Rebamipide Promotes the Regeneration of Aspirin-Induced Small-Intestine Mucosal Injury through Accumulation of β-Catenin
The effect of rebamipide on repairing intestinal mucosal damage induced by nonsteroidal anti-inflammatory drugs and its mechanism remain unclear. In this study, we sought to explore the mechanism whereby rebamipide could promote the regeneration of aspirin-induced intestinal mucosal damage. BALB/c m...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-07, Vol.10 (7), p.e0132031-e0132031 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0132031 |
|---|---|
| container_issue | 7 |
| container_start_page | e0132031 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Lai, Yu Zhong, Wa Yu, Tao Xia, Zhong-Sheng Li, Jie-Yao Ouyang, Hui Shan, Ti-Dong Yang, Hong-Sheng Chen, Qi-Kui |
| description | The effect of rebamipide on repairing intestinal mucosal damage induced by nonsteroidal anti-inflammatory drugs and its mechanism remain unclear. In this study, we sought to explore the mechanism whereby rebamipide could promote the regeneration of aspirin-induced intestinal mucosal damage.
BALB/c mice were administered aspirin (200 mg/kg/d) for 5 days to induce acute small intestinal injury (SII). Subsequently, SII mice were treated with rebamipide (320 mg/kg/d) for 5 days. The structure of intestinal barrier was observed with transmission electron microscope, and Zo-1 and occludin expressions were detected. The proliferative index was indicated by the percentage of proliferating cell nuclear antigen positive cells. The prostaglandin E2 (PGE2) levels in the small intestine tissues were measured by an enzyme immunoassay. The mRNA and protein expression levels of cyclooxygenase (COX) and β-catenin signal were detected in the small intestine using quantitative PCR and Western blot, respectively.
COX expression was significantly down-regulated in aspirin induced SII (P < 0.05). In SII mice treated with rebamipide, histopathological findings of aspirin-induced intestinal inflammation were significantly milder and tight junctions between intestinal epithelial cells were improved significantly. The proliferative index increased after rebamipide treatment when compared with that in the control mice. The expressions of COX-2, β-catenin, and c-myc and the PGE2 concentrations in small intestinal tissues were significantly increased in mice with rebamipide treatments (P < 0.05).
Rebamipide administration in aspirin-induced SII mice could improve the intestinal barrier structure and promote the regeneration of small intestinal epithelial injury through up-regulating COX-2 expression and the accumulation of β-catenin. |
| doi_str_mv | 10.1371/journal.pone.0132031 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1693189115</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_76ece226ed704d3cb950528c1d94633f</doaj_id><sourcerecordid>3733337421</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-5af2c68a3c5a015e81a8d8ae323592d60286c90465bf704a0ece0609b359908d3</originalsourceid><addsrcrecordid>eNptkstuEzEUhkcIREvhDRCM1A2bCb6MHXuDFEVcIhWBCqwtxz6TOPLYwZ5BymvxIDwTbjONWsTKl_Of71z0V9VLjGaYzvHbXRxT0H62jwFmCFOCKH5UnWNJScPL4_G9-1n1LOcdQowKzp9WZ4RjyjAR59XhGta6d3tnof6aYh8HyPWwhfoaNhAg6cHFUMeuXuS9Sy40q2BHA7b-1mvvy6voBxeg_jyamLWvV2E3pkNBpDhutvXCmLEf_Qnz53ez1AMEF55XTzrtM7yYzovqx4f335efmqsvH1fLxVVjGOFDw3RHDBeaGqYRZiCwFlZooIQySSxHRHAjUcvZupujViMwgDiS6xKWSFh6Ub0-cvc-ZjVtLSvMJcVCYsyKYnVU2Kh3ap9cr9NBRe3U7UdMG6XT4IwHNecFTwgHW2pZataSIUaEwVa2nNKusN5N1cZ1D9ZAGJL2D6API8Ft1Sb-Um0rpGhxAbyZACn-HMtyVe-yAe91gDje9t1KziQWRXr5j_T_07VHlUkx5wTdqRmM1I2T7rLUjZPU5KSS9ur-IKekO-vQv0C5yKc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1693189115</pqid></control><display><type>article</type><title>Rebamipide Promotes the Regeneration of Aspirin-Induced Small-Intestine Mucosal Injury through Accumulation of β-Catenin</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Lai, Yu ; Zhong, Wa ; Yu, Tao ; Xia, Zhong-Sheng ; Li, Jie-Yao ; Ouyang, Hui ; Shan, Ti-Dong ; Yang, Hong-Sheng ; Chen, Qi-Kui</creator><contributor>Tasken, Kjetil</contributor><creatorcontrib>Lai, Yu ; Zhong, Wa ; Yu, Tao ; Xia, Zhong-Sheng ; Li, Jie-Yao ; Ouyang, Hui ; Shan, Ti-Dong ; Yang, Hong-Sheng ; Chen, Qi-Kui ; Tasken, Kjetil</creatorcontrib><description>The effect of rebamipide on repairing intestinal mucosal damage induced by nonsteroidal anti-inflammatory drugs and its mechanism remain unclear. In this study, we sought to explore the mechanism whereby rebamipide could promote the regeneration of aspirin-induced intestinal mucosal damage.
BALB/c mice were administered aspirin (200 mg/kg/d) for 5 days to induce acute small intestinal injury (SII). Subsequently, SII mice were treated with rebamipide (320 mg/kg/d) for 5 days. The structure of intestinal barrier was observed with transmission electron microscope, and Zo-1 and occludin expressions were detected. The proliferative index was indicated by the percentage of proliferating cell nuclear antigen positive cells. The prostaglandin E2 (PGE2) levels in the small intestine tissues were measured by an enzyme immunoassay. The mRNA and protein expression levels of cyclooxygenase (COX) and β-catenin signal were detected in the small intestine using quantitative PCR and Western blot, respectively.
COX expression was significantly down-regulated in aspirin induced SII (P < 0.05). In SII mice treated with rebamipide, histopathological findings of aspirin-induced intestinal inflammation were significantly milder and tight junctions between intestinal epithelial cells were improved significantly. The proliferative index increased after rebamipide treatment when compared with that in the control mice. The expressions of COX-2, β-catenin, and c-myc and the PGE2 concentrations in small intestinal tissues were significantly increased in mice with rebamipide treatments (P < 0.05).
Rebamipide administration in aspirin-induced SII mice could improve the intestinal barrier structure and promote the regeneration of small intestinal epithelial injury through up-regulating COX-2 expression and the accumulation of β-catenin.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0132031</identifier><identifier>PMID: 26135128</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Alanine - analogs & derivatives ; Alanine - pharmacology ; Animal tissues ; Animals ; Anti-inflammatory agents ; Anti-Inflammatory Agents, Non-Steroidal - toxicity ; Aspirin ; Aspirin - toxicity ; beta Catenin - biosynthesis ; beta Catenin - genetics ; beta Catenin - physiology ; Body Weight - drug effects ; c-Myc protein ; Cardiovascular disease ; Cell Division - drug effects ; Cyclooxygenase 2 - biosynthesis ; Cyclooxygenase 2 - genetics ; Cyclooxygenase-2 ; Dinoprostone - biosynthesis ; Drugs ; Enzyme immunoassay ; Epithelial cells ; Female ; Gastroenterology ; Gene expression ; Immunoassay ; Inflammation ; Injuries ; Intestinal Absorption - drug effects ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - physiology ; Intestinal Mucosa - ultrastructure ; Jejunum - drug effects ; Jejunum - physiology ; Jejunum - ultrastructure ; Laboratory animals ; Maintenance ; Male ; Membrane Proteins - biosynthesis ; Mice ; Mice, Inbred BALB C ; mRNA ; Mucosa ; Myc protein ; Nonsteroidal anti-inflammatory drugs ; Pathogenesis ; Permeability ; Proliferating cell nuclear antigen ; Prostaglandin E2 ; Proto-Oncogene Proteins c-myc - biosynthesis ; Quinolones - pharmacology ; Random Allocation ; Regeneration ; Regeneration - drug effects ; RNA, Messenger - biosynthesis ; Rodents ; Small intestine ; Studies ; Tight junctions ; Tight Junctions - drug effects ; Zonula occludens-1 protein ; β-Catenin</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0132031-e0132031</ispartof><rights>2015 Lai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Lai et al 2015 Lai et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-5af2c68a3c5a015e81a8d8ae323592d60286c90465bf704a0ece0609b359908d3</citedby><cites>FETCH-LOGICAL-c526t-5af2c68a3c5a015e81a8d8ae323592d60286c90465bf704a0ece0609b359908d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489841/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489841/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26135128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tasken, Kjetil</contributor><creatorcontrib>Lai, Yu</creatorcontrib><creatorcontrib>Zhong, Wa</creatorcontrib><creatorcontrib>Yu, Tao</creatorcontrib><creatorcontrib>Xia, Zhong-Sheng</creatorcontrib><creatorcontrib>Li, Jie-Yao</creatorcontrib><creatorcontrib>Ouyang, Hui</creatorcontrib><creatorcontrib>Shan, Ti-Dong</creatorcontrib><creatorcontrib>Yang, Hong-Sheng</creatorcontrib><creatorcontrib>Chen, Qi-Kui</creatorcontrib><title>Rebamipide Promotes the Regeneration of Aspirin-Induced Small-Intestine Mucosal Injury through Accumulation of β-Catenin</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The effect of rebamipide on repairing intestinal mucosal damage induced by nonsteroidal anti-inflammatory drugs and its mechanism remain unclear. In this study, we sought to explore the mechanism whereby rebamipide could promote the regeneration of aspirin-induced intestinal mucosal damage.
BALB/c mice were administered aspirin (200 mg/kg/d) for 5 days to induce acute small intestinal injury (SII). Subsequently, SII mice were treated with rebamipide (320 mg/kg/d) for 5 days. The structure of intestinal barrier was observed with transmission electron microscope, and Zo-1 and occludin expressions were detected. The proliferative index was indicated by the percentage of proliferating cell nuclear antigen positive cells. The prostaglandin E2 (PGE2) levels in the small intestine tissues were measured by an enzyme immunoassay. The mRNA and protein expression levels of cyclooxygenase (COX) and β-catenin signal were detected in the small intestine using quantitative PCR and Western blot, respectively.
COX expression was significantly down-regulated in aspirin induced SII (P < 0.05). In SII mice treated with rebamipide, histopathological findings of aspirin-induced intestinal inflammation were significantly milder and tight junctions between intestinal epithelial cells were improved significantly. The proliferative index increased after rebamipide treatment when compared with that in the control mice. The expressions of COX-2, β-catenin, and c-myc and the PGE2 concentrations in small intestinal tissues were significantly increased in mice with rebamipide treatments (P < 0.05).
Rebamipide administration in aspirin-induced SII mice could improve the intestinal barrier structure and promote the regeneration of small intestinal epithelial injury through up-regulating COX-2 expression and the accumulation of β-catenin.</description><subject>Accumulation</subject><subject>Alanine - analogs & derivatives</subject><subject>Alanine - pharmacology</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Anti-inflammatory agents</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - toxicity</subject><subject>Aspirin</subject><subject>Aspirin - toxicity</subject><subject>beta Catenin - biosynthesis</subject><subject>beta Catenin - genetics</subject><subject>beta Catenin - physiology</subject><subject>Body Weight - drug effects</subject><subject>c-Myc protein</subject><subject>Cardiovascular disease</subject><subject>Cell Division - drug effects</subject><subject>Cyclooxygenase 2 - biosynthesis</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cyclooxygenase-2</subject><subject>Dinoprostone - biosynthesis</subject><subject>Drugs</subject><subject>Enzyme immunoassay</subject><subject>Epithelial cells</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Gene expression</subject><subject>Immunoassay</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Intestinal Absorption - drug effects</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - physiology</subject><subject>Intestinal Mucosa - ultrastructure</subject><subject>Jejunum - drug effects</subject><subject>Jejunum - physiology</subject><subject>Jejunum - ultrastructure</subject><subject>Laboratory animals</subject><subject>Maintenance</subject><subject>Male</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>mRNA</subject><subject>Mucosa</subject><subject>Myc protein</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>Pathogenesis</subject><subject>Permeability</subject><subject>Proliferating cell nuclear antigen</subject><subject>Prostaglandin E2</subject><subject>Proto-Oncogene Proteins c-myc - biosynthesis</subject><subject>Quinolones - pharmacology</subject><subject>Random Allocation</subject><subject>Regeneration</subject><subject>Regeneration - drug effects</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Rodents</subject><subject>Small intestine</subject><subject>Studies</subject><subject>Tight junctions</subject><subject>Tight Junctions - drug effects</subject><subject>Zonula occludens-1 protein</subject><subject>β-Catenin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkstuEzEUhkcIREvhDRCM1A2bCb6MHXuDFEVcIhWBCqwtxz6TOPLYwZ5BymvxIDwTbjONWsTKl_Of71z0V9VLjGaYzvHbXRxT0H62jwFmCFOCKH5UnWNJScPL4_G9-1n1LOcdQowKzp9WZ4RjyjAR59XhGta6d3tnof6aYh8HyPWwhfoaNhAg6cHFUMeuXuS9Sy40q2BHA7b-1mvvy6voBxeg_jyamLWvV2E3pkNBpDhutvXCmLEf_Qnz53ez1AMEF55XTzrtM7yYzovqx4f335efmqsvH1fLxVVjGOFDw3RHDBeaGqYRZiCwFlZooIQySSxHRHAjUcvZupujViMwgDiS6xKWSFh6Ub0-cvc-ZjVtLSvMJcVCYsyKYnVU2Kh3ap9cr9NBRe3U7UdMG6XT4IwHNecFTwgHW2pZataSIUaEwVa2nNKusN5N1cZ1D9ZAGJL2D6API8Ft1Sb-Um0rpGhxAbyZACn-HMtyVe-yAe91gDje9t1KziQWRXr5j_T_07VHlUkx5wTdqRmM1I2T7rLUjZPU5KSS9ur-IKekO-vQv0C5yKc</recordid><startdate>20150702</startdate><enddate>20150702</enddate><creator>Lai, Yu</creator><creator>Zhong, Wa</creator><creator>Yu, Tao</creator><creator>Xia, Zhong-Sheng</creator><creator>Li, Jie-Yao</creator><creator>Ouyang, Hui</creator><creator>Shan, Ti-Dong</creator><creator>Yang, Hong-Sheng</creator><creator>Chen, Qi-Kui</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150702</creationdate><title>Rebamipide Promotes the Regeneration of Aspirin-Induced Small-Intestine Mucosal Injury through Accumulation of β-Catenin</title><author>Lai, Yu ; Zhong, Wa ; Yu, Tao ; Xia, Zhong-Sheng ; Li, Jie-Yao ; Ouyang, Hui ; Shan, Ti-Dong ; Yang, Hong-Sheng ; Chen, Qi-Kui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-5af2c68a3c5a015e81a8d8ae323592d60286c90465bf704a0ece0609b359908d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Accumulation</topic><topic>Alanine - analogs & derivatives</topic><topic>Alanine - pharmacology</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Anti-inflammatory agents</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - toxicity</topic><topic>Aspirin</topic><topic>Aspirin - toxicity</topic><topic>beta Catenin - biosynthesis</topic><topic>beta Catenin - genetics</topic><topic>beta Catenin - physiology</topic><topic>Body Weight - drug effects</topic><topic>c-Myc protein</topic><topic>Cardiovascular disease</topic><topic>Cell Division - drug effects</topic><topic>Cyclooxygenase 2 - biosynthesis</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cyclooxygenase-2</topic><topic>Dinoprostone - biosynthesis</topic><topic>Drugs</topic><topic>Enzyme immunoassay</topic><topic>Epithelial cells</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>Gene expression</topic><topic>Immunoassay</topic><topic>Inflammation</topic><topic>Injuries</topic><topic>Intestinal Absorption - drug effects</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - physiology</topic><topic>Intestinal Mucosa - ultrastructure</topic><topic>Jejunum - drug effects</topic><topic>Jejunum - physiology</topic><topic>Jejunum - ultrastructure</topic><topic>Laboratory animals</topic><topic>Maintenance</topic><topic>Male</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>mRNA</topic><topic>Mucosa</topic><topic>Myc protein</topic><topic>Nonsteroidal anti-inflammatory drugs</topic><topic>Pathogenesis</topic><topic>Permeability</topic><topic>Proliferating cell nuclear antigen</topic><topic>Prostaglandin E2</topic><topic>Proto-Oncogene Proteins c-myc - biosynthesis</topic><topic>Quinolones - pharmacology</topic><topic>Random Allocation</topic><topic>Regeneration</topic><topic>Regeneration - drug effects</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Rodents</topic><topic>Small intestine</topic><topic>Studies</topic><topic>Tight junctions</topic><topic>Tight Junctions - drug effects</topic><topic>Zonula occludens-1 protein</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lai, Yu</creatorcontrib><creatorcontrib>Zhong, Wa</creatorcontrib><creatorcontrib>Yu, Tao</creatorcontrib><creatorcontrib>Xia, Zhong-Sheng</creatorcontrib><creatorcontrib>Li, Jie-Yao</creatorcontrib><creatorcontrib>Ouyang, Hui</creatorcontrib><creatorcontrib>Shan, Ti-Dong</creatorcontrib><creatorcontrib>Yang, Hong-Sheng</creatorcontrib><creatorcontrib>Chen, Qi-Kui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lai, Yu</au><au>Zhong, Wa</au><au>Yu, Tao</au><au>Xia, Zhong-Sheng</au><au>Li, Jie-Yao</au><au>Ouyang, Hui</au><au>Shan, Ti-Dong</au><au>Yang, Hong-Sheng</au><au>Chen, Qi-Kui</au><au>Tasken, Kjetil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rebamipide Promotes the Regeneration of Aspirin-Induced Small-Intestine Mucosal Injury through Accumulation of β-Catenin</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-02</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0132031</spage><epage>e0132031</epage><pages>e0132031-e0132031</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The effect of rebamipide on repairing intestinal mucosal damage induced by nonsteroidal anti-inflammatory drugs and its mechanism remain unclear. In this study, we sought to explore the mechanism whereby rebamipide could promote the regeneration of aspirin-induced intestinal mucosal damage.
BALB/c mice were administered aspirin (200 mg/kg/d) for 5 days to induce acute small intestinal injury (SII). Subsequently, SII mice were treated with rebamipide (320 mg/kg/d) for 5 days. The structure of intestinal barrier was observed with transmission electron microscope, and Zo-1 and occludin expressions were detected. The proliferative index was indicated by the percentage of proliferating cell nuclear antigen positive cells. The prostaglandin E2 (PGE2) levels in the small intestine tissues were measured by an enzyme immunoassay. The mRNA and protein expression levels of cyclooxygenase (COX) and β-catenin signal were detected in the small intestine using quantitative PCR and Western blot, respectively.
COX expression was significantly down-regulated in aspirin induced SII (P < 0.05). In SII mice treated with rebamipide, histopathological findings of aspirin-induced intestinal inflammation were significantly milder and tight junctions between intestinal epithelial cells were improved significantly. The proliferative index increased after rebamipide treatment when compared with that in the control mice. The expressions of COX-2, β-catenin, and c-myc and the PGE2 concentrations in small intestinal tissues were significantly increased in mice with rebamipide treatments (P < 0.05).
Rebamipide administration in aspirin-induced SII mice could improve the intestinal barrier structure and promote the regeneration of small intestinal epithelial injury through up-regulating COX-2 expression and the accumulation of β-catenin.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26135128</pmid><doi>10.1371/journal.pone.0132031</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-07, Vol.10 (7), p.e0132031-e0132031 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1693189115 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Accumulation Alanine - analogs & derivatives Alanine - pharmacology Animal tissues Animals Anti-inflammatory agents Anti-Inflammatory Agents, Non-Steroidal - toxicity Aspirin Aspirin - toxicity beta Catenin - biosynthesis beta Catenin - genetics beta Catenin - physiology Body Weight - drug effects c-Myc protein Cardiovascular disease Cell Division - drug effects Cyclooxygenase 2 - biosynthesis Cyclooxygenase 2 - genetics Cyclooxygenase-2 Dinoprostone - biosynthesis Drugs Enzyme immunoassay Epithelial cells Female Gastroenterology Gene expression Immunoassay Inflammation Injuries Intestinal Absorption - drug effects Intestinal Mucosa - drug effects Intestinal Mucosa - physiology Intestinal Mucosa - ultrastructure Jejunum - drug effects Jejunum - physiology Jejunum - ultrastructure Laboratory animals Maintenance Male Membrane Proteins - biosynthesis Mice Mice, Inbred BALB C mRNA Mucosa Myc protein Nonsteroidal anti-inflammatory drugs Pathogenesis Permeability Proliferating cell nuclear antigen Prostaglandin E2 Proto-Oncogene Proteins c-myc - biosynthesis Quinolones - pharmacology Random Allocation Regeneration Regeneration - drug effects RNA, Messenger - biosynthesis Rodents Small intestine Studies Tight junctions Tight Junctions - drug effects Zonula occludens-1 protein β-Catenin |
| title | Rebamipide Promotes the Regeneration of Aspirin-Induced Small-Intestine Mucosal Injury through Accumulation of β-Catenin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T06%3A37%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rebamipide%20Promotes%20the%20Regeneration%20of%20Aspirin-Induced%20Small-Intestine%20Mucosal%20Injury%20through%20Accumulation%20of%20%CE%B2-Catenin&rft.jtitle=PloS%20one&rft.au=Lai,%20Yu&rft.date=2015-07-02&rft.volume=10&rft.issue=7&rft.spage=e0132031&rft.epage=e0132031&rft.pages=e0132031-e0132031&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0132031&rft_dat=%3Cproquest_plos_%3E3733337421%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1693189115&rft_id=info:pmid/26135128&rft_doaj_id=oai_doaj_org_article_76ece226ed704d3cb950528c1d94633f&rfr_iscdi=true |