Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles
Environmental DNA (eDNA) is a potentially powerful tool for detection and monitoring of rare species, including threatened native species and recently arrived invasive species. Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can b...
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| Veröffentlicht in: | PloS one 2015-07, Vol.10 (7), p.e0130965-e0130965 |
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| creator | Davy, Christina M Kidd, Anne G Wilson, Chris C |
| description | Environmental DNA (eDNA) is a potentially powerful tool for detection and monitoring of rare species, including threatened native species and recently arrived invasive species. Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can be successfully detected in samples from aquaria and an outdoor pond. We also conduct a cost comparison between eDNA detection and detection through traditional survey methods, using data from field surveys at two sites in our target area. We find that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2-10X higher than eDNA detection for the species in our study range. We summarize necessary future steps for application of eDNA surveys to turtle monitoring and conservation and propose specific cases in which the application of eDNA could further the conservation of threatened turtle species. |
| doi_str_mv | 10.1371/journal.pone.0130965 |
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Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can be successfully detected in samples from aquaria and an outdoor pond. We also conduct a cost comparison between eDNA detection and detection through traditional survey methods, using data from field surveys at two sites in our target area. We find that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2-10X higher than eDNA detection for the species in our study range. We summarize necessary future steps for application of eDNA surveys to turtle monitoring and conservation and propose specific cases in which the application of eDNA could further the conservation of threatened turtle species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0130965</identifier><identifier>PMID: 26200348</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Apalone spinifera ; Aquariums ; Biological monitoring ; Chelonia mydas ; Chelydra serpentina ; Chrysemys picta bellii ; Clemmys guttata ; Conservation ; Deoxyribonucleic acid ; Dermatemys mawii ; DNA ; DNA - genetics ; Ecosystem ; Emydoidea blandingii ; Emys orbicularis ; Endangered & extinct species ; Endangered Species ; Environmental DNA ; Environmental monitoring ; Environmental Monitoring - economics ; Environmental Monitoring - methods ; Glyptemys insculpta ; Introduced species ; Invasive species ; Natural resources ; Polls & surveys ; Polymerase chain reaction ; Primers ; Rafetus swinhoei ; Rare species ; Reptiles & amphibians ; Species Specificity ; Sternotherus odoratus ; Sympatric populations ; Testudines ; Threatened species ; Turtles ; Turtles - genetics ; Turtles - physiology ; Wildlife conservation</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0130965-e0130965</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Davy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Davy et al 2015 Davy et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3c738bb029095ff649a642530d25c3344295d1ad84b428099221b7d1ff298aad3</citedby><cites>FETCH-LOGICAL-c692t-3c738bb029095ff649a642530d25c3344295d1ad84b428099221b7d1ff298aad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511736/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511736/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26200348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mahon, Andrew R.</contributor><creatorcontrib>Davy, Christina M</creatorcontrib><creatorcontrib>Kidd, Anne G</creatorcontrib><creatorcontrib>Wilson, Chris C</creatorcontrib><title>Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Environmental DNA (eDNA) is a potentially powerful tool for detection and monitoring of rare species, including threatened native species and recently arrived invasive species. Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can be successfully detected in samples from aquaria and an outdoor pond. We also conduct a cost comparison between eDNA detection and detection through traditional survey methods, using data from field surveys at two sites in our target area. We find that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2-10X higher than eDNA detection for the species in our study range. We summarize necessary future steps for application of eDNA surveys to turtle monitoring and conservation and propose specific cases in which the application of eDNA could further the conservation of threatened turtle species.</description><subject>Analysis</subject><subject>Animals</subject><subject>Apalone spinifera</subject><subject>Aquariums</subject><subject>Biological monitoring</subject><subject>Chelonia mydas</subject><subject>Chelydra serpentina</subject><subject>Chrysemys picta bellii</subject><subject>Clemmys guttata</subject><subject>Conservation</subject><subject>Deoxyribonucleic acid</subject><subject>Dermatemys mawii</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Ecosystem</subject><subject>Emydoidea blandingii</subject><subject>Emys orbicularis</subject><subject>Endangered & extinct species</subject><subject>Endangered Species</subject><subject>Environmental DNA</subject><subject>Environmental monitoring</subject><subject>Environmental Monitoring - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davy, Christina M</au><au>Kidd, Anne G</au><au>Wilson, Chris C</au><au>Mahon, Andrew R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-22</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0130965</spage><epage>e0130965</epage><pages>e0130965-e0130965</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Environmental DNA (eDNA) is a potentially powerful tool for detection and monitoring of rare species, including threatened native species and recently arrived invasive species. Here, we develop DNA primers for a suite of nine sympatric freshwater turtles, and use it to test whether turtle eDNA can be successfully detected in samples from aquaria and an outdoor pond. We also conduct a cost comparison between eDNA detection and detection through traditional survey methods, using data from field surveys at two sites in our target area. We find that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2-10X higher than eDNA detection for the species in our study range. We summarize necessary future steps for application of eDNA surveys to turtle monitoring and conservation and propose specific cases in which the application of eDNA could further the conservation of threatened turtle species.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26200348</pmid><doi>10.1371/journal.pone.0130965</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Animals Apalone spinifera Aquariums Biological monitoring Chelonia mydas Chelydra serpentina Chrysemys picta bellii Clemmys guttata Conservation Deoxyribonucleic acid Dermatemys mawii DNA DNA - genetics Ecosystem Emydoidea blandingii Emys orbicularis Endangered & extinct species Endangered Species Environmental DNA Environmental monitoring Environmental Monitoring - economics Environmental Monitoring - methods Glyptemys insculpta Introduced species Invasive species Natural resources Polls & surveys Polymerase chain reaction Primers Rafetus swinhoei Rare species Reptiles & amphibians Species Specificity Sternotherus odoratus Sympatric populations Testudines Threatened species Turtles Turtles - genetics Turtles - physiology Wildlife conservation |
| title | Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles |
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