Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR

More than two decades after its discovery, contaminant microbial DNA in PCR reagents continues to impact the sensitivity and integrity of broad-range PCR diagnostic techniques. This is particularly relevant to their use in the setting of human sepsis, where a successful diagnostic on blood samples n...

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Veröffentlicht in:PloS one 2015-07, Vol.10 (7), p.e0132954-e0132954
Hauptverfasser: Humphrey, Bruce, McLeod, Neil, Turner, Carrie, Sutton, J Mark, Dark, Paul M, Warhurst, Geoffrey
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Sprache:eng
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