Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level
Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insig...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-06, Vol.10 (6), p.e0129937-e0129937 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0129937 |
|---|---|
| container_issue | 6 |
| container_start_page | e0129937 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Yan, Jing Liao, Kai Wang, Tianjiao Mai, Kangsen Xu, Wei Ai, Qinghui |
| description | Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how dietary lipids affect lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a diet with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 10 weeks. Growth performance, plasma biochemical indexes, lipid contents and gene expression related to lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-lipid diet. In the livers of fish fed the high-lipid diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control diet, and hepatic lipid deposition increased. In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet. However, hepatic lipid content in fish fed the low-lipid diet decreased mainly due to low dietary lipid intake. In summary, findings of this study provide molecular insight into the role of lipid deposition in the liver in response to different dietary lipid contents. |
| doi_str_mv | 10.1371/journal.pone.0129937 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1691409262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_76ce5765d1864f9aa330cdee724d854d</doaj_id><sourcerecordid>1692294418</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-f876cffd53e70812d5dc6971b131bf1219d8a84825cf00d8c4e443d589b58def3</originalsourceid><addsrcrecordid>eNptUttuEzEUXCEQLYU_QGCJlyKRsr7s7QWpSilEioRU0geeLK99NnFw7MV2gvab-Qm8SVq1iCdb4zlz5nhOlr3G-QWmFf64dltvhbnonYWLHJOmodWT7BQ3lExKktOnD-4n2YsQ1nle0Losn2cnpMSYMdKcZn-uNEThBzTXvVZoDjswAc1sZ7ZgJRzhK-hd0FE7i7RFcTXiO_DIdWgu_BLQDzDG_UZT78TPhJ8nVG-0XMXVEJD0ToJ4j9oB3cBya0TUdjkqu967CEnxBiT00fnwAV2LGAd0KVPX2z4mNSSsQguvhRzM0gwSvDPo-2CTi6DD_nUqomid0WGDRNzbW3hhg_S6Hz0Lc5jrZfasEybAq-N5lt1ef15Mv07m377MppfziSxIGSddXZWy61RBocprTFShZNlUuMUUtx0muFG1qFlNCtnluaolA8aoKuqmLWoFHT3L3h50e-MCP-YUOC4bzPKGlCQxZgeGcmLN-_RXKQLuhOZ7wPklFz5qaYAnL1BUZaFwXbKuEYLSXCqAijBVF0wlrU_Hbtt2A0qCjV6YR6KPX6xe8aXbcZZGqOho5vwo4N2vLYTINzrIFKiw4LZ734Q0jOE6Ud_9Q_3_dOzASsGH4KG7N4NzPu7uXRUfd5cfdzeVvXk4yH3R3bLSv8PS8pI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1691409262</pqid></control><display><type>article</type><title>Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Yan, Jing ; Liao, Kai ; Wang, Tianjiao ; Mai, Kangsen ; Xu, Wei ; Ai, Qinghui</creator><creatorcontrib>Yan, Jing ; Liao, Kai ; Wang, Tianjiao ; Mai, Kangsen ; Xu, Wei ; Ai, Qinghui</creatorcontrib><description>Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how dietary lipids affect lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a diet with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 10 weeks. Growth performance, plasma biochemical indexes, lipid contents and gene expression related to lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-lipid diet. In the livers of fish fed the high-lipid diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control diet, and hepatic lipid deposition increased. In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet. However, hepatic lipid content in fish fed the low-lipid diet decreased mainly due to low dietary lipid intake. In summary, findings of this study provide molecular insight into the role of lipid deposition in the liver in response to different dietary lipid contents.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0129937</identifier><identifier>PMID: 26114429</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Aquaculture ; Assembly ; Catabolism ; Catalysis ; CD36 antigen ; Cholesterol ; Cholesterol, VLDL - metabolism ; Deposition ; Diet ; Dietary intake ; Education ; Esterification ; Fatty acids ; Fatty Acids - metabolism ; Fish ; Fish oils ; Gene Expression ; Genes ; Laboratories ; Larimichthys crocea ; Lipid Metabolism ; Lipids ; Lipoprotein (low density) receptors ; Lipoprotein receptors ; Lipoproteins ; Liver ; Liver - metabolism ; Low density lipoprotein receptors ; Mammals ; Metabolism ; Molecular chains ; Nutrient deficiency ; Nutrition ; Oncorhynchus mykiss ; Perciformes - blood ; Perciformes - genetics ; Perciformes - metabolism ; Performance indices ; Proteins ; Receptors ; Receptors, Lipoprotein - genetics ; Receptors, Lipoprotein - metabolism ; Rodents ; Synthesis ; Transcription ; Transcription, Genetic ; Triglycerides ; Triglycerides - metabolism ; Viscera</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0129937-e0129937</ispartof><rights>2015 Yan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Yan et al 2015 Yan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-f876cffd53e70812d5dc6971b131bf1219d8a84825cf00d8c4e443d589b58def3</citedby><cites>FETCH-LOGICAL-c526t-f876cffd53e70812d5dc6971b131bf1219d8a84825cf00d8c4e443d589b58def3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482732/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482732/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26114429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Jing</creatorcontrib><creatorcontrib>Liao, Kai</creatorcontrib><creatorcontrib>Wang, Tianjiao</creatorcontrib><creatorcontrib>Mai, Kangsen</creatorcontrib><creatorcontrib>Xu, Wei</creatorcontrib><creatorcontrib>Ai, Qinghui</creatorcontrib><title>Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how dietary lipids affect lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a diet with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 10 weeks. Growth performance, plasma biochemical indexes, lipid contents and gene expression related to lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-lipid diet. In the livers of fish fed the high-lipid diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control diet, and hepatic lipid deposition increased. In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet. However, hepatic lipid content in fish fed the low-lipid diet decreased mainly due to low dietary lipid intake. In summary, findings of this study provide molecular insight into the role of lipid deposition in the liver in response to different dietary lipid contents.</description><subject>Animals</subject><subject>Aquaculture</subject><subject>Assembly</subject><subject>Catabolism</subject><subject>Catalysis</subject><subject>CD36 antigen</subject><subject>Cholesterol</subject><subject>Cholesterol, VLDL - metabolism</subject><subject>Deposition</subject><subject>Diet</subject><subject>Dietary intake</subject><subject>Education</subject><subject>Esterification</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Fish</subject><subject>Fish oils</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Laboratories</subject><subject>Larimichthys crocea</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Lipoprotein (low density) receptors</subject><subject>Lipoprotein receptors</subject><subject>Lipoproteins</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Low density lipoprotein receptors</subject><subject>Mammals</subject><subject>Metabolism</subject><subject>Molecular chains</subject><subject>Nutrient deficiency</subject><subject>Nutrition</subject><subject>Oncorhynchus mykiss</subject><subject>Perciformes - blood</subject><subject>Perciformes - genetics</subject><subject>Perciformes - metabolism</subject><subject>Performance indices</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, Lipoprotein - genetics</subject><subject>Receptors, Lipoprotein - metabolism</subject><subject>Rodents</subject><subject>Synthesis</subject><subject>Transcription</subject><subject>Transcription, Genetic</subject><subject>Triglycerides</subject><subject>Triglycerides - metabolism</subject><subject>Viscera</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUttuEzEUXCEQLYU_QGCJlyKRsr7s7QWpSilEioRU0geeLK99NnFw7MV2gvab-Qm8SVq1iCdb4zlz5nhOlr3G-QWmFf64dltvhbnonYWLHJOmodWT7BQ3lExKktOnD-4n2YsQ1nle0Losn2cnpMSYMdKcZn-uNEThBzTXvVZoDjswAc1sZ7ZgJRzhK-hd0FE7i7RFcTXiO_DIdWgu_BLQDzDG_UZT78TPhJ8nVG-0XMXVEJD0ToJ4j9oB3cBya0TUdjkqu967CEnxBiT00fnwAV2LGAd0KVPX2z4mNSSsQguvhRzM0gwSvDPo-2CTi6DD_nUqomid0WGDRNzbW3hhg_S6Hz0Lc5jrZfasEybAq-N5lt1ef15Mv07m377MppfziSxIGSddXZWy61RBocprTFShZNlUuMUUtx0muFG1qFlNCtnluaolA8aoKuqmLWoFHT3L3h50e-MCP-YUOC4bzPKGlCQxZgeGcmLN-_RXKQLuhOZ7wPklFz5qaYAnL1BUZaFwXbKuEYLSXCqAijBVF0wlrU_Hbtt2A0qCjV6YR6KPX6xe8aXbcZZGqOho5vwo4N2vLYTINzrIFKiw4LZ734Q0jOE6Ud_9Q_3_dOzASsGH4KG7N4NzPu7uXRUfd5cfdzeVvXk4yH3R3bLSv8PS8pI</recordid><startdate>20150626</startdate><enddate>20150626</enddate><creator>Yan, Jing</creator><creator>Liao, Kai</creator><creator>Wang, Tianjiao</creator><creator>Mai, Kangsen</creator><creator>Xu, Wei</creator><creator>Ai, Qinghui</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150626</creationdate><title>Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level</title><author>Yan, Jing ; Liao, Kai ; Wang, Tianjiao ; Mai, Kangsen ; Xu, Wei ; Ai, Qinghui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-f876cffd53e70812d5dc6971b131bf1219d8a84825cf00d8c4e443d589b58def3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Aquaculture</topic><topic>Assembly</topic><topic>Catabolism</topic><topic>Catalysis</topic><topic>CD36 antigen</topic><topic>Cholesterol</topic><topic>Cholesterol, VLDL - metabolism</topic><topic>Deposition</topic><topic>Diet</topic><topic>Dietary intake</topic><topic>Education</topic><topic>Esterification</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Fish</topic><topic>Fish oils</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Laboratories</topic><topic>Larimichthys crocea</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Lipoprotein (low density) receptors</topic><topic>Lipoprotein receptors</topic><topic>Lipoproteins</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Low density lipoprotein receptors</topic><topic>Mammals</topic><topic>Metabolism</topic><topic>Molecular chains</topic><topic>Nutrient deficiency</topic><topic>Nutrition</topic><topic>Oncorhynchus mykiss</topic><topic>Perciformes - blood</topic><topic>Perciformes - genetics</topic><topic>Perciformes - metabolism</topic><topic>Performance indices</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, Lipoprotein - genetics</topic><topic>Receptors, Lipoprotein - metabolism</topic><topic>Rodents</topic><topic>Synthesis</topic><topic>Transcription</topic><topic>Transcription, Genetic</topic><topic>Triglycerides</topic><topic>Triglycerides - metabolism</topic><topic>Viscera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Jing</creatorcontrib><creatorcontrib>Liao, Kai</creatorcontrib><creatorcontrib>Wang, Tianjiao</creatorcontrib><creatorcontrib>Mai, Kangsen</creatorcontrib><creatorcontrib>Xu, Wei</creatorcontrib><creatorcontrib>Ai, Qinghui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Jing</au><au>Liao, Kai</au><au>Wang, Tianjiao</au><au>Mai, Kangsen</au><au>Xu, Wei</au><au>Ai, Qinghui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-26</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0129937</spage><epage>e0129937</epage><pages>e0129937-e0129937</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how dietary lipids affect lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a diet with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 10 weeks. Growth performance, plasma biochemical indexes, lipid contents and gene expression related to lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-lipid diet. In the livers of fish fed the high-lipid diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control diet, and hepatic lipid deposition increased. In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet. However, hepatic lipid content in fish fed the low-lipid diet decreased mainly due to low dietary lipid intake. In summary, findings of this study provide molecular insight into the role of lipid deposition in the liver in response to different dietary lipid contents.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26114429</pmid><doi>10.1371/journal.pone.0129937</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-06, Vol.10 (6), p.e0129937-e0129937 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1691409262 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Aquaculture Assembly Catabolism Catalysis CD36 antigen Cholesterol Cholesterol, VLDL - metabolism Deposition Diet Dietary intake Education Esterification Fatty acids Fatty Acids - metabolism Fish Fish oils Gene Expression Genes Laboratories Larimichthys crocea Lipid Metabolism Lipids Lipoprotein (low density) receptors Lipoprotein receptors Lipoproteins Liver Liver - metabolism Low density lipoprotein receptors Mammals Metabolism Molecular chains Nutrient deficiency Nutrition Oncorhynchus mykiss Perciformes - blood Perciformes - genetics Perciformes - metabolism Performance indices Proteins Receptors Receptors, Lipoprotein - genetics Receptors, Lipoprotein - metabolism Rodents Synthesis Transcription Transcription, Genetic Triglycerides Triglycerides - metabolism Viscera |
| title | Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T06%3A51%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dietary%20Lipid%20Levels%20Influence%20Lipid%20Deposition%20in%20the%20Liver%20of%20Large%20Yellow%20Croaker%20(Larimichthys%20crocea)%20by%20Regulating%20Lipoprotein%20Receptors,%20Fatty%20Acid%20Uptake%20and%20Triacylglycerol%20Synthesis%20and%20Catabolism%20at%20the%20Transcriptional%20Level&rft.jtitle=PloS%20one&rft.au=Yan,%20Jing&rft.date=2015-06-26&rft.volume=10&rft.issue=6&rft.spage=e0129937&rft.epage=e0129937&rft.pages=e0129937-e0129937&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0129937&rft_dat=%3Cproquest_plos_%3E1692294418%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1691409262&rft_id=info:pmid/26114429&rft_doaj_id=oai_doaj_org_article_76ce5765d1864f9aa330cdee724d854d&rfr_iscdi=true |