Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring
Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable f...
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| Veröffentlicht in: | PloS one 2015-07, Vol.10 (7), p.e0132038-e0132038 |
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| creator | Mennitti, Laís Vales Oyama, Lila Missae de Oliveira, Juliana Lopez Hachul, Ana Claudia Losinskas Santamarina, Aline Boveto de Santana, Aline Alves Okuda, Marcos Hiromu Ribeiro, Eliane Beraldi Oller do Nascimento, Claudia Maria da Penha Pisani, Luciana Pellegrini |
| description | Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-α (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RT-PCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway. |
| doi_str_mv | 10.1371/journal.pone.0132038 |
| format | Article |
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The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-α (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RT-PCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0132038</identifier><identifier>PMID: 26147005</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipocytes ; Adiponectin ; Adipose tissue ; Adipose Tissue, White - metabolism ; Animals ; Breastfeeding & lactation ; Cardiovascular disease ; Cell division ; Cytokines ; Diet ; Dietary supplements ; Endotoxemia ; Fatty acids ; Female ; Fetuses ; Fructooligosaccharides ; Gene expression ; Hydrogenation ; Inflammation ; Inflammation - chemically induced ; Inflammation - metabolism ; Interleukin ; Interleukin 6 ; Intra-Abdominal Fat - metabolism ; Lactation ; Liver ; Liver - metabolism ; Metabolic disorders ; Microbiota ; Muscle, Skeletal - metabolism ; Muscles ; NF-kappa B - metabolism ; NF-κB protein ; Nutrition research ; Offspring ; Oligosaccharides - pharmacology ; Permeability ; Phosphorylation ; Polymerase chain reaction ; Prebiotics ; Pregnancy ; Pregnant women ; Prenatal Exposure Delayed Effects - blood ; Prenatal Exposure Delayed Effects - metabolism ; Progeny ; Protein expression ; Proteins ; Rats ; Rats, Wistar ; Receptors, Adiponectin - genetics ; Receptors, Adiponectin - metabolism ; Receptors, Interleukin-6 - genetics ; Receptors, Interleukin-6 - metabolism ; RNA ; Rodents ; Signal transduction ; Studies ; TLR4 protein ; Toll-like receptors ; Tumor necrosis factor ; Tumor Necrosis Factor-alpha - blood ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Vegetables ; Western blotting</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0132038-e0132038</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Mennitti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Mennitti et al 2015 Mennitti et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-9665db5351db56017c173bfa00183e46f04cf2020166181032694231ef8bce773</citedby><cites>FETCH-LOGICAL-c692t-9665db5351db56017c173bfa00183e46f04cf2020166181032694231ef8bce773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493056/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493056/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26147005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Andrews, Zane</contributor><creatorcontrib>Mennitti, Laís Vales</creatorcontrib><creatorcontrib>Oyama, Lila Missae</creatorcontrib><creatorcontrib>de Oliveira, Juliana Lopez</creatorcontrib><creatorcontrib>Hachul, Ana Claudia Losinskas</creatorcontrib><creatorcontrib>Santamarina, Aline Boveto</creatorcontrib><creatorcontrib>de Santana, Aline Alves</creatorcontrib><creatorcontrib>Okuda, Marcos Hiromu</creatorcontrib><creatorcontrib>Ribeiro, Eliane Beraldi</creatorcontrib><creatorcontrib>Oller do Nascimento, Claudia Maria da Penha</creatorcontrib><creatorcontrib>Pisani, Luciana Pellegrini</creatorcontrib><title>Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-α (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RT-PCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway.</description><subject>Adipocytes</subject><subject>Adiponectin</subject><subject>Adipose tissue</subject><subject>Adipose Tissue, White - metabolism</subject><subject>Animals</subject><subject>Breastfeeding & lactation</subject><subject>Cardiovascular disease</subject><subject>Cell division</subject><subject>Cytokines</subject><subject>Diet</subject><subject>Dietary supplements</subject><subject>Endotoxemia</subject><subject>Fatty acids</subject><subject>Female</subject><subject>Fetuses</subject><subject>Fructooligosaccharides</subject><subject>Gene expression</subject><subject>Hydrogenation</subject><subject>Inflammation</subject><subject>Inflammation - chemically induced</subject><subject>Inflammation - metabolism</subject><subject>Interleukin</subject><subject>Interleukin 6</subject><subject>Intra-Abdominal Fat - metabolism</subject><subject>Lactation</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Metabolic disorders</subject><subject>Microbiota</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscles</subject><subject>NF-kappa B - metabolism</subject><subject>NF-κB protein</subject><subject>Nutrition research</subject><subject>Offspring</subject><subject>Oligosaccharides - pharmacology</subject><subject>Permeability</subject><subject>Phosphorylation</subject><subject>Polymerase chain reaction</subject><subject>Prebiotics</subject><subject>Pregnancy</subject><subject>Pregnant women</subject><subject>Prenatal Exposure Delayed Effects - blood</subject><subject>Prenatal Exposure Delayed Effects - metabolism</subject><subject>Progeny</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Adiponectin - genetics</subject><subject>Receptors, Adiponectin - metabolism</subject><subject>Receptors, Interleukin-6 - genetics</subject><subject>Receptors, Interleukin-6 - metabolism</subject><subject>RNA</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>Studies</subject><subject>TLR4 protein</subject><subject>Toll-like receptors</subject><subject>Tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha - blood</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Vegetables</subject><subject>Western blotting</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBYmkDbRYo_Eie5QZrGV6WiIgrcWq5jp6kcu9gO0B_C_8Wh2dSgXaBISWw_73uOj32S5CmCM0QK9Gpre2e4nu2skTOICIakvJecoorglMbB_aP_k-SR91sIc1JS-jA5wRRlRRyeJr8_8iAHH7DqdzstO2kCD6014GcbNmCp28Yq14tgvQQXCL64BHXvWtOAT042hhuxB9zUYMHFqFtIXnsQLJgb4ST3so6oTedGad51PFi3B6vI9h5YBcJGAozSN-lS12CplN8N5o-TB4prL5-M37Pk67u3X64_pIvl-_n11SIVtMIhrSjN63VOchTfFKJCoIKsFYcQlURmVMFMKAwxRJSiEkGCaZVhgqQq10IWBTlLnh98d9p6NlbUMxSxHMOsoJGYH4ja8i2LyXXc7ZnlLfs7YV3DuAut0JIJWhKKSU2rCmeF4BVXkGKZcVLiiqAh2usxWr_uZC1iqR3XE9Ppimk3rLE_WJZVBOZDMhejgbPfe-kD61ovpNbcSNsf8q4ogVUe0fN_0Lt3N1INjxtojbIxrhhM2VWGYYGLrIKRmt1BxaeWXSvi9VNtnJ8ILieCyAT5KzS8957NV5__n11-m7Ivj9iN5DpsvNX9cO_8FMwOoHDWeyfVbZERZEP33FSDDd3Dxu6JsmfHB3QrumkX8gesEhKu</recordid><startdate>20150706</startdate><enddate>20150706</enddate><creator>Mennitti, Laís Vales</creator><creator>Oyama, Lila Missae</creator><creator>de Oliveira, Juliana Lopez</creator><creator>Hachul, Ana Claudia Losinskas</creator><creator>Santamarina, Aline Boveto</creator><creator>de Santana, Aline Alves</creator><creator>Okuda, Marcos Hiromu</creator><creator>Ribeiro, Eliane Beraldi</creator><creator>Oller do Nascimento, Claudia Maria da Penha</creator><creator>Pisani, Luciana Pellegrini</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150706</creationdate><title>Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring</title><author>Mennitti, Laís Vales ; Oyama, Lila Missae ; de Oliveira, Juliana Lopez ; Hachul, Ana Claudia Losinskas ; Santamarina, Aline Boveto ; de Santana, Aline Alves ; Okuda, Marcos Hiromu ; Ribeiro, Eliane Beraldi ; Oller do Nascimento, Claudia Maria da Penha ; Pisani, Luciana Pellegrini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-9665db5351db56017c173bfa00183e46f04cf2020166181032694231ef8bce773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adipocytes</topic><topic>Adiponectin</topic><topic>Adipose tissue</topic><topic>Adipose Tissue, White - metabolism</topic><topic>Animals</topic><topic>Breastfeeding & lactation</topic><topic>Cardiovascular disease</topic><topic>Cell division</topic><topic>Cytokines</topic><topic>Diet</topic><topic>Dietary supplements</topic><topic>Endotoxemia</topic><topic>Fatty acids</topic><topic>Female</topic><topic>Fetuses</topic><topic>Fructooligosaccharides</topic><topic>Gene expression</topic><topic>Hydrogenation</topic><topic>Inflammation</topic><topic>Inflammation - chemically induced</topic><topic>Inflammation - metabolism</topic><topic>Interleukin</topic><topic>Interleukin 6</topic><topic>Intra-Abdominal Fat - metabolism</topic><topic>Lactation</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Metabolic disorders</topic><topic>Microbiota</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscles</topic><topic>NF-kappa B - metabolism</topic><topic>NF-κB protein</topic><topic>Nutrition research</topic><topic>Offspring</topic><topic>Oligosaccharides - pharmacology</topic><topic>Permeability</topic><topic>Phosphorylation</topic><topic>Polymerase chain reaction</topic><topic>Prebiotics</topic><topic>Pregnancy</topic><topic>Pregnant women</topic><topic>Prenatal Exposure Delayed Effects - blood</topic><topic>Prenatal Exposure Delayed Effects - metabolism</topic><topic>Progeny</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Adiponectin - genetics</topic><topic>Receptors, Adiponectin - metabolism</topic><topic>Receptors, Interleukin-6 - genetics</topic><topic>Receptors, Interleukin-6 - metabolism</topic><topic>RNA</topic><topic>Rodents</topic><topic>Signal transduction</topic><topic>Studies</topic><topic>TLR4 protein</topic><topic>Toll-like receptors</topic><topic>Tumor necrosis factor</topic><topic>Tumor Necrosis Factor-alpha - blood</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Vegetables</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mennitti, Laís Vales</creatorcontrib><creatorcontrib>Oyama, Lila Missae</creatorcontrib><creatorcontrib>de Oliveira, Juliana Lopez</creatorcontrib><creatorcontrib>Hachul, Ana Claudia Losinskas</creatorcontrib><creatorcontrib>Santamarina, Aline Boveto</creatorcontrib><creatorcontrib>de Santana, Aline Alves</creatorcontrib><creatorcontrib>Okuda, Marcos Hiromu</creatorcontrib><creatorcontrib>Ribeiro, Eliane Beraldi</creatorcontrib><creatorcontrib>Oller do Nascimento, Claudia Maria da Penha</creatorcontrib><creatorcontrib>Pisani, Luciana Pellegrini</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mennitti, Laís Vales</au><au>Oyama, Lila Missae</au><au>de Oliveira, Juliana Lopez</au><au>Hachul, Ana Claudia Losinskas</au><au>Santamarina, Aline Boveto</au><au>de Santana, Aline Alves</au><au>Okuda, Marcos Hiromu</au><au>Ribeiro, Eliane Beraldi</au><au>Oller do Nascimento, Claudia Maria da Penha</au><au>Pisani, Luciana Pellegrini</au><au>Andrews, Zane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-06</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0132038</spage><epage>e0132038</epage><pages>e0132038-e0132038</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-α (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RT-PCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased ADIPOR2 (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/ SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-α, protein expression of p-NFκBp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26147005</pmid><doi>10.1371/journal.pone.0132038</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-07, Vol.10 (7), p.e0132038-e0132038 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1694520476 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adipocytes Adiponectin Adipose tissue Adipose Tissue, White - metabolism Animals Breastfeeding & lactation Cardiovascular disease Cell division Cytokines Diet Dietary supplements Endotoxemia Fatty acids Female Fetuses Fructooligosaccharides Gene expression Hydrogenation Inflammation Inflammation - chemically induced Inflammation - metabolism Interleukin Interleukin 6 Intra-Abdominal Fat - metabolism Lactation Liver Liver - metabolism Metabolic disorders Microbiota Muscle, Skeletal - metabolism Muscles NF-kappa B - metabolism NF-κB protein Nutrition research Offspring Oligosaccharides - pharmacology Permeability Phosphorylation Polymerase chain reaction Prebiotics Pregnancy Pregnant women Prenatal Exposure Delayed Effects - blood Prenatal Exposure Delayed Effects - metabolism Progeny Protein expression Proteins Rats Rats, Wistar Receptors, Adiponectin - genetics Receptors, Adiponectin - metabolism Receptors, Interleukin-6 - genetics Receptors, Interleukin-6 - metabolism RNA Rodents Signal transduction Studies TLR4 protein Toll-like receptors Tumor necrosis factor Tumor Necrosis Factor-alpha - blood Tumor necrosis factor-TNF Tumor necrosis factor-α Vegetables Western blotting |
| title | Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring |
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