Placental Features of Late-Onset Adverse Pregnancy Outcome
Currently, no investigations reliably identify placental dysfunction in late pregnancy. To facilitate the development of such investigations we aimed to identify placental features that differ between normal and adverse outcome in late pregnancy in a group of pregnancies with reduced fetal movement....
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-06, Vol.10 (6), p.e0129117-e0129117 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0129117 |
|---|---|
| container_issue | 6 |
| container_start_page | e0129117 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Higgins, Lucy E Rey de Castro, Nicolas Addo, Naa Wareing, Mark Greenwood, Susan L Jones, Rebecca L Sibley, Colin P Johnstone, Edward D Heazell, Alexander E P |
| description | Currently, no investigations reliably identify placental dysfunction in late pregnancy. To facilitate the development of such investigations we aimed to identify placental features that differ between normal and adverse outcome in late pregnancy in a group of pregnancies with reduced fetal movement.
Following third trimester presentation with reduced fetal movement (N = 100), placental structure ex vivo was measured. Placental function was then assessed in terms of (i) chorionic plate artery agonist responses and length-tension characteristics using wire myography and (ii) production and release of placentally derived hormones (by quantitative polymerase chain reaction and enzyme linked immunosorbant assay of villous tissue and explant conditioned culture medium).
Placentas from pregnancies ending in adverse outcome (N = 23) were ~25% smaller in weight, volume, length, width and disc area (all p |
| doi_str_mv | 10.1371/journal.pone.0129117 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1692019660</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_dd7539db0ead422ea16b8030a0318776</doaj_id><sourcerecordid>3729805921</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-3107f0ac7249493c6a8912215c7deb05891cb74be8b904d1d6fb2e66604cd5383</originalsourceid><addsrcrecordid>eNptUstu1DAUtRAVLYU_QBCJDZtM_YofLJCqij6kkaaLsrYc-2bIyBMPdlKpf4-HSasWdeXXueee43sQ-kTwgjBJzjZxSoMNi10cYIEJ1YTIN-iEaEZrQTF7-2x_jN7nvMG4YUqId-iYCkKxYuoEfb8N1sEw2lBdgh2nBLmKXbW0I9SrIcNYnft7SBmq2wTrwQ7uoVpNo4tb-ICOOhsyfJzXU_Tr8ufdxXW9XF3dXJwva9dQMdaMYNlh6yTlmmvmhFWaUEoaJz20uCkn10regmo15p540bUUhBCYO18Es1P05cC7CzGb2XY2RGiKiS64grg5IHy0G7NL_damBxNtb_5dxLQ2No29C2C8lw3TvsVgPacULBGtwgxbzIiSUhSuH3O3qd2C3_9NsuEF6cuXof9t1vHecK4UFbwQfJsJUvwzQR7Nts8OQrADxOmgWzZlXqxAv_4Hfd0dP6Bcijkn6J7EEGz2UXisMvsomDkKpezzcyNPRY-zZ38Bhryv8g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1692019660</pqid></control><display><type>article</type><title>Placental Features of Late-Onset Adverse Pregnancy Outcome</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Higgins, Lucy E ; Rey de Castro, Nicolas ; Addo, Naa ; Wareing, Mark ; Greenwood, Susan L ; Jones, Rebecca L ; Sibley, Colin P ; Johnstone, Edward D ; Heazell, Alexander E P</creator><contributor>Vatish, Manu</contributor><creatorcontrib>Higgins, Lucy E ; Rey de Castro, Nicolas ; Addo, Naa ; Wareing, Mark ; Greenwood, Susan L ; Jones, Rebecca L ; Sibley, Colin P ; Johnstone, Edward D ; Heazell, Alexander E P ; Vatish, Manu</creatorcontrib><description>Currently, no investigations reliably identify placental dysfunction in late pregnancy. To facilitate the development of such investigations we aimed to identify placental features that differ between normal and adverse outcome in late pregnancy in a group of pregnancies with reduced fetal movement.
Following third trimester presentation with reduced fetal movement (N = 100), placental structure ex vivo was measured. Placental function was then assessed in terms of (i) chorionic plate artery agonist responses and length-tension characteristics using wire myography and (ii) production and release of placentally derived hormones (by quantitative polymerase chain reaction and enzyme linked immunosorbant assay of villous tissue and explant conditioned culture medium).
Placentas from pregnancies ending in adverse outcome (N = 23) were ~25% smaller in weight, volume, length, width and disc area (all p<0.0001) compared with those from normal outcome pregnancies. Villous and trophoblast areas were unchanged, but villous vascularity was reduced (median (interquartile range): adverse outcome 10 (10-12) vessels/mm2 vs. normal outcome 13 (12-15), p = 0.002). Adverse outcome pregnancy placental arteries were relatively insensitive to nitric oxide donated by sodium nitroprusside compared to normal outcome pregnancy placental arteries (50% Effective Concentration 30 (19-50) nM vs. 12 (6-24), p = 0.02). Adverse outcome pregnancy placental tissue contained less human chorionic gonadotrophin (20 (11-50) vs. 55 (24-102) mIU/mg, p = 0.007) and human placental lactogen (11 (6-14) vs. 27 (9-50) mg/mg, p = 0.006) and released more soluble fms-like tyrosine kinase-1 (21 (13-29) vs. 5 (2-15) ng/mg, p = 0.01) compared with normal outcome pregnancy placental tissue.
These data provide a description of the placental phenotype of adverse outcome in late pregnancy. Antenatal tests that accurately reflect elements of this phenotype may improve its prediction.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0129117</identifier><identifier>PMID: 26120838</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Arteries ; Blood vessels ; Conditioning ; Endocrine System - pathology ; Endocrine System - physiopathology ; Female ; Fetal Growth Retardation - pathology ; Fetal Movement ; Fetuses ; Flexible manufacturing systems ; Growth factors ; Hormones ; Hospitals ; Humans ; Medical research ; Mortality ; Nitric oxide ; Phenotypes ; Pituitary (anterior) ; Placenta ; Placenta - blood supply ; Placenta - pathology ; Placenta - physiopathology ; Plates (structural members) ; Polymerase chain reaction ; Pregnancy ; Pregnancy Outcome ; Pregnancy Trimester, Third - physiology ; Protein-tyrosine kinase ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Science ; Sodium ; Sodium nitroprusside ; Stillbirth ; Systematic review ; Tissue culture ; Tyrosine</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0129117-e0129117</ispartof><rights>2015 Higgins et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Higgins et al 2015 Higgins et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-3107f0ac7249493c6a8912215c7deb05891cb74be8b904d1d6fb2e66604cd5383</citedby><cites>FETCH-LOGICAL-c526t-3107f0ac7249493c6a8912215c7deb05891cb74be8b904d1d6fb2e66604cd5383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488264/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488264/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26120838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Vatish, Manu</contributor><creatorcontrib>Higgins, Lucy E</creatorcontrib><creatorcontrib>Rey de Castro, Nicolas</creatorcontrib><creatorcontrib>Addo, Naa</creatorcontrib><creatorcontrib>Wareing, Mark</creatorcontrib><creatorcontrib>Greenwood, Susan L</creatorcontrib><creatorcontrib>Jones, Rebecca L</creatorcontrib><creatorcontrib>Sibley, Colin P</creatorcontrib><creatorcontrib>Johnstone, Edward D</creatorcontrib><creatorcontrib>Heazell, Alexander E P</creatorcontrib><title>Placental Features of Late-Onset Adverse Pregnancy Outcome</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Currently, no investigations reliably identify placental dysfunction in late pregnancy. To facilitate the development of such investigations we aimed to identify placental features that differ between normal and adverse outcome in late pregnancy in a group of pregnancies with reduced fetal movement.
Following third trimester presentation with reduced fetal movement (N = 100), placental structure ex vivo was measured. Placental function was then assessed in terms of (i) chorionic plate artery agonist responses and length-tension characteristics using wire myography and (ii) production and release of placentally derived hormones (by quantitative polymerase chain reaction and enzyme linked immunosorbant assay of villous tissue and explant conditioned culture medium).
Placentas from pregnancies ending in adverse outcome (N = 23) were ~25% smaller in weight, volume, length, width and disc area (all p<0.0001) compared with those from normal outcome pregnancies. Villous and trophoblast areas were unchanged, but villous vascularity was reduced (median (interquartile range): adverse outcome 10 (10-12) vessels/mm2 vs. normal outcome 13 (12-15), p = 0.002). Adverse outcome pregnancy placental arteries were relatively insensitive to nitric oxide donated by sodium nitroprusside compared to normal outcome pregnancy placental arteries (50% Effective Concentration 30 (19-50) nM vs. 12 (6-24), p = 0.02). Adverse outcome pregnancy placental tissue contained less human chorionic gonadotrophin (20 (11-50) vs. 55 (24-102) mIU/mg, p = 0.007) and human placental lactogen (11 (6-14) vs. 27 (9-50) mg/mg, p = 0.006) and released more soluble fms-like tyrosine kinase-1 (21 (13-29) vs. 5 (2-15) ng/mg, p = 0.01) compared with normal outcome pregnancy placental tissue.
These data provide a description of the placental phenotype of adverse outcome in late pregnancy. Antenatal tests that accurately reflect elements of this phenotype may improve its prediction.</description><subject>Adult</subject><subject>Arteries</subject><subject>Blood vessels</subject><subject>Conditioning</subject><subject>Endocrine System - pathology</subject><subject>Endocrine System - physiopathology</subject><subject>Female</subject><subject>Fetal Growth Retardation - pathology</subject><subject>Fetal Movement</subject><subject>Fetuses</subject><subject>Flexible manufacturing systems</subject><subject>Growth factors</subject><subject>Hormones</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Medical research</subject><subject>Mortality</subject><subject>Nitric oxide</subject><subject>Phenotypes</subject><subject>Pituitary (anterior)</subject><subject>Placenta</subject><subject>Placenta - blood supply</subject><subject>Placenta - pathology</subject><subject>Placenta - physiopathology</subject><subject>Plates (structural members)</subject><subject>Polymerase chain reaction</subject><subject>Pregnancy</subject><subject>Pregnancy Outcome</subject><subject>Pregnancy Trimester, Third - physiology</subject><subject>Protein-tyrosine kinase</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Science</subject><subject>Sodium</subject><subject>Sodium nitroprusside</subject><subject>Stillbirth</subject><subject>Systematic review</subject><subject>Tissue culture</subject><subject>Tyrosine</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUstu1DAUtRAVLYU_QBCJDZtM_YofLJCqij6kkaaLsrYc-2bIyBMPdlKpf4-HSasWdeXXueee43sQ-kTwgjBJzjZxSoMNi10cYIEJ1YTIN-iEaEZrQTF7-2x_jN7nvMG4YUqId-iYCkKxYuoEfb8N1sEw2lBdgh2nBLmKXbW0I9SrIcNYnft7SBmq2wTrwQ7uoVpNo4tb-ICOOhsyfJzXU_Tr8ufdxXW9XF3dXJwva9dQMdaMYNlh6yTlmmvmhFWaUEoaJz20uCkn10regmo15p540bUUhBCYO18Es1P05cC7CzGb2XY2RGiKiS64grg5IHy0G7NL_damBxNtb_5dxLQ2No29C2C8lw3TvsVgPacULBGtwgxbzIiSUhSuH3O3qd2C3_9NsuEF6cuXof9t1vHecK4UFbwQfJsJUvwzQR7Nts8OQrADxOmgWzZlXqxAv_4Hfd0dP6Bcijkn6J7EEGz2UXisMvsomDkKpezzcyNPRY-zZ38Bhryv8g</recordid><startdate>20150629</startdate><enddate>20150629</enddate><creator>Higgins, Lucy E</creator><creator>Rey de Castro, Nicolas</creator><creator>Addo, Naa</creator><creator>Wareing, Mark</creator><creator>Greenwood, Susan L</creator><creator>Jones, Rebecca L</creator><creator>Sibley, Colin P</creator><creator>Johnstone, Edward D</creator><creator>Heazell, Alexander E P</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150629</creationdate><title>Placental Features of Late-Onset Adverse Pregnancy Outcome</title><author>Higgins, Lucy E ; Rey de Castro, Nicolas ; Addo, Naa ; Wareing, Mark ; Greenwood, Susan L ; Jones, Rebecca L ; Sibley, Colin P ; Johnstone, Edward D ; Heazell, Alexander E P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-3107f0ac7249493c6a8912215c7deb05891cb74be8b904d1d6fb2e66604cd5383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Arteries</topic><topic>Blood vessels</topic><topic>Conditioning</topic><topic>Endocrine System - pathology</topic><topic>Endocrine System - physiopathology</topic><topic>Female</topic><topic>Fetal Growth Retardation - pathology</topic><topic>Fetal Movement</topic><topic>Fetuses</topic><topic>Flexible manufacturing systems</topic><topic>Growth factors</topic><topic>Hormones</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Medical research</topic><topic>Mortality</topic><topic>Nitric oxide</topic><topic>Phenotypes</topic><topic>Pituitary (anterior)</topic><topic>Placenta</topic><topic>Placenta - blood supply</topic><topic>Placenta - pathology</topic><topic>Placenta - physiopathology</topic><topic>Plates (structural members)</topic><topic>Polymerase chain reaction</topic><topic>Pregnancy</topic><topic>Pregnancy Outcome</topic><topic>Pregnancy Trimester, Third - physiology</topic><topic>Protein-tyrosine kinase</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Science</topic><topic>Sodium</topic><topic>Sodium nitroprusside</topic><topic>Stillbirth</topic><topic>Systematic review</topic><topic>Tissue culture</topic><topic>Tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Higgins, Lucy E</creatorcontrib><creatorcontrib>Rey de Castro, Nicolas</creatorcontrib><creatorcontrib>Addo, Naa</creatorcontrib><creatorcontrib>Wareing, Mark</creatorcontrib><creatorcontrib>Greenwood, Susan L</creatorcontrib><creatorcontrib>Jones, Rebecca L</creatorcontrib><creatorcontrib>Sibley, Colin P</creatorcontrib><creatorcontrib>Johnstone, Edward D</creatorcontrib><creatorcontrib>Heazell, Alexander E P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Higgins, Lucy E</au><au>Rey de Castro, Nicolas</au><au>Addo, Naa</au><au>Wareing, Mark</au><au>Greenwood, Susan L</au><au>Jones, Rebecca L</au><au>Sibley, Colin P</au><au>Johnstone, Edward D</au><au>Heazell, Alexander E P</au><au>Vatish, Manu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Placental Features of Late-Onset Adverse Pregnancy Outcome</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-29</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0129117</spage><epage>e0129117</epage><pages>e0129117-e0129117</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Currently, no investigations reliably identify placental dysfunction in late pregnancy. To facilitate the development of such investigations we aimed to identify placental features that differ between normal and adverse outcome in late pregnancy in a group of pregnancies with reduced fetal movement.
Following third trimester presentation with reduced fetal movement (N = 100), placental structure ex vivo was measured. Placental function was then assessed in terms of (i) chorionic plate artery agonist responses and length-tension characteristics using wire myography and (ii) production and release of placentally derived hormones (by quantitative polymerase chain reaction and enzyme linked immunosorbant assay of villous tissue and explant conditioned culture medium).
Placentas from pregnancies ending in adverse outcome (N = 23) were ~25% smaller in weight, volume, length, width and disc area (all p<0.0001) compared with those from normal outcome pregnancies. Villous and trophoblast areas were unchanged, but villous vascularity was reduced (median (interquartile range): adverse outcome 10 (10-12) vessels/mm2 vs. normal outcome 13 (12-15), p = 0.002). Adverse outcome pregnancy placental arteries were relatively insensitive to nitric oxide donated by sodium nitroprusside compared to normal outcome pregnancy placental arteries (50% Effective Concentration 30 (19-50) nM vs. 12 (6-24), p = 0.02). Adverse outcome pregnancy placental tissue contained less human chorionic gonadotrophin (20 (11-50) vs. 55 (24-102) mIU/mg, p = 0.007) and human placental lactogen (11 (6-14) vs. 27 (9-50) mg/mg, p = 0.006) and released more soluble fms-like tyrosine kinase-1 (21 (13-29) vs. 5 (2-15) ng/mg, p = 0.01) compared with normal outcome pregnancy placental tissue.
These data provide a description of the placental phenotype of adverse outcome in late pregnancy. Antenatal tests that accurately reflect elements of this phenotype may improve its prediction.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26120838</pmid><doi>10.1371/journal.pone.0129117</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-06, Vol.10 (6), p.e0129117-e0129117 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1692019660 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Arteries Blood vessels Conditioning Endocrine System - pathology Endocrine System - physiopathology Female Fetal Growth Retardation - pathology Fetal Movement Fetuses Flexible manufacturing systems Growth factors Hormones Hospitals Humans Medical research Mortality Nitric oxide Phenotypes Pituitary (anterior) Placenta Placenta - blood supply Placenta - pathology Placenta - physiopathology Plates (structural members) Polymerase chain reaction Pregnancy Pregnancy Outcome Pregnancy Trimester, Third - physiology Protein-tyrosine kinase RNA, Messenger - genetics RNA, Messenger - metabolism Science Sodium Sodium nitroprusside Stillbirth Systematic review Tissue culture Tyrosine |
| title | Placental Features of Late-Onset Adverse Pregnancy Outcome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T10%3A20%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Placental%20Features%20of%20Late-Onset%20Adverse%20Pregnancy%20Outcome&rft.jtitle=PloS%20one&rft.au=Higgins,%20Lucy%20E&rft.date=2015-06-29&rft.volume=10&rft.issue=6&rft.spage=e0129117&rft.epage=e0129117&rft.pages=e0129117-e0129117&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0129117&rft_dat=%3Cproquest_plos_%3E3729805921%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1692019660&rft_id=info:pmid/26120838&rft_doaj_id=oai_doaj_org_article_dd7539db0ead422ea16b8030a0318776&rfr_iscdi=true |