Maternal Smoking History Enhances the Expression of Placental Growth Factor in Invasive Trophoblasts at Early Gestation Despite Cessation of Smoking
Maternal smoking during early pregnancy is associated with a reduced risk for preeclampsia even after smoking cessation during pregnancy. Although the pathophysiology of preeclampsia has not been established, placental growth factor (PlGF) is believed to be a key factor. The aim of this study was to...
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| creator | Kawashima, Akihiro Koide, Keiko Hasegawa, Junichi Arakaki, Tatsuya Takenaka, Shin Maruyama, Daisuke Matsuoka, Ryu Sekizawa, Akihiko |
| description | Maternal smoking during early pregnancy is associated with a reduced risk for preeclampsia even after smoking cessation during pregnancy. Although the pathophysiology of preeclampsia has not been established, placental growth factor (PlGF) is believed to be a key factor. The aim of this study was to assess the effect of maternal smoking on the PlGF expression in invasive trophoblasts at early gestation. We collected villous tissues from women requesting surgical termination due to non-medical reasons at 7-8 weeks of gestation. The maternal smoking status was evaluated by measuring the serum cotinine level and patients were divided into two groups: active smokers and non-smokers. After separating invasive trophoblasts from villous tissues cultured initially under 2% O2 for 24 hours, the separated invasive trophoblasts were cultured under 2% or 8% O2 for 48 hours. The expression levels of the PlGF gene in villous tissue specimens and in invasive trophoblasts cultured after the conditions were quantified using qRT-PCR. The levels of PlGF protein in the medium were quantified using an ELISA. The gene expression level of PlGF in the villi in the active-smokers was significantly higher than that of the non-smokers. In comparison of the invasive trophoblasts under normoxia and oxygenated conditions, the ratio of PlGF gene expression and protein expression under oxygenation (2% O2+8% O2 / 2% O2+2% O2) in the active-smokers were both significantly higher than in the non-smokers. Maternal smoking history appears to stimulate PlGF expression in invasive trophoblasts under oxygenated conditions. This may be one of several causes leading to the protective effect of maternal smoking on preeclampsia. |
| doi_str_mv | 10.1371/journal.pone.0134181 |
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Although the pathophysiology of preeclampsia has not been established, placental growth factor (PlGF) is believed to be a key factor. The aim of this study was to assess the effect of maternal smoking on the PlGF expression in invasive trophoblasts at early gestation. We collected villous tissues from women requesting surgical termination due to non-medical reasons at 7-8 weeks of gestation. The maternal smoking status was evaluated by measuring the serum cotinine level and patients were divided into two groups: active smokers and non-smokers. After separating invasive trophoblasts from villous tissues cultured initially under 2% O2 for 24 hours, the separated invasive trophoblasts were cultured under 2% or 8% O2 for 48 hours. The expression levels of the PlGF gene in villous tissue specimens and in invasive trophoblasts cultured after the conditions were quantified using qRT-PCR. The levels of PlGF protein in the medium were quantified using an ELISA. The gene expression level of PlGF in the villi in the active-smokers was significantly higher than that of the non-smokers. In comparison of the invasive trophoblasts under normoxia and oxygenated conditions, the ratio of PlGF gene expression and protein expression under oxygenation (2% O2+8% O2 / 2% O2+2% O2) in the active-smokers were both significantly higher than in the non-smokers. Maternal smoking history appears to stimulate PlGF expression in invasive trophoblasts under oxygenated conditions. This may be one of several causes leading to the protective effect of maternal smoking on preeclampsia.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0134181</identifier><identifier>PMID: 26214510</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Analysis ; Cotinine ; DNA methylation ; Drug addiction ; Enzyme-linked immunosorbent assay ; Epigenetics ; Female ; Gene expression ; Gene Expression Regulation ; Gestation ; Health risk assessment ; Humans ; Oxygen - metabolism ; Oxygen Consumption ; Oxygenation ; Placenta ; Placenta Growth Factor ; Pre-eclampsia ; Pre-Eclampsia - etiology ; Pre-Eclampsia - metabolism ; Pre-Eclampsia - pathology ; Preeclampsia ; Pregnancy ; Pregnancy Proteins - biosynthesis ; Pregnant women ; Prenatal development ; Smoking ; Smoking - adverse effects ; Smoking - metabolism ; Smoking Cessation ; Surgery ; Tissues ; Trophoblasts ; Trophoblasts - metabolism ; Trophoblasts - pathology</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0134181-e0134181</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Kawashima et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Kawashima et al 2015 Kawashima et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-c0946f1bff7fdb5a3c8cb1b6c2e1e980f0256db7d0f6e3c0cc26a94c33a7bddd3</citedby><cites>FETCH-LOGICAL-c758t-c0946f1bff7fdb5a3c8cb1b6c2e1e980f0256db7d0f6e3c0cc26a94c33a7bddd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516258/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516258/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26214510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Crispi-Brillas, Fatima</contributor><creatorcontrib>Kawashima, Akihiro</creatorcontrib><creatorcontrib>Koide, Keiko</creatorcontrib><creatorcontrib>Hasegawa, Junichi</creatorcontrib><creatorcontrib>Arakaki, Tatsuya</creatorcontrib><creatorcontrib>Takenaka, Shin</creatorcontrib><creatorcontrib>Maruyama, Daisuke</creatorcontrib><creatorcontrib>Matsuoka, Ryu</creatorcontrib><creatorcontrib>Sekizawa, Akihiko</creatorcontrib><title>Maternal Smoking History Enhances the Expression of Placental Growth Factor in Invasive Trophoblasts at Early Gestation Despite Cessation of Smoking</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Maternal smoking during early pregnancy is associated with a reduced risk for preeclampsia even after smoking cessation during pregnancy. Although the pathophysiology of preeclampsia has not been established, placental growth factor (PlGF) is believed to be a key factor. The aim of this study was to assess the effect of maternal smoking on the PlGF expression in invasive trophoblasts at early gestation. We collected villous tissues from women requesting surgical termination due to non-medical reasons at 7-8 weeks of gestation. The maternal smoking status was evaluated by measuring the serum cotinine level and patients were divided into two groups: active smokers and non-smokers. After separating invasive trophoblasts from villous tissues cultured initially under 2% O2 for 24 hours, the separated invasive trophoblasts were cultured under 2% or 8% O2 for 48 hours. The expression levels of the PlGF gene in villous tissue specimens and in invasive trophoblasts cultured after the conditions were quantified using qRT-PCR. The levels of PlGF protein in the medium were quantified using an ELISA. The gene expression level of PlGF in the villi in the active-smokers was significantly higher than that of the non-smokers. In comparison of the invasive trophoblasts under normoxia and oxygenated conditions, the ratio of PlGF gene expression and protein expression under oxygenation (2% O2+8% O2 / 2% O2+2% O2) in the active-smokers were both significantly higher than in the non-smokers. Maternal smoking history appears to stimulate PlGF expression in invasive trophoblasts under oxygenated conditions. 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adverse effects</subject><subject>Smoking - metabolism</subject><subject>Smoking Cessation</subject><subject>Surgery</subject><subject>Tissues</subject><subject>Trophoblasts</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - pathology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggsISG4aPGfxElukKbSdZWGhtjg1nIcu_Fw42I7ZX0PHhiHZlODdoEsxdbJ7_t8fOyTJC8RnCKSow83tnMtN9ONbeUUIpKiAj1KjlFJ8IRiSB4frI-SZ97fQJiRgtKnyRGmGKUZgsfJ7888yN4HXK3tD92uwLn2wbodmLcNb4X0IDQSzG83TnqvbQusAl8MF7INUbRw9ldowBkXUQN0C5btlnu9leDa2U1jK8N98IAHMOfO7MBC-sBDb_NJ-o0OEsyi7T4SjYccnidPFDdevhjmk-Tb2fx6dj65uFwsZ6cXE5FnRZgIWKZUoUqpXNVVxokoRIUqKrBEsiyggjijdZXXUFFJBBQCU16mghCeV3Vdk5Pk9d53Y6xnQ0E9Q7QsMUQoyyOx3BO15Tds4_Saux2zXLO_AetWjLughZFMpZRUZYG4KuMS4ZLEr1QVqWqcFmkVvT4Ou3XVWtZ9BR03I9Pxn1Y3bGW3LN4UxVkRDd4NBs7-7GIl2Vp7IY3hrbRdzDuHEMEM533eb_5BHz7dQK14PIBulY37it6UnaaYEFjSNI3U9AEqjlqutYivT-kYHwnejwSRCfI2rHjnPVteff1_9vL7mH17wDaSm9B4a7r-9fgxmO5B4az3Tqr7IiPI-ua5qwbrm4cNzRNlrw4v6F501y3kD3NeF0Q</recordid><startdate>20150727</startdate><enddate>20150727</enddate><creator>Kawashima, Akihiro</creator><creator>Koide, Keiko</creator><creator>Hasegawa, Junichi</creator><creator>Arakaki, Tatsuya</creator><creator>Takenaka, Shin</creator><creator>Maruyama, Daisuke</creator><creator>Matsuoka, Ryu</creator><creator>Sekizawa, Akihiko</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150727</creationdate><title>Maternal Smoking History Enhances the Expression of Placental Growth Factor in Invasive Trophoblasts at Early Gestation Despite Cessation of Smoking</title><author>Kawashima, Akihiro ; Koide, Keiko ; Hasegawa, Junichi ; Arakaki, Tatsuya ; Takenaka, Shin ; Maruyama, Daisuke ; Matsuoka, Ryu ; Sekizawa, Akihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-c0946f1bff7fdb5a3c8cb1b6c2e1e980f0256db7d0f6e3c0cc26a94c33a7bddd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Cotinine</topic><topic>DNA methylation</topic><topic>Drug addiction</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epigenetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gestation</topic><topic>Health risk assessment</topic><topic>Humans</topic><topic>Oxygen - 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Although the pathophysiology of preeclampsia has not been established, placental growth factor (PlGF) is believed to be a key factor. The aim of this study was to assess the effect of maternal smoking on the PlGF expression in invasive trophoblasts at early gestation. We collected villous tissues from women requesting surgical termination due to non-medical reasons at 7-8 weeks of gestation. The maternal smoking status was evaluated by measuring the serum cotinine level and patients were divided into two groups: active smokers and non-smokers. After separating invasive trophoblasts from villous tissues cultured initially under 2% O2 for 24 hours, the separated invasive trophoblasts were cultured under 2% or 8% O2 for 48 hours. The expression levels of the PlGF gene in villous tissue specimens and in invasive trophoblasts cultured after the conditions were quantified using qRT-PCR. The levels of PlGF protein in the medium were quantified using an ELISA. The gene expression level of PlGF in the villi in the active-smokers was significantly higher than that of the non-smokers. In comparison of the invasive trophoblasts under normoxia and oxygenated conditions, the ratio of PlGF gene expression and protein expression under oxygenation (2% O2+8% O2 / 2% O2+2% O2) in the active-smokers were both significantly higher than in the non-smokers. Maternal smoking history appears to stimulate PlGF expression in invasive trophoblasts under oxygenated conditions. This may be one of several causes leading to the protective effect of maternal smoking on preeclampsia.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26214510</pmid><doi>10.1371/journal.pone.0134181</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-07, Vol.10 (7), p.e0134181-e0134181 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Analysis Cotinine DNA methylation Drug addiction Enzyme-linked immunosorbent assay Epigenetics Female Gene expression Gene Expression Regulation Gestation Health risk assessment Humans Oxygen - metabolism Oxygen Consumption Oxygenation Placenta Placenta Growth Factor Pre-eclampsia Pre-Eclampsia - etiology Pre-Eclampsia - metabolism Pre-Eclampsia - pathology Preeclampsia Pregnancy Pregnancy Proteins - biosynthesis Pregnant women Prenatal development Smoking Smoking - adverse effects Smoking - metabolism Smoking Cessation Surgery Tissues Trophoblasts Trophoblasts - metabolism Trophoblasts - pathology |
| title | Maternal Smoking History Enhances the Expression of Placental Growth Factor in Invasive Trophoblasts at Early Gestation Despite Cessation of Smoking |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T14%3A29%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Maternal%20Smoking%20History%20Enhances%20the%20Expression%20of%20Placental%20Growth%20Factor%20in%20Invasive%20Trophoblasts%20at%20Early%20Gestation%20Despite%20Cessation%20of%20Smoking&rft.jtitle=PloS%20one&rft.au=Kawashima,%20Akihiro&rft.date=2015-07-27&rft.volume=10&rft.issue=7&rft.spage=e0134181&rft.epage=e0134181&rft.pages=e0134181-e0134181&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0134181&rft_dat=%3Cgale_plos_%3EA423309644%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1699201157&rft_id=info:pmid/26214510&rft_galeid=A423309644&rft_doaj_id=oai_doaj_org_article_f463b981af9f461293461efb3bd2484b&rfr_iscdi=true |