Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target h...
Gespeichert in:
Veröffentlicht in: | PloS one 2015-07, Vol.10 (7), p.e0134098 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 7 |
container_start_page | e0134098 |
container_title | PloS one |
container_volume | 10 |
creator | Rompikuntal, Pramod K Vdovikova, Svitlana Duperthuy, Marylise Johnson, Tanya L Åhlund, Monika Lundmark, Richard Oscarsson, Jan Sandkvist, Maria Uhlin, Bernt Eric Wai, Sun Nyunt |
description | Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV.
In this study, we demonstrated that PrtV was secreted from the wild type V. cholerae strain C6706 via the type II secretion system in association with OMVs. By immunoblotting and electron microscopic analysis using immunogold labeling, the association of PrtV with OMVs was examined. We demonstrated that OMV-associated PrtV was biologically active by showing altered morphology and detachment of cells when the human ileocecum carcinoma (HCT8) cells were treated with OMVs from the wild type V. cholerae strain C6706 whereas cells treated with OMVs from the prtV isogenic mutant showed no morphological changes. Furthermore, OMV-associated PrtV protease showed a contribution to bacterial resistance towards the antimicrobial peptide LL-37.
Our findings suggest that OMVs released from V. cholerae can deliver a processed, biologically active form of PrtV that contributes to bacterial interactions with target host cells. |
doi_str_mv | 10.1371/journal.pone.0134098 |
format | Article |
fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1700107523</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A455367888</galeid><doaj_id>oai_doaj_org_article_8be1738c04924c41af2b346d5fb59320</doaj_id><sourcerecordid>A455367888</sourcerecordid><originalsourceid>FETCH-LOGICAL-c730t-394f80a86e20448d66588f23ecf5b24c41fcc1e177012aa06d294c4bbccccbf63</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBEQkLiIsUfiePeTKrGgEqbOvGRW8txjltPSVxsB8a_x22zqZFAIrlIcvKc95zzyidJXmI0w7TE72_t4HrZzra2hxnCNEdz_ig5xXNKMkYQfXz0fpI88_4WoYJyxp4mJ4QRQlBenibVagjg0mvoaid7SCvwRrWQXUNjZIAmvbzbWhdSq9MbZxV4H2M3LlS7zwDSQ6qd7dLK1M7YVG1sC07C8-SJlq2HF-PzLPn-8fLbxefsavVpebG4ylRJUcjoPNccSc4gdpPzhrGCc00oKF3UJFc51kphwGWJMJESsYbMY7SuVbxqzehZ8vqgu22tF6MlXuASIYzKgtBILA9EY-Wt2DrTSfdbWGnEPmDdWkgXdjMLXsdKlCuUz_e1pSY1zVlT6LqITqKolR20_C_YDvVE7YOpFnu1oRtErM0Zj_z52N1Qd9Ao6IOT7SRt-qc3G7G2P0Ve4NhCEQXejALO_hjAh3-MOFJrGacwvbZRTHXGK7HIi4KykvNdM7O_UPFuoDMqHiJtYnyS8G6SEJkAd2EtB-_F8uuX_2dX1ZR9e8RuQLZh4207BGN7PwXzA6ic9d6BfnAOI7HbgXs3xG4HxLgDMe3VsesPSfeHnv4BuRkBNg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1700107523</pqid></control><display><type>article</type><title>Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>SWEPUB Freely available online</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Rompikuntal, Pramod K ; Vdovikova, Svitlana ; Duperthuy, Marylise ; Johnson, Tanya L ; Åhlund, Monika ; Lundmark, Richard ; Oscarsson, Jan ; Sandkvist, Maria ; Uhlin, Bernt Eric ; Wai, Sun Nyunt</creator><contributor>Freitag, Nancy E</contributor><creatorcontrib>Rompikuntal, Pramod K ; Vdovikova, Svitlana ; Duperthuy, Marylise ; Johnson, Tanya L ; Åhlund, Monika ; Lundmark, Richard ; Oscarsson, Jan ; Sandkvist, Maria ; Uhlin, Bernt Eric ; Wai, Sun Nyunt ; Freitag, Nancy E</creatorcontrib><description>Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV.
In this study, we demonstrated that PrtV was secreted from the wild type V. cholerae strain C6706 via the type II secretion system in association with OMVs. By immunoblotting and electron microscopic analysis using immunogold labeling, the association of PrtV with OMVs was examined. We demonstrated that OMV-associated PrtV was biologically active by showing altered morphology and detachment of cells when the human ileocecum carcinoma (HCT8) cells were treated with OMVs from the wild type V. cholerae strain C6706 whereas cells treated with OMVs from the prtV isogenic mutant showed no morphological changes. Furthermore, OMV-associated PrtV protease showed a contribution to bacterial resistance towards the antimicrobial peptide LL-37.
Our findings suggest that OMVs released from V. cholerae can deliver a processed, biologically active form of PrtV that contributes to bacterial interactions with target host cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0134098</identifier><identifier>PMID: 26222047</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Antimicrobial Cationic Peptides - pharmacology ; Bacteria ; Binding sites ; Biochemistry ; Biological activity ; Biophysics ; Cell Line, Tumor ; Cell Membrane - metabolism ; Cholera ; Cholera toxin ; Detachment ; Drug Resistance, Bacterial ; E coli ; Escherichia coli ; Extracellular Space - metabolism ; Genetic aspects ; Gram-negative bacteria ; Health aspects ; Humans ; Immunoblotting ; Immunology ; Infections ; Laboratories ; Medicine ; Membrane vesicles ; Microscopic analysis ; Molecular biology ; Pathogens ; Peptide Hydrolases - metabolism ; Physiological aspects ; Protease ; Proteases ; Protein Transport ; Proteinase ; Proteins ; Proteolysis ; Studies ; Toxins ; Vibrio cholerae ; Vibrio cholerae - cytology ; Vibrio cholerae - drug effects ; Vibrio cholerae - enzymology ; Vibrio cholerae - metabolism ; Water-borne diseases ; Waterborne diseases</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0134098</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Rompikuntal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Rompikuntal et al 2015 Rompikuntal et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c730t-394f80a86e20448d66588f23ecf5b24c41fcc1e177012aa06d294c4bbccccbf63</citedby><cites>FETCH-LOGICAL-c730t-394f80a86e20448d66588f23ecf5b24c41fcc1e177012aa06d294c4bbccccbf63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519245/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519245/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26222047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-107868$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Freitag, Nancy E</contributor><creatorcontrib>Rompikuntal, Pramod K</creatorcontrib><creatorcontrib>Vdovikova, Svitlana</creatorcontrib><creatorcontrib>Duperthuy, Marylise</creatorcontrib><creatorcontrib>Johnson, Tanya L</creatorcontrib><creatorcontrib>Åhlund, Monika</creatorcontrib><creatorcontrib>Lundmark, Richard</creatorcontrib><creatorcontrib>Oscarsson, Jan</creatorcontrib><creatorcontrib>Sandkvist, Maria</creatorcontrib><creatorcontrib>Uhlin, Bernt Eric</creatorcontrib><creatorcontrib>Wai, Sun Nyunt</creatorcontrib><title>Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV.
In this study, we demonstrated that PrtV was secreted from the wild type V. cholerae strain C6706 via the type II secretion system in association with OMVs. By immunoblotting and electron microscopic analysis using immunogold labeling, the association of PrtV with OMVs was examined. We demonstrated that OMV-associated PrtV was biologically active by showing altered morphology and detachment of cells when the human ileocecum carcinoma (HCT8) cells were treated with OMVs from the wild type V. cholerae strain C6706 whereas cells treated with OMVs from the prtV isogenic mutant showed no morphological changes. Furthermore, OMV-associated PrtV protease showed a contribution to bacterial resistance towards the antimicrobial peptide LL-37.
Our findings suggest that OMVs released from V. cholerae can deliver a processed, biologically active form of PrtV that contributes to bacterial interactions with target host cells.</description><subject>Analysis</subject><subject>Antimicrobial Cationic Peptides - pharmacology</subject><subject>Bacteria</subject><subject>Binding sites</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biophysics</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - metabolism</subject><subject>Cholera</subject><subject>Cholera toxin</subject><subject>Detachment</subject><subject>Drug Resistance, Bacterial</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Extracellular Space - metabolism</subject><subject>Genetic aspects</subject><subject>Gram-negative bacteria</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunology</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Medicine</subject><subject>Membrane vesicles</subject><subject>Microscopic analysis</subject><subject>Molecular biology</subject><subject>Pathogens</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Physiological aspects</subject><subject>Protease</subject><subject>Proteases</subject><subject>Protein Transport</subject><subject>Proteinase</subject><subject>Proteins</subject><subject>Proteolysis</subject><subject>Studies</subject><subject>Toxins</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - cytology</subject><subject>Vibrio cholerae - drug effects</subject><subject>Vibrio cholerae - enzymology</subject><subject>Vibrio cholerae - metabolism</subject><subject>Water-borne diseases</subject><subject>Waterborne diseases</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>D8T</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkLiIsUfiePeTKrGgEqbOvGRW8txjltPSVxsB8a_x22zqZFAIrlIcvKc95zzyidJXmI0w7TE72_t4HrZzra2hxnCNEdz_ig5xXNKMkYQfXz0fpI88_4WoYJyxp4mJ4QRQlBenibVagjg0mvoaid7SCvwRrWQXUNjZIAmvbzbWhdSq9MbZxV4H2M3LlS7zwDSQ6qd7dLK1M7YVG1sC07C8-SJlq2HF-PzLPn-8fLbxefsavVpebG4ylRJUcjoPNccSc4gdpPzhrGCc00oKF3UJFc51kphwGWJMJESsYbMY7SuVbxqzehZ8vqgu22tF6MlXuASIYzKgtBILA9EY-Wt2DrTSfdbWGnEPmDdWkgXdjMLXsdKlCuUz_e1pSY1zVlT6LqITqKolR20_C_YDvVE7YOpFnu1oRtErM0Zj_z52N1Qd9Ao6IOT7SRt-qc3G7G2P0Ve4NhCEQXejALO_hjAh3-MOFJrGacwvbZRTHXGK7HIi4KykvNdM7O_UPFuoDMqHiJtYnyS8G6SEJkAd2EtB-_F8uuX_2dX1ZR9e8RuQLZh4207BGN7PwXzA6ic9d6BfnAOI7HbgXs3xG4HxLgDMe3VsesPSfeHnv4BuRkBNg</recordid><startdate>20150729</startdate><enddate>20150729</enddate><creator>Rompikuntal, Pramod K</creator><creator>Vdovikova, Svitlana</creator><creator>Duperthuy, Marylise</creator><creator>Johnson, Tanya L</creator><creator>Åhlund, Monika</creator><creator>Lundmark, Richard</creator><creator>Oscarsson, Jan</creator><creator>Sandkvist, Maria</creator><creator>Uhlin, Bernt Eric</creator><creator>Wai, Sun Nyunt</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>ADHXS</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>D93</scope><scope>ZZAVC</scope><scope>DOA</scope></search><sort><creationdate>20150729</creationdate><title>Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae</title><author>Rompikuntal, Pramod K ; Vdovikova, Svitlana ; Duperthuy, Marylise ; Johnson, Tanya L ; Åhlund, Monika ; Lundmark, Richard ; Oscarsson, Jan ; Sandkvist, Maria ; Uhlin, Bernt Eric ; Wai, Sun Nyunt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c730t-394f80a86e20448d66588f23ecf5b24c41fcc1e177012aa06d294c4bbccccbf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Antimicrobial Cationic Peptides - pharmacology</topic><topic>Bacteria</topic><topic>Binding sites</topic><topic>Biochemistry</topic><topic>Biological activity</topic><topic>Biophysics</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - metabolism</topic><topic>Cholera</topic><topic>Cholera toxin</topic><topic>Detachment</topic><topic>Drug Resistance, Bacterial</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Extracellular Space - metabolism</topic><topic>Genetic aspects</topic><topic>Gram-negative bacteria</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunology</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Medicine</topic><topic>Membrane vesicles</topic><topic>Microscopic analysis</topic><topic>Molecular biology</topic><topic>Pathogens</topic><topic>Peptide Hydrolases - metabolism</topic><topic>Physiological aspects</topic><topic>Protease</topic><topic>Proteases</topic><topic>Protein Transport</topic><topic>Proteinase</topic><topic>Proteins</topic><topic>Proteolysis</topic><topic>Studies</topic><topic>Toxins</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - cytology</topic><topic>Vibrio cholerae - drug effects</topic><topic>Vibrio cholerae - enzymology</topic><topic>Vibrio cholerae - metabolism</topic><topic>Water-borne diseases</topic><topic>Waterborne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rompikuntal, Pramod K</creatorcontrib><creatorcontrib>Vdovikova, Svitlana</creatorcontrib><creatorcontrib>Duperthuy, Marylise</creatorcontrib><creatorcontrib>Johnson, Tanya L</creatorcontrib><creatorcontrib>Åhlund, Monika</creatorcontrib><creatorcontrib>Lundmark, Richard</creatorcontrib><creatorcontrib>Oscarsson, Jan</creatorcontrib><creatorcontrib>Sandkvist, Maria</creatorcontrib><creatorcontrib>Uhlin, Bernt Eric</creatorcontrib><creatorcontrib>Wai, Sun Nyunt</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SWEPUB Umeå universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Umeå universitet</collection><collection>SwePub Articles full text</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rompikuntal, Pramod K</au><au>Vdovikova, Svitlana</au><au>Duperthuy, Marylise</au><au>Johnson, Tanya L</au><au>Åhlund, Monika</au><au>Lundmark, Richard</au><au>Oscarsson, Jan</au><au>Sandkvist, Maria</au><au>Uhlin, Bernt Eric</au><au>Wai, Sun Nyunt</au><au>Freitag, Nancy E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-29</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0134098</spage><pages>e0134098-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV.
In this study, we demonstrated that PrtV was secreted from the wild type V. cholerae strain C6706 via the type II secretion system in association with OMVs. By immunoblotting and electron microscopic analysis using immunogold labeling, the association of PrtV with OMVs was examined. We demonstrated that OMV-associated PrtV was biologically active by showing altered morphology and detachment of cells when the human ileocecum carcinoma (HCT8) cells were treated with OMVs from the wild type V. cholerae strain C6706 whereas cells treated with OMVs from the prtV isogenic mutant showed no morphological changes. Furthermore, OMV-associated PrtV protease showed a contribution to bacterial resistance towards the antimicrobial peptide LL-37.
Our findings suggest that OMVs released from V. cholerae can deliver a processed, biologically active form of PrtV that contributes to bacterial interactions with target host cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26222047</pmid><doi>10.1371/journal.pone.0134098</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2015-07, Vol.10 (7), p.e0134098 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_1700107523 |
source | MEDLINE; DOAJ Directory of Open Access Journals; SWEPUB Freely available online; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Analysis Antimicrobial Cationic Peptides - pharmacology Bacteria Binding sites Biochemistry Biological activity Biophysics Cell Line, Tumor Cell Membrane - metabolism Cholera Cholera toxin Detachment Drug Resistance, Bacterial E coli Escherichia coli Extracellular Space - metabolism Genetic aspects Gram-negative bacteria Health aspects Humans Immunoblotting Immunology Infections Laboratories Medicine Membrane vesicles Microscopic analysis Molecular biology Pathogens Peptide Hydrolases - metabolism Physiological aspects Protease Proteases Protein Transport Proteinase Proteins Proteolysis Studies Toxins Vibrio cholerae Vibrio cholerae - cytology Vibrio cholerae - drug effects Vibrio cholerae - enzymology Vibrio cholerae - metabolism Water-borne diseases Waterborne diseases |
title | Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T03%3A58%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Outer%20Membrane%20Vesicle-Mediated%20Export%20of%20Processed%20PrtV%20Protease%20from%20Vibrio%20cholerae&rft.jtitle=PloS%20one&rft.au=Rompikuntal,%20Pramod%20K&rft.date=2015-07-29&rft.volume=10&rft.issue=7&rft.spage=e0134098&rft.pages=e0134098-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0134098&rft_dat=%3Cgale_plos_%3EA455367888%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1700107523&rft_id=info:pmid/26222047&rft_galeid=A455367888&rft_doaj_id=oai_doaj_org_article_8be1738c04924c41af2b346d5fb59320&rfr_iscdi=true |