Staffieri, Francesco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of Cardiopulmonary Bypass on Respiratory Mucociliary Function in an Experimental Porcine Model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-08-19</date><risdate>2015</risdate><volume>10</volume><issue>8</issue><spage>e0135564</spage><epage>e0135564</epage><pages>e0135564-e0135564</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The impact of cardiac surgery using cardiopulmonary bypass (CPB) on the respiratory mucociliary function is unknown. This study evaluated the effects of CPB and interruption of mechanical ventilation on the respiratory mucociliary system.
Twenty-two pigs were randomly assigned to the control (n = 10) or CPB group (n = 12). After the induction of anesthesia, a tracheostomy was performed, and tracheal tissue samples were excised (T0) from both groups. All animals underwent thoracotomy. In the CPB group, an aorto-bicaval CPB was installed and maintained for 90 minutes. During the CPB, mechanical ventilation was interrupted, and the tracheal tube was disconnected. A second tracheal tissue sample was obtained 180 minutes after the tracheostomy (T180). Mucus samples were collected from the trachea using a bronchoscope at T0, T90 and T180. Ciliary beat frequency (CBF) and in situ mucociliary transport (MCT) were studied in ex vivo tracheal epithelium. Mucus viscosity (MV) was assessed using a cone-plate viscometer. Qualitative tracheal histological analysis was performed at T180 tissue samples.
CBF decreased in the CPB group (13.1 ± 1.9 Hz vs. 11.1 ± 2.1 Hz, p < 0.05) but not in the control group (13.1 ± 1 Hz vs. 13 ± 2.9 Hz). At T90, viscosity was increased in the CPB group compared to the control (p < 0.05). No significant differences were observed in in situ MCT. Tracheal histology in the CPB group showed areas of ciliated epithelium loss, submucosal edema and infiltration of inflammatory cells.
CPB acutely contributed to alterations in tracheal mucocilliary function.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26288020</pmid><doi>10.1371/journal.pone.0135564</doi><tpages>e0135564</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2015-08, Vol.10 (8), p.e0135564-e0135564 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1708482461 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Anesthesia Anesthesiology Animals Cardiopulmonary Bypass - adverse effects Catheters Cilia beat frequency Coronary artery bypass Coronary vessels Diagnostic equipment (Medical) Edema Epithelium Esophagus Experiments Female Heart diseases Heart surgery Hemodynamics - physiology Histology Infiltration Inflammation Laboratory animals Male Mechanical ventilation Medical instruments Medicine Mucociliary Clearance - physiology Mucus Mucus - physiology Neutrophils Ostomy Outdoor air quality Pulmonary arteries Qualitative analysis Respiratory System Surgery Swine Thoracic Surgical Procedures - adverse effects Trachea Tracheostomy Veins & arteries Ventilation Ventilators Viscosity |
| title | Impact of Cardiopulmonary Bypass on Respiratory Mucociliary Function in an Experimental Porcine Model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-01T07%3A43%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impact%20of%20Cardiopulmonary%20Bypass%20on%20Respiratory%20Mucociliary%20Function%20in%20an%20Experimental%20Porcine%20Model&rft.jtitle=PloS%20one&rft.au=S%C3%A1nchez-V%C3%A9liz,%20Rodrigo&rft.date=2015-08-19&rft.volume=10&rft.issue=8&rft.spage=e0135564&rft.epage=e0135564&rft.pages=e0135564-e0135564&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0135564&rft_dat=%3Cgale_plos_%3EA426228475%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1708482461&rft_id=info:pmid/26288020&rft_galeid=A426228475&rft_doaj_id=oai_doaj_org_article_14f9032be9d44e55a3e771c532ba3562&rfr_iscdi=true |